• 생명과학회지
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• 제18권6호
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• pp.759-763
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• 2008

2007년 전라남도 여수시 남면 안도리에서 발견된 국내 미기록종 해오말은 지리적으로 열대부터 아열대까지 넓게 분포하는 식물로서 열매를 만든다. 잎의 모양은 계란형에 가깝고, 입꼭지는 견고하고 잎을 지지하고 있다. 뿌리는 불규칙적으로 뻗어있고, 뿌리 사이로 꽃이 형성되어 있다. 잎맥은 잎의 가장자리와 공간을 유지하고 있다. ITS1과 ITS2 부위은 한국산과 일본산 해오말은 100% 동일한 염기서열을 나타내고 있으나, 5.8S에서 한국산 해오말은 202 bp에서 206 bp까지 4개의 염기가 삽입된 것이 보였다. ITS 부위에 대한 한국산 해오말은 일본산과 동일한 유전적 clade을 나타내었으나, 필리핀, 호주, 베트남, 말레이시아산 해오말과는 유전적 분리를 보였다. 따라서 한국산 해오말은 일본에서 gene flow로 된 것으로 추정되며, 아열대성인 해오말이 우리나라 연안에 나타난 것은 기후변동에 의한 수온상승과도 밀접한 관계가 있는 것으로 보인다.

• Biomolecules & Therapeutics
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• 제20권1호
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• pp.36-42
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• 2012

Baicalin, a main constituent of the rhizome of Scutellaria baicalensis, is metabolized to baicalein and oroxylin A in the intestine before its absorption. To understand the role of intestinal microflora in the pharmacological activities of baicalin, we investigated its anti-inflammatory effect in mice treated with and without antibiotics. Orally administered baicalin showed the anti-inflammatory effect in mice than intraperitoneally treated one, apart from intraperitoneally administered its metabolites, baicalein and oroxylin A, which potently inhibited LPS-induced inflammation. Of these metabolites, oroxylin A showed more potent anti-inflammatory effect. However, treatment with the mixture of cefadroxil, oxytetracycline and erythromycin (COE) significantly attenuated the anti-inflammatory effect of orally administered baicalin in mice. Treatment with COE also reduced intestinal bacterial fecal ${\beta}$-glucuronidase activity. The metabolic activity of human stools is significantly different between individuals, but neither between ages nor between male and female. Baicalin was metabolized to baicalein and oroxylin A, with metabolic activities of $1.427{\pm}0.818$ and $1.025{\pm}0.603$ pmol/min/mg wet weight, respectively. Baicalin and its metabolites also inhibited the expression of pro-inflammatory cytokines, TNF-${\alpha}$ and IL-$1{\beta}$, and the activation of NF-${\kappa}B$B in LPS-stimulated peritoneal macrophages. Of them, oroxylin A showed the most potent inhibition. Based on these findings, baicalin may be metabolized to baicalein and oroxylin A by intestinal microflora, which enhance its anti-inflammatory effect by inhibiting NF-${\kappa}B$ activation.

• 원예과학기술지
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• 제32권5호
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• pp.623-627
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• 2014

Curcuma alismatifolia 'Chiangmai Pink'의 화아발달 과정을 SEM을 통해 관찰하였다. 쿠르쿠마 근경에서 자란 맹아의 발육정도에 따른 화아분화 과정과 단계별 화아분화의 형태적 특성은 정식 후 식물체의 싹이 15-20cm 가량 자랐을때 생장점의 중앙부가 돔형으로 비대되는 경정분열조직에서 포엽이 분화되기 시작하였다. 포엽이 5-7매 가량 분화되면 포엽과 포엽 사이에서 화아가 형성되기 시작하였고, 보통 포엽당 화아는 4-6개가 관찰되었다. 화아가 분화되는 순서는 맨 하위 포엽 내 존재하는 것부터 상부로 올라가면서 하나씩 분화되었고 상부의 것이 분화되면 다시 하부의 4-6개의 화아 중 또 다른 화아가 상부쪽으로 향하면서 하나씩 순차적으로 되었다.

• Preventive Nutrition and Food Science
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• 제21권3호
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• pp.289-295
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• 2016

Curcumin is a flavonoid found in the rhizome of the turmeric plant (Curcuma longa L.) and has recently attracted interest because it has numerous biological functions and therapeutic properties. In the present study, we attempted to incorporate curcumin into medium-chain triglyceride (MCT) nanoemulsions (0.15 wt% curcumin, 10 wt% MCT oil, and 10 wt% emulsifiers) with various emulsifiers [polyoxyethylene (20) sorbitan monolaurate (Tween-20), sorbitan monooleate (SM), and soy lecithin (SL)]. The physicochemical properties of the nanoemulsions including the Ostwald ripening stability were investigated. The initial droplet size was found to be 89.08 nm for the nanoemulsion with 10 wt% Tween-20 (control), and when Tween-20 was partially replaced with SM and SL, the size decreased: 73.43 nm with 4 wt% SM+6 wt% Tween-20 and 67.68 nm with 4 wt% SL+6 wt% Tween-20 (prepared at 15,000 psi). When the nanoemulsions were stored for 28 days at room temperature, the droplet size increased as the storage time increased. The largest increase was observed for the control nanoemulsion, followed by the 4 wt% SL+6 wt% Tween-20 and 4 wt% SM+6 wt% Tween-20 systems. The Turbiscan dispersion stability results strongly supported the relationship between droplet size and storage time. The time-dependent increase in droplet size was attributed to the Ostwald ripening phenomenon. Thus, the Ostwald ripening stability of curcumin-loaded MCT nanoemulsions with Tween-20 was considerably improved by partially replacing the Tween-20 with SM or SL. In addition, curcumin may have acted as an Ostwald ripening inhibitor.

• 한방재활의학과학회지
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• 제23권1호
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• pp.1-13
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• 2013

Objectives : The pathophysiology of acute spinal cord injury(SCI) may be divided into primary and secondary mechanisms of injury. The secondary mechanism involves free radical formation, excitotoxicity, inflammation and apoptotic cell death, and sets in minutes after injury and lasts for weeks or months. During this phase the spinal tissue damages are aggravated. Therefore, secondary mechanisms of injury serve as a target for the development of neuroprotective drug against SCI. The present study investigated the effect of tetramethylpyrazine(TMP), an active ingredient purified from the rhizome of Ligusticum wallichii(川芎, chuanxiong), on neuronal apoptosis in spinal cord compression injury in rats. Methods : SCI was subjected to rats by a static compression method(35 g weight, 5 mins) and TMP was treated 3 times(30 mg/kg, i.p.) during 48 hours after the SCI. Results : TMP ameliorated the tissue damage in peri-lesion of SCI and reduced TUNEL-labeled cells both in gray matter and in white matter significantly. TMP also attenuated Bax-expressed motor neurons in the ventral horn and preserved Bcl-2-expressed motor neurons. Conclusions : These results indicate that TMP plays a protective role in apoptotic cell death of neurons and oligodendrocytes in spinal cord injury. Moreover, it is suggested that TMP and TMP-containing chuanxiong may potentially delay or protect the secondary spinal injury.

• 셀메드
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• 제5권3호
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• pp.15.1-15.12
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• 2015

Comparative studies have established that the North-Eastern (NE) region of India which is a part of the Eastern Himalayan region is affluent in both traditional knowledge based phytomedicine and biodiversity. About 1953 ethno-medicinal plants are detailed from the NE region of India out of which 1400 species are employed both as food and ethnopharmacological resources. Nearly 70% of species diversity has been reported from the two Indian biodiversity hotspots-The Western Ghats and the Eastern Himalayas and these hotspots are protected by tribal communities and their ancient traditional knowledge system. Paris polyphylla Smith belongs to the family Melanthiaceae and is a traditional medicinal herb which is known to cure some major ailments such as different types of Cancer, Alzheimer's disease, abnormal uterine bleeding, leishmaniasis etc. The major phytoconstituents are dioscin, polyphyllin D, and balanitin 7. Phylogeny of Paris was inferred from nuclear ITS and plastid psbA-trnH and trnL-trnF DNA sequence data. Results indicated that Paris is monophyletic in all analyses. Rhizoma Paridis, which is the dried rhizome of Paris polyphylla is mainly used in Traditional Chinese Medicine and its mode of action is known for only a few cancer cell lines. The current review determines to sketch an extensive picture of the potency, diversity, distribution and efficacy of Paris polyphylla from the Eastern Himalayan region and the future validation of its phytotherapeutical and molecular attributes by recognizing the Intellectual Property Rights of the Traditional Knowledge holders.

• Journal of Ginseng Research
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• 제30권3호
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• pp.153-157
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• 2006

홍삼을 습점 또는 수침처리하여 원형을 복원한 후 경흔적 기산법과 나이테염색법을 이용하여 연근을 판별하였다. 경흔적 기산법은 뇌두를 $50^{\circ}C$에서 3일간 침지하여 뇌두의 뿌리 조직을 제거하면 줄기 조직만 남게 되어 연근판별의 정확도를 기할 수 있었다. 나이테 염색법은 홍삼 절편을 만들어 기존의 safranine에 의한 나이테 염색을 시도하였으나 나이테가 염색되지 않아 연근 판별이 불가능하였다. 한편 기계건조법은 홍삼 절편을 습점 처리하여 나이테를 건조시키던가 아니면 홍삼절편을 수침 처리하여 홍삼색소를 제거한 후 건조 사키면 나이테가 선명하게 들어나 연근판별이 가능하였다.

• Archives of Pharmacal Research
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• 제27권12호
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• pp.1220-1225
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• 2004

The overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) is known to be responsible for vasodilation and hypotension observed in septic shock and inflammation. Inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by overproduction of NO. In the course of screening oriental anti-inflammatory herbs for the inhibitory activity of NO synthesis, a crude methanolic extract of Curcuma zedoaria exhibited significant activity. The activity-guided fractionation and repetitive chromatographic procedures with the EtOAc soluble fraction allowed us to isolate three active compounds. They were identified as 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one (1), procurcumenol (2) and epiprocurcumenol (3) by spectral data analyses. Their concentrations for the 50% inhibition of NO production $(IC_{50})$ in lipopolysaccharide (LPS)-activated macrophages were 8, 75, 77 ${\mu}M$, respectively. Compound 1 showed the most potent inhibitory activity for NO production in LPS-activated macrophages, while the epimeric isomers, compound 2 and 3 showed weak and similar potency. Inhibition of NO synthesis by compound 1 was very weak when activated macrophages were treated with 1 after iNOS induction. In the immunoblot analysis, compound 1 suppressed the expression of iNOS in a dose-dependent manner. In summary, 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one from Curcuma zedoaria inhibited NO production in LPS-activated macrophages through suppression of iNOS expression. These results imply that the traditional use of C. zedoaria rhizome as anti-inflammatory drug may be explained at least in part, by inhibition of NO production.

• 식물분류학회지
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• 제47권4호
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• pp.316-321
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• 2017

한국에 백록고사리[Tectaria fuscipes (Wall. ex Bedd.) C. Chr.]를 처음으로 보고한다. 이 종은 한국에서 보고된 바 없는 미늘창고사리과(Tectariaceae Panigrahi)의 미늘창고사리속(Tectaria Cav.)에 속한다. 백록고사리는 거의 직립하거나 비스듬히 올라가는 지하경, 이형엽 또는 반이형협, 유리맥이 있는 특징이 있다. 이종은 제주도 서귀포시 한라산 남서부 사면에 분포하고 있으며, 중국의 남부지역과 대만에서도 자생한다. 일본에는 미늘창고사리속 식물 8종이 보고되어 있으나 백록고사리는 아직 보고되지 않았다. 제주도는 지금까지 알려진 백록고사리의 분포 지역 중 가장 최북동 지역이며, 그 서식처는 작은 수직동굴 내부로 한정되어 있다.

• Journal of Ginseng Research
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• 제44권4호
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• pp.552-562
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• 2020

Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides are affected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer (P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem, and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterized by Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13 ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified by matching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginseng varied, which was not obviously dependent on age. In the underground parts, the 13-year-old ginseng root contained more abundant ginsenosides among tested ginseng samples, whereas in the aboveground parts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount of ginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts of P. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulated P. ginseng.