• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.9 no.2
• /
• pp.23-32
• /
• 2006

Total suspended solids (TSS), five-day biochemical oxygen demand ($BOD_5$), total nitrogen (T-N), and total phosphorous (T-P) concentrations around and under a floating island were examined from October 2002 to September 2003. The island was installed in July 2002 on the surface of an agricultural irrigation reservoir located in the southern part of the Korean Peninsula. It was composed of six polyethylene panels. Each panel was 2 m (length) ${\times}$ 1 m (width) ${\times}$ 0.02 m (thick) and had about thirty-two holes each with a diameter of eight centimeters, through which plant roots grew down into the water. Coconut fibers of nine-centimeters in height were placed on the panel, which sustained plants rhizomes and roots. Both the fibers and the panel were raped with polyethylene wire mashes. About thirty irises (Iris pseudoacorus) were planted into the fibers of each panel. The concentrations of TSS, $BOD_5$, T-N and T-P below the island during the iris-growing season averaged 9.70, 2.59, 3.61 and 0.14 mg/L, respectively and those around it averaged 9.99, 2.83, 4.07 and 0.16 mg/L, respectively. The average concentrations of TSS, $BOD_5$, T-N and T-P below it during the iris non-growing season were 8.68, 2.37, 3.25 and 0.14 mg/L, respectively and those near it were 8.76, 2.43, 3.34 and 0.15 mg/L, respectively. At a significance level of ${\alpha}$=0.05, $BOD_5$, T-N and T-P concentrations under the island during the iris-growing season were significantly low when compared with those around it except TSS. No differences in TSS, $BOD_5$, T-N and T-P concentrations between around and near it were found at a significance level of ${\alpha}$=0.05 during the iris non-growing season. The removal of $BOD_5$, T-N, and T-P during the growing season were significantly high when compared with those during the non-growing season. TSS abatement of the floating island was very low during both the growing and non-growing seasons. The island's reductions of $BOD_5$, T-N and T-P were good during the growing season, especially T-N and T-P, which have been considered as primary pollutant sources causing the water quality degradation of reservoirs. The removal of T-N and T-P was primarily attributed to the absorption of nitrogen and phosphorous by the irises during the growing season.

• Food Science and Preservation
• /
• v.5 no.3
• /
• pp.224-230
• /
• 1998

In order to establish proper storage conditions for ginger, the quality changes of gingers stored at three different temperatures(7$^{\circ}C$, 12$^{\circ}C$ and 20$^{\circ}C$) and three different relative humidities(75%, 85% and 95%RH) were investigated during 150 days of storage. Gingers stored at 7$^{\circ}C$ and 20$^{\circ}C$ showed rapid quality deterioration due to chilling injury and spouting respectively in any range of relative humidity. Ginger stored at 7$^{\circ}C$ was staled to show symptoms of the chilling injury such as moldiness, shrivelling, discoloration etc after 30 days of storage ; then it was deteriorated after 90 days of storage. Ginger stored at 20$^{\circ}C$ was completely lost its storage-life after 30 days of storage because of high sprouting ratio. However ginger stored at 12$^{\circ}C$, 75%RH showed better quality than ones stored at both 7$^{\circ}C$ and 20$^{\circ}C$, but it was not so good as the one at 7$^{\circ}C$, 95%RH. Consequently storage conditions of 12$^{\circ}C$, 95%RH was observed the best effecive condition for keeping rhizomes in good quality from weight loss, spouting, mouldiness and so on.

• Food Science and Preservation
• /
• v.21 no.3
• /
• pp.421-426
• /
• 2014

In this study, the antioxidant and pancreatic lipase inhibitory activities of aqueous methanolic (70% methanol) extract from the roots of Anemarrhena asphodeloides were investigated. The extracts of four solvent fractions (the n-hexane layer, EtOAc layer, n-BuOH layer, and $H_2O$ layer) of the 70% methanol extract were also investigated. Furthermore, the total phenolic content was quantified using a spectrophotometric method. All the tested samples showed dose-dependent radical scavenging and pancreatic lipase inhibitory activities. In particular, the pancreatic lipase inhibitory activity of the ethyl acetate soluble portion (the EtOAc layer) from the rhizomes of the A. asphodeloides was higher than that of the other solvent-soluble portions. The antioxidant property of the extracts was evaluated using radical scavenging assays with DPPH and $ABTS^+$ radicals. 1000 mg/ml of the n-BuOH layer extract showed 91.2% DPPH radical scavenging activity. The EtOAc layer extract and the n-BuOH layer extract showed $IC_{50}=20.5{\pm}1.7mg/ml$ and $IC_{50}=50.5{\pm}0.7mg/ml$ $ABTS^+$ radical scavenging activities, respectively. The anti-obesity efficacy of the A. asphodeloides extract was tested via porcine pancreatic lipase assay. A pancreatic lipase inhibitory activity ($IC_{50}$) of $31.3{\pm}0.1mg/ml$ was obtained from the EtOAc layer extract. These results suggest that A. asphodeloides can be considered a new potential source of natural antioxidant and anti-obesity agents.

• Korean Journal of Plant Resources
• /
• v.31 no.1
• /
• pp.37-51
• /
• 2018

Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, Sinarisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.

• Korean Journal of Pharmacognosy
• /
• v.33 no.4 s.131
• /
• pp.399-403
• /
• 2002

Three melanogenesis inhibitors were isolated from the roots and rhizomes of Veratrum nigrum L. and were identified as (3S,20S,25S)-22,26-iminocholesta-5,22(N)-dien-3-ol (verazine), (3S,2OR,25S)-22,26-iminocholesta-5,22(N)-dien-3-ol (epi-verazine) and (3R,23R)-14,15,16,17- tetradehydroveratraman-3,23-diol (veratramine) on the basis of their spectroscopic data. It was turned out that these compounds did not directly inhibit tyrosinase activity, the key enzyme responsible for the formation of melanin pigment while these compounds showed strong inhibition on the melanogenesis in B16 F1 mouse melanoma $(IC_{50}<1\;{\mu}g/ml)$. Due to the strong inhibitory activity and safety compared to current whitening agents such as arbutin, kojic acid and AHA, the compound can be a good candidate for new skin whitening agents.

• Journal of Environmental Science International
• /
• v.28 no.12
• /
• pp.1061-1069
• /
• 2019

Common reed (Phragmites australis) is widespread in reclaimed land and wetland habitats. Every year, the common reed produces extensive colonies by means of underground rhizomes and ground-surface stolons. From an agricultural point of view, the common reed's large biomass is a good material for supplying organic matter. However, it has not yet been studied in terms of seedling production, transplanting conditions, and decomposition characteristics in reclaimed land. Seeds were harvested from the native common reed in Saemangeum, South Korea, the previous year and stored on an open field. The seeds were sowed in the greenhouse at the beginning of April. Common reed decomposition was studied from June to September, with the use of coarse mesh (5 mm) stem litterbags, on three samping dates and with five replicate packs per sample. These packs were dug in five soil condition (low-salinity topsoil, subsoil, high-salinity topsoil, subsoil, paddy topsoil) to 0.2 m and 0.4 m depth. The highest germination rate of common reed seeds was observed in non-salt solution, but the exhibited germination rate was 70% at 9.38 dS m^-1. The plant height of young reed decreased steadily with increasing salinity, but leaf number did not decrease by 9.38 dS m^-1. The survival rate of the two-year-old reed was 83.3%, which was 35% higher than that of the one-year reed. The transplant success rate was 0% in the no vinyl mulching in the soil, but the first year and second year seedlings survived rates were 63% and 83.3%, respectively, in vinyl mulching. Common reed decomposition rates were faster low salinity than high salinity. All nutrient contents were found to fluctuate significantly with time by soil conditions. We also need to study the growth rate of reed transplanting seedlings by soil moisture contents and the comparison of degradation in common reed tissues.

• Korean Journal of Environmental Biology
• /
• v.38 no.1
• /
• pp.197-205
• /
• 2020

With the purpose of designing companion planting of groundcover plants for ornamental uses, this study identified the yearly growth characteristics of nine species of different life forms, analyzed the coverage characteristics of individual plants, and suggested combinations of plants suitable for each life form. Polygonatum odoratum var. pluriflorum, Liriope platyphylla and Hosta capitata, as short-grained plants that can grow to more than 20 cm, tended to grow for 60 days after planting in April and maintain their shape thereafter. Their aerial parts started to wither and enter dormancy after September. Saxifraga stolonifera, Dianthus chinensis and Sedum middendorffianum tended to continuously grow until September after planting in April and their growth declined after September. Lysimachia nummularia, as a creeping plant that grows creeping on the ground, started to show a rapid growth three months after planting. Sedum sarmentosum grew slowly until August and the aerial parts started to wither from September when the temperature decreases. The coverage characteristics of these nine species that grow differently after companion planting were surveyed and the growth of Sedum sarmentosum showed the highest number of companions. It was found that Hosta capitata can be companion planted with Sedum middendorffianum, Saxifraga stolonifera, and Lysimachia nummularia. These results indicate that among different shoot growth types species propagated with their stems creeping on the ground or those that can grow vegetatively with non-rhizome parts are more suitable for companion planting with others than those of which rhizomes branch.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.33 no.2
• /
• pp.100-104
• /
• 2013

Zoysiagrass (Zoysia japonica Steud.) is an important forage and turfgrass that spreads by stolons and rhizomes. Zoysiagrass stolon can be used directly for Agrobacterium-mediated genetic transformation by exploiting the potential of direct shoot formation. However, surface sterilization of field-grown stolons is difficult and remains to be explored. We developed an effective surface sterilization and culture method using the stolon explant for infection with Agrobacterium tumefaciens. Among various treatments, sequential disinfection in 30% bleach for 15 min followed by 0.1% mercuric chloride for 25 min resulted in the highest number of clean stolons. The efficacy of mercuric chloride was increased under vacuum conditions by incubating at 800 mbar for 5 min. The inclusion of 2.5 mg/l amphotericin B further prevents fungal growth in in vitro cultures. This protocol would speed up the development of transgenic plants by utilizing field-grown stolon nodes.

• Food Science and Preservation
• /
• v.7 no.3
• /
• pp.262-266
• /
• 2000

The rhizomes of Polygonatum odoratum(Dunggulle in korean name) have been used as a traditional medicine for a tonic, remedy of fevers of influenza, cough and lung trouble. In this experiment, water extract of Dunggulle was added in brewing korean traditional rice wine. Fermentation characteristics such as content of total acid, pH, temperature, total sugar and alcohol concentration were investigated during fermentation periods. Changes of fermentation characteristics were remarkable in the initial period(0~3 days), slow in the middle(4~6 days), and almost unchangeable in the final(7~10 days). Total acid content was remarkably increased during the initial period but slowly decreased afterward. As the content of Dunggulle extract is increased, total sugar content was slightly increased. At the first day of fermentation, total sugar content was 19.6% in control, 22.6, 23.3 and 25.6% in Dunggulle extract 2.5, 5.0 and 7.5% added group, respectively. In the initial period, content of alcohol in control was highest than in Dunggulle extract added group. The content of alcohol in 4 kinds of wine showed highest value at the sixth day and those of control and Dunggulle extract 2.5% were 16.0, 16.1%, respectively. Sensory evaluation in taste was not significantly different among control and Dunggulle extract added group.

• Korean Journal of Medicinal Crop Science
• /
• v.6 no.3
• /
• pp.210-215
• /
• 1998

Solomon's seal (Polygonatum odoratum) was traditionally propagated by its rhizomes but seed propagation has not been practiced due to their dormancy and high price. To improve the seedling production through seed pretreatments, the experiment was conducted to determine the effect of seedcoat softening and seed prechilling on seed germination and bulbil formation of the seal. The prechilling was done for 4 to 16 weeks at $3^{\circ}C$ and the softening was forced with 10% KOH for 40 minutes. Seed germination, bulbil formation and length, no. of root and root length were measured at 6 and 12 weeks after sowing. Seed germination and bulbil formation were greater at 8 to 12 week prechilling than at shorter or longer treatment when only the prechilling was enforced. Prechilling had greater effect compared to seed coat softening. In addition, the softening treatment before prechilling showed higher germination and bulbil formation compared to single treatment of them. The softening treatment prior to prechilling was better than the reverse treatment in number of root except the above two characters. Duration of prechilling treatment was able to be shortened below 8 weeks when the softening treatment was applied before prechilling.