• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.505-512
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• 2017

Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant ${\alpha}-GalCer$. As results, TgCPC1 DNA vaccine with or without adjuvant ${\alpha}-GalCer$ showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and $IFN-{\gamma}$ in the spleen compared to controls (PBS, pEGFP-C1, and ${\alpha}-GalCer$). Upon challenge infection with tachyzoites of T. gondii (RH), $pCPC1/{\alpha}-GalCer$ immunized mice showed the longest survival among all the groups. Mice vaccinated with DNA vaccine without adjuvant (pCPC1) showed better protective immunity compared to other controls (PBS, pEGFP-C1, and ${\alpha}-GalCer$). These results indicate that a DNA vaccine encoding TgCPC1 is a potential vaccine candidate against toxoplasmosis.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.931-937
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• 2006

The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

• Clinical and Experimental Reproductive Medicine
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• 제22권3호
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• pp.279-285
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• 1995

Despite the frequent incidence of embryo fragmentation in early human embryos, the reason of the embryo fragmentation has not been known yet. This study was conducted to investigate the histological difference(s) between fragmented (FR) and non-fragmented (NFR) human embryos focusing on comparison of mitochondrial distribution and protein synthesis. Multi-pronuclei zygotes (MPZ) such as three or more pronuclei containing in human in vitro fertilization and embryo transfer (IVF-ET) program were used for this study. MPZ were cultured in TCM-199 supplemented with 10% of human fetal cord serum (hFCS) in 5% $CO_2$ incubator at $37^{\circ}C$ for 24 hours. The cleaved embryos to 2-4 cells after 24 hours were grouped by their grade of fragmentation. Embryos were stained with Rhodamine123 (Rh123) and fluorescence was evaluated under the fluorescence microscope through PB 450-490 filter (Leitz). Regarding to protein synthesis during early human embryogenesis, there is no significant difference in the amount of synthetic proteins between FR and NFR embryos. Distribution of cytoplasmic organelles in embryos was evaluated by transmission electron microscope (TEM). The cytoplasmic distribution of mitochondria was different between FR and NFR embryos. The mitochondrial distribution was even in NFR, whereas severely aggregated in FR. It is not able to clarify in the present study whether this uneven mitochondrial distribution in FR embryo is the reason for embryo fragmentation or is the result from fragmentation. Physiological disparity related to the mitochondrial distribution may be one of the reasons for embryo fragmentation. Further studies should be addressed to investigate the physiological differences between FR and NFR embryos.

• 보존과학연구
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• 통권33호
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• pp.85-97
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• 2012

이 연구는 문화재 보존환경 간이 측정법의 적용 및 활용 가능성 검토를 위한 기초연구로 자외선 지시카드의 성능을 검증하고 이를 바탕으로 일부 전시조명에서의 적용성을 검토하였다. 5종의 자외선 지시카드를 대상으로 온 습도 환경 및 자외선램프에 의한 반응도를 살펴본 결과, L 카드와 K 카드는 반응도가 매우 낮아서 환경 측정용으로 부적합한 것으로 나타났으며, M 카드는 습도의 영향을 받는 것으로 조사되었다. 그러나 S 카드는 반응성이 가장 좋고 자외선 저 방사 환경에서도 민감한 색도 변화를 보였다. S 카드를 주요 전시조명으로 활용되는 광원에서 노출평가를 실시한 결과, 자외선량이 높은 할로겐램프와 형광램프에서 큰 색차 값과 육안상의 뚜렷한 색변화를 관찰할 수 있었다. 따라서 자외선 지시카드 중 S 카드가 문화재의 전시조명 환경 점검 및 관리에 있어 간편하게 활용할 수 있을 것이다.

• 한국축산식품학회지
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• 제36권5호
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• pp.626-634
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• 2016

This study was conducted to compare the physicochemical, microbiological and antioxidant activities of sun-dried venison amended with green tea powder (T1-3: 0, 0.5, and 1%) and Hanwoo beef jerky. Sliced beef and venison shank were marinated and sun-dried at 28-30℃ and 30-35% RH for 3.5 h. The venison jerky had a higher ash and protein content, and lower moisture and fat content than the control (p<0.05). T3 (venison+green tea powder 1%) showed a lower a_w than all other samples during storage for 10 and 20 d (p<0.05). Hunter’s color a* and b* values of T2 and T3 were lower than those of T1 and the control at day 0 (p<0.05). Saturated fatty acid was significantly higher in T1, while PUFA was higher in T2 and T3 (p<0.05). Overall sensory scores of venison jerky were lower than those of the control, except for T2, which had a similar color, flavor, saltiness and acceptability as the control. T2 and T3 showed a significant decrease in TPCs after storage for 20 d (p<0.05). The TBARS values of T3 jerky were lower than those of other jerky samples (p<0.05).

• 한국전기전자재료학회논문지
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• 제27권3호
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• pp.178-183
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• 2014

Recently, various type of nanomaterials such as nanorod, nanowire, nanotube and their core/shell nanostructures have attracted much attention in photocatalyst due to their unique properties. Among them, Type-II core/shell heterostructures have extensively studied because it has exhibited improved electrical and optical properties against their single-component nanostructure. Such structures are expected to offer high absorption efficiency and fast charge transport due to their stepwised energetic combination and large internal surface area. Thus, it has been considered as potential candidates for high efficient photocatalytic activity. In this work, we introduce a novel chemical conversion process to synthesize Type-II ZnO/ZnSe core/shell heterostructures. A plausible conversion mechanism to ZnO/ZnSe core/shell heterostructres was proposed based on SEM, XRD, TEM and XPS analysis. The ZnO/ZnSe heterostructures exhibited excellent photocatalytic activity toward the decomposition of RhB dye compared to the ZnO nanorod arrays due to enhanced light absorption and the type-II cascade band structure.

• 한국의류산업학회지
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• 제1권1호
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• pp.43-49
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• 1999

This study conducted 4 different kinds of underwear materials, which were A (Cotton 100%), B (Wool 100%), C (Cotton/Wool, 50/50%) and D (Acrylic/Cotton, 50/50%) and were done in a climate chamber under cold ambient $10{\pm}1^{\circ}C$, $40{\pm}5%RH$ by 6 male subjects who were in good health. Physiological parameters such as rectal and local skin temperature(forehead, forearm, hand, trunk, thigh, leg, foot, back and chest), heart rate, body weight loss, clothing microclimate, blood lactic acid concentration, and wearing sensation were measured. Started with a 15-min rest period, 15-min of exercise 1 (the condition of 4.5 mile/hr walking speed equivalent to with 8.5 Kcal energy consumption on the treadmill) period, 15-min rest period, exercise 2 (after 3minutes warming-up at 3.0. 3.7, 4.5. 5.2. 6.0, 6.7 mile/hr) until exhaustion period, and final 15-min of recovery period were performed. The results were as follows: The lowest mean skin temperature was acrylic/cotton in order of wool > cotton/wool > cotton > acrylic/cotton (F=13. 79. p<0.00l). Most of all skin temperature by parts of body had turned out in sequence of temperature wool > cotton/wool > acrylic/cotton > cotton. Fore arm part showed highest temperature about $32.43^{\circ}C$ on wool and had a tendency approximately $1.8^{\circ}C$ higher than cotton which had the lowest temperature, and had the biggest difference among garments in terms of skin temperature. The back temperature within clothing showed about $2^{\circ}C$ higher than the chest temperature within clothing. but the back humidity within clothing showed about 4~12% higher than the chest humidity within clothing. Body weight loss by each garment was this sequence; cotton > acrylic/cotton > wool > cotton/wool.

• Clinical and Experimental Reproductive Medicine
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• 제22권2호
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• pp.143-148
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• 1995

To present and assess the efficacy of combination of microsurgical epididymal sperm aspiration(MESA) and intracytoplasmic sperm injection(ICSI) for the treatment of infertility due to unreconstructable obstructive azoospermia or congenital bilateral agenesis of vas deferens (CBAVD), MESA was performed in the 45 husbands ( 16 CBAVD, 29 unreconstructable genital tract obstruction), followed by ICSI of oocytes recovered from the wives hyperstimulated by GnRH agonist in combination with hMG and FSH. Cleaving embryos were transfered to the uterine cavity or follopian tube(ZIFT) 18 or 24 hours after ICSI procedure. In 45 cycles of MESA, 492 oocyte complexes were recovered. ICSI was carried out on 355 metaphase II oocytes and 226 oocytes (63.7%) showed normal two pronucleus fertilization. After 198 embryos were transferred in 43 cycles, an average of 5 per cycle, 20 patients presented a positive HCG and intrauterine pregnancy was confirmed by US. So, the clinical ongoing pregnancy rate per transfer was 46.5%. Until now, 8 patients have given birth to 9 babies, 5 male and 4 female, including 1 twin. The babies were all healthy except 1 twin female baby. There was 1 miscarriage at 7 weeks and chromosomal study of abortus revealed as 45X, monosomy. These results suggested that it was possible to achieve high normal fertilization and pregnancy rate by ICSI using epididymal sperm.

• 한국통신학회논문지
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• 제30권4B호
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• pp.178-185
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• 2005

네트워크 단위의 이동성을 지원하는 network mobility (MEMO) 기술에서는 중첩된 네트워크 환경 (nested NEMO)에서 전달 지연 시간을 줄이는 경로를 최적화에 관현 연구가 활발히 이루어지고 있다. 현재 대표적인 경로 최적화 방안으로는 확장 헤드를 이용하여 경로 정보를 모두 기록하는 RRH (Reverse Routing Header)와 최상위 MR에서 하부에 위치한 MR의 상태 정보를 관리하는 BHT (Bi-directional tunnel between Home agent and Top level mobile route)이 제안되어 있다. 하지만 기존의 방안들은 중첩 깊이가 증가할수록 패킷 전달을 위한 오버헤드가 증가하는 문제가 발생한다. 본 논문에서는 중첩된 이동 네트워크 환경에서 지역적 정보를 이용한 경로 최적화 방안 (RIRO; Regional Information-based Route Optimization)을 제안하고자 한다. RIRO 방안에서는 모든 MR들은 자신의 하부에 위치한 MR들의 위치 정보를 관리하고 라우팅 헤더를 이용하여 패킷 전달 경로를 최적화하는 방안으로 중첩된 환경에서도 패킷이 전달을 위한 오버헤더가 증가하지 않는 장점이 있다.

• 한국건축시공학회지
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• 제19권5호
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• pp.421-429
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• 2019

양생조건이 GGBFS를 사용한 시멘트 콘크리트의 염화물이온 확산계수에 미치는 영향에 대한 연구를 진행하였다. 양생조건은 기중양생 ($20{\pm}2^{\circ}C$, RH $60{\pm}5%$)과 수중양생 ($20{\pm}2^{\circ}C$)으로 구분하였으며, GGBFS 치환율은 0%(대조군), 30%, 60%로 구분하여 3가지 배합의 콘크리트를 W/B 40%, 50%, 60%로 구분하여 제작하였다. 시험은 콘크리트 압축강도평가, 염화물이온확산계수 평가를 실시하였다. 콘크리트의 압축강도는 GGBFS 치환율이 증가할수록 수중양생 대비 기중양생 시험체의 압축강도의 발현율이 감소하였다. 염화물이온확산계수 측정결과 GGBFS치환율이 증가할수록 염화물이온 확산계수가 감소하였지만, 수중양생대비 기중양생 시험체의 콘크리트 염화물이온 확산계수는 증가하여 최대 111%까지 증가하는 것을 확인하였다.