• 한국어병학회지
• /
• 제37권1호
• /
• pp.49-60
• /
• 2024

A viable but non-culturable (VBNC) state is a survival strategy adopted by bacteria when faced with unfavorable environmental conditions, rendering them unable to grow on nutrient agar while maintaining low metabolic activity. This study explored the impact of temperature and nutrient availability on inducing VBNC state in Edwardsiella piscicida, the most important bacterial fish pathogen, and assessed its pathogenicity at VBNC state. E. piscicida was suspended in filtered sterile seawater and exposed to three different temperatures (4, 10, and 25℃) to induce the VBNC state. Subsequently, the induced VBNC cells were subjected to resuscitation by either raising the temperature to 28℃ or inoculating them in brain heart infusion broth supplemented with 1% NaCl. A propidium monoazide (PMA)-qPCR method was also developed to selectively quantify live (VBNC or culturable) E. piscicida cells. The results showed that the bacteria entered the VBNC state after approximately 1 month at 4℃ and 25℃, and 2 months at 10℃. The VBNC E. piscicida cells were successfully revived within 3 days in a nutrient-rich environment at 28℃, highlighting the significance of temperature and nutrition in inducing and resuscitating the VBNC state. In pathogenicity tests, resuscitated E. piscicida cells exhibited high pathogenicity in olive flounder comparable to cultured bacteria, while VBNC cells showed no signs of infection, suggesting they are unlikely to resuscitate in fish. In conclusion, this study contributes to our understanding of fish pathogen ecology by investigating the characteristics of the VBNC state under varying temperature and nutrition conditions.

• Journal of Trauma and Injury
• /
• 제29권4호
• /
• pp.139-145
• /
• 2016

Purpose: The major pelvic trauma results in high mortality with associated fatal other injuries. During early stage of resuscitation, multidisciplinary approach is essential to improve the survival and outcomes. This study aims to report the effect and positive outcome of the trauma team approach on the management of hemodynamically unstable pelvic bone fracture. Methods: This retrospective review included all patients with hemodynamically unstable pelvic bone fracture admitted between March 2007 and December 2015. Patients were divided into group A, which comprised those admitted before the trauma team approach was started, and group B, which comprised those admitted after the approach was started. The advanced trauma life support protocol was followed for all patient. The comparisons between the two groups were based on medical records. Study variables included demographics, initial vital sign, injury severity score, fracture type, and injury mechanism. We analyzed the outcomes in each group with respect to the time interval for doctors' arrival, total length of stay in the emergency department (ED), time interval for computed tomography evaluation, 24-hour mortality, time interval for definitive fixation, and definitive fixation in the time-window of opportunity. Results: Fifty-three patients met the inclusion criteria. No statistically significant differences in demographic data existed between the two groups. The time interval for doctors' arrival (min, $63.09{\pm}50.48$ vs $21.48{\pm}17.75$; p=0.038) and total length of stay in the ED (min, $269.33{\pm}105.96$ vs $115.49{\pm}56.24$; p=0.023) were significantly improved. The 24-hour mortality was not significantly different between the two groups.(%, 14.3 vs 12.0; p=1.000) However, the time interval for definitive fixation and definitive fixation in the time-window of opportunity showed better results. Conclusion: The trauma team approach has positive effects, which include initial resuscitation through multidisciplinary approach and shortening the time interval to definitive fixation, on the management of hemodynamically unstable pelvic bone fracture.

• 한국임상보건과학회지
• /
• 제1권1호
• /
• pp.47-61
• /
• 2013

Purpose. Effective health policy to raise education rate and to provide basic data to identify the college of Education degree and CPR. Purpose of this study was to inform the need for retraining of college students received CPR training. Methods. The sample consisted of 70 a series of health related university students and 70 the general college students ; total 120 in J city. The period of data collecting was from November 1st to Nov. 16th. The tools were 24 questionnaires named "CPR Survey". The collected data were analyzed to get frequency, percentage, average, and standard deviation, t-test and Person's correlation coefficients by using of SPSS for WINDOW 12.0 K program. Results. The number of CPR-trained persons was significantly higher in the health related university students than general college students. Conclusions. CPR training after the passage of time, the percentage of correct answer got lower as time goes by. The answer should be 'School formal education' was the highest. The percentage of students who recognized the necessity of CPR re-education was high.

• 한국치위생학회지
• /
• 제17권6호
• /
• pp.1037-1051
• /
• 2017

Objectives: The purpose of this study was to examine the knowledge of dental hygienists on CPR, their CPR attitude and performance ability in an effort to offer data that can contribute to the development of a more effective CPR education program. Methods: A self-reported questionnaire was filled out by 234 dental hygienists in Jeollanam and Jeollabukdo from February 24 to May 20, 2017. The questionnaire asked dental hygienists of the knowledge, attitude, performance ability in regards to CPR. The data were analyzed using SPSS Window ver. 19.0 program through independent t-test, one-way ANOVA, chi-square test and multiple regression analysis. The Cronbach alpha of their CPR knowledge was 0.78, and that of attitude to CPR was 0.79. The Cronbach alpha of CPR performance ability was 0.96. Results: The dental hygienists surveyed in this study who were aware of CPR accounted for 88.9% of total subjects. Dental hygienists who were certified in CPR accounted for 20.5% of total subjects. They received a score of 7.66 on CPR knowledge, 3.33 on attitude and 2.61 on performance ability. There was a positive correlation between the knowledge and attitude, between the knowledge and performance ability and between the attitude and performance ability (r=0.332, r=0.461, r=0.426). A regression analysis showed that the dental hygienists who were younger, who graduated from a four-year university or a higher educational institution, who were CPR certificate holders, who received more CPR education, who were cognizant of automated external defibrillator, who were more knowledgeable on CPR and who took a more positive attitude were more likely to be affected in terms of CPR performance. Conclusions: The dental hygienists surveyed were aware of CPR on the whole, but their CPR knowledge, attitude and performance were not sufficient to perform CPR in emergency situations. More intensive education should be provided for dental hygienists to have an accurate knowledge of CPR to carry it out with a positive attitude.

• 한국산학기술학회논문지
• /
• 제13권4호
• /
• pp.1699-1705
• /
• 2012

본 연구는 심폐소생술 시행 시 구조자의 무릎이 바닥으로부터 10 cm 높은 위치와 바닥 위치 간에서 흉부압박의 질에 차이가 있는지를 알아보고자 시행하였다. G광역시의 G대학 응급구조과 1학년 재학생으로 심폐소생술 교육 과정을 이수한 66명 중, 무작위추출과정을 통해 2011년 11월 8일부터 9일까지 실험군 31명, 대조군 32명으로 선정하였다. 바닥으로부터 10 cm 위치(재질: B4 Copy Paper)와 바닥 위치에는 공통으로 매트리스(재질: PVC, 사이즈: $185{\times}125{\times}0.65cm$)를 깔고 2분 동안 흉부압박 만을 시행하였다. 실험 처치는 Resusci Anne 마네킨 1대를 사용하였으며, Laerdal PC SkillReporting System으로 시행결과를 기록하였다. 수집된 자료는 SPSS 14.0 for Window으로 $x^2$-test와 Fisher's exact probability test, Mann-Whitney U-test, Wilcoxon signed rank test를 사용하였다. 연구결과, 심폐소생술 구조자의 무릎이 바닥으로부터 10 cm 높은 위치가 바닥 위치보다 흉부압박 적절한 깊이(회), 평균 흉부압박 깊이(mm)가 더 효과적이었는데, 신장 170 cm 이하 그룹, 몸무게 65 kg 이하 그룹에서 흉부압박의 적절한 깊이(회), 평균 흉부압박 깊이(mm)가 효과적 이었다.

• Journal of Chest Surgery
• /
• 제13권3호
• /
• pp.212-218
• /
• 1980

A severe crushing injury of the chest produce a very striking syndrome referred to as traumatic asphyxia. This syndrome is characterized by bluish-red discoloration of the skin which is limited to the distribution of the valveless veins of the head and neck. And also if it is characterized by bilateral subconjunctival hemorrhages and neurological manifestations. But these clinical entities faded away progressively in a few weeks. Apporximately 90% of the patients who live for more than a few hours will recover from traumatic asphyxia when it occurs as a single entity. And so, death results from either severe associated injuries of from subsequent infection, rather than from pulmonary or cardiac insufficiency in traumatic asphyxia. We have experienced 4 cases of traumatic asphyxia with severe crushing thoracic injuries at department of the chest surgery, Captial Armed forces General Hospital during about 3 years from April 1977 to Aug. 1980. The 1st 22 year-old male was struct 2$\frac{1}{2}$ ton truck on the road and was transferred to this hospital immediately. He had taken tracheostomy due to severe dyspnea with contusion pneumonia and for removal of a large amount of bronchial secretion. The 2nd case was 23 year-old male who was got buried in a chasm. In this case, the heavy metal post tumbled over him back while at work. The 3rd case was 39 year-old male who leapt out of a window in 5th story while fire broke out in living room by oil stove heating. He had multiple rib fracture with right hemothor x and right colle's fracture and pelvic bone fracture. The last 22 year-old male was run over by a gun carriage. The wheel of this gun carriage passed over his thorax and right chin. He was brought to this hospital by helicopter. when he was first examined at emergency room, he was in semicomatose state and has pneurmomediastinum with multiple rib fracture and severe subcutaneous emphysema. As soon as he arrived, bilateral closed thoracostomy was performed and cardiopulmonary resuscitation was done. In hospital 8th weeks, chest series showed fibrothorax in right side even if chest wall stabilized. All 4 cases had multiple petechiae over their facees and chest and bilateral subconjunctival hemorrhages referred to as traumatic asphyxia. 3 cases except one case who received splenectomy, had been suffered from contusion pneumonia and had been treated with respiratory care. In these 3 cases, they had warning of impending injury before accident, and took a deep breath hold it and braces himself. And also, even if he had not impending fear in remaining one case, he had taken a deep breath and had got valsalva maneuver for pulling off the heavy metal post. Intrathoracic pressure rose suddenly and resulted to traumatic asphyxia in this situation. All these cases were recovered completely without sequelae except one fibrothorax, right.