• 제목/요약/키워드: Rhodobacter

검색결과 113건 처리시간 0.03초

A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water

  • Cho, Kyoung Sook;Kim, Joong Kyun
    • Fisheries and Aquatic Sciences
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    • 제25권10호
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    • pp.525-536
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    • 2022

  • Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scale-up production because it showed the highest specific growth rate (0.20 h-1) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 1010 colony-forming units mL-1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h-1. Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4℃ was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH4+-N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH4+-N, making seed culture easier and suitable for public use.

  • https://doi.org/10.47853/FAS.2022.e47 인용 PDF KSCI

Rhodobacter sphaeroides에 의한 ${\delta}-aminolevulinic{\;}acid$생산에 있어서 glutamic acid 및 감마 유도체의 영향 (Effect of glutamic acid and its ${\gamma}-derivatives$ on the production of ${\delta}-aminolevulinic{\;}acid$ by Rhodobacter sphaeroides)

  • 최경민;임왕진;황세영
    • Applied Biological Chemistry
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    • 제36권3호
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    • pp.184-190
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    • 1993

  • Rhodobacter sphaeroides 균주가 생산하는 ${\delta}-aminolevulinic\;acid(ALA)$의 생산성에 관하여 in vivo, in vitro 상에서 기질 및 관련 화합물을 이용하여 검토하였다. $C_5\;ALA$ 생합성계에 의한 ALA yield 대비의 $C_4$ 생합성계에 의한 비율은 in vive 상에서 0.78인 반면, in vitro 상에서의 비율은 1.37이었다.$C_4\;C_5$ 각 계의 기질 첨가 배양에 의한 cell-free system의 $C_4,\;C_5$ 계의 발현도는 미첨가 배양에 의한 system과 비교하여 각각 1.35, 1.52로 증가하였으나, 증가한 계에 대한 상대계의 발현도가 억제되어, $C_4$ 와 $C_5$ 계가 각각 0.91, 0.83으로 나타났다. ${\gamma}-Glutamyl\;derivatives$의 세포내 uptake rates는 L-glutamic acid를 기준으로 비교해서 D-glutamic acid, 0.55: D-glutamine, 0.5: L-glutamine, 0.4: ${\gamma}-L-glutamyl\;ethylester$, 0.3: GSH 및 Glu-pNA, 0의 순서를 보였다. Uptake rate와 관계없이 in vivo 상에서 L-과 D-glutamine이 L-, D-glutamic acid보다 균체 외 ALA의 생산에 있어서 각각 높은 yield의 효과를 보였다.

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$Q_{10}$ 함유 광합성세균의 반응특성 (Reaction Characterization of a Photosynthetic Bacterium Containing $Q_{10}$)

  • 정수경;김중균
    • 한국해양바이오학회지
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    • 제2권3호
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    • pp.198-200
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    • 2007

  • Cultivation of a $Q_{10}$-producing photosynthetic bacterium, Rhodobacter sphaeroids, was carried out in a 1-L flask to characterize its cellular growth reaction. The result of experiment showed that dissolve oxygen in the broth was depleted within 7 h. ORP decreased with decrease of DO, and recovered somewhat with increase of pH. The growth of R. spahaeroids reached at late-log phase in 140 h of reaction. The final pH and dry-cell weight were 7.62 and 2.2 mg/mL, respectively. The $Q_{10}$ content in the final broth was 2.35 mg/g dry cell weight, which was higher than that obtained in tube culture.

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