• Explosives and Blasting
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• v.26 no.2
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• pp.38-44
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• 2008

Recently, planning road construction in South Korea is focused on upgrading of the existing road by rerouting or restructuring. For this, roads under current construction in Korea go for more straight in its alignments and routing. Straight routing makes it all the more required to construct many mountain tunnels and bridges in Korea where mountains are so widely spread. Some portal of mountain tunnel is not rarely planed at high steep slope of mountain valley where it is not easy to secure working space for tunnel excavation. Reverse excavation is an alternative measure for excavation of tunnel portal at high steep slope. Construction in reverse excavation method has three important points requiring careful consideration: 1)planning of pilot tunnel in proper width, height, and length etc., 2)measure against the effect of one-side earth pressure to the direction of tunnel portal, 3)securing tunnel safety against shallow ground condition at portal zone. This paper intends to suggest applicable range of pilot tunnel for reverse excavation at the portal zone located at high steep slope, and shows result of study on the appropriateness of a reverse excavation by means of 3D numerical analysis. Result of 3D numerical analysis for reverse excavation at high steep slope shows that pilot tunneling will be applicable to start from the point $20{\sim}25m$ before the portal from inside the tunnel.

• Radiation Oncology Journal
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• v.23 no.1
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• pp.43-50
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• 2005

Purpose : A number of genes and their products are Induced early or late following exposure of cells to ionizing radiation. These radiation-Induced genes have various effects on irradiated cells and tissues. Suppression subtractive hybridization (SSH) based on PCR was used to Identify the differentially expressed genes by radiation in cervix carcinoma cells. Materials and Methods : Total RNA and poly $(A)^+$ mRNA were Isolated from Irradiated and non-irradiated HeLa cells. Forward- and reverse-subtracted cDNA libraries were constructed using SSH. Eighty-eight clones of each were used to randomly select differentially expressed genes using reverse Northern blotting (dot blot analysis). Northern blotting was used to verify the screened genes. Results : Of the 17t clones, 10 genes in the forward-subtracted library and 9 genes In the reverse-subtracted library were identified as differentially expressed radiation-induced genes by PCR-select differential screening. Three clones from the forward-subtracted library were confirmed by Northern blotting, and showed increased expression in a dose-dependent manner, including a telomerase catalytic subunit and sodium channel-like protein gene, and an ESTs (expressed sequence tags) gene. Conclusion : We Identified differentially expressed radiation-induced genes with low-abundance genes with SSH, but further characterization of theses genes are necessary to clarify the biological functions of them.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3735-3742
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• 2013

Background: Cardamom (Elettaria cardamom), also known as "Queen of Spices", has been traditionally used as a culinary ingredient due to its pleasant aroma and taste. In addition to this role, studies on cardamom have demonstrated cancer chemopreventive potential in in vitro and in vivo systems. Nevertheless, the precise poly-pharmacological nature of naturally occurring chemo-preventive compounds in cardamom has still not been fully demystified. Methods:In this study, an effort has been made to identify the proapoptopic, anti-inflammatory, anti-proliferative, anti-invasive and anti-angiogenic targets of Cardamom's bioactive principles (eucalyptol, alpha-pinene, beta-pinene, d-limonene and geraniol) by employing a dual reverse virtual screening protocol. Experimentally proven target information of the bioactive principles was annotated from bioassay databases and compared with the virtually screened set of targets to evaluate the reliability of the computational identification. To study the molecular interaction pattern of the anti-tumor action, molecular docking simulation was performed with Auto Dock Pyrx. Interaction studies of binding pose of eucalyptol with Caspase 3 were conducted to obtain an insight into the interacting amino acids and their inter-molecular bondings. Results:A prioritized list of target proteins associated with multiple forms of cancer and ranked by their Fit Score (Pharm Mapper) and descending 3D score (Reverse Screen 3D) were obtained from the two independent inverse screening platforms. Molecular docking studies exploring the bioactive principle targeted action revealed that H- bonds and electrostatic interactions forms the chief contributing factor in inter-molecular interactions associated with anti-tumor activity. Eucalyptol binds to the Caspase 3 with a specific framework that is well-suited for nucleophilic attacks by polar residues inside the Caspase 3 catalytic site. Conclusion:This study revealed vital information about the poly-pharmacological anti-tumor mode-of-action of essential oils in cardamom. In addition, a probabilistic set of anti-tumor targets for cardamom was generated, which can be further confirmed by in vivo and in vitro experiments.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.155-161
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• 2001

A bactriocin produced by Lactrococcus sp. HY449 was purified by sequential purfication steps such as n-propanol-acetone precipitation ion -exchange chromatography using CM-Sequential CL6B. gel filtration chromatography using Sephacry HR100 and reverse-phase chromatography using pro RPC HR 5/10. Reverse-phase chromatography the final step of the purfication yielded a single symmetrical peak of bacteriocin activity The purification resulted in final yield of 3.25% and 413.35 fold increase of the specific activity of bacteriocin. The active fraction from reverse-phase chromatography was used for N-terminal amino acid analysis . The purified bacteriocin contained isoleucine, leucine, methionine, and glycine at but N-terminal end no aromatic amino acids. Calculation of the number of amino acid residues in the bacteriocin revealed that it is consisted of 32 residues assuming the molecular weight of bacteriocin to be about 3.6kDa. Edman degrandation elucidated amino acid residues of the first four of the N-terminus to be $NH_2$-Ile-Leu-Pro-GIn.

• Journal of Family Resource Management and Policy Review
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• v.15 no.4
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• pp.169-188
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• 2011

The primary focus of this study was on the issues associated with family and economic decision-making, in particular for the elderlyhood which might have to (or could be) share the family resources. This study regarded willingness to consider applying for Reverse Mortgage as an economic decision-making. The data was collected by interviewing 320 persons (over age 55) living in metropolitan areas in 2008. We selected 227 cases to find the influence of family, having both spouse and adult-children. We have used a theory of Planned Behavior by Fishbein to analysis three research questions. The results are as follows : first, the results partially explain willingness to consider applying for reverse mortgage using Fishbein's theory. Second, several results indicated that economic decision-makings within the family are influenced by several factors relating to other family member's view. They means that some of Subjective Norm variables, of Attitude Behavior ones, and of Perceived Behavior Control ones. Third, the result shows men and women respond differently to consider to applying for that policy. Men are conscious of the other people, i.e., friends, relatives except close family members, and, on the other hand, women are mindful of close family members, i.e., adult children. Forth, the group who have lower income showed higher intentions to consider that policy, when they have adult-children. Fifth, according to age group, pre-elderly group are influenced by consent of adult-children, a level of education, and on the other hand, elderly group are influenced by the other people, i.e., friends, relatives, a level of education, and so on. These results meaned that these distinct characteristics should be considered to establish Income Security policy for the pre-elderly and the elderly.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.9
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• pp.111-119
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• 2008

As the penetration of distributed generation systems is recently high, there have been metering errors, trips of protective devices in KEPCO distribution systems including an occurrence of false fault-indicator in distribution automation system. The cause of malfunctions was the reverse-power-flow phenomenon due to transformer wiring types. By the effect of the reverse-power-flow, each of phase's fundamental currents was added by about 3 times on the neutral line. A new method based on the analysis of the reverse-power-flow is proposed in this paper. Fault currents on each section were analyzed by the proposed method, and the effect of types of transformer wiring was examined experimentally. In order to reduce the malfunctions due to the reverse-power-flow, controlling the zero-sequence impedance of transformer was designed and verified by using PSCAD/EMTDC software.

• Computers and Concrete
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• v.33 no.5
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• pp.595-603
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• 2024

In the construction industry, there has been a surge in the implementation of high-tech equipment in recent years. Various technologies are being considered as potential solutions for future construction projects. Building information modeling (BIM), which utilizes advanced equipment, is a promising solution among these technologies. The need for safety inspection has also increased with the aging structures. Nevertheless, traditional safety inspection technology falls short of meeting this demand as it heavily relies on the subjective opinions of workers. This inadequacy highlights the need for advancements in existing maintenance technology. Research on building safety inspection using 3D laser scanners has notably increased. Laser scanners that use light detection and ranging (LiDAR) can quickly and accurately acquire producing information, which can be realized through reverse engineering by modeling point cloud data. This study introduces an innovative evaluation system for building safety using a 3D laser scanner. The system was used to assess the safety of an existing three-story building by implementing a reverse engineering technique. The 3D digital data are obtained from the scanner to detect defects and deflections in and outside the building and to create an as-built BIM. Subsequently, the as-built structural model of the building was generated using the reverse engineering approach and used for structural analysis. The acquired information, including deformations and dimensions, is compared with the expected values to evaluate the effectiveness of the proposed technique.

• Tunnel and Underground Space
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• v.17 no.3 s.68
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• pp.165-174
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• 2007

The technique, which reproduces the figures of objects from measured data of the objects using 3-D laser scanner, is called reverse engineering. Recently, research studies into applications of reverse engineering to rock engineering are increasing in number, in the discontinuity surveys for rock slopes out of man's reach, or rapid discontinuity surveys for wide range areas. For analysis of discontinuity using reverse engineering, a program for processing point clouds data from the 3-D laser scanner, for sampling from these point clouds data, and finally analyzing the discontinuity is needed. However, existing programs rarely have sufficient functions to properly analyze the discontinuities. In this study, a program was developed, which can automatically sample discontinuities from the point clouds data which measured in a rock slope using a 3-D laser scanner, and which can also undertake statistical analysis of the discontinuities. This developed program was verified by the application of discontinuity surveys in a rock slope and a tunnel. By undertaking the discontinuity survey using a 3-D laser scanner and the developed program, the feasibility and rapidity of such surveys is expected to improve in areas out of man's reach in geotechnical surveys. Taking into consideration the fact that the international level of related techniques is at a rudimentary stage, the possibility of prior occupation of a broad market is also expected.

• Earthquakes and Structures
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• v.11 no.3
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• pp.517-538
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• 2016

This paper presents a probabilistic fragility analysis for two groups of bridges: simply supported and integral bridges. Comparisons are based on the seismic fragility of the bridges subjected to accelerograms of two seismic sources. Three-dimensional finite-element models of the bridges were created for each set of bridge samples, considering the nonlinear behaviour of critical bridge components. When the seismic hazard in the site is controlled by a few seismic sources, it is important to quantify separately the contribution of each fault to the structure vulnerability. In this study, seismic records come from earthquakes that originated in strike-slip and reverse faulting mechanisms. The influence of the earthquake mechanism on the seismic vulnerability of the bridges was analysed by considering the displacement ductility of the piers. An in-depth parametric study was conducted to evaluate the sensitivity of the bridges' seismic responses to variations of structural parameters. The analysis showed that uncertainties related to the presence of lap splices in columns and superstructure type in terms of integral or simply supported spans should be considered in the fragility analysis of the bridge system. Finally, the fragility curves determine the conditional probabilities that a specific structural demand will reach or exceed the structural capacity by considering peak ground acceleration (PGA) and acceleration spectrum intensity (ASI). The results also show that the simply supported bridges perform consistently better from a seismic perspective than integral bridges and focal mechanism of the earthquakes plays an important role in the seismic fragility analysis of highway bridges.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2923-2928
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• 2015

Background: Gallbladder carcinoma (GBC) has been stated as an Indian disease, with the highest number of cases being reported from certain districts of northeast India, which has an ethnically distinct population. Unfortunately there are no scientific reports on the underlying molecular mechanisms associated with the pathogenesis of the disease from this region. Aim: The present study evaluated the role of differential expression of human telomerase reverse transcriptase (hTERT) in the development of gall bladder anomalies. Materials and Methods: Blood and tissue samples were collected from patients undergoing routine surgical resection for clinically proven cases of gallbladder disease {cholelithiasis (CL, n=50), cholecystitis (CS, n=40) and GBC (n=30) along with adjacent histopathologically proved non-neoplastic controls (n=15)} with informed consent. Whole blood was also collected from age and sex matched healthy controls (n=25) for comparative analysis. Differential hTERT mRNA expression was evaluated by semi-quantitative rt-PCR and real-time PCR based analysis using ${\beta}$-actin as an internal control. Evaluation of differential hTERT protein expression was studied by Western blot analysis and immunoflourescence. Statistical analysis for differential expression and co-relation was performed by SPSSv13.0 software. Results: Gallbladder anomalies were mostly prevalent in females. The hTERT mRNA and protein expression increased gradiently from normal