• Title/Summary/Keyword: Response technology

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Optimization of Medium for Astaxanthin Production by Paracoccus sp. Using Response Surface Methodology (반응표면분석 법을 이용한 Paracoccus sp.의 Astaxanthin 생산배지 최적화)

  • Choi, Jong-Il;Lee, Hee-Sub;Choi, Seon-Kang;Kim, Jae-Hun;Kim, Jin-Kyu;Misawa, Norihiko;Byun, Myung-Woo;Lee, Ju-Woon
    • KSBB Journal
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    • v.24 no.3
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    • pp.321-326
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    • 2009
  • This study was to optimize the medium components for astaxanthin production in Paracoccus sp. through surface response methodology. A screening test was first conducted on 5 medium components using a Plackett-Burman design, from which $MgSO_4$ and yeast extract were identified as the significant factors affecting astaxanthin production. These significant factors were optimized by central composite design of experiments and response surface methodology, as 2.83 g/L $MgSO_4$ and 7.02 g/L yeast extract, respectively. The expected astaxanthin concentration with these optimized medium compositions were 0.925 mg/L. In flask culture, the experimentally obtained concentration of astaxantin was 1.021 mg/L, where it had been 0.4 mg/L before optimization.

Legal and Policy Tasks for Raising a Climate Fund in Response to a New Climate Regime (신기후체제 대응을 위한 기후기금 조성의 법·정책적 과제)

  • Ku, Ji Sun;Park, Chul Ho
    • Journal of Climate Change Research
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    • v.9 no.2
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    • pp.181-195
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    • 2018
  • On December 12, 2015, the Conference of the Parties to the United Nations Framework Convention on Climate Change (UNFCCC) adopted the Paris Agreement, in which several developed and developing countries all committed to participating in the reduction of greenhouse-gas (GHG) emissions. South Korea has submitted an intended nationally determined contribution (INDC) proposal with a target to cut down 37% greenhouse gas business as usual (BAU) until 2030 in preparation for the 2030 GHG BAU. Under the post-2020 regime, which will be launched from 2021 as the agreement entered into force early, it is expected that efforts to support GHG reduction and adaptation to climate change in developing countries will be accelerated with the utilization of technologies and financial resources of developed countries. South Korea has established the Basic Plan for Climate Change Response and the Basic National Roadmap for Greenhouse Gas Reductions by 2030 to promote the response to climate change at the government level. The Ministry of Science and ICT, as the National Designated Entity designated by the UNFCCC, has come up with middle and long-term strategies for climate technology cooperation. South-Korea has an abundance of energy-consuming industries to support its export-oriented industrial structure; it is thus expected that achieving the GHG reduction target will incur a considerable cost. Moreover, in order to meet the reduction target (11.3%) of the intended nationally determined contribution proposed by South Korea, it is necessary for South Korea to actively promote projects that can achieve GHG reduction achievements, and financial resources are needed as leverage to reduce risks that can occur in the early stages of projects and attract private sector investment. This paper summarizes the theoretical discussions on climate finance and conducted a comparative analysis on the status of the funds related to climate change response in the UK, Germany, Japan and Denmark. Through this, we proposed the legal and policy tasks that should be carried forward to raise public funds that can be used for creation of new industries related to climate change as well as to reduce GHG emissions in South Korea. The Climate Change Countermeasures Act, which has been proposed by the National Assembly of South-Korea, stipulates the establishment of funds but there is no additional funding except for general account. In this regard, it is also possible to take measures such as the introduction of carbon tax or the collection and use of royalties through technology research and development projects for climate change, such as Industrial Technology Innovation Promotion Act. In addition, since funds are used in various fields such as domestic greenhouse gas reduction, technology development, and overseas projects, it is necessary to establish a system in which various ministries cooperate with the operation of the fund.

Sequence Analysis of CO1 Genes of Fishery Resources from the Yellow Sea (eDNA 분석을 위한 황해 주요 수산자원의 CO1 염기서열 분석)

  • Hyun Sagong;Joo Myun Park;Yeonjung Lee;Wonseok Yang;Soo Jeong Lee;Maeng Jin Kim;Dong Han Choi
    • Ocean and Polar Research
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    • v.46 no.3
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    • pp.131-142
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    • 2024
  • Ocean change due to anthropogenic activities and climate change are causing a decline in coldwater fish species and emergence of subtropical fish species in Korean waters. Therefore, environmental change-dependent time-space distribution of fishery resources in Korea, which has a big fisheries industry, needs to be investigated. Environmental DNA (eDNA) metabarcoding is an environmentally noninvasive method for understanding the spatiotemporal distribution of marine organisms at high spatial resolution. The highly variable cytochrome oxidase-1 (CO1) gene is used in eDNA studies for species identification across diverse taxa. However, it exhibits genetic differences depending on geographical distribution. For improving the accuracy of eDNA research, the CO1 database should be expanded by incorporating sequence information for individuals inhabiting the Korean seas. Here, 106 biological samples from the Yellow Sea were identified morphologically and their nucleotide sequences were compared with those in the GenBank. Most sequences were 100% identical with those in the GenBank. In most samples, the morphological and molecular identification results were consistent, indicating the utility of CO1. However, some nucleotide sequences differed from those in the database. Amino acid sequences translated from nucleotide sequences with less than 97% similarity showed high similarity to the amino acid database, indicating intraspecies variation due to "silent mutations". These results highlight the need for a sequence database of fishery resources in Korean coastal waters to improve the reliability of eDNA studies using CO1. However, because of the same CO1 sequences in several species, genetic markers need to be developed and the database should be supplemented with more sequences for reliable high-resolution eDNA studies.

Extraction Yields and Functional Properties of Garlic Extracts by Response Surface Methodology

  • Lim, Tae-Soo;Do, Jeong-Ryong;Kwon, Joong-Ho;Kim, Hyun-Ku
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.379-383
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    • 2008
  • Extraction characteristics of garlic and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 26.41% was obtained at microwave power of 146.29 W, ethanol concentration of 63.31 %, and extraction time of 5.88 min. At microwave power, ethanol concentration, and extraction time of 114.84 W, 58.83%, and 1.42 min, respectively, maximum electron-donating ability (EDA) was 72.86%. Maximum nitrite-scavenging ability was 94.62% at microwave power, ethanol concentration, and extraction time of 81.83 W, 2.65%, and 3.83 min, respectively. Superoxide dismutase (SOD) showed maximum pseudo-activity of 49.12% at microwave power of 34.23 W, ethanol concentration of 33.11 %, and extraction time of 4.40 min. Based on superimposition of 4-dimensional RSM with respect to extraction yield, electron-donating ability, nitrite-scavenging ability, and pseudo-activity of SOD, optimum ranges of extraction conditions were microwave power of 0-100 W, ethanol concentration of 40-70%, and extraction time of 2-8 min.

Application of response surface methodology in pes/speek blend NF membrane for dyeing solution treatment

  • Lau, W.J.;Ismail, A.F.
    • Membrane and Water Treatment
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    • v.1 no.1
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    • pp.49-60
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    • 2010
  • In this study, response surface methodology (RSM) was performed in NF membrane process to evaluate the separation efficiency of membrane in the removal of salt and reactive dye by varying different variables such as pressure, temperature, pH, dye concentration and salt concentration. The significant level of both the main effects and the interaction were observed by analysis of variance (ANOVA) approach. Based on the statistical analysis, the results have provided valuable information on the relationship between these variables and the performances of membrane. The rejection of salt was found to be greatly influenced by pressure, pH and salt concentration whereas the dye rejection was relatively constant in between 96.22 and 99.43% regardless of the changes in the variables. The water flux on the other hand was found to be affected by the pressure and salt concentration. It is also found that the model predictions were in good agreement with the experimental data, indicating the validity of these models in predicting membrane performances prior to the real filtration process.

Physiochemical Changes and Optimization of Phosphate-Treated Shrimp (Litopenaeus vannamei) Using Response Surface Methodology

  • Omar, Saiah Djebbour;Yang, Je-Eun;Oh, Sang-Cheol;Kim, Dae-Wook;Lee, Yang-Bong
    • Preventive Nutrition and Food Science
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    • v.21 no.1
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    • pp.44-51
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    • 2016
  • The objective of this study was to determine the factors responsible for the changed physiochemical properties of unpeeled shrimp treated in cold phosphate solution ($2{\sim}4^{\circ}C$) with the intervention of 4 factors: phosphate concentration, dipping time, rotation speed, and volume of brine solution. Response surface analysis was used to characterize the effect of the phosphate treatment on shrimps by running 33 treatments for optimizing the experiment. For each treatment, phosphate amount, moisture content, and weight gain were measured. The results showed that phosphate concentration is the most important factor than other factors for facilitating phosphate penetration in the meat of the shrimp and for getting the best result. The optimum condition of phosphate-treated shrimp in this study was 110 to 120 min dipping time, 500 to 550 mL brine solution for 100 g shrimp sample, and 190 to 210 rpm agitation speed. The studied conditions can be applied in fisheries and other food industries for good phosphate treatments.

A Study on Requirements and Test Methods for the Safety of Electromyographs and Evoked Response Equipments (근전도계 및 유발성 응답기의 안전 요구사항 및 시험방법 연구)

  • Moon, I.H.;Nam, K.I.;Park, S.K.;Park, S.G.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.3 no.1
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    • pp.41-47
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    • 2009
  • This paper describes requirements and test methods for the safety of electromyographs and evoked response equipments to be used for medical examination of the central nerve system and motor-sensory functions in cortex. We first establish internationally harmonized requirements for the safety, and then propose a test procedure and test methods corresponding to the requirements based on the international standards. However requirements for the performance of each equipments are not specified in order to admit maker's specifications. Using a electromyography equipment we verified validity of the test methods proposed. Results of this study can be used as a standard for approval of Korea Food and Drug Administration (KFDA).

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Optimal Controller Design for Single-Phase PFC Rectifiers Using SPEA Multi-Objective Optimization

  • Amirahmadi, Ahmadreza;Dastfan, Ali;Rafiei, Mohammadreza
    • Journal of Power Electronics
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    • v.12 no.1
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    • pp.104-112
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    • 2012
  • In this paper a new method for the design of a simple PI controller is presented and it has been applied in the control of a Boost based PFC rectifier. The Strength Pareto evolutionary algorithm, which is based on the Pareto Optimality concept, used in Game theory literature is implemented as a multi-objective optimization approach to gain a good transient response and a high quality input current. In the proposed method, the input current harmonics and the dynamic response have been assumed as objective functions, while the PI controller's gains of the PFC rectifier (Kpi, Tpi) are design variables. The proposed algorithm generates a set of optimal gains called a Pareto Set corresponding to a Pareto Front, which is a set of optimal results for the objective functions. All of the Pareto Front points are optimum, but according to the design priority objective function, each one can be selected. Simulation and experimental results are presented to prove the superiority of the proposed design methodology over other methods.

Optimization of Ingredient for the Preparation of Asparagus cochinchinensis Makgeolli by Response Surface Methodology (반응 표면 분석을 이용한 천문동 첨가 막걸리 재료 혼합물의 최적화)

  • Kim, Ji Young;Park, Geum Soon
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.6
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    • pp.799-809
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    • 2013
  • This study was performed to determine the optimal composition of a makgeolli administered nuruk, water and Asparagus cochinchinensis. The experiment was designed base on BBD (box-behnken design), and an evaluation was carried out by means of RSM (response surface methodology), which included 15 experimental points with 3 replicates for the three independent variables nuruk, water and Asparagus cochinchinensis. The nuruk levels were tested in a range of 10~30 g, the water levels in a range 120~180% and Asparagus cochinchinensis was tested within a range of 2~6% by the weight of cooked-rice. Using the F-test, brix and appearance were expressed as a linear model, whereas the pH, acidity, DPPH radical scavenging, L-value, savory taste, taste, fresh aroma, after swallow and overall acceptability were expressed as a quadratic model. Increased amount of Asparagus cochinchinensis led to the reduction of the sensory scores for appearance, flavor, taste, texture and overall quality. The optimum formulation by numerical and graphical method were similar: nuruk 24.50 g, water 174.95% and Asparagus cochinchinensis 2.40%.

Hydrodynamic-Structural Response Coupling Analysis to a Rectangle Floating Structures (장방형 부유구조물에 대한 동유체력-구조응답 특성)

  • Oh, Young-Cheol;Gim, Ok-Sok;Ko, Jae-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.18 no.6
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    • pp.577-583
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    • 2012
  • Structures floating in the ocean experience various kinds of external loads, among which wave load is considered as determining factor in structural design. Its relative size compared with wavelength may be used to classify whether the structure is relatively small or large. Traditionally, the small structures are assumed to have little diffraction and the wave loads on large structure are usually calculated by only considering inertia force according to diffraction. In this paper, rectangular floating structures usually used in the ocean, river, and lake are used to find the relationship between hydrodynamic forces and its structural response.