• Journal of Life Science
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• v.27 no.6
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• pp.611-616
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• 2017

The antibacterial activities of nine oriental herbal medicine extracts were measured against seven kinds of bacteria known to cause food poisoning and disease. The extracts were prepared with distilled water using an autoclave ($121^{\circ}C$ for 30 min). The extraction yields of nine oriental herbal medicines were in the rage of 0.5%-33.4%. The antibacterial activities were evaluated using the paper-disc method. Extracts of Hwangryeon, Jiyu, and Ohbaeja showed antibacterial activities while the others did not. Extracts of Coptis japonica, Hwangryeon, and Sanguisorba officinalis, Jiyu showed antibacterial activities only against Clostridium perfringens and Listeria monocytogenes. Extracts of Rhus javanica, Ohbaeja showed the strongest antibacterial activities against all the bacteria tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of R. javanica extracts were in the range of 2.4~20 mg/ml for the bacteria tested. In conclusion, the extract of R. javanica showed superior antibacterial activities against the seven bacterial strains tested. The MIC and MBC values for each bacterium were represented as strain names (MIC, MBC), which were Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), and Vibrio parahaemolyticus (2.4, 5.3). Further purification and study of this antibacterial material would be helpful for developing antibiotics or promoting synergistic effects with known antibiotics, producing antibacterial material for lengthening the shelf-life of food, and so on.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.677-682
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• 1993

Hexane(Hx), ethyl acetate(EtoAc) methanol(MeOH) and 99% ethanol(EtOH) extract of Rhus javanicus Lines with synergists e.g. ascorbic acid(AA), citric acid(CA) and ${\delta}-tocopherol$(TO) were tested their antioxidative effect on palm oil and lard by Rancimat. The methanol showed the highest extraction yield as 14.53%(w/w). When each 600 ppm of Hx, EtOAc, MeOH and EtOH extract with 200 ppm of AA was added to palm oil, the antioxidative index(AI: induction time of oil containing of each extract/induction time of test oil) were 1.83, 2.25, 2.81 and 2.85 respectively which were higher than other treatments and 600 ppm of each extract with 200 ppm of TO to lard, the AI were 3.64, 7.83, 7.34 and 9.30 respectively. Each solvent fractionate of EtOH and EtOAc extracts resulted no higher antioxidative effect than crude whole extract. Palm oil and lard containing 600 ppm of methanol extract were very stable comparing with the control by POV and TBA at oven test($60^{\circ}C$).

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.1
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• pp.45-54
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• 2014

Lipopolysaccharide (LPS)-induced inflammatory responses in the HaCaT keratinocyte cell line produce proinflammatory cytokines, such as interleukin-1${\alpha}$(IL-1${\alpha}$), tumor neurosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-6 (IL-6), and interleukin- 8 (IL-8) and also increase the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandins E2 (PGE2). In this study, we developed new natural ingredients for cosmetics that inhibit the pro-inflammatory responses induced by LPS in HaCaT cells. The mixture of Sorbus commixta (SC), Urtica dioica (UD), Phyllostachys nigra (PN), and Rhus semialata gall (RS) extracts blocked the increase of TNF-${\alpha}$ IL-1${\alpha}$ IL-6, and IL-8. The increase of COX-2, iNOS, and PGE2 were also blocked by it. Finally, the mixture inhibited skin irritation induced by sodium lauryl sulfate (SLS), when applied on skin through IQ chamber$^{(R)}$. In conclusion, these results show that the mixture of SC, UD, PN, and RS can be used as a primary ingredient to alleviate skin irritation when cosmeceutical products are developed for sensitive skin.

• Natural Product Sciences
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• v.13 no.1
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• pp.1-5
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• 2007

Dietary flavonoids have antioxidant and antitumor promoting effects. Rhus verniciflua Stokes (RVS) is a flavonoid-rich herbal medicine and has long been used as a food additive and an antitumor agent in Korea. Previous study demonstrated that a purified flavonoid fraction prepared from RVS, herein named RCMF (the RVS chloroform-methanol fraction), exhibited growth inhibition and induced apoptosis in human osteosarcoma(HOS) cells. This study evaluated if p53-mediated pathway is associated with the RCMF-induced apoptosis in HOS cells. RCMF was shown to be capable of inducing apoptosis of the cells, as expected, and transparently increased p53 expression in the cells. However, the RCMF-induced cytotoxicity was suppressed by transfecting the cells with antisense p53 oligonucleotide, which also inhibited the decrease of Bcl-2 and the increase of Bax in mitochondria, and the release of cytochrome c into cytosol. This finding suggests that p53-mediated mitochondrial stress is required for RCMF-induced apoptosis in HOS cells.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.260-269
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• 1998

This study was conducted to screen the biological activity of urushiol in the sap of lac tree(Rhus verniciflua STOKES) which has been used in traditional folk remedies. Cytotoxic activity of urushiol was screened with L1210(mouse luekemia cell), PC-9(human lung adenocarcinoma cell), A427(human lung adenocarcinoma cell) and KATO III (human stomach adenocarcinoma cell) The stepwise hexane : acetone eluent fractions of the urushiol were obtained by the silica gel adsorption column chromatography and added to the culture media containing L1210, PC-9. A427, and KATO III, respectively. A hexane : acetone(90 : 10, v/v) eluent fraction of them showed the lowest 50% inhibition concentration($IC_{50}$) of $0.018{\mu}g/m{\ell}$ for the cell line of A427. Much lower level of $IC_{50}$ of the hexane : acetone(90 : 10, v/v) eluent fraction of the urushiol showed the equal inhibition effect with tetraplatin(i.e., anti-cancer drug of platinum complexes) on the cancer cell lines as follows ; 3.4 times lower for L1210, 3.9 times lower for PC-9, and 105.5 times lower for A427. However, $IC_{50}$ of the hexane : acetone(90 : 10 v/v) eluent fraction for KATO III was exceptionally 3.9 times higher than that of tetraplatin.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1491-1496
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• 2008

Twenty natural plants from Jeju were extracted by pressurized organic solvent (100% methanol, $40^{\circ}C$, 13.6 MPa, 10 min). Extraction yield, total phenolic content (TPC) and integral antioxidant capacity were measured, and each component was identified by GC/MS. Extraction yields were high as 21.8%, 21.5, 21.1, 20.7 and 20.1% in Rhus javanica, Euscaphis japonica, Alnus firma, Sapium japonicum and Sorbus alnifolia, respectively. The extracts containing high TPC (mg GAE/g of dry sample) were obtained from Malus sieboldii (68.3), Sapium japonicum (57.6), Pyrrosia lingua (56.6) and Euscaphis japonica (55.1). Integral antioxidant capacities of water-soluble substances were 598, 394, 293 and $270\;{\mu}mol$ ascorbic acid equivalent/g in Geranium thunbergii, Sapium japonicum, Cornus kousa and Rhus javanica, respectively. Integral antioxidant capacities of lipidsoluble substances were 611, 314, 296 and $242\;{\mu}mol$ trolox equivalent/g in Ardisia crenata, Ostrya japonica, Geranium thunbergii and Quercus acuta, respectively. Fifteen major peaks were identified by GC/MS from the extract of pressurized organic solvent from Sapium japonicum. Two polyphenols (gallic acid (retention time (RT) 19.7 min)) and quercetin (33.5 min)), ascorbic acid (RT 35.3 min), and several fatty acids (retention time 18.6, 21.0, 21.8, 21.9 and 23.6 min) were identified, and gallic acid was the major polyphenol component due to high peak area.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.4
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• pp.54-63
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• 1999

This study was conducted to suggest the ecological restoration and environmentally friendly revegetation technology for the rock-exposed cut-slopes by the Natural Topsoil Restoration Methods (NTRM) with the following restoration objectives; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of the disturbed slopes. On Nov. in 1995, the 5cm thick layer of artificial soil and 2cm thick layer of straw-mulching was attached at rock-exposed cut-slopes by NTRM without using anchor wire and anchor pin. The main results during four years surveying on the ground-coverage effect, plant growth, species diversity and importance values were summarized as follows. 1. Artificial soil attached at rock exposed cut-slopes was not eroded until recovered by tree and herbaceous vegetation in spite of not using anchor wire and anchor pin. Also it shows low soil hardiness and has almost the same amount of bacteria and fungi with in surrounding natural topsoil. 2. In 'combination for the woody vegetation', Lespedeza cyrtobotrya, Albizzia julibrissin, Rhus chinensis, Indigofera pseudo-tinctoria occupied upper layer vegetation. Since three years after seeding, Indigofera pseudo-tinctoria had overwhelmed the other woody plants and cool season foreign grasses, Erigeron canadensis, Taraxacum mongolicum, Commelina communis, Arundinella hirta (Thunberg) and Oenothera erythrosepala grows at lower part of the vegetation, 3. The heights of the Rhus chinensis grows 1.8m, Indigofera pseudo-tinctoria 2.0m, so it seems that the objectives of woody vegetation with native plants could be accomplished. 4. After 4 years later after seeding in 'combination for the herbaceous vegetation', the most dominant plant was Indigofera pseudo-tinctoria, the next was in order of cool-season grasses, Taraxacum mongolicum, Erigeron canadensis, lxeris dentata (Thunberg), Oenothera erythrosepala, Arundinella hirta (Thunberg). The diversity index in 'combination for woody vegetation' was higher than that in 'combination for the herbaceous vegetation'. The tendency of the intrusion of secondary succession plants was more effective in 'combination for the herbaceous vegetation' than in 'combination for the woody vegetation'.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• Journal of the Korean Electrochemical Society
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• v.2 no.1
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• pp.31-37
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• 1999

Copper-containig enzyme, laccase (Rhus vernicifera) was immobilized onto gold electrode using self-assembly technique and its surface properties and catalytic activities were examined. Laccase is an oxidoreductase capable to oxidize diphenols or diamines by 4-electron reduction of molecular oxygen without superoxide or peroxide intermediates. The electrode surface were modified by $\beta-mercaptopropionate$ to have a net negative charge in neutral solution and positively charged laccase (pI=9) was immobilized by electrostatic interaction. The successful immobilization was confirmed by cyclic voltammograms which showed typical surface-confined shapes and behaviors. The amount of charge to reduce the surface was similar to the charge calculated assuming the surface being covered by monolayer. The activity of the immobilized enzyme was tested by the capbility of oxidizing a substrate, ABTS (2,2-azine-bis-(3-ethylbenzthioline-6-sulfonic acid) and it was maintained for $2\~3$ days at $4^{\circ}C$. The immobilzed laccase showed about $10\~15\%$ activity compared to that in solution. The laccase-modified electrode showed the activity of elefoocatalytic reduction of oxygen in the presence of mediator, $Fe(CN)_6^{3-}$ The addtion of azide which is an inhibitor of laccase compeletly eliminated the catalytic current.

• Journal of Conservation Science
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• v.26 no.4
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• pp.371-376
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• 2010

The great jar estimated in Proto-Three Kingdoms period was found at the site from Pyeongtaek Daechuri and the bonding traces from pieces destroyed when they were excavated are observed. Therefore this study would figure out the components of bonding material used in great jars by optical microscope, FT-IR, py-GC/MS and the kind of textile attaching to great jar by transmitted light microscope. As a result of optical microscope for the cross-section, black material is uniformly applied between a piece of jar and the part of bonding and the textile line are observed. Black bonding material is resulted in korean rhus lacquer by FT-IR and py-GC/MS and the kind of textile is identified as hemp by transmitted light microscope. Materials for repairing archaeological objects are hardly known causing little information, but this study proves that the bonding material had used lacquer for great jars before Proto-Three Kingdoms period. Therefore if ancient bonding materials are systematically analysed in the further studies, it would be significant help for studying bonding and restoration materials of ancient objects.