• Journal of Life Science
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• v.33 no.7
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• pp.538-548
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• 2023

The research has been conducted on the isolation of antimicrobial compounds from plant natural extracts and their potential application in oral health care products. This study aimed to investigate the antimicrobial mechanism by analyzing the changes in gene expression of Streptococcus mutans, a major oral pathogen, in response to complex compounds extracted from Aralia continentalis and Arctii Semen using organic solvents. Transcriptome analysis (RNA-seq) revealed that both natural extracts commonly upregulated or downregulated the expression of various genes associated with different metabolic and physiological activities. Three genes (SMU_1584c, SMU_2133c, SMU_921), particularly SMU_921 (rcrR), known as a transcription activator of two sugar phosphotransferase systems (PTS) involved in sugar transport and biofilm formation, exhibited consistent high expression levels. Additionally, component analysis of the A. continentalis extract was performed to compare its effects on gene expression changes with the A. Semen extract, and two active compounds were identified through gas chromatography-mass spectrometry (GC-MS) analysis of the active fraction. The n-hexane fraction (ACEH) from the A. continentalis extract exhibited antibacterial specificity against S. mutans, leading to a significant reduction in the viable cell counts of Streptococcus sanguinis and Streptococcus gordonii among the tested multi-species bacterial communities. These findings suggest the broad-spectrum antibacterial activity of the A. continentalis extract and provide essential foundational data for the development of customized antimicrobial materials by elucidating the antibacterial mechanism of the identified active compounds.

• Journal of the Korean Geosynthetics Society
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• v.13 no.1
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• pp.71-83
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• 2014

Busan is located at the mouth of Nakdong River and if an earthquake occurs, it is very likely that the damage by the earthquake will be worse as liquefaction can happen in the sand layer, builtup soil, and landfill ground due to amplification in the lower sedimentary layer that is well developed in the river mouth. Therefore, this study first examined the possibility of liquefaction in the replaced sand layer under breakwater using 14 earthquakes in 5.6-7.9 scale and artificial earthquakes including the seismic wave suggested in the standard specifications for seismic design of ports and fishing port facilities to evaluate the stability of breakwater which is the primary protective structure for port facilities against earthquakes. Second, analysis on characteristics of the seismic energy and acceleration response spectrum by size of earthquake was performed to suggest the most appropriate size of seismic wave for the condition in Korea. Third, finite element analysis was performed using the suggested seismic wave to study the characteristics of earthquake by finding the dynamic lateral displacement of breakwater and verifying the stability of structure and the displacement and forces occurring at geotextile. Results of the study showed that the possibility of liquefaction in the landfill and replaced sand layer, the dynamic lateral displacement of breakwater, and changes of geotextile are greatly affected by the subsurface ground (replaced sand layer).

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.3
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• pp.207-214
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• 2017

High voltage electric power transmitter GIS(Gas Insulated Switchgear) above 72.5kV needs to satisfy domestic Korean peninsular standard(ES-6110-0002) in KEPCO with respect to normal and special operation conditions which include internal gas pressure, dead weight, wind and seismic load. Some other requirements not described in Korean standard can be applied from other international standards such as IEC(International Electronical Committee) 62271-203 and 62271-207. The GIS is a kind of pressure vessel structure made of aluminum and filled with SF6 gas of internal pressure 0.4~0.5MPa. Finite element analysis of GIS is performed with such operational loads including seismic loading and the stability and reliability is determined according to ASME BPVC(Boiler and Pressure Vessel Code) SEC. VIII standard where the allowable stress level of the pressure vessel is suggested. The result shows that the stress of GIS is satisfied the allowable stress level and the safety factor is about 2.3 for Korean peninsular standard.

• Journal of the Korea Concrete Institute
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• v.26 no.3
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• pp.351-360
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• 2014

The purpose of this research is to introduce the simplified equivalent frame model for the equivalent lateral force procedure, the response spectrum procedure and nonlinear procedure according to ASCE7-10 in order to reduce the time of performance and reasonably evaluate the effect of applying the damping system with the various conditions for the analysis and the variable. In this research, the seismic performance assessment and the design of the damping system were conducted through the nonlinear time history analysis based on the performance based seismic design in ASCE7-10 in regard to applying the damping system to apartment buildings which is lately issued. The optimal design based on the 75% of seismic base shear was performed for an apartment building. The seismic performance assessment were conducted to check the safety of the building, and the economic evaluation was performed by comparing the amount of resource for the optimal designed building with the amount of resource for the original building. In addition, hysteresis dampers was applied to the apartment building, and the suggested equivalent frame model was performed using the damping system design in ASCE7-10, then its control effects were proved in the full scale model of the apartment building which was used in this research.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.1
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• pp.141-152
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• 2015

The objective of this study is to estimate shear wave velocity of rockfill materials by making practical use of the micro-earthquake records which are ordinarily obtained at a domestic rockfill dam and to verify its applicability. Micro-earthquake records were obtained at the site of Heongseong dam and Soyanggang dam, which are the existing multi-purpose dams in Korea. In the previous study, the fundamental periods of each dam were already evaluated by analyzing the response spectrum of the observed records. In this study, numerical analyses varying shear moduli of rockfill zone were carried out using the acceleration histories measured at the abutment as input ground motions. From comparison between the fundamental periods calculated by numerical analyses and measured records, the shear wave velocities with depth were estimated. It is found that the effect of different earthquake events on shear wave velocity estimation for the target dam materials is negligible and the shear wave velocity can be consistently evaluated. Furthermore, comparing the shear wave velocity with the previous researchers' empirical relationships and field test results, applicability of suggested method is verified. Therefore, in case that it is impossible to conduct field tests and estimation is preliminary, the suggested method can be practically used.

• Journal of Ginseng Research
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• v.41 no.1
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• pp.43-51
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• 2017

Background: BIOGF1K, a compound K-rich fraction prepared from the root of Panax ginseng, is widely used for cosmetic purposes in Korea. We investigated the functional mechanisms of the anti-inflammatory and antioxidative activities of BIOGF1K by discovering target enzymes through various molecular studies. Methods: We explored the inhibitory mechanisms of BIOGF1K using lipopolysaccharide-mediated inflammatory responses, reporter gene assays involving overexpression of toll-like receptor adaptor molecules, and immunoblotting analysis. We used the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to measure the antioxidative activity. We cotransfected adaptor molecules, including the myeloid differentiation primary response gene 88 (MyD88) and Toll/interleukin-receptor domain containing adaptor molecule-inducing interferon-${\beta}$ (TRIF), to measure the activation of nuclear factor (NF)-${\kappa}B$ and interferon regulatory factor 3 (IRF3). Results: BIOGF1K suppressed lipopolysaccharide-triggered NO release in macrophages as well as DPPH-induced electron-donating activity. It also blocked lipopolysaccharide-induced mRNA levels of interferon-${\beta}$ and inducible nitric oxide synthase. Moreover, BIOGF1K diminished the translocation and activation of IRF3 and NF-${\kappa}B$ (p50 and p65). This extract inhibited the upregulation of NF-${\kappa}B$-linked luciferase activity provoked by phorbal-12-myristate-13 acetate as well as MyD88, TRIF, and inhibitor of ${\kappa}B$ ($I{\kappa}B{\alpha}$) kinase ($IKK{\beta}$), and IRF3-mediated luciferase activity induced by TRIF and TANK-binding kinase 1 (TBK1). Finally, BIOGF1K downregulated the NF-${\kappa}B$ pathway by blocking $IKK{\beta}$ and the IRF3 pathway by inhibiting TBK1, according to reporter gene assays, immunoblotting analysis, and an AKT/$IKK{\beta}$/TBK1 overexpression strategy. Conclusion: Overall, our data suggest that the suppression of $IKK{\beta}$ and TBK1, which mediate transcriptional regulation of NF-${\kappa}B$ and IRF3, respectively, may contribute to the broad-spectrum inhibitory activity of BIOGF1K.

• Journal of Korea Entertainment Industry Association
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• v.13 no.4
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• pp.93-106
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• 2019

Persona is an actor's external ego constructed by playing various roles, and his/her another self-portrait in the eyes of the audience. This study was conducted to analyze persona identity containing core persona(CP) and to gain implications for the growth strategy of the actress Son Ye-jin called "melo queen" by verifying consistency between the CP and audience responses to her starring works of the past. According to the related theories and models, the persona was firstly set as image, visuality, personality and consistency, and it was used to extract and sort descriptive texts about Son related news articles in the last 5 years of the six major Korean newspapers using the content analysis method. After that, we analyzed the number of viewers of her movies and the audience share of her dramas by genre. As a result, Son's persona components were found to have a proportion for 54.2% images (34.0% for melo and romance images, 20.2% for non-melo and romance images), 25.6% for visibility, 13.8% for consistency, and 6.4% for personality. Her CP was derived from a melo and romance image. Comparing this with the audience reaction, the melo romance genre dominated and showed consistency in the drama, but in the case of the film, the non-melo romance was dominant and did not match each other. The results were attributed to a wide gap between dramas and movies in terms of key viewers, box office factors, degree of genre hybridity and experimentality. Therefore, Son should actively use her CP in the drama and challenge the various roles in order to expand her persona spectrum in the film.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.3
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• pp.71-79
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• 2023

It is not efficient to install a maintenance system that measures seismic acceleration and displacement on all bridges and buildings to evaluate the safety of structures after an earthquake occurs. In order to maintain this, an on-site investigation is conducted. Therefore, it takes a lot of time when the scope of the investigation is wide. As a result, secondary damage may occur, so it is necessary to predict the safety of individual structures quickly. The method of estimating earthquake damage of a structure includes a finite element analysis method using approved seismic information and a structural analysis model. Therefore, it is necessary to predict the seismic information generated at arbitrary location in order to quickly determine structure damage. In this study, methods to predict the ground response spectrum and acceleration time history at arbitrary location using linear estimation methods, and artificial neural network learning methods based on seismic observation data were proposed and their applicability was evaluated. In the case of the linear estimation method, the error was small when the locations of nearby observatories were gathered, but the error increased significantly when it was spread. In the case of the artificial neural network learning method, it could be estimated with a lower level of error under the same conditions.

• Tuberculosis and Respiratory Diseases
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• v.48 no.5
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• pp.720-729
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• 2000

Background : Histological analysis of tuberculosis shows a spectrum of findings, from well formed granulomatous inflammation with few bacilli in patient with normal immune response to M. tuberculosis to poorly formed granulomatous inflammation with many bacilli in patient with defective immune response. To evaluate the degree of immune response to M. tuberculosis, we studied the histologic features, including the presence of acid fast bacilli(AFB) in lymph node of patients with cervical tuberculous lymphadenitis, and compared them with clinical characteristics. Methods : We reviewed the histologic features of 33 cases of cervical tuberculous lymphadenitis and processed the excised nodes for auramine-rhodamine staining to detect AFB. The AFB positivity in tissue was compared with the histologic features(degree of granuloma formation, presence of caseation necrosis, presence of neutrophilic infiltration) and clinical characteristics(lymph node size, duration of symptom, presence of local symptom or radiologic evidence of pulmonary tuberculosis). Results : 1) The mean age at diagnosis was 42.4 years, and male to female ratio was 1:4.5. 2) Histologically, all cases showed well formed granuloma and variable degrees of caseation necrosis, and 39% of the cases showed neutrophilic infiltration in the granulomatous inflammation. 3) AFB were confirmed in 52% of the cases, and they were found extracellularly and at the periphery of caseation necrosis. 4) There was no association between AFB positivity and histological features or clinical characteristics. Conclusion : Cervical tuberculous lymphadenitis showed well formed granulomatous inflammation with caseation necrosis, and there was no association between AFB positivity in the tissue and histological or clinical characteristics.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.24 no.3
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• pp.307-317
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• 2011

This paper is to investigate the possibility of the elastic seismic design for highrise buildings through seismic performance evaluation for potential earthquakes that wind-designed highrise buildings located in strong wind zone and low seismicity can be experienced. Highrise steel diagrid frames which is the most loved structural system in recent years were wind-designed and the substantial system overstrength due to wind design procedure is verified, For the highrise steel diagrid frames, the response spectrum analysis and the seismic performance evaluation by various soil sites were conducted. It was showed that highrise steel diagrid frames with slenderness of greater than 5.2 under strong wind and low seismic zones such as Korea peninsula can resist elastically for the 500 year return period earthquake and have the possibility of seismic design for the 2400 year return period earthquake. In the member level, highrise steel diagrid frames with slenderness of greater than 5.2 all presented the immediate occupancy level regardless of soil sites for the 500 year return earthquake and excluding the $S_E$ soil site for the even 2400 year return period earthquake. In the system level, highrise steel diagrid frames with slenderness of greater than 5.2 showed the immediate occupancy level for $S_A$ and $S_B$ soil sites and the life safety for $S_C$ to $S_E$ soil site in the 500 year return period. The seismic performance level of highrise steel diagrid frames for the 2400 year return period earthquake displayed one step lower than the 500 year return period earthquake.