• Animal cells and systems
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• 제10권4호
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• pp.197-201
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• 2006

Diaminopimelate decarboxylase (DAPDC, EC 4.1.1.20) catalyzes the conversion of diaminopimelate into lysine (Lys), which is the last step in Lys biosynthetic pathway. The genes for DAPDC have been reported in many bacteria, and more recently in Arabidopsis. Here we report characterization of a gene for DAPDC from rice (OsDAPDC). Sequence analysis of a cDNA clone revealed a full-length open reading frame for OsDAPDC that encoded 490 amino acids, approximately 53.2 kDa protein. The OsDAPDC protein contains a consensus binding site for pyridoxal-5'-phosphate as a cofactor and has a sequence at the amino terminus that resembles a transit peptide for localization to plastids, similar to that of Arabidopsis. Single gene encoding DAPDC was found in chromosome II in rice. The predicted amino acid sequence of OsDAPDC is highly homologous to that of the enzymes for DAPDC encoded by lysA of many bacteria. Expression of OsDAPDC in lysA mutants of Escherichia coli shows that the gene is able to functionally complement the mutants. These results suggest that OsDAPDC encodes a protein for diaminopimelate decarboxylase in rice.

• 환경생물
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• 제40권2호
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• pp.214-223
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• 2022

멸강나방(Mythimna seperata)과 혹명나방(Cnaphalocrocis medinalis)은 중국 남부 양쯔강 유역에서 봄철 편서풍을 타고 국내로 유입되는 비래 해충(Migratory insect pests)으로 벼를 기주로 삼아 벼 잎을 가해하여 생육을 저해시킨다. 두 나방의 분포를 파악하기 위해서는 서식처의 온습도 뿐만 아니라 주변 환경 요소를 파악하는 것이 중요하다. 본 연구는 두 나방의 분포를 파악하기 위해서 SDM(Species Distribution Model) 중 Machine learning model인 MaxEnt (Maximum Entropy)에 출현 자료, SSPs (Shared Socio-economic Pathways) 시나리오, RCP (Representative Concentration Pathway) 시나리오를 적용하여 현재와 미래의 서식지 적합성 모형을 예측했다. 결과로 시기에 따른 서식처 면적이 큰 차이가 없었으며, SSPs 시나리오가 나빠짐에 따라 분포 면적이 넓어졌다. 두 나방은 중국으로부터 비래 후 생존하기 위한 최적의 장소가 기주가 있는 서해안과 남해안에 집중되어 있다. MaxEnt 결과 토지피복 자료, DEM (Degital Elevation Model) 순으로 기여도가 높게 나타났다. 이는 논에서의 출현 확률 높고 고도가 높아지면서 출현 확률이 낮아졌기 때문이다. 기후 변수에서 멸강나방은 BIO_4 (Temperature seasonality), 혹명나방은 BIO_2 (Mean Diurnal Range)가 높게 나타났다. 멸강나방은 계절에 의한 기온 차가 31.9℃ 이상일 때 서식처가 줄어들고, 혹명나방은 일교차 클수록 서식처가 넓어질 것으로 나타났다. 서식지 적합성 모형에서 두 나방은 대부분의 논에서 서식이 가능할 것이라 예측되었다. 하지만, 두 나방의 출현 위치를 정확하게 예측하는 데 한계가 있으므로, 서식지 적합성 지도를 기초로 조기에 대응하는 것이 중요하다고 판단된다.

• The Plant Pathology Journal
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• 제32권3호
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• pp.228-241
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• 2016

In our previous study, we reported that a novel endophytic bacterium Bacillus oryzicola YC7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. This endophytic strain, B. oryzicola YC7007 was used as a biological control agent against bakanae disease of rice caused by Fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. Root drenching with B. oryzicola YC7007 suspension reduced the disease severity of bakanae significantly when compared with the untreated controls. The treatments of B. oryzicola YC7007 suspension ($2.0{\times}10^7cfu/ml$) to the rice rhizosphere reduced bakanae severity by 46-78% in pots and nursery box tests containing autoclaved and non-autoclaved soils. Moreover, in the detached rice leaves bioassay, the development of necrotic lesion and mycelial expansion of F. fujikuroi were inhibited significantly by spraying the culture filtrate of B. oryzicola YC7007. Drenching of ethyl acetate extracts of the culture filtrate to the rhizosphere of rice seedlings also reduced the bakanae disease severity in the plant culture dish tests. With the root drenching of B. oryzicola YC7007 suspension, the accumulation of hydrogen peroxide was observed at an early stage of rice seedlings, and a hormonal defense was elicited with and without pathogen inoculation. Our results showed that the strain B. oryzicola YC7007 had a good biocontrol activity against the bakanae disease of rice by direct inhibition, and was also capable of inducing systemic resistance against the pathogen via primed induction of the jasmonic acid pathway.

• Applied Biological Chemistry
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• 제39권6호
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• pp.424-429
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• 1996

유색미 쌀겨가 화학적 변이원에 의한 유전독성을 억제하는 작용기작을 유색미 품종인 수원415호 쌀겨의 유기용매추출물과 변이원인 mitomycin C를 이용하여 조사하였다. 70%에탄올 분획 및 클로로포름분획에서 수원 415호는 대조구로 사용된 추청에 비하여 변이원에 대한 억제활성이 월등히 높았음에도 불구하고 항산화활성은 추청보다 다소 낮은 결과로 보아 항산화작용과는 다른 억제기작이 존재함을 추정할 수 있었다. E. coli를 지시세포로 유색미 쌀겨 추출물의 mitomycin C에 대한 변이원성 억제기작을 조사한 결과, desmutagen으로 작용할 가능성이 크다는 사실을 알았고, 쌀겨 추출물과 mitomycin C를 반응시킨 후 반응액 중에 존재하는 유리상태의 mitomycin C를 정량한 결과, 유색미의 쌀겨 추출물이 mitomycin C에 직접 결합하여 변이원을 흡착함으로써 세포에 대한 유전독성을 억제할 것으로 생각되었다.

• BMB Reports
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• 제47권1호
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• pp.27-32
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• 2014

Plant abiotic stress tolerance has been modulated by engineering the trehalose synthesis pathway. However, many stress-tolerant plants that have been genetically engineered for the trehalose synthesis pathway also show abnormal development. The metabolic intermediate trehalose 6-phosphate has the potential to cause aberrations in growth. To avoid growth inhibition by trehalose 6-phosphate, we used a gene that encodes a bifunctional in-frame fusion (BvMTSH) of maltooligosyltrehalose synthase (BvMTS) and maltooligosyltrehalose trehalohydrolase (BvMTH) from the nonpathogenic bacterium Brevibacterium helvolum. BvMTS converts maltooligosaccharides into maltooligosyltrehalose and BvMTH releases trehalose. Transgenic rice plants that over-express BvMTSH under the control of the constitutive rice cytochrome c promoter (101MTSH) or the ABA-inducible Ai promoter (105MTSH) show enhanced drought tolerance without growth inhibition. Moreover, 101MTSH and 105MTSH showed an ABA-hyposensitive phenotype in the roots. Our results suggest that over-expression of BvMTSH enhances drought-stress tolerance without any abnormal growth and showes ABA hyposensitive phenotype in the roots.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.232-232
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• 2022

We reported in our recent studies that the combined treatment of Paenibacillus yonginensis DCY84^T (DCY84^T) and Silicon (Si) promotes initial plant growth and increases resistance to biotic and abiotic stress. To understand the molecular background of these phenotypes, Liquid Chromatography Mass Spectrometry (LC-MS) analysis was performed, and it was confirmed that unsaturated fatty acid metabolites such as oleic acid and linoleic acid decreased in response to the combined treatment of DCY84^T and Si. The stearoyl-acyl carrier protein desaturase (SACPD) introduces the cis double bond into the acyl-ACPs at C9, resulting in the production of unsaturated fatty acid. We identified OsSSI2 encoding SACPD in rice and found that the expression of OsSSI2 was reduced under DCY84^T and Si treatment. Furthermore, qRT-PCR analysis revealed that the expression of OsWRKY45, which is downstream of OsSSI2, was upregulated in response to DCY84^T and Si treatment. These results enable the speculation that activation of the salicylic acid (SA)-responsive gene, OsWRKY45, may contribute to enhancing biological stress resistance. Based on this, we propose a probable model for the rice defense pathway following DCY84^T and Si treatment. This model retains a WRKY45-dependent but NH1(NPR1)-independent SA signaling pathway.

• 한국지하수토양환경학회지:지하수토양환경
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• 제12권1호
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• pp.97-102
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• 2007

본 연구에서는 폐금속광산 지역에서 중금속이 인체에 미치는 위해영향을 정량적으로 평가하기 위하여 3개 금속광산 주변 지역에서 농경지 토양, 농작물 및 지하수를 채취하여 화학분석을 실시하였다. 이들 폐금속광산 주변에 야적된 광산폐기물(광미)은 바람이나 집중 강우에 의해 광미댐 하부에 있는 농경지나 하천으로 유입되어 주변 환경을 오염시킬 가능성이 크다. 따라서 폐금속광산에서의 인체노출경로는 농사활동을 통한 토양의 섭취, 지하수(식수)의 섭취, 쌀의 섭취, 농사활동에 의한 토양의 피부접촉 등 4가지로 파악할 수 있었다. 각 노출경로별 비발암성위해도 평가 결과, 모든 광산에서 쌀의 섭취를 통한 중금속의 독성위해도가 가장 높게 나타났다. 광산별 4가지의 인체노출경로를 총합한 HI 지수가 3개 광산 모두 9.0 이상을 나타내었으며, 특히 B 광산이 HI 29.2로 중금속에 의한 독성위해도가 가장 높은 것으로 판명되었다. 비소에 대한 발암위해도 평가 결과, 3개의 폐금속광산 지역에서 쌀 섭취의 노출경로를 통한 비소에 의해 암이 발생할 확률이 천명중의 1명 이상으로 높게 나타났다. 이는 환경부 토양오염 위해성평가지침(안)과 미국 EPA에서 제시한 허용가능한 초과발암위해도보다도 크므로 이들 지역 주민들이 비소에 의해 오염된 농작물(쌀)을 지속적으로 장기간 섭취하게 된다면 비소가 건강에 미치는 위해영향이 크다고 판단된다.

• Journal of Radiation Protection and Research
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• 제25권1호
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• pp.21-30
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• 2000

벼의 방사성 핵종 직접오염 경로를 분석하기 위하여 동위원소 실험온실내에서 $^{54}Mn,\;^{57}Co,\;^{85}Sr,\;^{134}Cs$의 혼합용액을 벼의 생육중 여섯 차례에 걸쳐 작물체 지상부에 처리하였다. 작물체 차단계수는 핵종 간에 차이가 없었고 처리시기가 수확기에 가까울수록 증가하여 최고 약 0.94에 달리하였다. 작물계에 침적한 각 핵종의 수확시 잔류율은 방사능 붕괴가 없다고 가정할 예 처리시기에 따라 각각 $19{\sim}47%,\;17{\sim}43%,\;19{\sim}42%,\;23{\sim}61%,\;11{\sim}69%$였다. 종실 전류계수는 처리시기에 따라 각각 $6.9{\times}10^{-4}{\sim}3.8{\times}10^{-2},\;3.6{\times}10^{-3},\;1.6{\times}10^{-1}{\sim}5.8{\times}10^{-4}{\sim}3.2{\times}10^{-2},\;1.6{\times}10^{-4}{\sim}7.6{\times}10^{-5},\;3.2{\sim}10^{-2}{\sim}2.0{\times}10^{-1}$의 범위였고 모두 종실의 발육성기 처리시 가장 높았다. 강우 빈도의 차이가 2배 이내일 때는 강우빈도가 잔류율과 전류계수에 큰 영향을 미치지 않는 것으로 나타났다. 본 연구결과는 벼의 생육중 사고침적시 쌀알 내 핵종농도 예측에 활용될 수 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.109-109
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• 2017

The core components of ABA-dependent gene expression signaling have been identified in Arabidopsis and rice. This signaling pathway consists of four major components; group A OsbZIPs, SAPKs, subclass A OsPP2Cs and OsPYL/RCARs in rice. These might be able to make thousands of combinations through interaction networks resulting in diverse signaling responses. We tried to characterize those gene functions using transient gene expression for rice protoplasts (TGERP) because it is instantaneous and convenient system. Firstly, in order to monitor the ABA signaling output, we developed reporter system named pRab16A-fLUC which consists of Rab16A promoter of rice and luciferase gene. It responses more rapidly and sensitively to ABA than pABRC3-fLUC that consists of ABRC3 of HVA1 promoter in TGERP. We screened the reporter responses for over-expression of each signaling components from group A OsbZIPs to OsPYL/RCARs with or without ABA in TGERP. OsbZIP46 induced reporter most strongly among OsbZIPs tested in the presence of ABA. SAPKs could activate the OsbZIP46 even in the ABA independence. Subclass A OsPP2C6 and -8 almost completely inhibited the OsbZIP46 activity in the different degree through the SAPK9. Lastly, OsPYL/RCAR2 and -5 rescued the OsbZIP46 activity in the presence of SAPK9 and OsPP2C6 dependent on ABA concentration and expression level. By using TGERP, we could characterize successfully the effects of ABA dependent gene expression signaling components in rice. In conclusion, TGERP represents very useful technology to study systemic functional genomics in rice or other monocots.

• Journal of Plant Biotechnology
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• 제38권1호
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• pp.77-86
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• 2011

Alcohol dehydrogenase (E.C.1.1.1.1) is an enzyme present in higher plants involved in the anaerobic fermentation pathway that catalyzes the reduction of pyruvate to ethanol, resulting in continuous $NAD^+$ regeneration. It also plays an important role in many plant developments including tolerance to anoxia condition. Here, a cDNA clone encoding alcohol dehydrogenase (ADH) was isolated from Chinese cabbage (Brassica rapa) seedlings. The gene named Bradh1 had a total length of 1,326 bp that contains a single open reading frame of 1,140 bp. The predicted protein consists of 379 amino acid residues with a calculated molecular mass of 41.17 kDa. Expression pattern analysis revealed a tissue-specific expressing gene in different tissues and strongly expressed in the shoot, roots and seeds of Chinese cabbage. Agrobacterium transformation of full-length cDNA Bradh1 into rice Gopumbyeo showed high efficiency. Furthermore, induction of ADH in transgenic rice enhanced tolerance to anaerobiosis stresses and elevated mRNA transcripts. The overexpression of Bradh1 in rice increases germination under anaerobiosis stresses, implying the possibility of developing new varieties suited for direct seeding or flood-prone rice field.