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http://dx.doi.org/10.14348/molcells.2020.0020

Mapping Cellular Coordinates through Advances in Spatial Transcriptomics Technology  

Teves, Joji Marie (Biotech Research and Innovation Centre (BRIC), University of Copenhagen)
Won, Kyoung Jae (Biotech Research and Innovation Centre (BRIC), University of Copenhagen)
Publication Information
Molecules and Cells / v.43, no.7, 2020 , pp. 591-599 More about this Journal
Abstract
Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.
Keywords
cellular communication; single-cell RNA; spatial transcriptomics; tissue architecture;
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