• Journal of Environmental Health Sciences
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• v.28 no.5
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• pp.28-34
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• 2002

Antigenicity of Rhodotrula rubra isolated from pulmonary tissue of pulmonary tuberculosis patients was studied by means of agglutination reaction with R. rubra whole cell antiserum. And the serological reactivity of crude polyfac charide from R. frubra, Candida albicans, Candida tropicalis, Candida, glabrata, and Saccharomyces cerevisiae ATCC 26603 with antiserum to R. rubra whole cell was studied by means of immunodiffusion test. R. rubra showed stationary phase after 48h when it was cultured in GYEP broth. While agglutinogen titer was 1:64 at lag phase, agglutinogen titer was 1 :256 after 20h. After growth of R. rubra on different 11 media, nutritional environment showed similar agglu-tination reartivity. The agglutinogen titer of C. albicans, C. tropicalis, C. giabrata, which were isolated from patient's expectoration, to R. rubra antiserum by means of agglutination reaction were 1:16, respectively. But, Sacch. cervisiae ATCC26603 was negative. Those results were lower than that of R. rubra agglutinogen titer 1:256. As a result of immu-nodiffusion test with crude polysaccharide extracted from cell wall of R. rubra, C. albicans, C. tropicalis, C. glabrata, Sacch. cervisiae ATCC26603, precipitin line was found only with R. rubra, of which antibody titer was 8.

• Korean Journal of Food Science and Technology
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• v.12 no.2
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• pp.133-139
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• 1980

The extraction mechanism and extraction conditions of cellular lipid of freeze-dried cells of Rhodotorula rubra YUFE 1526, which was reported as coenzyme Q producing microorganism, were studied. 1. Methyl alcohol was the most appropriate solvent for extraction of cellular lipids and the resulting total lipid was 19.17 weight %. 2. When 1 % (w/v) of freeze-dried cell was extracted by methyl alcohol, the extraction yield was 90.49 % at $50^{\circ}C$ for 2 hr. 3. The diffusivity varied with extraction temperature and the empirical equation was derived as follows : $2.2e^{0-02907T}{\times}10^{-9}\;mm^2/min.$ 4. The diffusivity of cellular lipid of Rhodotorula rubra YUFE 1526 was $9.4{\times}10^{-9}\;mm^{2}/min.$ 5. The extraction model was well fitted on the extraction of cellular lipid.

• The Korean Journal of Mycology
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• v.20 no.3
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• pp.269-272
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• 1992

208 yeast strains were isolated from air at El-Minia city, Egypt. Using 22 morphologi cal and physiological merkmals, these strains were identified and assignned to eight species belonging to seven genera. Rhodotorula mucilaginosa and Cryptococcus albidus were the most dominant species followed by Debaryomyces hansenii. These yeast species occurred regularly, whereas other species appeared sporadically. Rh. rubra, Saccharomyces kluyveri and Rh. aurantiaca were also isolated from the sampled air. The results of this study showed that time, hight and area of air sampling affect composition and total counts of yeast microflora in the air.

• Journal of environmental and Sanitary engineering
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• v.4 no.1 s.6
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• pp.17-28
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• 1989

Having studied the production of monoclonal antibodies for developing a diagnosis medicine which shall be detected by a high-sensitivity test by using Rhodotorula rubra as a fungi-host which had been extracted through biochemical tests and follow-up examinations on Yeast-like fungi obtained from pulmonary tissues of pulmonary tuberculosis patients who had been in Kong ju National Tuberculosis Hospital from Jun. to Dec. in 1987, I. have gained such results as follows: 1. The fusion rate was influenced by feeder cell layers, cell density and time required to the cell fusion with cells in myelona subculture. 2. The fusion rate did not show any significant difference when the cell was applyed with two molecular weights, i.e., 1500 and 4000, of polyethylene glycol. 3. Fused cells after the addition of HAT selection media were bright and round, whereas unfused myelona cells and spleen cells were shrunk and granulated. 4. The cell fusion rate turned out to be about $57.2\%$(150 wells / 264 wells). 5. $10\%$(15 wells / 150 wells) of the positive reaction was detected in monoclonal antibody screening. 6. The titer which had reacted positively to Rhodotorula rubra fungal-host was 800 times in density after the gradual dilution of the produced monoclonal antibodies with Indirect ELISA method. 7. The Strongest specific reaction came out after the peroxidase labelled anti-human Immunogobulin had been applyed to Rhodotorula rubra for activating its nature after making drift with Carbonate-bicarbonate buffer (pH 9.6) and drying completely.

• KSBB Journal
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• v.8 no.3
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• pp.295-299
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• 1993

The effect of fungal elicitor, Pluronic F-68 and methylcellulose on suspension culture of M piperita cells was investigated in shake flasks. About a two-fold increase in oil production was observed in response to the treatment of the fungal elicitor prepared from Rhodotorula rubra. Low concentration of Pluronic F-68 or methylcellulose enhanced Peppermint cell growth at 100 rpm of agitation.

• Korean Journal of Microbiology
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• v.19 no.4
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• pp.174-178
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• 1981

Psychrophilic yeast strains, HJ3011, HJ3023, and HJ3021, which were isolated from the Han River during winter season of December 1980 to February 1981 were identified as Candida sp., Candida nivalis, and Rhodotorula rubra respectively. The effect of temperature on growth was carried out in this study. Growth rate constants of strains HJ3011 and HJ3023 were the highest value at $15^{\circ}C\;and\;16{\sim}20^{\circ}C$ respectively, and both growth rates diminished gradually above $20^{\circ}C$ and were decreased to zero at $25^{\circ}C$. According to the results obtained in this experiment, above two strains of yeast were confirmed as psychrophile. On the other hand since the strain of HJ3021 exhibited the highest growth rate constant at $15^{\circ}C$ and could slightly grow above $25^{\circ}C$, this strain was defined as psychrotroph.

• Korean journal of food and cookery science
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• v.4 no.1
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• pp.41-46
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• 1988

Omija tea with a pH range of 2.8~3.0 was stored at $25^{\circ}C$ to study its spoilage due to microbial growth. Titratable acidity was increasing during the storage period in the tea stored with the Omija fruits but not in removed of the fruits after extraction. Microbial cells began to show up earlier in the tea without the fruits than that with the fruits. Four strains of yeasts and a strain of mold were isolated from spoiled Omija tea. Morphological, cultural and physiological characteristics of yeasts were investigated and the yeasts were identified as Rhodotorula rubra, Saccharomyces kluyveri, Cryptococcus hungaricus and Candida humicola. Morphological characteristics of the isolated mold was observed and the mold was identified as Mucor circinelloides f. janssenii.