• International Journal of Oral Biology
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• v.43 no.2
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• pp.53-59
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• 2018

In order to understand biological phenomena accurately, single molecule techniques using a physical research approach to molecular interactions have been developed, and are now widely being used to study complex biological processes. In this review, we discuss some of the single molecule methods which are composed of two major parts: single molecule spectroscopy and manipulation. In particular, we explain how these techniques work and introduce the current research which uses them. Finally, we present the oral biology research using the single molecule methods.

• Plant Biotechnology Reports
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• v.2 no.2
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• pp.93-112
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• 2008

Medicinal and aromatic plants (MAPs) are important sources for plant secondary metabolites, which are important for human healthcare. Improvement of the yield and quality of these natural plant products through conventional breeding is still a challenge. However, recent advances in plant genomics research has generated knowledge leading to a better understanding of the complex genetics and biochemistry involved in biosynthesis of these plant secondary metabolites. This genomics research also concerned identification and isolation of genes involved in different steps of a number of metabolic pathways. Progress has also been made in the development of functional genomics resources (EST databases and micro-arrays) in several medicinal plant species, which offer new opportunities for improvement of genotypes using perfect markers or genetic transformation. This review article presents an overview of the recent developments and future possibilities in genetics and genomics of MAP species including use of transgenic approach for their improvement.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.28 no.2
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• pp.138-140
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• 2017

Pharyngeal and laryngeal foreign bodies are common problem at ENT clinic. Removal of a foreign body is mostly simple with the manipulation of endoscope and various instruments. Rarely, migration of swallowed sharp foreign body makes complication. We describe a 74-year-old patient diagnosed with foreign body in larynx involving thyroid. In this case, we present this disease and the treatment course which has been successfully treated by surgical removal and conservative treatment. Furthermore, we discuss its symptoms, physical examinations through literature review.

• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.51-51
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• 2018

The application of recombinant DNA technology has been remarkable and nearly replaced commonly used traditional methods. Traditional industrial microbiology long depended on the discovery of valuable strains and mutagenesis of such strains to improve its secretion capacity of enzymes and secondary metabolites on the industrial scale. Commodities included industrial enzymes and biopharmaceuticals. The purpose of genome manipulation by the crossing of different strains or genetic recombination of naked DNA to the genome is of increased production of valuable metabolites. We optimized a transformation method to either for removal of innate genes, introduction of heterologous genes, or combination of both. We have been used selected whole or partial genes to manipulate target fungi toward the development of strains overproducing invaluable proteins. We have also used the whole genome sequence information of fungal genomes in public databases and functional genomics approach to select genes to manipulate and eventually contributing greatly to the development of overproducing industrial strains overproducing proteins or secondary metabolites. I will briefly review 1) filamentous fungi as a host for production of recombinant proteins and secondary metabolites, 2) markets of industrial metabolites, 3) a new approach to manipulate up to five genes at the same time in the system that ProxEnrem uses.

• Molecules and Cells
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• v.43 no.7
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• pp.591-599
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• 2020

Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.

• International Commerce and Information Review
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• v.6 no.2
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• pp.385-400
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• 2004

China has pegged its currency to the US dollar (at RMB 8.3 to the dollar) since 1994. The yuan's dollar peg has increased demand for chinese exports, which account for about a third of gross domestic product. The peg has also helped to attract $308 billion in foreign investment. But there has been criticism that China has over the past two years been engaging in protracted, large-scale intervention in one direction in exchange markets. According to many reports, RMB is undervalued by somewhere between 15 and 30% by manipulation. China may not want to float the currency at once, since doing so would have a dramatic and negative effect on the economy. However, there has so far been strong pressure from trading partners including the Unite States, Japan, EU. Considered all these things, China may eventually allow some changes in the Yuan's value. This may come in the form of widening a band of movement for the currency, rather than letting it float freely in the market.

• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.165-174
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• 2003

Xanthophylls are oxygenated carotenoids that serve a variety of functions in photosynthetic organisms and are essential for survival of the organism. Within the last decade, major nor advances have been made in the elucidation of the molecular genetics and biochemistry of the xanthophyll biosynthesis pathway. Microalgae, yeast, or other microorganisms produce some of the xanthophylls that are being commercially used due to their own color and antioxidant properties. Currently, only a few microalgae are being considered or already being exploitd for the production of high-value xanthophylls. However, new developments in molecular biology have important implications for the commercialization of microalgae, and make the genetic manipulation of the xanthophyll content of microalgae mure attractive for biotechnological purposes. Accordingly, the current review summarizes the general properties of xanthophylls in microalgae and the recent developments in the biotechnological production of xanthophylls.

• East Asian Economic Review
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• v.17 no.3
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• pp.243-260
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• 2013

The dominant academic literature about trade agreements maintains that they are only about national terms-of-trade manipulation and not at all about purely political concerns. Non-academic economists, commentators, and diplomats by contrast think that trade agreements are all about political concerns. There are two substantive and important distinctions between the two views. i Practitioners maintain that policymakers care virtually not at all about the terms of trade or about trade-tax revenue. ii Practitioners, unlike academics, maintain that trade-agreement negotiations themselves change the underlying political economy. Observation of actual trade policy measures, though not conclusive, suggests that the practitioners are right and that the academics are wrong.

• Journal of Microbiology and Biotechnology
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• v.31 no.7
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• pp.903-911
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• 2021

Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology. Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms. Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.

• Medical Lasers
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• v.10 no.4
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• pp.201-206
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• 2021

The recent development of new surgical techniques using lasers has increased the opportunities for open surgery involving minimal manipulation and faster and more accurate removal of lesions. The increasing use of laser technology requires nurses to play an extensive role. As assistants, nurses play an important role in maintaining the efficacy and safety of the laser device. In addition, they are also responsible for providing pre-and post-operative care to patients. Therefore, nurses should be aware of how to proceed with operative laser treatment for all surgical procedures and the steps for maintaining safety prior to, during, and after laser treatment. This review provides in-depth knowledge for nurses undertaking continuing education on lasers and patient care in the field of gynecology.