• Molecules and Cells
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• v.24 no.1
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• pp.37-44
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• 2007

Three catalase cDNA clones were isolated from the small radish (Raphanus sativus L.). Their nucleotide and deduced amino acid sequences showed the greatest homology to those of Arabidopsis. Genomic Southern blot analysis, using RsCat1 cDNA as a probe, showed that catalases are encoded by small multigene family in the small radish. Nondenaturing polyacrylamide gels revealed the presence of several catalase isozymes, the levels of which varied among the organs examined. The isozyme activities were assigned the individual catalase genes by Northern analysis using total RNA from different organs. The three catalase genes were differentially expressed in response to treatments such as white light, xenobiotics, osmoticum, and UV. Their expression in seedlings was controlled by the circadian clock under a light/dark cycle and/or in constant light. Interestingly, RsCat1 transcripts peaked in the morning, while those of RsCat2 and RsCat3 peaked in the early evening. Our results suggest that the RsCat enzymes are involved in defense against the oxidative stress induced by environmental changes.

• The Korean Journal of Food And Nutrition
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• v.14 no.1
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• pp.20-27
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• 2001

The consumption of radish ( Rhaphanus sativus L.) sprout, which is Cruciferae family, is increasing because of its pungent flavor and taste. Its volatile components were analyzed by SDE (simultaneous steam distillation & extraction) method and P&T(purge & cryogenic trapping) method. As a solvent, diethyl ether and diethyl ether : pentane mixture(2:1, v/v) were used in SDE method, and diethyl ether in P&T method. Analyzing by GC and GC-MS, the major component was sulfur compounds (19 species, peak area 76.6%) with diethyl ether, sulfur compounds(15. 44.0%) and hydrocarbons(23, 23.8%) with diethyl ether-pentane mixture in SDE method. Also, hydrocarbons(25, 84.1% ) was major component in P& T method. The major volatile component of fresh radish sprout were n-heptane, methyl pentane and that of boiled radish sprout were 4-methylthio-3-butenyl isothiocyanate, methyl mercaptane, 2,3-dimethyl disulfide. Low molecular volatile components were detected more by P& T method, but types and relative quantities of volatile components were measured less comparing to SDE method.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.393-406
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• 2019

In August 2017, anthracnose symptoms were observed on the petioles and leaf veins of Korean radish (Rhaphanus sativus) in Hongcheon, Jeongseon, and Pyeongchang of the Gangwon province, Korea. Many grayish to dark-brown spots of 1-2 mm in diameter, appeared on the lower surface and leaf veins of the radish leaves. The spots gradually enlarged and coalesced to form dark-brown irregular lesions. Ten Colletotrichum isolates were obtained from the affected tissues of the Korean radish. Out of them, eight isolates were identified as C. higginsianum and two isolates were identified as C. truncatum based on morphological characteristics and multi-locus molecular phylogenetic analysis using the internal transcribed spacers and intervening 5.8S rDNA (ITS), partial beta-tubulin gene (TUB2), partial actin gene (ACT), and partial chitin synthase-1 gene (CHS1). The pathogenicity test was carried out on wounded and unwounded Korean radish (cv. Siraegimu and Osarimu), and Chinese cabbage (cv. Chuno and Smart) by inoculating with a spore suspension. All isolates except one C. truncatum isolate developed typical symptoms on both wounded and unwounded Korean radish. In Chinese cabbage, only the plants inoculated with C. higginsianum isolates developed symptoms regardless of the wound. This is the first report of anthracnose caused by C. truncatum on Korean radish in Korea.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.335-342
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• 2010

Sulforaphane (SFN) and total phenolics (TPC) contents and antioxidant activity (AA) were analyzed from 13 radish genotypes (Rhaphanus sativus L.), cultivated at 3 locations with different altitudes (Gangneung: asl 5 m, Jinbu: asl 550 m, and Daegwallyeong: asl 750 m). SFN varied greatly from 0.1 to $120.5{\mu}g{\cdot}g^{-1}$ in dry weight test and was significantly affected by location ($P{\leq}0.001$), genotype ($P{\leq}0.001$) and $location{\times}genotype$ interaction ($P{\leq}0.01$). Radishes, cultivated at Daegwallyeong site, showed higher SFN than those of other locations. Among different genotypes, the root of 'Black radish' and leaves of 'Purunmu' of Daegwallyeong had the highest SFN (107.8 and $120.5{\mu}g{\cdot}g^{-1}$, respectively). TPC in root was affected by genotype ($P{\leq}0.001$), and $location{\times}genotype$ interaction ($P{\leq}0.01$), but not by location. In leaves, TPC was affected by location ($P{\leq}0.01$), genotype ($P{\leq}0.001$), and $location{\times}genotype$ interaction ($P{\leq}0.001$). AA expressed as electron donating ability was significantly influenced by location, genotype and $location{\times}genotype$ interaction and correlated positively with TPC ($Pearson's$ $r$=0.897) in root. These results suggest that radish could be a good source of functional food and high altitude location such as Daegwallyeong has potential for the production of radish with high content of health promoting factors.

• Korean Journal of Food Science and Technology
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• v.10 no.2
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• pp.189-193
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• 1978

Effects of the special media on the mycelium growth in Agaricus campestris has been studied. The results are summarized as follows; 1. The mycelium growth of Agaricus campestris were considerably Stimulated on the Carrot (Dancus carota L.) basal medium which was added 4ml. of carrot extract, Cucumber(Cucumis sativus L.) basal medium added 3ml of cucumber extract, and Radish (Rhaphanus sativus L.) basal medium added 3ml. of radish extract during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts shows a lot of growth for 144 hours. The orders are as follows; Carrot basal medium(4ml/100ml)>Cucumber basal medium (3ml/100ml)>Radish basal medium (3ml/100ml)>Lettuce basal medium (2ml/100ml)>Cabbage basal medium (2ml/100ml). However, the Lettuce (Lactuca scariota L.) basal medium and the Cabbage (Brassica chinensis L.) basal medium among these five media are no significant differences.