• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.89-95
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• 2006

Potato plants carrying the Ry gene are extremely resistance to a number of potyviruses, but it is not known which variety expressed the resistance. In this investigation, combined classical and molecular techniques were used to identify virus resistance potatoes. Mechanical inoculation of 32 varieties of Korean potato cultivars, with potato virus Y (PVY), induced various symptoms, such as mosaic, yellowing, necrosis, mottle, vein clearing and vein bending. Different virus spreading patterns were observed, such as highly sensitive, moderate and resistant to $PVY^o$ inoculated leaves in different cultivars. From the results of double antibody sandwich-enzyme links immunosorbant assays (DAS-ELISA), coupled with reverse transcription polymerase chain reaction (RT-PCR), Winter valley and Golden valley were found to be highly susceptible and resistant cultivars to $PVY^o$ respectively. TEM was used as a complementary method to conform the localization of the virus in leaf tissues. TEM detect virus particles in Golden valley, where, ELISA and RT-PCR were unable to detect the CP gene. However, the interior part of the tissues was severely deformed in $PVY^o$ infected Winter valley, than Golden valley The Ry gene is involved in an induced response in $PVY^o$ infected Golden valley plants. The methods described in this study could be applied for the screening and development of antiviral potatoes.

• 시큐리티연구
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• 제3호
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• pp.83-116
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• 2000

Many concerns have been raised for importance of disaster management after 1990's numerous urban disasters in Korea. When the primary function of government is to protect lives and property of citizens, disaster management should be included in the mainstream of public administration and many hazard countermeasures should be carried out for that purpose. The principal purpose of this research is to establish ways and means needed to improve the disaster management system in Korea. This paper is split into five chapters. Chapter I is the introduction part. Chapter II introduces the reader to a disaster management theory, and deals with various disaster management systems of foreign countries such as the U.S.A. and Japan, Chapter III describes and characterizes the Korea's current disaster management system. The Korean disaster management system has been reestablished through the enactment of the Disaster Management Act of 1995 and the full revision of Natural Hazards Management Act of 1995 after series of catastrophic man-made disasters since 1993. Chapter IV is devoted to the establishment of an effective disaster management system in Korea. In this chapter, I discusses measures needed to improve the quality of CDMB(Central Disaster Management Bureau)'s service to the people. I strongly reemphasize the effectiveness of a comprehensive risk-based, all-hazard national emergency management system that addresses preparedness, mitigation, response, and recovery. To that end the Administration should develop legislation, new policies, and organizational plans to invigorate the agency with a refocussed mission. Chapter V is a conclusion. As the demand for citizens'welfare has been continuously increased in the localization, the importance of disaster management is emerging subject in the public sector. The disaster management carries out complicated affairs and manages diverse objects, the cooperation/connection system of the central-local government and citizens, and non-governmental organization should be established.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.524-528
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• 2017

Ricinus communis, belongs to the family Euphorbiaceae, has been known as medicinal plants for treatment of inflammation, tumors, antidiabetic, hepatoprotective and laxative. Compared to many pharmacological studies, the effect of R. communis extract on regulating adipogenesis as therapeutic drug for treating obesity has not been reported. R. communis extract (RCE) was investigated to determine its effects on the adipogenesis by monitoring the status of $Wnt/{\beta}-catenin$ signaling and factors involving the differentiation of adipocytes. The differentiation of 3T3-L1 cells monitored by Oil Red O staining was inhibited in concentration dependent manner by RCE. The luciferase activity of HEK 293-TOP cells containing pTOPFlash with Tcf4 response element-luciferase gene was increased approximately 2-folds by the treatment of RCE at concentrations of $100{\mu}g/mL$ compared to the control. Activation of the $Wnt/{\beta}-catenin$ pathway by RCE was further confirmed by immunocytochemical analysis which shows an increment of nuclear localization of ${\beta}-catenin$. In addition, safety of RCE was verified through performing neural stem cell morphology assay. Among the identified flavonoids in RCE, isoquercitrin was the most abundant. Therefore, these results indicate that the adipocyte differentiation was significantly reduced by isoquercitrin in R. communis. In this study, RCE suppresses the adipogenesis of 3T3-L1 cells via the activation of $Wnt/{\beta}-catenin$ signaling.

• Molecules and Cells
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• 제39권4호
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• pp.299-309
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• 2016

We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

• 한국통신학회논문지
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• 제41권2호
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• pp.178-188
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• 2016

스마트폰의 증가와 PC 성능 향상으로 유무선 통신망에 트래픽이 폭발적으로 증가하고 있다. 여기에는 페이스북, 유투브와 같은 멀티미디어 서비스와 파일 공유가 큰 부분을 차지하고 있다. CDN(Content Delivery Network)은 원거리에 있는 콘텐츠 사업자의 웹 서버에 저장된 콘텐츠를 이용자 근처 CDN 서버에 미리 저장, 콘텐츠 요구 발생 시 최적의 CDN 서버로부터 콘텐츠를 제공하는 콘텐츠 전송 기술이다. 본 논문에서는 콘텐츠 요청 메시지 전달에 Minimum Spanning Tree(MST) 알고리즘을 응용한 SCRP(Shortest Core Routing Path) 알고리즘을 사용해 CDN 서버와 클라이언트의 콘텐츠 전달에 이용되는 전체 트래픽 양을 최적화하였다. 또한 HC_LRU 캐시 알고리즘을 통해 캐시 적중률을 향상시킴으로써 콘텐츠 요청에 대한 평균 응답시간을 단축시켰다. 제안한 SCRP와 HC_LRU 알고리즘을 통해 트래픽을 지역화하고 병목현상을 방지하여 네트워크 자원을 효율적으로 사용하는 확장성 있는 콘텐츠 전송 네트워크 시스템을 구축할 수 있다.

• 한국구조물진단유지관리공학회 논문집
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• 제19권5호
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• pp.30-37
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• 2015

본 연구에서는 웨이블릿 변환을 적용한 시스템 감쇠비 평가에 있어서 고유주파수가 저주파 영역에 속하고, 비교적 높은 감쇠비를 갖는 응답신호에 대하여 웨이블릿 기저함수의 중심주파수 영향을 분석하고자 하였다. 이를 위하여 단일 모드로 구성된 신호와 일정 주파수를 이격시킨 분리 중첩 모드 신호 및 모드 주파수 성분을 근접시킨 인접 중첩 모드 신호에 대하여 수치해석으로 분석하고, H-Beam을 통한 실내실험을 수행하였다. 분석하고자 하는 모드의 고유주파수는 전체 스케일에 대한 대응 스케일로서 고려되고, 이러한 대응 스케일의 위치는 웨이블릿 기저함수의 중심주파수에 영향을 받게 된다. 따라서 각 모드의 고유주파수에 대응되는 스케일이 전체 스케일의 1/2에 위치되도록 웨이블릿 기저함수의 중심주파수가 선택될 때 감쇠비 평가에 대한 신뢰성이 향상 될 것이다.

• Smart Structures and Systems
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• 제23권6호
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• pp.653-667
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• 2019

Using cross-ties to connect cables together when forming a cable network is regarded as an efficient method of mitigating cable vibrations. Cross-ties have been extended and fixed on bridge decks or towers in some engineering applications. However, the dynamics of this kind of system need to be further studied, and the effects of extending cross-links to bridge decks/towers on the modal response of the system should be assessed in detail. In this paper, a system of two cables connected by an inter-supported cross-link with another lower cross-link extended to the ground is proposed and analyzed. The characteristic equation of the system is derived, and some limiting solutions in closed form of the system are derived. Roots of cable system with special configurations are also discussed, attention being given to the case when the two cables are identical. A predictable mode behavior was found when the stiffness of inter-connection cross-link and the cross-link extended to the ground were the same. The vector of mode energy distribution and the degree of mode localization index are proposed so as to distinguish global and local modes. The change of mode behaviors is further discussed in the case when the two cables are not identical. Effects of cross-link stiffness, cross-link location, mass-tension ratio, cable length ratio and frequency ratio on $1^{st}$ mode frequency and mode shape are addressed.

• Molecules and Cells
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• 제42권2호
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• pp.135-142
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• 2019

OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies, especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the isoform of OCT4B, OCT4B-190 ($OCT4B^{19kDa}$) was highly expressed in human glioblastoma stem cells and glioblastoma cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of $OCT4B^{19kDa}$ promoted colony formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed OCT4B at low levels. Thus, $OCT4B^{19kDa}$ may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.

• Biomolecules & Therapeutics
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• 제27권3호
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• pp.302-310
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• 2019

Melanoma cells have been shown to respond to BRAF inhibitors; however, intrinsic and acquired resistance limits their clinical application. In this study, we performed RNA-Seq analysis with BRAF inhibitor-sensitive (A375P) and -resistant (A375P/Mdr with acquired resistance and SK-MEL-2 with intrinsic resistance) melanoma cell lines, to reveal the genes and pathways potentially involved in intrinsic and acquired resistance to BRAF inhibitors. A total of 546 differentially expressed genes (DEGs), including 239 up-regulated and 307 down-regulated genes, were identified in both intrinsic and acquired resistant cells. Gene ontology (GO) analysis revealed that the top 10 biological processes associated with these genes included angiogenesis, immune response, cell adhesion, antigen processing and presentation, extracellular matrix organization, osteoblast differentiation, collagen catabolic process, viral entry into host cell, cell migration, and positive regulation of protein kinase B signaling. In addition, using the PAN-THER GO classification system, we showed that the highest enriched GOs targeted by the 546 DEGs were responses to cellular processes (ontology: biological process), binding (ontology: molecular function), and cell subcellular localization (ontology: cellular component). Ingenuity pathway analysis (IPA) network analysis showed a network that was common to two BRAF inhibitorresistant cells. Taken together, the present study may provide a useful platform to further reveal biological processes associated with BRAF inhibitor resistance, and present areas for therapeutic tool development to overcome BRAF inhibitor resistance.

• 한국정보통신학회논문지
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• 제24권2호
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• pp.179-185
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• 2020

최근 지진조기경보시스템(EEWS)의 성능 개선과 진앙지 또는 진앙 근처 지역의 지진방재 효과를 보완하기 위해 현장 지진조기경보시스템(On-Site EEWS)의 개발이 시도되고 있다. 지진에 대한 연구 및 관측을 위한 지진관측망을 이용하여 지진 방재에 활용하는 국가 차원의 EEWS와 달리 On-Site EEWS는 지진방재를 목적으로 하므로 성능 및 비용 측면의 효율적인 설계와 평가가 필요하다. 현재 우리나라는 EEWS 뿐만 아니라 On-Site EEWS 개발에 기존 EEWS의 설계 기준 및 평가방법을 사용하는 등 필요한 핵심기술과 운영 노하우가 미흡한 실정이다. 본 연구에서는 지진·화산재해대책법에서 규정하는 지진 가속도계의 요구 사항을 기반으로 On-Site EEWS의 국산화 개발에 필요한 지진 데이터 수집, 데이터 처리 및 분석 부분의 하드웨어 및 소프트웨어적 설계 방향과 성능평가 항목 및 방법을 제안한다.