• IMMUNE NETWORK
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• v.8 no.1
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• pp.29-37
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• 2008

Interleukin-23 (IL-23) is a novel pro-inflammatory cytokine which has been implicated to play a pathogenic role in rheumatoid arthritis (RA). This study was undertaken to investigate the IL-23 inductive activity of the proinflammatory cytokine IL-17, $IL-1{\beta}$ and tumor necrosis factor (TNF-${\alpha}$) in RA synovial fluid mononuclear cells (SFMC). Expression of IL-23p19, IL-17, $IL-1{\beta}$ and TNF-${\alpha}$ in joint was examined by immunohistochemistry (IHC) of patients with RA and osteoarthritis (OA). The effects of IL-17 and $IL-1{\beta}$ on expression of IL-23p19 in human SFMC from RA patients were determined by reverse transcriptase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). IL-23p19 was expressed in the RA fibroblast like synoviocyte (FLS), but not from OA FLS. Similar to the protein expression, IL-23p19 mRNA could be detected by RT-PCR in RA SFMC. IL-17 and $IL-1{\beta}$ could induce RA SFMC to produce the IL-23p19. The effects of IL-17 were much stronger than $IL-1{\beta}$ or TNF-${\alpha}$. These responses were observed in a doseresponsive manner. In addition, IL-17 or $IL-1{\beta}$ neutralizing antibody down-regulated the expression of IL-23p19 induced by LPS in RA-SFMC. Our results demonstrate that IL-23p19 is overexpressed in RA synovium and IL-17 and $IL-1{\beta}$ appears to upregulate the expression of IL-23p19 in RA-SFMC.

• IMMUNE NETWORK
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• v.22 no.4
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• pp.34.1-34.19
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• 2022

Osteoarthritis (OA) is the most common form of arthritis associated with ageing. Vitamin D has diverse biological effect on bone and cartilage, and observational studies have suggested it potential benefit in OA progression and inflammation process. However, the effect of vitamin D on OA is still contradictory. Here, we investigated the therapeutic potential of vitamin D in OA. Six-week-old male Wistar rats were injected with monosodium iodoacetate (MIA) to induce OA. Pain severity, cartilage destruction, and inflammation were measured in MIA-induced OA rats. Autophagy activity and mitochondrial function were also measured. Vitamin-D (1,25(OH)₂D3) and celecoxib were used to treat MIA-induced OA rats and OA chondrocytes. Oral supplementation of vitamin D resulted in significant attenuations in OA pain, inflammation, and cartilage destruction. Interestingly, the expressions of MMP-13, IL-1β, and MCP-1 in synovial tissues were remarkably attenuated by vitamin D treatment, suggesting its potential to attenuate synovitis in OA. Vitamin D treatment in OA chondrocytes resulted in autophagy induction in human OA chondrocytes and increased expression of TFEB, but not LC3B, caspase-1 and -3, in inflamed synovium. Vitamin D and celecoxib showed a synergistic effect on antinociceptive and chondroprotective properties in vivo. Vitamin D showed the chondroprotective and antinociceptive property in OA rats. Autophagy induction by vitamin D treatment may be a promising treatment strategy in OA patients especially presenting vitamin D deficiency. Autophagy promoting strategy may attenuate OA progression through protecting cells from damage and inflammatory cell death.

• IMMUNE NETWORK
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• v.3 no.3
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• pp.248-254
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• 2003

Oral administration of antigen has long been used in the induction of immune tolerance in various animal models of autoimmune diseases including rheumatoid arthritis (RA). Alleveation of arthritogenic symptoms has been reported from RA patients who received oral administration of type II collagen (CII) without side effects, however its rather inconsistent therapeutic efficacy and variation among patients calls for more detailed investigation on the mechanism of oral tolerance to be settled as regular treatment for RA. In an attempt to understand the immunogenic processes underpinning tolerance induction by orally administered CII, we analyzed changes in the expression of costimulatory molecules and STAT/SOCS signaling messengers in the mouse model of collagen induced arthritis (CIA). We found thatin the spleen of CIA mice, that has been undergone repeated oral feeding of CII prior to the induction of arthritis, showed increased promortion of CTLA4 expressing lymphocytes than in the spleen of PBS fed control. On the other hand, cells expressing CD28 or ICOS were decreased in the spleen of tolerized mice. Tolerance induction by oral CII administration also enhanced the expression of STAT6 in both RNA and protein level, while not affecting the expression of STAT3. The expression of SOCS3, which hasbeen known to transmit STAT-mediated signals from Th2 type cytokines, remained unchanged in the spleen of tolerized mice. Interestingly transcript of SOCS1, which has been associated with Th1 related pathways, was only visible in the spleen of tolerized but not of control mice, suggesting that as in the case of IL-6 signaling, it may exert a feed back inhibition toward the Th1 type stimulation.

• The Journal of Korean Physical Therapy
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• v.2 no.1
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• pp.9-20
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• 1990

This study researched 1,408 physical therapy patients in hospital. orthopedic clinic. neurosurgical clinic, general surgical clinic, and general clinic located Taegu city on may 9. 1990. Also this study reviewed front doctor's prescripted progress note. The results obtained were as followed. 1) 4th decade of age $(22\%)$ are the highest, and 3rd, 2nd, 5th decade were followed. Many dorsopathies are not correlated with age, also they involved 2nd decade age and 3rd decade are sprain and strains of joints and adjacent muscle. 4th and 5th decade of age are rheumatism. 2) According to medical facility. They treated in hospital are other disorders of central nervous system. orthopedics are rheumatism, and in neurosurgical clinic. general surgical clinic, general clinic are dorsopathies. 3) Male and female both have a lot of dorsopathies, sprains and strains of joint and adjacents muscle occurred in males, female have a lot of rheumatism. 4 At physical therapy patients, $21.4\%$ patients are in patients and others are out patients. Among the $66.1\%$ of on patients, they treated in hospital as they have other disorders of central nervous system.

• IMMUNE NETWORK
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• v.21 no.6
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• pp.43.1-43.14
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• 2021

Group 3 innate lymphoid cells (ILC3), which express IL-22 and IL-17A, has been introduced as one of pathologic cells in axial spondyloarthritis (axSpA). Dyslipidaemia should be managed in axSpA patients to reduce cardiovascular disease, and dyslipidaemia promotes inflammation. This study aimed to reveal the role of circulating ILC3 in axSpA and the impact of dyslipidaemia on axSpA pathogenesis. AxSpA patients with or without dyslipidaemia and healthy control were recruited. Peripheral blood samples were collected, and flow cytometry analysis of circulating ILC3 and CD4⁺ T cells was performed. The correlation between Ankylosing Spondylitis Disease Activity Score (ASDAS)-C-reactive protein (CRP) and circulating immune cells was evaluated. The effect of oxidized low-density lipoprotein cholesterol (oxLDL-C) on immune cell differentiation was confirmed. AxSpA human monocytes were cultured with with oxLDL-C, IL-22, or oxLDL-C plus IL-22 to evaluate osteoclastogenesis using tartrate-resistant acid phosphatase (TRAP) staining and real-time quantitative PCR of osteoclast-related gene expression. Total of 34 axSpA patients (13 with dyslipidaemia and 21 without) were included in the analysis. Circulating IL-22⁺ ILC3 and Th17 were significantly elevated in axSpA patients with dyslipidaemia (p=0.001 and p=0.034, respectively), and circulating IL-22⁺ ILC3 significantly correlated with ASDAS-CRP (Rho=0.4198 and p=0.0367). Stimulation with oxLDL-C significantly increased IL-22⁺ ILC3, NKp44^- ILC3, and Th17 cells, and these were reversed by CD36 blocking agent. IL-22 and oxLDL-C increased TRAP⁺ cells and osteoclast-related gene expression. This study suggested potential role of circulating IL-22⁺ ILC3 as biomarker in axSpA. Furthermore, dyslipidaemia augmented IL-22⁺ ILC3 differentiation, and oxLDL-C and IL-22 markedly increased osteoclastogenesis of axSpA.

• IMMUNE NETWORK
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• v.20 no.5
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• pp.40.1-40.15
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• 2020

The protein encoded by the Gene Associated with Retinoid-Interferon-Induced Mortality-19 (GRIM-19) is located in the mitochondrial inner membrane and is homologous to the NADH dehydrogenase 1-alpha subcomplex subunit 13 of the electron transport chain. Multiple sclerosis (MS) is a demyelinating disease that damages the brain and spinal cord. Although both the cause and mechanism of MS progression remain unclear, it is accepted that an immune disorder is involved. We explored whether GRIM-19 ameliorated MS by increasing the levels of inflammatory cytokines and immune cells; we used a mouse model of experimental autoimmune encephalomyelitis (EAE) to this end. Six-to-eight-week-old male C57BL/6, IFNγ-knockout (KO), and GRIM-19 transgenic mice were used; EAE was induced in all strains. A GRIM-19 overexpression vector (GRIM19 OVN) was electrophoretically injected intravenously. The levels of Th1 and Th17 cells were measured via flow cytometry, immunofluorescence, and immunohistochemical analysis. IL-17A and IFNγ expression levels were assessed via ELISA and quantitative PCR. IL-17A expression decreased and IFNγ expression increased in EAE mice that received injections of the GRIM-19 OVN. GRIM19 transgenic mice expressed more IFNγ than did wild-type mice; this inhibited EAE development. However, the effect of GRIM-19 overexpression on the EAE of IFNγ-KO mice did not differ from that of the empty vector. GRIM-19 expression was therapeutic for EAE mice, elevating the IFNγ level. GRIM-19 regulated the Th17/Treg cell balance.

• CELLMED
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• v.4 no.3
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• pp.16.1-16.8
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• 2014

The oil palm (Elaeis guineensis Jacq.) has been reported to originate along the gulf of the guinea in West Africa. The various parts of the tree have been used locally and traditionally for various medicinal purposes. Some of these uses have been proved by scientific experiments. Palm oil is extracted from the mesocarp of the fruit and is used traditionally for the treatment of headaches, pains, rheumatism, cardiovascular diseases, arterial thrombosis and an atherosclerosis due to its rich phytonutrients. The leaves are also used for the treatment of cancer, cardiovascular diseases, kidney diseases and wound healing. The sap also has been found to be rich in phytonutrients that can be used to treat various diseases. This review therefore seeks to explore many of the uses of the oil palm using the various parts of the oil palm.

• Journal of muscle and joint health
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• v.12 no.1
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• pp.28-38
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• 2005

Purpose: This study was to understand the level of family support, self-efficacy and compliance and to examine the relationships of the above three variables in chronic arthritis. Method: The subjects of this study were 116 patients sampled among the limited population of outpatients and inpatients at Rheumatism Center of the H university hospital in S city. The research instruments used in this study were family support, self efficacy and compliance scale. In data analysis, SPSS 8.0 program was used. Result: 1. Family support and self-efficacy level were above the medium. 2. Compliance level was medificant. Conclusion: Rheumatism patients will be able to live satisfactorily if they gium. 3. The relation among the family support, self efficacy and compliance was signot a good education about efficient training program aimed at compliance.

• Journal of Rheumatic Diseases
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• v.26 no.4
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• pp.219-220
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• 2019

• Journal of the Korea Computer Industry Society
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• v.8 no.1
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• pp.23-28
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• 2007

There are many modern's overwork. excessive drinking, carriage that give stress in muscle by smoking. Because time that sit long by carriage is much. if myalgia beats widower on muscle rheumatism or union formation inflammation, core ache, there is congealment and is painful. Muscle itself is no military register change and rheumatism change shows in areolation of muscle film or muscle nerve sheath etc. that union tissue's inflammation case is around the muscle. It is typical rain joint sex rheumatism. Can assume in case of made work that do not get used to extreme exercise or body perforce. Special quality of do waveform and supersonic waves stimulation processing plant which frequency that is established in research that do Iroin is continued without change is available embodiment according to patient's state variously by series mode through mode frequency waveform, 10 modes, 25 modes, 50 modes etc. Sample that can decide basis standard for supersonic waves through an experiment, low frequency treatment equipment standard for low frequency equipment. supersonic waves treatment equipment that float in city adjusting establishment naturally establish.