• Journal of Life Science
• /
• v.25 no.10
• /
• pp.1091-1097
• /
• 2015

We have isolated and characterized an ascorbate peroxidase (APx) gene, OsAPx1 from rice. Northern and Western blot analyses indicated that at young seedling stage, OsAPx1 mRNA was expressed highly in root, shoot apical meristem (SAM) and leaf sheath than leaf. In mature plant, OsAPx1 gene expressed highly in root, stem and flower but weakly in leaf. OsAPx1 gene and protein expression level was induced in leaves inoculated with Magnaporthe oryzae (M. oryzae) and Xanthomonas oryzae pv. oryzae (Xoo). Phytohormones treatment showed that OsAPx1 was up-regulated by jasmonic acid (JA), but was down regulated by ABA and SA co-treatments with JA, resulting that they have antagonistic effect on pathogen responsive OsAPx1 expression. Phylogenetic analysis illustrated that Arabidopsis AtAPx1 has a close relationship with OsAPx1. In AtAPx1 knock out lines, the accumulation of O₂^- and H₂O₂ are all highly detected than wild type, revealing that the high concentration of exogenous H₂O₂ cause the intercellular superoxide anion and hydrogen peroxide accumulation in AtAPx1 knockout plant. These results suggested that OsAPx1 gene may be associated with the pathogen defense cascades as the mediator for balancing redox state by acting ROS scavenger and is associated with response to the pathogen defense via Jasmonic acid signaling pathway.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.2 no.2
• /
• pp.24-32
• /
• 1999

To develope appropriate soil base for planting on the artificial ground, 9 kinds of soil types(varying the components and mixing ratios) and 3 levels of soil depths(10cm, 15cm, 20cm) were chosen. And their plant growing effects were tested and analysed from the test plant Zoysia japonica. The results of the research are as follows. 1. Among the 9 type of soil mixtures, the "sandy loam" soil type gave the worst effects on germination, disease contamination and ground covering. 2. The soil types like VSH(vermiculite20%+sand70%+humus sawdust10%), VSS(vermiculite 40%+sand 50%+humus sawdust 10%) and VS(vermiculite 70%+sand 30%), where vermiculite and sand were added to, show better germination effect promoted from the better condition of aeration and saturation. 3. The plant growing effects(leaf length and ground covering ratio) was evident under the soil types like VSH(vermiculite20%+sand70%+humus sawdust10%) and VSS(vermiculite40%+sand50%+humus sawdust10%), where organic matters were added to. 4. Vermiculite added soil types effect fast leaf decolorization on the tested Zoysia japonica plant, on the contrary to organic matter mixed soil types including SCS(sandy loam 50%+carbonized rice husk30%+sand20%) and SHS(sandy loam 50%+humus sawdust30%+sand20%) with which green leaves subsist longer. S. Soil depth effect to plant growth was found. And a favorable covering rate was accomplished even at the soil depth of 15cm - the limit soil depth for grass survival - from the soil types where organic matters were mixed to. From this result, the soil depth limit for plant survival could be said to be shall owed if appropriate soil type were based.

• Horticultural Science & Technology
• /
• v.32 no.6
• /
• pp.872-878
• /
• 2014

This study aimed to enhance storage and freshness of strawberry fruits and foliage vegetables by spray treatment with Chlorella vulgaris as a bio-fertilizer. The tested strain, C. vulgaris CHK0008, was isolated from an organically cultivated rice paddy and identified as C. vulgaris by its morphology and 18S rDNA and 23S rDNA sequence homology. We successfully cultured C. vulgaris CHK0008 in BG11 modified medium (BG11MM) and adjusted $2.15{\times}10^6cell/mL$ C. vulgaris CHK0008 to one OD value by measuring the optical density at 680 nm using a UV-vis spectrophotometer. The soluble solid content of 'Seolhyang' and 'Yukbo' strawberry fruits treated by spray application with C. vulgaris CHK0008 was enhanced by 22.2% and 11.5% respectively, compared to untreated controls. Additionally, the decay rates of treated 'Seolhyang' and 'Yukbo' strawberry fruits decreased 63.8% and 74.4% respectively, compared to untreated control. Surface color changes and chlorosis of leaves in leaf vegetables such as lettuce, kale, red ornamental kale, white ornamental kale and beet were observed in samples treated with water spray for 10 days after cold storage. However, the decay rate of leafy vegetables treated with foliar application of 25% C. vulgaris CHK0008 liquid culture was significantly decreased compared to that of the untreated control during storage at $4^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.55 no.3
• /
• pp.187-194
• /
• 2010

The soybean stay green mutant genotype (SSG) derived from the nuclear gene, d1d2, and cytoplasmic gene, cytG, inhibit the breakdown of chloroplast in the leaves, pod walls, seed coats, and embryos during maturity. Soybean seed with black seed coat and green cotyledon (SBG) are preferred than black seed coat with yellow cotyledon (SBY) especially for cooking with rice and as source of traditional food in Korea. The researchers evaluated the seed's chlorophyll content of SSG and introduced SSG to the SBG variety breeding program. The seed chlorophyll content of SSG with d1d2 was $39.93{\sim}60.80\;{\mu}g/g$ and SSG with cytG $38.08{\sim}39.89\;{\mu}g/g$. The Korean SBG variety which was derived from SSG with cytG, contains $16.35{\sim}37.73\;{\mu}g/g$. The composition of seed chlorophyll differs according to the genetic background of SSG genotype. Inheritance study showed that cotyledon color was segregated 15:1 (yellow:green) at $F_2$ seed indicating two recessive genes control green cotyledon as revealed by previous study. Only less than 3% soybean lines showed black seed coat with green cotyledon among crosses SBY and SSG (d1d2). Results showed that SSG with d1d2 can be used as a good source for SBG with high chlorophyll content in the seed cotyledon, but due to the complex genetic behavior, breeding resource of SBG with d1d2 should be prepared to improve the breeding efficiency for development SBG variety.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.37 no.2
• /
• pp.125-131
• /
• 2017

'Saeyoung', a winter triticale (X Triticosecale Wittmack) for forage, was developed at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2012. The cultivar 'Saeyoung' has narrow and long leaves of light green color, middle size and thin culm, and a medium grain of brown color. The heading date and yellow ripe stage of 'Saeyoung' was May 3 and May 27, which were similar to check cultivar 'Shinyoung', respectively. 'Saeyoung' showed a little stronger in cold tolerance and a little weaker in resistance to lodging than the check, and wet injury, powdery mildew, and leaf rust were similar to those of the check cultivar. The forage fresh and dry matter yields of 'Saeyoung' at milk-ripe stages were 47.2 and $15.6MT\;ha^{-1}$, respectively, which was 9% and 4% higher than those of the check. The crude protein content of 'Saeyoung' was 0.4% lower than 6.8% of the check, while was higher than the check cultivar 'Shinyoung' in neutral detergent fiber, acid detergent fiber. Total digestible nutrients of 'Saeyoung' was also 3% lower than 62.8% of the check cultivar. It showed grain yield of $4.1MT\;ha^{-1}$, which was 11% higher than that of the check. 'Saeyoung' is recommended for fall sowing forage crops in areas in which average daily minimum mean temperatures in January are higher than $-10^{\circ}C$.

• Korean Journal of Plant Resources
• /
• v.8 no.3
• /
• pp.325-334
• /
• 1995

The early senescence mutant induced from Gihobyeo by $\gamma-ray$ irradiation was determined. The mutated gene expression was identified with comparing the characteristic of original cultivar. The mutant had so similar the morphological characteristics to original cultivar that it couldn't be distinguished until senescence occurred at about 20 days after heading. Suddenly yellow leaves were observed within a few days due to great decreases in total chlorophyll and various carotenoid contents. Transmission electron microscopy showed the formation of starch granules, distortion of fine structure of leaf cell organelles, especially grana structures, and the decrease in grain filled after senescence occurred. But banding patterns of total proteins and isozymes have not show any differences, The early senescence mutant will be very useful for study material not only on physiological and biochemical properties of plant senescence but also on gene expression regulating senescence which gives great influence on yield potential and its stability.

• Korean journal of food and cookery science
• /
• v.25 no.6
• /
• pp.690-702
• /
• 2009

Liparis tessellatus(cubed snailfish), Hemitripterus villosus(shaggy sea raven), Ligularia fischeri, and Cirsiu setidens, which are staple products in Samcheok, were studied as food resources for the development of a representative local menu. Thus, their nutritional compositions were analyzed, and the resulting values were used to assess the nutritional characteristics of the newly-developed menu. In Liparis tessellatus and Hemitripterus villosus, the organic components including protein and fat tended to be lower than the corresponding values referred to as the 'Seventh Revision Food Composition Table' (2006), which was presumably linked to the spawning period of the fish. The contents of Zn, Cu, and Mg, which are nutritionally important even though the consumption of these minerals by Koreans is not sufficient, were determined to 0.5, 0.2, and 34.3 mg/100 g for Ligularia fischeri, and 26.2, 1.1, and 147.7 mg/100 g for dried Cirsiu setidens. Cirsiu setidens cultivated in Samcheok contained noticeably higher amounts of calcium, zinc, and iron, and its calcium level was more than an order of magnitude higher than that provided by the Food Composition Table. This might be associated with the fact of that Samcheok is abundant in lime which consists of calcium-containing inorganic materials. n-3 fatty acids including docosahexaenoic acid(DHA) accounted for 21% and 13% of the total fatty acids, for Liparis tessellatus and Hemitripterus villosus, respectively, and the major amino acids were glutamic acid, aspartic acid, glycine, and alanine for both fish. One of the newly developed menus, Cirsiu setidens hae-jang-gook, was nutritionally superior to the corresponding menu that used dried radish leaves, a typical ingredient in hae-jang-gook, in terms of the protein, iron, and zinc contents. Rice pottage cooked with Liparis tessellatus instead of abalone was high in protein, calcium, iron and zinc, where one serving provided 29.1, 15.4, 31.0, and 27.8% of their Reference Intake (RI)s, respectively.

• Journal of Bio-Environment Control
• /
• v.20 no.4
• /
• pp.352-356
• /
• 2011

It is true that the industrial development has usually been accompanied with urbanization or centralization of population that has inevitably led to high-rise buildings and densely built-up living area in the cities. While it is badly needed to acquire as much green land within the city limits as possible to compensate for reduced space for recreational purpose in parallel with increasingly urbanized area, the living conditions of plants have become seriously devastated due to shortage of sun light walled-off by high-rise buildings and contaminated environment and air. The shade that is generated by high-rise and compact buildings hinders growth of plants, which makes it urgent to develop native ground cover plant that is strongly viable in the shade. For this purpose, Ligularia stenocephala, best known as greens for Ssam (rice and condiments wrapped in leaves) was cultivated under the 30%, 50%, and 80% shadings and observed to see if there would be any changes in soil conditions, growth of plants and chlorophyll contents depending on the shading rate. The leaf number was 10.8 pieces under the 50% shading and 8.4 under the 30%-shading, 7.7 pieces more than that cultivated under lighting. The leaf width turned out to be excellent from cultivation under the 50%- shading, an evidence indicating its possibility of being cultivated as native ground cover plant in the shade. The live weight of the plants cultivated under the shading increased to 31.63 g, 43.39 g and 19.40 g, respectively, compared to 90.43 g of those in the untreated control plot. The increase in growth of roots was particularly significant with 48.48 g in comparison to 12.33 g under 30% shading cultivation. The chlorophyll synthesis amounted to 46.2 under the 50% shading, showing an increase compared to 41.9 under lighting. The chlorophyll synthesis rather shrank under other shading conditions. The cultivation of Ligularia stenocephala under the 50% shading showed the best condition in growth as native ground cover plant.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.36 no.2
• /
• pp.142-149
• /
• 2016

'Shinseong', a winter forage triticale cultivar (X Triticosecale Wittmack), was developed at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2015. The cultivar 'Shinseong' was selected from the cross RONDO/2$^*$ERIZO_11//KISSA_4/3/ASNO/ARDI_3//ERIZO_7 by CIMMYT (Mexico) in 1998. Subsequent generations were handled in pedigree selection programs at Mexico from 1999 to 2004, and a line 'CTSS98Y00019S-0MXI-B-3-3-5' was selected for earliness and good agronomic characteristics. After preliminary and advance yield testing in Korea for 3 years, the line was designated 'Iksan47'. The line was subsequently evaluated for earliness and forage yield in seven locations, Jeju, Iksan, Cheongwon, Yesan, Gangjin, Daegu, and Jinju from 2013 to 2015 and was finally named 'Shinseong'. Cultivar 'Shinseong' has the characteristics of light green leaves, yellow culm and spike, and a medium grain of brown color. The heading date of cultivar 'Shinseong' was April 24 which was 3 days earlier than that of check cultivar 'Shinyoung'. The tolerance or resistance to lodging, wet injury, powdery mildew, and leaf rust of 'Shinseong' were similar to those of the check cultivar. The average forage dry matter yield of cultivar 'Shinseong' at milk-ripe stages was $15MT\;ha^{-1}$, which was 3% lower than that ($15.5MT\;ha^{-1}$) of the check cultivar 'Shinyoung'. The silage quality of 'Shinseong' (6.7%) was higher than that of the check cultivar 'Shinyoung' (5.9%) in crude protein content, while was similar to the check cultivar 'Shinyoung' in acid detergent fiber (34.6%), neutral detergent fiber (58.6%), and total digestible nutrients (61.6%). It showed grain yield of $7.2MT\;ha^{-1}$ which was 25% higher than that of the check cultivar 'Shinyoung' ($5.8MT\;ha^{-1}$). This cultivar is recommended for fall sowing forage crops in areas in which average daily minimum mean temperatures in January are higher than $-10^{\circ}C$.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.29 no.3
• /
• pp.41-48
• /
• 2021

This study aimed to develop an alternative organic fertilizer to castor oil cake-based fertilizers. To assess the nutrient effect of the developed fermented mixed organic fertilizers, the yield of lettuce and soil characteristics after growth were analyzed and compared to those of a trial using a mixed expeller cake fertilizer. Two fermented mixed organic fertilizers, FA and FB, each containing 5.0% nitrogen, 2.6% phosphate, and 1.4% potassium, were produced by mixing different ratios of rice bran, dried distillers grains, sesame oil meal, and fish meal. This study was conducted with six trials: untreated, mixed expeller cake fertilizer, and the fermented mixed organic fertilizers FA and FB. Based on the amount of nitrogen fertilization (70 kg ha^-1) on the lettuce, the fermented mixed organic fertilizers FA and FB were applied at 100% and 150%, respectively, and the mixed oil cake was applied at 100%. As the amount of treatment increased, there was no significant difference except the number of leaves in FA treatment. The yields from the FA100 and FB100 treatments were 38.2 and 40.8 Mg ha^-1, respectively, which was not significantly different from that of the mixed expeller cake fertilizer treatment at 38.3 Mg ha^-1. In addition, the nitrogen uptake and utilization efficiency of the lettuce were not significantly different between mixed expeller cake fertilizer and fermented mixed organic fertilizer treatments. Analysis of the chemical properties of the soil after the trial showed that he mixed expeller cake fertilizer treatment showed the lowest pH. There were no significant differences in electrical conductivity, content of soil organic matter, available phosphate, and exchangeable cation among the fertilizer treatments. However, the bacterial and actinomyces density was higher in the soil from the fertilizer trials than in the non-fertilizer trials. These results indicated that the two tested fermented mixed organic fertilizers had nourishing effects and soil characteristics that were similar to those of the mixed expeller cake fertilizer. Thus, farmers can use these fermented mixed organic fertilizers as alternatives to castor oil cakes for the cultivation of organic lettuce.