• Clinical and Experimental Reproductive Medicine
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• 제45권3호
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• pp.143-148
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• 2018

Objective: The favored method of preserving fertility in young female cancer survivors is cryopreservation and autotransplantation of ovarian tissue. Reducing hypoxia until angiogenesis takes place is essential for the survival of transplanted ovarian tissue. The aim of this study was to investigate the role of angiopoietin-1 (Angpt-1), angiopoietin-2 (Angpt-2), and vascular endothelial growth factor (VEGF) in ovarian tissue grafts that were cryopreserved using two methods. Methods: Ovarian tissues harvested from ICR mice were divided into three groups: group I (control), no cryopreservation; group II, vitrification in EFS (ethylene-glycol, ficoll, and sucrose solution)-40; and group III, slow freezing in dimethyl sulfoxide. We extracted mRNA for VEGF, Angpt-1, and Angpt-2 from ovarian tissue 1 week following cryopreservation and again 2 weeks after autotransplantation. We used reverse transcriptase-polymerase chain reaction to quantify the levels of VEGF, Angpt-1, and Angpt-2 in the tissue. Results: Angpt-1 and Angpt-2 expression decreased after cryopreservation in groups II and III. After autotransplantation, Angpt-1 and Angpt-2 expression in ovarian tissue showed different trends. Angpt-1 expression in groups II and III was lower than in group I, but Angpt-2 in groups II and III showed no significant difference from group I. The vitrified ovarian tissues had higher expression of VEGF and Angpt-2 than the slow-frozen ovarian tissues, but the difference was not statistically significant. Conclusion: Our results indicate that Angpt-2 may play an important role in ovarian tissue transplantation after cryopreservation although further studies are needed to understand its exact function.

• Molecules and Cells
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• 제43권3호
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• pp.228-235
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• 2020

The Drosophila transmembrane semaphorin Sema-1a mediates forward and reverse signaling that plays an essential role in motor and central nervous system (CNS) axon pathfinding during embryonic neural development. Previous immunohistochemical analysis revealed that Sema-1a is expressed on most commissural and longitudinal axons in the CNS and five motor nerve branches in the peripheral nervous system (PNS). However, Sema-1a-mediated axon guidance function contributes significantly to both intersegmental nerve b (ISNb) and segmental nerve a (SNa), and slightly to ISNd and SNc, but not to ISN motor axon pathfinding. Here, we uncover three cis-regulatory elements (CREs), R34A03, R32H10, and R33F06, that robustly drove reporter expression in a large subset of neurons in the CNS. In the transgenic lines R34A03 and R32H10 reporter expression was consistently observed on both ISNb and SNa nerve branches, whereas in the line R33F06 reporter expression was irregularly detected on ISNb or SNa nerve branches in small subsets of abdominal hemisegments. Through complementation test with a Sema-1a loss-of-function allele, we found that neuronal expression of Sema-1a driven by each of R34A03 and R32H10 restores robustly the CNS and PNS motor axon guidance defects observed in Sema-1a homozygous mutants. However, when wild-type Sema-1a is expressed by R33F06 in Sema-1a mutants, the Sema-1a PNS axon guidance phenotypes are partially rescued while the Sema-1a CNS axon guidance defects are completely rescued. These results suggest that in a redundant manner, the CREs, R34A03, R32H10, and R33F06 govern the Sema-1a expression required for the axon guidance function of Sema-1a during embryonic neural development.

• 한국음향학회지
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• 제43권1호
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• pp.1-8
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• 2024

수동형 소나의 성능을 측정하기 위해 주어진 환경과 시스템 변수 하에서 보통 최대 탐지거리를 고려한다. 음파가 해표면 또는 해저면과 필연적으로 접촉하는 천해에서는 표적탐지가 최대 탐지거리까지 유지되는 게 일반적이다. 그러나 심해에서는 음파가 해표면 또는 해저면과 접촉하지 않을 수도 있으며, 이 경우 음파가 도달하지 않는 음영구역이 존재할 수도 있다. 이 경우 최대 탐지거리만으로 각 소나의 탐지성능을 완전하게 기술하기 어려울 수 있다. 보다 완전한 탐지성능 기술을 위해 '탐지견고성(Robustness Of Detection, ROD)' 개념을 도입하고자 한다. 동해 연안에서 수괴의 공간적 분포와 최대 탐지거리 및 탐지견고성은 밀접한 관계가 있으며, 최대 탐지거리와 탐지견고성은 서로 반대의 공간적 변동을 보인다. 경험직교함수(Empirical Orthogonal Function, EOF)를 도입하여 수온의 시공간적 분포를 분석한 결과 첫 번째 모드는 전형적인 계절 변화를 보이고, 두 번째 모드는 혼합층 등의 세기 변화를 반영하는 것으로 추정된다. 이 두 모드가 전체 변화의 약 92 %를 설명한다. 수심 5 m와 100 m 표적을 가정하여 수동형 소나의 최대 탐지거리와 두 모드의 계절 변화의 상관관계를 분석하면 첫 두 모드와 높은 음의 상관계수(약 -0.9)를 보인다. 수온의 계절적 변화는 표층 ~ 수심 200 m에서 발생하며, 이에 따라 수심 100 m에 표적이 존재한다고 가정하여 수동소나를 수심 100 m 이상에서 운용할 경우 계절변화가 미미한 탐지성능을 기대할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제10권3호
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• pp.415-418
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• 2000

Productivity of a rice plant is greatly influenced by salt stress. One of the ways to achieve tolerance to salinity is to transfer genes encoding protective enzymes from other organisms, such as microorganisms. The bacterial gene, mtlD, which encodes mannitol-1-phosphate dehydrogenase (Mtl-DH), was introduced to the cytosol of a rice plant by an imbibition technique to overproduce mannitol. The germination and survival rate of the imbibed rice seeds were markedly increased by transferring the mtlD gene when it was delivered in either a pBIN19 or pBmin binary vector. When a polymerase chain reaction was performed with the genomic DNAs of the imbibed rice leaves as a template and with mtlD-specific primers, several lines were shown to contain an exogenous mtlD DNA. However, a reverse transcription (RT)-PCR analysis revealed that not all of them showed an expression of this foreign gene. This paper demonstrates that the growth and germination of rice plants transiently transformed with the bacterial gene, mtlD, are enhanced and these enhancements may have resulted from the experssion of the mtlD gene. The imbibition method empolyed in this study fulfills the requirements for testing the function of such a putative gene in vivo prior to the production of a stable transgenic plant.

• Molecules and Cells
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• 제37권10호
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• pp.734-741
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• 2014

Histone modifications on major transcription factor target genes are one of the major regulatory mechanisms controlling adipogenesis. Plant homeodomain finger 2 (PHF2) is a Jumonji domain-containing protein and is known to demethylate the histone H3K9, a repressive gene marker. To better understand the function of PHF2 in adipocyte differentiation, we constructed stable PHF2 knock-down cells by using the mouse pre-adipocyte cell line 3T3-L1. When induced with adipogenic media, PHF2 knock-down cells showed reduced lipid accumulation compared to control cells. Differential expression using a cDNA microarray revealed significant reduction of metabolic pathway genes in the PHF2 knock-down cell line after differentiation. The reduced expression of major transcription factors and adipokines was confirmed with reverse transcription- quantitative polymerase chain reaction and Western blotting. We further performed co-immunoprecipitation analysis of PHF2 with four major adipogenic transcription factors, and we found that CCATT/enhancer binding protein (C/EBP)${\alpha}$ and C/EBP${\delta}$ physically interact with PHF2. In addition, PHF2 binding to target gene promoters was confirmed with a chromatin immunoprecipitation experiment. Finally, histone H3K9 methylation markers on the PHF2-binding sequences were increased in PHF2 knock-down cells after differentiation. Together, these results demonstrate that PHF2 histone demethylase controls adipogenic gene expression during differentiation.

• Asian Pacific Journal of Cancer Prevention
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• 제15권9호
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• pp.4033-4037
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• 2014

Emerging evidence has shown associations of microRNA-205 (miR-205) with crucial cell processes such as the epithelial-mesenchymal transition (EMT) and aberrant expression with tumorigenesis in many types of human malignancy. This prospective study characterized the contribution of miR-205 to the colorectal cancer (CRC) tumorigenesis. The real-time reverse transcription-polymerase chain reaction was used to examine miR-205 levels prospectively in 36 pairs of samples of CRC tissue and adjacent noncancerous tissue (>2 cm from cancer tissue). In addition, the relationship between miR-205 levels and clinicopathological features was explored. The capability of miR-205 to function as a tumor marker was also examined. miR-205 expression levels did not show significant changes overall. However, miR-205 was significantly downregulated in a group of CRC samples compared with matched noncancerous tissue samples. Moreover, decreased miR-205 correlated significantly with lymphatic metastasis. A receiver operating characteristic (ROC) curve also showed an optimum cut off point of $1.4{\times}10^{-3}$ to distinguish lymphatic metastatic CRCs from non-metastatic CRCs. Interestingly we found lymphatic metastasis in almost 80% of the depressed samples. This study suggested that miR-205 could be reduced in the majority of metastatic CRCs and the risk of CRC metastasis may be predicted by monitoring miR-205 in patient samples collected at the time of the initial diagnosis. Therefore, targeting miR-205 and its potential environmental activators might be a promising therapeutic option to prevent malignant progression toward metastasis.

• 대한전자공학회논문지SD
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• 제42권1호
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• pp.39-46
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• 2005

본 연구에서는 광논리 및 광접속에 응용할 수 있는 GaAs/AIGaAs 구조의 완전 공핍 광 싸이리스터(depleted optical thyristor, DOT)에 1/4 파장 거울층 (quarter wavelength reflector stacks, QWRS)을 제작하여 특성을 측정 분석하였다. 바닥면에 위치한 QWRS는 광 방출 효율뿐만 아니라 흡수 효율을 증가시킨다. 바닥면에 QWRS를 넣은 것과 그렇지 않은 두가지의 DOT를 제작하여 비선형 S-자 형의 전류-전압 특성, 광 방출 효율 및 흡수 효율을 측정, 분석하였다. 하부 거울층을 삽입한 DOT와 기존의 DOT의 스위칭 변화는 각각 1.82 V와 1.52 V로 흡수효율에서 20 % 증가함을 보인다. 뿐만 아니라, 하부 거울층을 이용한 DOT는 기존의 소자에 비하여 발광 효율 면에서 최고 46 % 향상된 결과를 나타낸다. 스위칭 특성을 분석하기 위하여 순방향 전압에서 비선형 s-자형의 전류-전압 특성을, 역방향 전압에서 완전 공핍 전압을 모의실험을 통하여 알아보았다. 모의실험 방법으로 유한 차분 방법 (finite difference method, FDM)을 이용하여 최적화된 DOT 각 층의 두께와 도핑 농도를 구하였다.

• Archives of Plastic Surgery
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• 제44권3호
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• pp.210-216
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• 2017

Background In treating minor-form or microform cleft lip, obtaining an optimal result is a challenge because of the visible scarring caused by traditional surgery. We present a refined method using muscle overlapping with a minimal skin incision in patients younger than 3 years, a group characterized by thin muscle. Methods The surgical technique involves restoration of the notched vermillion using Z-plasty, formation of the philtral column using overlapping of an orbicularis oris muscle flap through an intraoral incision, and correction of the cleft lip nasal deformity using a reverse-U incision and V-Y plasty. A single radiologist evaluated ultrasonographic images of the upper lip. Results Sixty patients were treated between September 2008 and June 2014. The age at the time of operation ranged from 6 to 36 months (mean, 26 months). The follow-up period ranged from 8 to 38 months (mean, 20 months) in minor-form cases and from 14 to 64 months (mean, 37 months) in microform cases. A notched cupid's bow was corrected in 10 minor-form cases and 50 microform cases. Ultrasonographic images were obtained from 3 patients with minor-form cleft lip and 9 patients with microform cleft lip 12 months after surgery. The average muscle thickness was 4.5 mm on the affected side and 4.1 mm on the unaffected side. Conclusions The advantages of the proposed procedure include the creation of an anatomically natural philtrum with minimal scarring. This method also preserves the continuity and function of the muscle and provides sufficient augmentation of the philtral column and nostril sill.

• Clinical and Experimental Pediatrics
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• 제60권9호
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• pp.296-301
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• 2017

Purpose: The aim of this study was to evaluate whether infants with rhinovirus (RV) infection-induced wheezing and those with respiratory syncytial virus (RSV) infection-induced wheezing have different cytokine profiles in the acute stage. Methods: Of the infants with lower respiratory tract infection (LRTI) between September 2011 and May 2012, 88 were confirmed using reverse transcription polymerase chain reaction and hospitalized. Systemic interferon-gamma ($IFN-{\gamma}$), interleukin (IL)-2, IL-12, IL-4, IL-5, IL-13, and Treg-type cytokine (IL-10) responses were examined with multiplex assay using acute phase serum samples. Results: Of the 88 patients, 38 had an RV infection (RV group) and 50 had an RSV infection (RSV group). In the RV group, the $IFN-{\gamma}$ and IL-10 concentrations were higher in the patients with than in the patients without wheezing (P=0.022 and P=0.007, respectively). In the RSV group, the differences in $IFN-{\gamma}$ and IL-10 concentrations did not reach statistical significance between the patients with and the patients without wheezing (P=0.105 and P=0.965, respectively). The $IFN-{\gamma}$ and IL-10 concentrations were not significantly different between the RV group with wheezing and the RSV group with wheezing (P=0.155 and P=0.801, respectively), in contrast to the significant difference between the RV group without wheezing and the RSV group without wheezing (P=0.019 and P=0.035, respectively). Conclusion: In comparison with RSV-induced LRTI, RV-induced LRTI combined with wheezing showed similar $IFN-{\gamma}$ and IL-10 levels, which may have an important regulatory function.

• 한국측량학회지
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• 제33권1호
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• pp.1-9
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• 2015

원격탐사영상의 기상조건과 계절에 따른 모의는 영상 전처리 단계 중 대기보정 알고리즘의 역방향 구현을 통해 수행될 수 있다. 본 연구는 상용 원격탐사영상처리 프로그램에서 제공되는 대기보정기능으로 보정된 Landsat영상을 특정기상조건이 고려된 대기보정 이전 상태로 모의하는 방법을 실험하였다. 실험에 적용한 방법은 Forster 알고리즘(1984)과 6S RTM (Radiative Transfer Model) 이고, 모의된 결과영상을 특정기상조건의 원영상과 비교하여 일치성을 분석하였다. 6S RTM을 적용한 결과가 Forster 알고리즘보다 일치성이 높았고, 결과연구대상지역 영상으로 모의영상을 제작한 결과, 원영상과의 평균 RMSE of DN difference가 9.35이고, 평균 $R^2$는 0.7이였다. 결과적으로 참조 영상과 계절이 동일하고 시기가 유사한 모의영상 생성 시 실용적 활용 가능성을 입증하였다.