• 한국식품과학회지
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• 제28권6호
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• pp.1001-1008
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• 1996

우리나라의 세균성 식중독의 주된 원인은 식중독성 황색포도상구균에 의한다. 따라서 식품공전에는 도시락, 빵, 떡으로부터는 이 미생물이 검출되지 않도록 규정되어있다. 현행의 황백포도상구균의 검사 방법은 적어도 5일 이상을 요하므로 이 식중독에 대한 대처가 미흡한 설정이다. 이 연구에서는 식중독성 황색포도상구균의 검출법을 중합효소 연쇄반응을 이용하여 개선하였다. 이 반응을 위해 황색포도상구균의 장도속도를 암호하는 유전자를 유형별로 증폭할 수 있는 시발물질들을 고안하였다. 한 황색포도상구균으로부터 중합효소 연쇄반응에 적합한 DNA를 신속하게 추출하는 방법을 개발하였다. 중합효소 반응조건은 다음과 같다. 반응액의 총부피, $(50\;{\mu}l)$; 표적 DNA, $2\;{\mu}l$ (약 20ng); 10배 진한 반응완충액, $5\;{\mu}l$; 시발물질, 100pmole; dNTP (10 mM), $4\;{\mu}l$; Taq DNA 중합효소, 205단위, 작동조건은 변성전, $94^{\circ}C$-5분; 변성, $94^{\circ}C$ 15초; 냉각, $50^{\circ}C$15초; 연장, $72^{\circ}C$ 20초; 연장후, $72^{\circ}C$-5분이었으며 변성-냉각-연장을 30회 되풀이하였다. dlj한 검출조건으로 황색포도상구균을 5시간 이내에 독소 유형별로 확인할 수 있었으며 이를 시판되는 떡과 빵에 적용하여 이 미생물의 존재를 확인하였다.

• Nutrition Research and Practice
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• 제13권3호
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• pp.230-239
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• 2019

BACKGROUND/OBJECTIVES: Little is known about the dietary patterns (DPs) of women during pregnancy. The present study aimed to identify the DPs of pregnant Malaysian women and their associations with socio-demographic, obstetric, and anthropometric characteristics. SUBJECTS AND METHODS: This prospective cohort study included 737 participants enrolled in Seremban Cohort Study between 2013 and 2015. Food consumption was assessed using a validated 126-food item semi-quantitative food frequency questionnaire (SFFQ) at four time-points, namely, pre-pregnancy and at each trimester (first, second, and third). Principal component analysis (PCA) was used to identify DPs. RESULTS: Three DPs were identified at each time point and designated DP 1-3 (pre-pregnancy), DP 4-6 (first trimester), DP 7-9 (second trimester) and DP 10-12 (third trimester). DP 1, 4, and 7 appeared to be more prudent diets, characterized by higher intakes of nuts, seeds & legumes, green leafy vegetables, other vegetables, eggs, fruits, and milk & dairy products. DP 2, 5, 8, and 11 had greater loadings of condiments & spices, sugar, spreads & creamer, though DP 2 had additional sweet foods, DP 5 and 8 had additional oils & fats, and DP 11 had additional tea & coffee, respectively. DP 3 and 6 were characterized by high protein (poultry, meat, processed, dairy, eggs, and fish), sugars (mainly as beverages and sweet foods), and energy (bread, cereal & cereal products, rice, noodles & pasta) intakes. DP 9 had additional fruits. However, DP 12 had greater loadings of energy foods (bread, cereal & cereal products, rice, noodles & pasta), sugars (mainly as beverages, and sweet foods), and good protein sources (eggs, nuts, seeds & legumes). Malays were more likely to have lower adherence (LA) for DP 1 and 10 than non-Malays. DP 2, 8, and 11 were more prevalent among Malays than non-Malays. Women with a higher education were more likely to have LA for DP 10, and women with a greater waist circumference at first prenatal visit were more likely to show LA for DP 11. CONCLUSIONS: DPs observed in the present study were substantially different from those reported in Western populations. Information concerning associations between ethnicity, waist circumference and education with specific DPs before and throughout pregnancy could facilitate efforts to promote healthy dietary behavior and the overall health and well-being of pregnant women.

• 한국식품조리과학회지
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• 제15권1호
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• pp.16-22
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• 1999

초등학교 아동 211명을 대상으로 식품섭취실태가 치아우식에 미치는 영향을 조사한 결과는 다음과 같다 1. 전체 조사 대상자의 65.4%가 치아우식 아동이었고, 간식은 전체 아동의 83.9%가 섭취하였으며, 간식을 하는 아동의 치아 우식율이 간식을 하지 않는 아동의 우식율보다 유의적으로 높았다(p＜0.001). 2. 1일 양치질 횟수는 전체의 38.4%가 1회, 61.6%가 2회 이상이었으며, 양치횟수 2회 이상인 아동과 식사후에 양치하는 아동에서 치아 우식율이 낮은 경향이었다. 3. 주식으로 자주 섭취하는 음식은 양호한 아동이 밥33.30%, 라면 14.81%, 빵 9.57%, 떡 6.16%, 국수 4.88%이었으며, 치아우식인 아동은 밥 27.04%, 떡 12.04%, 라면 8.50%, 빵 8.11%, 볶음밥 5.49% 순이었다. 4. 부식으로 자주 섭취하는 음식은 양호한 아동이 김치가 13.88%로서 가장 높았으며, 다음으로 계란 6.21%, 김 5.51%, 어묵 5.51%, 생선 5.03%순이었으며, 콩자반이 0.44%로서 가장 낮았다. 치아우식인 아동은 감자국이 6.86%로서 가장 높았으며 다음으로 오이 6.56%, 콩나물국 5.78%, 두부 5.33%, 깻잎 5.21% 순이었고, 상치가 1.23%로서 가장 낮았다. 5. 간식의 섭취율은 양호한 아동에서 아이스크림이 9.85%로서 가장 높았으며, 다음으로 비스킷 7.35%, 껌6.17%, 밤 6.16%, 사과 5.81%, 우유 5.56%, 요구르트4.86%, 어포 4.39%, 사탕 4.15%, 초콜릿 3.91% 순이었으며, 치아우식인 아동에서는 비스킷이 10.00%로서 가장 높았으며, 다음으로 아이스크림 6.75%, 사탕5.88%, 과일통조림 5.70%, 우유 5.41%, 옥수수 5.00%, 바나나 5.00%, 땅콩 4.42%, 튀김 4.39% 순이었다. 6. 부식의 우식성식품ㆍ청정식품ㆍ보호식품의 섭취율은 양호한 아동이 각각 4.0%, 40.8%, 55.1%이었으며, 치아우식인 아동은 11.5%, 43.9%, 보호식품 44.6%로서 치아우식인 아동이 양호한 아동보다 우식성식품을 자주 섭취하는 반면에 보호식품을 적게 섭취하였다.(p<0.001). 7.간식의 우식성식품ㆍ청정식품ㆍ보호식품의 섭취율은 양호한 아동이 각각 67.8%, 10.4%, 21.7%이었으며, 치아우식인 아동은 75.3%, 8.8%, 15.9%로서 치아우식 아동의 우식성식품 섭취율이 높았다(p＜0.001). 이상의 결과를 종합해 볼 때 아동들의 부식에서는 청정식품과 보호식품의 섭취율이 우식성식품보다 높았으나 간식에서 우식성식품의 섭취율이 청정식품과 보호식품보다 2∼3배나 높아 간식으로 인한 치아우식의 문제가 더 클 것으로 사료된다. 특히 치아우식 아동이 양호한 아동보다 부식과 간식에서 치아우식 유발지수가 높은 우식성식품을 많이 섭취하는 것으로 나타나 치아우식증을 예방하기 위한 식이조절법으로 치아우식 유발지수가 낮고 당질 함량이 적은 식품과 채소류나 과실류 등의 청정식품 및 칼슘과 단백질이 풍부한 보호식품을 많이 섭취하도록 권장하여야 하며, 한정되어 있는 간식을 다양하고 균형있게 선택할 수 있도록 하는 영양 교육이 실시되어야 할 것으로 사료된다

• 한국식품영양학회지
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• 제26권4호
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• pp.952-966
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• 2013

The purpose of this study is to analyze the differences of food habits and food preferences between lower and higher grade students through the survey of elementary school students in Daejeon four elementary schools located in Jung-Gu and Seo-Gu in Daejeon are selected for this survey randomly and the completion of the questionnaires are according to grade. 1. Practical Analysis of Food Habits: For regular meals, the highest number of subjects skipped breakfast. It is being found that the girl students (81.61%) consumed more cooked rice than the boys (78.85%) and more boys (13.10%) ate bread or milk more than the girls (9.20%). The most important thing for the lower grades students (34.20%) when consuming is the nutritious foods, while the higher grades students (49.67%) consumed the set meals. During consumption, the highest number of the subjects watched TV or read books from time to time. More higher grades students (38.98%) preferred spicy and salty foods than the lower grades (22.09%). 2. Food Preferences: Between rice and noodles, most subjects preferred cooked rice (81.38%), Jajangmyeon (80.80%), Ramyeon (79.43%), and disliked bean-mixed rice (28.39%). For soups, Most students enjoy the Deokkguk. Majority of the subjects prefer meat and fried or steamed foods, and also grilled and pan-fried dishes. For the pan-roasted and fried dishes, most subjects did not enjoy the rhizome fry and sweet-pumpkin fry methods, and for vegetables, they did not like wild vegetable and seasoned cucumber (40.57%) The higher grades students enjoy the vegetables and salads more than the lower grades students. For Kimchi varieties, it is found that subjects like the cabbage Kimchi (69.66%) the most. There is little difference in food preference between the lower and higher grade students, but the rankings of food preferences are different according to various grades.

• Journal of Microbiology and Biotechnology
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• 제19권5호
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• pp.468-473
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• 2009

The antibiotic meroparamycin was produced in the free culture system of Streptomyces sp. strain MAR01. Five solid substrates (rice, wheat bran, Quaker, bread, and ground corn) were screened for their ability to support meroparamycin production in solid-state fermentation. In batch culture, wheat bran recorded the highest antibacterial activity with the lowest residual substrate values. The highest residual substrate values were recorded for both ground corn and Quaker. On the other hand, no antibacterial activity was detected for rice as a solid substrate. The use of the original strength of starch-nitrate medium in the solid-state fermentation gave a lower antibacterial activity compared with the free culture system. Doubling the strength of this medium resulted in the increase in the activity to be equivalent to the free culture. The initial pH (7.0) of the culture medium and 2 ml of spore suspension (1 ml contains $5{\times}10^{9}spores/ml$) were the optima for antibiotic production. The water was the best eluent for the extraction of the antibiotic from the solid-state culture. Ten min was enough time to extract the antibiotic using a mixer, whereas, 60 min was required when shaking was applied. Semicontinuous production of meroparamycin using a percolation method demonstrated a more or less constant antibacterial activity over 4 runs ($450-480{\mu}g/ml$). The semicontinuous production of the antibiotic was monitored in a fixed-bed bioreactor and the maximum activity was attained after the fourth run ($510{\mu}g/ml$) and the overall process continued for 85 days.

• Journal of Nutrition and Health
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• 제39권5호
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• pp.460-466
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• 2006

To investigate the effects of dietary patterns on bone mineral density and its biochemical markers among Korean healthy college women for 2 years, 34 female college students were recruited through convenience sampling. Bone mineral density was measured using Dual Energy X-ray Absorptiometry (DEXA) twice at baseline and two years later. Osteocalcin and parathyroid hormone were measured in fasting serum and N-teleopeptides of type collagen (NTx) in urine. Dietary intake was assessed by 24-hour recall method 8 times with average 4-month interval. Dietary patterns with percent energy of each food group using cluster analysis were classified into two groups. The first cluster (n = 16) was characterized with high consumption of bread, snack, fast foods, beverage and considerable of rice so it was determined as 'Modified dietary pattern group'. The second cluster was characterized with high consumption of rice and kimchi so determined as 'Traditional dietary pattern group'. There were no significant difference of age, menarcheal age, body mass index but percent of body fat by pattern groups. The traditional group showed higher value of bone mineral density among lumber spine and all femur sites at baseline and 2 years later but it was not significant after adjusted for percent of body fat. Serum osteocalcin and urine NTx was higher among the traditional group at baseline than the modified group. There were similar proportions of carbohydrate:fat:protein between groups but significantly higher intake of protein, iron, vitamin A among the traditional group. In conclusion, there were two distinctive dietary patterns among Korean college women. There was difference of bone mineral density and its biochemical markers between two patterns. Further research would be necessary to explore the relationship between dietary patterns and health risks for larger-sized and various populations.

• 한국식생활문화학회지
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• 제10권5호
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• pp.391-404
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• 1995

This study was carried out to investigate the differences of the dietary behavior of the college students by the type of the residence, that is, home-living, dormitory-living, and self-cooking. The responses of 307 students to the questionaire were analyzed. The average meal frequency of the students was 2.4 times a day. Breakfast was skipped most frequently, especially in dormitory-living and self-cooking students compared to home-living ones. Forty-three percent of the students were found to eat breakfast less than twice a week. Such a poor eating habit was mainly due to insufficient time or idleness. When the quality of the meal was evaluated, dormitory-living and self-cooking students had worse scores than home-living ones. The formers had poor dietary habit in terms of balanced breakfast and food diversity of each meal compared to the latter. Fast foods and coffee were consumed more frequently by dormitory-living and self-cooking students than home-living ones. The main food they eat was rice. However, female dormitory-living students had a higher tendency to enjoy bread for breakfast and noodle for dinner compared to the other groups. Home-living and self-cooking students had snack more frequently than dormitory-living ones, and female than male. In terms of snack time, most of the respondents had snack between lunch and dinner or after dinner. The percentage of those who had snack after dinner was greater in dormitory-living and self-cooking students than home-living students. Results of the food preference test showed that their favorite dishes were fruit salad, Bulgogi, uncurdled Tofu stew, Kimbab, and boiled rice. We observed that dormitory-living and self-cooking students had more problems in their dietary behavior. Therefore, they need to learn how to manage their own meals. This study might be helpful to develop nutrition education materials for the dietary improvement of the college students.

• Preventive Nutrition and Food Science
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• 제3권1호
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• pp.77-84
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• 1998

To evaluate food consumption patterns of first generation Korean-American in Hawaii, questionnaires were developed using sociodemographic questions and food frequency questionnaire, which included 139 food items most often consumed among Korean foods and American foods. The questionnaires surveyed 157 first generation Korean-Americans in Hawaii. Mean daily servings for the first generation Korean-Americans were calculated for 139 food items combined into 41 food groups based on similarity in nutrient composition and serving size. The food groups which were consumed in amounts over one serving per day for all subjects were rice, Kimchi , non-citrus fruit , vegetables, organge/green vegetables. oil. margarine and coffee/tea. All subjects consumed less than one serving of hotdogs, hamburgers, pizza and pancakes per week(0.14 serving per day). The most notable characteristic of food consumption for first generation Korean-Americans was that they consumed more Korean food such as rice, Kimchi, soybean paste(Deenjang), soybean curd and seaweed than American foods. Compared with other groups based on age and gender, younger men showed significantly(p<0.05) more frequent consumption of beef/pork, sausages /hams /bacons and hambergers. Older men were significantly(p<0.05) more likely to consume Doenjang and less likely to consume pizza and hamburgers. Daily servings were below the recommended level for thegrains /bread/cereals group and fats/oils/sweets group for all subjects . Fruits/vegetables group servings exceeded the recommeded 5 daily servings for younger men. In correlations of daily servings of selected foods among Korean foods and American food with sociodemographic characteristics, this study showed that the older the subjects and the shorter the stay in Hawaii, subjects were more likely to consume Kroean foods.

• 한국식생활문화학회지
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• 제11권5호
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• pp.621-634
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• 1996

This study was conducted to investigate the eating patterns of Korean men by analyzing the relation among their socio-demographic characteristics, value toward food and nutrition, and eating behavior. Nine hundred twenty nine Korean men were selected by the stratified random sampling method. The socio-demographic characteristics of the subjects had a significant influence on their value toward food and nutrition. After in their sixties had meals for physiological needs rather than for nutrition or preference. Highly educated people, professional workers, office workers and people in the higher incomes, placed a higher value toward food and nutrition. In addition, rural inhabitants had a significantly lower value toward food and nutrition. They mainly ate to satisfy their hunger. The subjects' eating behavior had a significant difference according to their socio-demographic characteristics. The aged and the less educated valued breakfast the most, and showed a preference for rice as the staple food. The rate of skipping meals for this group was low. The higher their income, their eating behavior score was equally high, while the primary industrial workers and rural inhabitants had a low eating behavior score. Value toward food and nutrition had a significant influence on eating behavior. Those that placed a high value toward food and nutrition showed a marked tendency to choose bread or noodles rather than rice. In addition, those who placed a high value on food and nutrition skipped fewer meals and had a higher eating behavior score.

• 동아시아식생활학회지
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• 제23권1호
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• pp.31-38
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• 2013

본 연구는 밥 중심 식사 패턴이 비만에 미치는 영향을 조사하고자 C57BL/6 마우스를 고지방식이 대조군(HFD), 밥 중심 식사 급여군(RD) 및 빵 중심 식사 급여군(BD)으로 분류하여 각 실험 식이를 5주간 공급하여 마우스의 체중 변화, 혈중 지질 함량, 지방조직의 무게 및 혈중 glucose 및 insulin 함량을 비교 측정하였다. 실험 결과, 모든 처리군에서 사료 섭취량의 유의적인 차이를 나타내지 않았으나, RD 급여군에서의 체중 증가량은 BD 급여군에 비하여 유의적으로 낮아졌다(p<0.05). 지방조직 무게 역시 RD 급여군에서 낮은 수치를 보였으며, 특히 부고환지방의 무게 및 지방세포의 크기는 BD 급여군에 비하여 현저하게 감소하였다. RD 급여군에서의 혈중 중성지방 및 총콜레스테롤, 포도당 및 인슐린 농도 역시 BD 급여군에 비하여 유의적으로 낮았다(p<0.05). 이상의 실험 결과를 종합해 볼 때, 밥 중심의 식사는 고지방 식이를 비롯한 빵 중심 식사에 비하여 체내 지방조직의 축적을 억제하고, 혈중 지질농도를 감소시키며, 혈당의 증가를 억제함으로써 비만 및 당뇨병 등의 대사성 질환을 개선하는 데에 있어서 효과적인 것으로 사료된다.