• 운동영양학회지
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• 제24권4호
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• pp.15-23
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• 2020

[Purpose] Lactate is a principal energy substrate for the brain during exercise. A single bout of high-intensity interval exercise (HIIE) can increase the blood lactate level, brain lactate uptake, and executive function (EF). However, repeated HIIE can attenuate exercise-induced increases in lactate level and EF. The lactate levels in the brain and blood are reported to be correlated with exercise-enhanced EF. However, research is yet to explain the cause-and-effect relationship between lactate and EF. This study examined whether lactate consumption improves the attenuated exercise-enhanced EF caused by repeated HIIE. [Methods] Eleven healthy men performed two sets of HIIE, and after each set, 30 min were given for rest and examination. In the 2^nd set, the subjects consumed experimental beverages containing (n = 6) and not containing (n = 5) lactate. Blood, cardiovascular, and psychological variables were measured, and EF was evaluated by the computerized color-word Stroop test. [Results] The lactate group had a higher EF (P < 0.05) and tended to have a higher blood lactate level (P = 0.082) than the control group in the 2^nd set of HIIE. Moreover, blood lactate concentration was correlated with the interference score (i.e., reverse score of EF) (r = -0.394; P < 0.05). [Conclusion] Our results suggest that the attenuated exercise-enhanced EF after repeated HIIE can be improved through lactate consumption. However, the role of lactate needs to be elucidated in future studies, as it can be used for improving athletes' performance and also in cognitive decline-related clinical studies.

• 한국발생생물학회지:발생과생식
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• 제18권3호
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• pp.161-166
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• 2014

Previously, we demonstrated that the shift and/or restriction of feeding time during relatively short-term period (4 weeks) could alter the pituitary gonadotropin expression and the weights of seminal vesicle and prostate in rats. We also found that the reverse feeding (RF) schedule (up to 8 weeks) might induce an adaptable metabolic stress and cause impairment of androgen-dependent reproductive tissues. In the present study, we extended the RF time regimen up to 12 weeks, and measured the reproductive tissue weights. After 4 and 8 weeks of RF, the weights of epididymis were not significantly different. After 12 weeks, however, epididymis weights of RF animals were significantly different (CON 12W : RF 12W = $48.26{\pm}0.62mg$ : $44.05{\pm}1.57mg$, p<0.05). After 4 and 12 weeks of feeding, seminal vesicle weights of RF animals were significantly decreased (CON 4W : RF 4W = $79.36{\pm}8.34mg$ : $46.28{\pm}2.43mg$, p<0.001; CON 12W : RF 12W = $72.04{\pm}3.76mg$ : $46.71{\pm}2.27mg$, p<0.001, respectively). Prostate weights were not changed by RF. Kidney and spleen weights of RF animals were significantly different on weeks 4 and 12 (Kidney, CON 4W : RF 4W = $249.72{\pm}4.20mg$ : $228.41{\pm}3.03mg$, p<0.001; CON 12W : RF 12W = $309.15{\pm}7.49mg$ : $250.72{\pm}6.13mg$, p<0.001, respectively, Spleen, CON 4W : RF 4W = $111.26{\pm}3.76mg$ : $96.88{\pm}4.69mg$, p<0.05; CON 12W : RF 12W = $123.93{\pm}10.72mg$ : $94.68{\pm}5.65mg$, p<0.05, respectively). Histology analysis of seminal vesicle revealed that the thinner epithelial cell layers, reduced complexities of swollen papilla folding in the exocrine glands on weeks 4 and 12 of RF. There was no histological difference between control and RF group on week 8. The present study indicates that up to 12 weeks RF induced differential changes in tissue weights of male mice. In particular, seminal vesicle, kidney and spleen seemed to temporarily adapted to the RF-induced metabolic stress on week 8 of feeding schedule. These results confirmed the our previous study that the RF might induce an adaptable metabolic stress and cause impairment of androgen-dependent reproductive tissues such as epididymis and seminal vesicle as well as non-reproductive tissues such as kidney and spleen. Further studies will be needed to achieve a better understanding of the how does mealtime shift affect the reproductive function and exact nature of adaptation.

• 한국미생물·생명공학회지
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• 제43권1호
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• pp.1-8
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• 2015

돼지생식기호흡기증후군(porcine reproductive and respiratory syndrome; PRRS) 바이러스의 ORF5a 단백질이 바이러스 생장에 필수적인 단백질인지 확인하기 위해서 PRRS 바이러스 감염성 클론을 이용하여 ORF5a 단백질 유전자를 결손시킨 변이 클론을 제작하였다. 야생형 PRRS 바이러스 감염성 클론과 ORF5a 단백질이 결손된 변이 클론을 BHK-tailless pCD163 세포에 transfection시킨 결과 변이클론에서감염성있는바이러스가숙주세포로부터만들어지지않았다. 이결과가 ORF5a 단백질발현의부재때문인지검증하기위해서 BHK-tailless pCD163-tailless 세포에 ORF5a 단백질을안정적으로발현하는세포주를제작하였고이세포주에동일한 transfection 실험을한결과세포에서공급되는 ORF5a 단백질발현에의해감염성있는바이러스가만들어지는것을확인하였다. 이로써 ORF5a 단백질이 PRRS 바이러스가 생장하는데 있어서 필수적인 단백질임을 확인할 수 있었다.

• Clinics in Shoulder and Elbow
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• 제17권2호
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• pp.68-76
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• 2014

Background: Recently, reverse total shoulder arthroplasty (RTSA) has been accepted as a main treatment option in irreparable massive rotator cuff tear with cuff arthropathy. The purpose of this study was to evaluate the early complication incidence and the preliminary clinical results of RTSAs performed in single institute. Methods: Fifty-seven RTSAs (56 patients) were performed between April 2011 and March 2013. The indications for RTSA were cuff tear arthropathy and irreparable massive rotator cuff tear with or without pseudoparalysis. Exclusion criteria were revision, preoperative infections and fractures. At final follow-up, 45 shoulders were enrolled. Mean follow-up duration was 12.5 months (range, 6-27 months). The mean age at the time of surgery was 73.6 years (range, 58-87 years). All the patients were functionally accessed via Constant score, American Shoulder and Elbow Surgeons (ASES) score, pain and functional visual analogue scale (VAS) scores and active range of motion. Complications were documented as major and minor. Major complications include fractures, infections, dislocations, nerve palsies, aseptic loosening of humeral or glenoid components, or glenoid screw problems. Minor complications include radiographic scapular notching, hematomas, heterotopic ossification, algodystrophy, intraoperative dislocations, intraoperative cement extravasation, or radiographic lucent lines of the glenoid. Results: The mean Constant score increased from 31.4 to 53.8 (p < 0.001). The pain and functional VAS scores improved (5.2 to 2.7, p < 0.001, 4.0 to 6.7, p < 0.001) and active forward flexion improved from $96.9^{\circ}$ to $125.6^{\circ}$ (p = 0.011). One or more complications occurred in 16 (35.6%) of 45 shoulders, with one failure (2.2%) resulting in the removal of implants by late infection. The single most common complication was scapular notching (9 [20%]). There were 4 (8.9%) axillary nerve palsies postoperatively (n=3: transient n. palsy, n=1: Symptom existed at 11 months postoperatively but improving). Conclusions: In a sort term follow-up, RTSA provided substantial gain in overall function. Most common early complications were scapular notching and postoperative neuropathy. Although overall early complication rate was as high as reported by several authors, most of the complications can be observable without compromise to patients' clinical outcome. Long term follow-up is required to clarify the clinical result and overall complication rate.

• 한국항행학회논문지
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• 제16권2호
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• pp.360-366
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• 2012

공항과 같은 산업 설비에 사용되는 분전반에서는 주 개폐기로 배선용 차단기가 사용이 되고 있으며, 분기 개폐기는 누전 차단기가 사용되어 인체 보호 및 누전 화재 보호 기능을 수행하고 있을 뿐만 아니라, 과전류 보호, 단락 보호 겸용 기능을 포함하여 사용되어 지고 있다. 특히 공항용 분전반의 경우 사용자의 급증과 함께 급속한 첨단화와 기기의 대용량화, 다양화, 전원용량의 증대 등으로 공항의 안정적인 전원 공급을 위하여 사고에 대한 보호 증대가 필요하게 되었고, 분기 누전 차단기의 다량 사용에 의한 2열 배열의 접속 방법 등으로 분전반을 제작하여 설치 면적에 대한 이슈가 부각되어 차단기의 슬림화가 주요한 문제로 자리 잡고 있다. 본 논문에서는 이를 위하여 아크 소호 기구부의 설계, 접점이 운동 방향 변경을 고려하여 기구부를 설계하고, 누전 검출 회로의 소형화 및 역 접속 시에도 안적적인 동작이 가능하도록 설계하였으며, 이를 검증하기 위하여 단락 시험을 실시함으로써 성능 검증을 하였고, 사고 전류에 대한 보호 기능의 강화와, 차단기의 슬림화, 역접속시에서 누전 동작이 가능한 차단기를 개발함으로써 급속히 커져가는 공항용 분전반에서 발생하는 공간 사용의 문제점을 해결하는데 도움을 주고자 한다.

• 방송공학회논문지
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• 제18권1호
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• pp.66-76
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• 2013

최근 다양한 환경에서 사용할 수 있는 3D 디스플레이가 활발하게 보급되었으나, 3D 컨텐츠의 보급은 그에 미치지 못하고 있다. 3D 콘텐츠의 확산을 저지하는 원인으로 주로 언급되는 것은 시각 피로이지만, 그에 못지않게 영상 왜곡의 영향 역시 크다. 신윤호, 이형철, 김신우 (2012)는 앞선 연구에서 단순한 자극에 대한 크기 지각 왜곡 효과를 측정하고 화면 시차와 크기 지각 사이에 선형적 관계가 존재함을 보고하였다. 본 연구에서는 이에 더하여 정사각형, 자유 선, 입방체 등 다양한 자극 특성이 자극 크기 지각에 미치는 영향을 크기 맞추기 과제를 사용하여 측정하였다. 그 결과 앞선 연구의 결과와 마찬가지로 화면 시차에 따라 지각된 크기가 선형적으로 변화하는 것을 확인하였으며, 이러한 선형성은 대상의 종류와 무관하게 일관되게 관찰되었다. 화면시차와 크기지각의 관계를 시각도(visual angle)에 의한 크기변화 예측함수로 제시하였다. 이 함수를 사용하면 영상제작에서 특정 화면시차의 자극이 시청자에게 어떤 크기로 지각될 지를 미리 예측할 수 있어 매우 유용할 것으로 기대한다.

• Animal Bioscience
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• 제36권1호
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• pp.144-155
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• 2023

Objective: Adipocyte differentiation is regulated by a variety of functional genes and noncoding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear. Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods: The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened and gene ontology (GO) and Kyoto encyclopedia for genes and genomes (KEGG) analyses were performed. Gain-of-function and loss-of-function combined with oil red O staining, Bodipy staining, and quantitative reverse-transcription polymerase chain reaction (qPCR) were utilized to determine the effect of miR-133a-3p on adipocyte differentiation. Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and CCAAT enhancer binding protein α (C/EBPα) and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (p<0.01), the loss-of-function also led to a significant down-regulation of peroxisome proliferator activated receptor gamma (PPARγ) (p<0.01). Conclusion: This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1229-1233
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• 2006

To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2003년도 춘계학술발표강연 및 논문개요집
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• pp.88-88
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• 2003

Optical Emission Spectroscopy(OES) is a very important technology for real-time monitoring of plasma in a reactor during dry etching process. OES technology is non-invasive to the plasma process. It can be used to collect information on excitation and recombination between electrons and ions in the plasma. It also helps easily diagnose plasma intensity and monitor end-point during plasma etch processing. We studied high density planar inductively coupled BCl$_3$ and BCl$_3$/Ar plasma with an OES as a function of processing pressure, RIE chuck power, ICP source power and gas composition. The scan range of wavelength used was from 400 nm to 1000 nm. It was found that OES peak Intensity was a strong function of ICP source power and processing pressure, while it was almost independent on RIE chuck power in BCl$_3$-based planar ICP processes. It was also worthwhile to note that increase of processing pressure reduced negatively self-induced dc bias. The case was reverse for RIE chuck power. ICP power and gas composition hardly had influence on do bias. We will report OES results of high density planar inductively coupled BCl$_3$ and BCl$_3$/Ar Plasma in detail in this presentation.

• 한국지구과학회지
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• 제35권1호
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• pp.69-87
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• 2014

이 논문에서는 구성 자료의 지구통계학적 시뮬레이션을 위해 다중 자료 변환 기반 조건부 시뮬레이션 틀을 제안하였다. 우선 일반적인 통계 기법의 적용이 가능하도록 구성 자료에 로그비 변환을 적용하였다. 다음 변환들로는 최소/최대 자기상관 인자 변환과 지시자 변환을 순차적으로 적용하였다. 독립적인 새로운 변수의 생성을 위해 최소/최대 자기상관 인자 변환을 적용하였으며, 적용 결과 개별 변수들의 독립적인 시뮬레이션이 가능해진다. 그리고 다중 가우시안 확률 모델을 따르지 않는 변수들의 비모수적 조건부 누적 확률 분포 모델링을 위해 지시자 변환을 적용하였다. 최종적으로는 적용한 변환 방법들의 역순으로 역 변환을 적용하였다. 간석지 표층 퇴적물 성분 자료를 대상으로 제안 시뮬레이션 기법의 적용 가능성을 예시하였다. 모든 시뮬레이션 결과들은 구성 자료의 제한 조건을 만족하면서 샘플 자료의 통계 특성을 잘 반영하였다. 구성 자료의 다수의 시뮬레이션 결과들을 이용한 표층 퇴적물 분류를 통해 기존 크리깅에서는 얻을 수 없는 분류 결과의 확률론적 평가가 가능하였다. 따라서 제안 시뮬레이션 틀은 다양한 구성 자료의 지구통계학적 시뮬레이션에 효과적으로 이용될 수 있을 것으로 기대된다.