• 한국식품영양학회지
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• 제22권2호
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• pp.302-307
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• 2009

The present study was designed to clarify the antidiabetic activity and mechanism of Dioscorea rhizome in diabetic db/db mice. Mice were administered Dioscorea rhizome and rosiglitazone orally for 7 weeks and the effects of these compounds on fasting blood glucose, glucose tolerance and intestinal disaccharidase activity in db/db mice were evaluated. The fasting serum glucose of the D. rhizome treated group was reduced when compared with that of the db/db control group. In addition, the disaccharidase activities in homogenates of the proximal, middle and distal segment of the small intestine were significantly decreased response to D. rhizome treatment, especially in the middle segment. These results suggest that D. rhizome decreases blood glucose via a decrease in the activity of disaccharidase in the mucosa of the middle region of the small intestine in db/db mice.

• 한국약용작물학회지
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• 제11권3호
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• pp.186-189
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• 2003

고추냉이 시기별 생육특성을 검정하기 위하여 정식 후 10개월과 15개월에서 수확하여 특성을 조사한 결과 10개월에 비하여 15개월에서 지상부 생육 중 엽장, 엽폭, 주경엽수 등은 큰 증가를 보였으나, 총엽수, 얼자수 등은 감소하였다. 근경의 건물 분배율은 정식 10개월에서는 엽병 > 엽> 근경 > 분지경 순서이었으나, 15개월에서는 엽병 > 근경 > 엽 > 분지경 순서였다. 근경의 경도 및 allylisothiocyanate함량은 10개월에 비하여 15개월에서 13배 정도 높았다. 근경의 건물율은 10개월에서는 두부와 미부간에 큰 차이가 있었으나 15개월에서는 차이가 없었다.

• 한국약용작물학회지
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• 제12권6호
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• pp.473-477
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• 2004

고추냉이 밭재배에서 강판에 갈아 생식할 수 있는 근경을 생산하는 작부체계를 개발하기 위해 중산간부 지역인 전북 진안군에서 12개월과 7개월간 묘를 육묘하였다. 육묘된 묘는 평야부인 전북 익산지역에 9월 하순에 이식되어 이듬해 5월에 근경수량을 조사하였다. 중산간부에서 7개월간 육묘한 묘에서 근경장 5cm 이상, 근경중 40 g 이상의 상품성 있는 근경이 13.0%의 비율로 생산되었고 72.9 kg/10a의 수량성을 보였으며 근경의 allylNCS 함량도 0.7771 mg/g으로 18개월간 물재배된 근경과 대등한 함량을 보였다.

• Applied Biological Chemistry
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• 제39권4호
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• pp.260-264
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• 1996

Cymbidium속 난의 형질전환계를 확립하기 위해서, microprojectile bombardment 방법을 이용하여 춘란(Cymbidium virescence)의 rhizome 조직세포에 외래 유전자를 도입하는 조건을 검토하였다. 각 parameter별 적정조건으로서 텅스텐 입자의 크기는 $1.11\;{\mu}m$, He 가스 압력은 $77.33kg/cm^2$, gap distance는 6.35mm, target distance는 7.0cm 이었다. DNA피복입자를 투사한 $400{\mu}m$ 두께의 rhizome 절편을 2개월간 배양한 뒤 GUS 활성을 조사한 결과 이 유전자가 발현되는 세포들이 관찰되었다. Kanamycin (100 mg/L)을 첨가한 배지에서 6개월 동안 선택배양을 통해 얻은 rhizome의 DNA를 PCR로 분석한 결과 nptII 유전자의 삽입을 확인할 수 있었다.

• Journal of Ginseng Research
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• 제43권3호
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• pp.385-393
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• 2019

Background: The root and rhizome are historically and officially utilized medicinal parts of Panax notoginseng (PN) (Burk.) F. H. Chen, which in raw and steamed forms are used differently in practice. Methods: To investigate the differences in chemical composition and bioactivities of PN root and rhizome between raw and steamed forms, high-performance liquid chromatography analyses and pharmacologic effects evaluated by tests of anticoagulation, antioxidation, hemostasis, antiinflammation, and hematopoiesis were combined. Results: With the duration of steaming time, the contents of ginsenosides $Rg_1$, Re, $Rb_1$, Rd, and notoginsenoside $R_1$ in PN were decreased, while those of ginsenosides $Rh_1$, $20(S)-Rg_3$, $20(R)-Rg_3$, $Rh_4$, and $Rk_3$ were increased gradually. Raw PN samples steamed for 6 h at $120^{\circ}C$ with stable levels of most constituents were used for the subsequent study of bioeffects. Raw PN showed better hemostasis, anticoagulation, and antiinflammation effects, while steamed PN exhibited stronger antioxidation and hematopoiesis activities. For different parts of PN, contents of saponins in PN rhizome were generally higher than those in the root, which could be related to the stronger bioactivities of rhizome compared with the same form of PN root. Conclusion: This study provides basic information about the chemical and bioactive comparison of PN root and rhizome in both raw and steamed forms, indicating that the change of saponins may have a key role in different properties of raw and steamed PN.

• 대한본초학회지
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• 제33권4호
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• pp.53-58
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• 2018

Objectives : Kaempferia parviflora Rhizome is black ginger indigenous to Laos and Thailand. It has been used as a folk medicine to improve blood flow and promote vitality and longevity with good health and well being. For these reasons, Kaempferia parviflora Rhizome has been focused on developing it as a food or food supplement. In addition, Kaempferia parviflora Rhizome could be under consideration of new prescription based on its characteristic compounds, polymethoxyflavonoids. However, it needs to be certified as safe before it can be used. Here, a single-oral toxicity test and safety classification was carried out to identity acute information of the toxicity of Kaempferia parviflora Rhizome powder and to make sure of its safety in clinical applications. Methods : Test substance was orally administered to male and female SD-rat at dose levels of 5000 mg/kg to estimate approximate lethal dose(ALD). Based on the acute information of the toxicity, the safety classification was estimated using the HED(human equivalent dose)-based MOS(margin of safety). Results : At 14 days after treatment with test substance. there were no of test substance related with mortalities and clinical signs. In addition, no changes in the body or organ weights and no gross or histopathological findings were observed. Thus, the ALD of Kaempferia parviflora Rhizome powder was considered over 5,000 mg/kg in both female and male mice. Conclusions : Based on the single oral toxicity test using the highest and limit dose, 5,000 mg/kg and the decision guideline for safety classification based on HED-based MOS, it was estimated that Kaempferia parviflora Rhizome powder is classified as "Specified class B" indicating that clinical dose is not limited to patients as safe as food.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.15-22
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• 2023

The aim of the present study was to evaluate the chemical composition and antioxidative activity of rhizome essential oil of Kaempferia parviflora Wall. ex Baker. The essential oil extracted by hydrodistillation was chemically profiled by GC/MS analysis. The antioxidative activity was determined and evaluated spectroscopically by the 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays. According to the results, the major essential oil components were camphene (18.03%), β-pinene (14.25%), a-pinene (12.38%), endo-borneol (10.23%), β-copaene (8.38%), and linalool (8.20%). K. parviflora rhizome oil possessed antioxidant potential, exhibiting DPPH and ABTS radical scavenging activities as high as 80.90 and 94.04%, respectively, at a concentration of 10 mg/mL. The corresponding IC₅₀ values were 0.451±0.051 and 0.527±0.022 mg/mL, respectively (IC₅₀ values for ascorbic acid, as the standard, were 0.209±0.016 and 0.245±0.022 mg/mL, respectively). The mycelium of F. oxysporum was distorted and collapsed when treated with 0.5 mg/mL of the EO of K. parviflora rhizome for 3 days treatment, which may provide an important information for exploring the metabolism of the fungicide K. parviflora rhizome and its derived compounds against F. oxysporum. This study provides the chemical properties of the essential oil of K. parviflora rhizome grown in Vietnam and their potential antioxidant and antifungal activities.

• The Korean Journal of Ecology
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• 제21권5_3호
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• pp.649-663
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• 1998

The clarify the ecological properties, and to predict change of understory vegetation of mt. Nam Park, population dynamics and interspecific competition of D. smilacinum and D. viridescens, which grow in understory of deciduous broad-leaved forest and pseudo-annuals, were studied from May 20 to May 30 1998. The depth of litter layer, soil moisture content, soil organic matter and soil texture were surveyed in 18 populations (15 D. smilacinum populations and 3 D. viridescens populations). Mean litter layer of d. smilacimum population was thinner than that of D. viridescens populations). Mean litter layer of D. smilacnum population was thinner than that of D. viridescens population. The contents of soil moisture and organic matter of D. smilacinum population were lower than that of D. viridescens population. The D. smilacinum growed in broad range of soil texture but D. viridescens in loamy soil. Because D. smilacinum could tolerate more broad range of soil moisture and soil texture than D. viridescens, the former covered the herb layer in earlier stage and the latter introduced in later stage when rhizome could grow easily. The numbers of individual in two marginal parts were smaller than that in center in same D. smilacinum patch. And the total numbers of individuals grown in (10 ${\times}$ 10)cm were from 0 to 12. The rhizome (subterranean runner) weight, rhizome length, root weight, shoot weight, lea weight and leaf number per subquadrat (cell) increased along the number of individual, that is, increased from marginal part to center. But rhizome weight and rhizome length per individual were vice versa. Therefore, the individuals in marginal part reproduced longer and stronger asexual propagules than that in center. The distribution pattern of D. smilacinum was contageous and that of D. viridescens was random or regular. Therefore, population growth of former was independent on density and that of latter was dependent on density. The distributions of size-class showed normal curves in two population, but the curves based on data of total dry weight showed positive skewness and those of leaf number showed negative skewness The correlation coefficient (CC) values between the properties of each organ were high in two population and significant at 0.1% level. The CC values of D. viridescens were higher of the two. Therefore, the former allocated the energy to each organ stable. The rhizome depth of d. viridescens was 2 times deeper than that of D. smilacinum. And rhizome length and weight of D. viridescens were longer (2 times) or heavier (4 times) than those of D. smilacinum. The patch size of D. viridescens increased 60 cm per year and that of D. smilacinum 30 cm. On this results, the intrinsic increase velocity of d. viridescens patch was 2 times faster than that of d. smilacinum, therefore, on the competition, the former had an advantage over D. smilacinum. The reason why d. viridescens defeated D. smilacinum resulted from that the leaf area of former was 4 times broader than that of latter. in Mt. Nam Park, it was thought that two disporum Population would change with the 3 thpes of environmental change as followings. First, no human impact and increase of soil moisture content resulted in increase of D. viridescens population. Second, mild human impact and similar condition of soil moisture content resulted in slow increase or no changes of D. smilacinum and d. viridescens population. Third, severe human impact and dry condition resulted in decrease or vanishment of two disporum populations.

• 한국약용작물학회지
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• 제21권5호
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• pp.334-341
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• 2013

Studies were carried to evaluated the influence of storage method by temperatures and fillers on yield and quality of seed rhizome in turmeric. Seed rhizome was stored at styrofoam box filled with rice hull and sand (3:1) or vermiculite for 30, 60 and 90 days at different temperatures (5, 10 and $15^{\circ}C$. compared to traditional method (rhizome only). Parameters were obtained for weight loss, cold injury, percentage of decayed in stored rhizome during storage periods. Also, the germination, growth pattern and yield from stored rhizome has been investigated. It was confirmed that storage of turmeric in stored with filled with vermiculite helps in prevention of rhizomes from microbial and fungal attack. The storage of rhizomes in styrofoam box without any filler at low temperature below $10^{\circ}C$. is not advocated due to heavy losses weight and decayed in management of postharvest for turmeric rhizome. Germination percentage, growth pattern and yield was maximum for rhizomes stored at styrofoam box filled with vermiculite for 90 days at $15^{\circ}C$. The paper outlines a brief attempt to assess the efficacy of non-chemical methods including optimal storage method (temperature and filler) of control of decay and moisture losses during storage of turmeric.

• 생물환경조절학회지
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• 제32권3호
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• pp.210-216
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• 2023

일반적으로 지황[Rehmannia glutinosa (Gaertn.) Libosch ex Steud.]은 플러그 묘를 사용하지 않고 노지에 파종한다. 플러그 묘는 발아 촉진과 이식이 편리한 장점이 있다. 그러나 지황의 플러그 육묘를 이용한 번식에 관한 연구는 미흡한 실정이다. 본 연구는 공정육묘 방법 확립을 위한 지황의 최적 종근 길이, 굵기 및 파종 방향을 조사하기 위해 수행되었다. 종근의 길이는 1, 2, 3cm로 구분하였고, 굵기는 각각 0.3-0.5, 0.6-1.0, 1.1-1.5cm로 구분하였다. 그리고 종근은 수직 및 수평 방향으로 파종하였다. 종근 길이 1cm 처리에서 생존율이 유의적으로 낮았다. 엽장, 엽폭, 엽수, SPAD, 엽면적, 지상부와 지하부의 생체중 및 건물중은 3cm 길이에서 가장 높았다. 지황의 종근 굵기가 감소할수록 묘의 생육이 증가하는 경향을 보였다. 지황의 종근을 수평 방향으로 파종하였을 때 엽장, 엽폭, 엽수, 엽면적은 수직 방향보다 유의하게 높았다. 결론적으로 지황 종근을 플러그 트레이 파종할때, 길이 3cm로 절단한 뒤 굵기 1.0cm 이하의 종근을 수평 방향으로 파종하는 것이 적절한 방법으로 판단된다.