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The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells

  • Attaur Rahman;Yuhao Li;Nur Izzah Ismail;To-Kiu Chan;Yuzhen Li;Dachun Xu;Hao Zhou;Sang-Bing Ong
    • International Journal of Stem Cells
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    • v.16 no.2
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    • pp.123-134
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    • 2023
  • Objective: The heart contains a pool of c-kit+ progenitor cells which is believed to be able to regenerate. The differentiation of these progenitor cells is reliant on different physiological cues. Unraveling the underlying signals to direct differentiation of progenitor cells will be beneficial in controlling progenitor cell fate. In this regard, the role of the mitochondria in mediating cardiac progenitor cell fate remains unclear. Specifically, the association between changes in mitochondrial morphology with the differentiation status of c-kit+ CPCs remains elusive. In this study, we investigated the relationship between mitochondrial morphology and the differentiation status of c-kit+ progenitor cells. Methods and Results: c-kit+ CPCs were isolated from 2-month-old male wild-type FVB mice. To activate differentiation, CPCs were incubated in α-minimal essential medium containing 10 nM dexamethasone for up to 7 days. To inhibit Drp1-mediated mitochondrial fragmentation, either 10 μM or 50 μM mdivi-1 was administered once at Day 0 and again at Day 2 of differentiation. To inhibit calcineurin, either 1 μM or 5 μM ciclosporin-A (CsA) was administered once at Day 0 and again at Day 2 of differentiation. Dexamethasone-induced differentiation of c-kit+ progenitor cells is aligned with fragmentation of the mitochondria via a calcineurin-Drp1 pathway. Pharmacologically inhibiting mitochondrial fragmentation retains the undifferentiated state of the c-kit+ progenitor cells. Conclusions: The findings from this study provide an alternative view of the role of mitochondrial fusion-fission in the differentiation of cardiac progenitor cells and the potential of pharmacologically manipulating the mitochondria to direct progenitor cell fate.

Analysis of Customer Evaluations on the Ethical Response to Service Failures of Foodtech Serving Robots (푸드테크 서빙로봇의 서비스 실패에 대한 직업윤리적 대응에 대한 고객 평가 분석)

  • Han, Jeonghye;Choi, Younglim;Jeong, Sanghyun;Kim, Jong-Wook
    • Journal of Service Research and Studies
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    • v.14 no.1
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    • pp.1-12
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    • 2024
  • As the service robot market grows among the food technology industry, the quality of robot service that affects consumer behavioral intentions in the restaurant industry has become important. Serving robots, which are common in restaurants, reduce employee work through order and delivery, but because they do not respond to service failures, they increase customer dissatisfaction as well as increase employee work. In order to improve the quality of service beyond the simple function of receiving and serving orders, functions of recovery effort, fairness, empathy, responsiveness, and certainty of the process after service failure, such as serving employees, are also required. Accordingly, we assumed the type of failure of restaurant serving service as two internal and external factors, and developed a serving robot with a vocational ethics module to respond with a professional ethical attitude when the restaurant serving service fails. At this time, the expression and action of the serving robot were developed by adding a failure mode reflecting failure recovery efforts and empathy to the normal service mode. And by recruiting college students, we tested whether the service robot's response to two types of service failures had a significant effect on evaluating the robot. Participants responded that they were more uncomfortable with service failures caused by other customers' mistakes than robot mistakes, and that the serving robot's professional ethical empathy and response were appropriate. In addition, unlike the robot's favorability, the evaluation of the safety of the robot had a significant difference depending on whether or not a professional ethical empathy module was installed. A professional ethical empathy response module for natural service failure recovery using generative artificial intelligence should be developed and mounted, and the domestic serving robot industry and market are expected to grow more rapidly if the Korean serving robot certification system is introduced.

Sound Absorption Properties of Sound Absorption Materials Using Zelkova serrata Leaves

  • Eunji Bae;Junho Goh;Dahye Yeom;Kyungrok Won;Reekeun Kong;Heeseop Byeon
    • Journal of Forest and Environmental Science
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    • v.40 no.2
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    • pp.90-98
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    • 2024
  • This study analyzes the characteristics of sound-absorbing materials made from forest by-products of the deciduous tree species Zelkova serrata (Z. serrata) by evaluating their sound absorption performance. Accordingly, sound-absorbing materials with varying sample thicknesses, leaf sizes, and drying conditions were fabricated. The sound absorption properties were measured using the impedance tube method via middle-type measurement tube (100 Hz-3,200 Hz). The sound absorption properties were evaluated using the average sound absorption coefficient (ASAC), which was calculated from the measured sound absorption coefficients at 250 Hz, 500 Hz, 1,000 Hz, and 2,000 Hz. The ASAC value significantly improved as the leaf size increased to 0.5×0.5 cm2, 1.0×1.0 cm2, and 2.0×2.0 cm2. The ASAC values under the two drying conditions were similar. There was no significant difference in ASAC according to the leaf size under the air-dried leaf condition, with a thickness of 2.50 cm. The highest ASAC value according to the sound-absorbing material thickness was 0.47 at a thickness of 2.50 cm and leaf size of 2.0×2.0 cm2 under the air-dried leaf condition. In addition, the variation in ASAC was 0.23, indicating that the sound absorption performance according to leaf thickness was more significant than the difference in absorption properties according to leaf size. A sound absorption coefficient (SAC) of 0.4 or higher was observed across the measurable frequency band (100 Hz-3,200 Hz). Furthermore, the SAC values with respect to leaf size and thickness were close to 1 in the high-frequency range above 2,000 Hz. Therefore, it is considered that sound-absorbing materials using Z. serrata leaves are advantageous in the field of absorbing noise in a high-frequency band of 2,000 Hz or more, and it is better to manufacture a thickness of 2.50 and 2.0×2.0 cm2.

Studying the Comparative Analysis of Highway Traffic Accident Severity Using the Random Forest Method. (Random Forest를 활용한 고속도로 교통사고 심각도 비교분석에 관한 연구)

  • Sun-min Lee;Byoung-Jo Yoon;WutYeeLwin
    • Journal of the Society of Disaster Information
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    • v.20 no.1
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    • pp.156-168
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    • 2024
  • Purpose: The trend of highway traffic accidents shows a repeating pattern of increase and decrease, with the fatality rate being highest on highways among all road types. Therefore, there is a need to establish improvement measures that reflect the situation within the country. Method: We conducted accident severity analysis using Random Forest on data from accidents occurring on 10 specific routes with high accident rates among national highways from 2019 to 2021. Factors influencing accident severity were identified. Result: The analysis, conducted using the SHAP package to determine the top 10 variable importance, revealed that among highway traffic accidents, the variables with a significant impact on accident severity are the age of the perpetrator being between 20 and less than 39 years, the time period being daytime (06:00-18:00), occurrence on weekends (Sat-Sun), seasons being summer and winter, violation of traffic regulations (failure to comply with safe driving), road type being a tunnel, geometric structure having a high number of lanes and a high speed limit. We identified a total of 10 independent variables that showed a positive correlation with highway traffic accident severity. Conclusion: As accidents on highways occur due to the complex interaction of various factors, predicting accidents poses significant challenges. However, utilizing the results obtained from this study, there is a need for in-depth analysis of the factors influencing the severity of highway traffic accidents. Efforts should be made to establish efficient and rational response measures based on the findings of this research.

Factors Influencing Effects of Korea's Rural Life Improvement Program on Quality of Life of Rural Women (한국의 농촌 생활개선사업이 농촌여성의 삶의 질에 미치는 영향요인)

  • Bereket Roba Gamo;Yoon-Ji Choi;Jung-Shin Choi;Joo-Lee Son
    • Journal of Agricultural Extension & Community Development
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    • v.30 no.4
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    • pp.243-257
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    • 2023
  • Rural life improvement programs (RLIPs) have been implemented with a central goal of improving the quality of rural life and promoting rural welfare and cultural life. However, different factors may influence the effect of rural life improvement programs on rural communities or households. This study aimed to investigate the determinants of perceived effects of RLIPs on quality of life of rural women in South Korea. We used a mixed research design to generate data for this study. We collected survey data from 311 rural women who participated in the RLIPs and also conducted a focus group discussion. We analyzed the quantitative data using descriptive statistics and hierarchical regression to identify the variables that predicted effects of RLIPs on quality of life of rural women. Our study finds that age, type of residence in the community, leadership experience, level of education, community satisfaction and community participation influenced respondents' perceived effects of RLIPs. The results imply that the benefits of a development intervention could not be uniformly reaped by residents of a community.

Digital Library Interface Research Based on EEG, Eye-Tracking, and Artificial Intelligence Technologies: Focusing on the Utilization of Implicit Relevance Feedback (뇌파, 시선추적 및 인공지능 기술에 기반한 디지털 도서관 인터페이스 연구: 암묵적 적합성 피드백 활용을 중심으로)

  • Hyun-Hee Kim;Yong-Ho Kim
    • Journal of the Korean Society for information Management
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    • v.41 no.1
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    • pp.261-282
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    • 2024
  • This study proposed and evaluated electroencephalography (EEG)-based and eye-tracking-based methods to determine relevance by utilizing users' implicit relevance feedback while navigating content in a digital library. For this, EEG/eye-tracking experiments were conducted on 32 participants using video, image, and text data. To assess the usefulness of the proposed methods, deep learning-based artificial intelligence (AI) techniques were used as a competitive benchmark. The evaluation results showed that EEG component-based methods (av_P600 and f_P3b components) demonstrated high classification accuracy in selecting relevant videos and images (faces/emotions). In contrast, AI-based methods, specifically object recognition and natural language processing, showed high classification accuracy for selecting images (objects) and texts (newspaper articles). Finally, guidelines for implementing a digital library interface based on EEG, eye-tracking, and artificial intelligence technologies have been proposed. Specifically, a system model based on implicit relevance feedback has been presented. Moreover, to enhance classification accuracy, methods suitable for each media type have been suggested, including EEG-based, eye-tracking-based, and AI-based approaches.

A method for localization of multiple drones using the acoustic characteristic of the quadcopter (쿼드콥터의 음향 특성을 활용한 다수의 드론 위치 추정법)

  • In-Jee Jung;Wan-Ho Cho;Jeong-Guon Ih
    • The Journal of the Acoustical Society of Korea
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    • v.43 no.3
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    • pp.351-360
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    • 2024
  • With the increasing use of drone technology, the Unmanned Aerial Vehicle (UAV) is now being utilized in various fields. However, this increased use of drones has resulted in various issues. Due to its small size, the drone is difficult to detect with radar or optical equipment, so acoustical tracking methods have been recently applied. In this paper, a method of localization of multiple drones using the acoustic characteristics of the quadcopter drone is suggested. Because the acoustic characteristics induced by each rotor are differentiated depending on the type of drone and its movement state, the sound source of the drone can be reconstructed by spatially clustering the results of the estimated positions of the blade passing frequency and its harmonic sound source. The reconstructed sound sources are utilized to finally determine the location of multiple-drone sound sources by applying the source localization algorithm. An experiment is conducted to analyze the acoustic characteristics of the test quadcopter drones, and the simulations for three different types of drones are conducted to localize the multiple drones based on the measured acoustic signals. The test result shows that the location of multiple drones can be estimated by utilizing the acoustic characteristics of the drone. Also, one can see that the clarity of the separated drone sound source and the source localization algorithm affect the accuracy of the localization for multiple-drone sound sources.

Exploring automatic scoring of mathematical descriptive assessment using prompt engineering with the GPT-4 model: Focused on permutations and combinations (프롬프트 엔지니어링을 통한 GPT-4 모델의 수학 서술형 평가 자동 채점 탐색: 순열과 조합을 중심으로)

  • Byoungchul Shin;Junsu Lee;Yunjoo Yoo
    • The Mathematical Education
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    • v.63 no.2
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    • pp.187-207
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    • 2024
  • In this study, we explored the feasibility of automatically scoring descriptive assessment items using GPT-4 based ChatGPT by comparing and analyzing the scoring results between teachers and GPT-4 based ChatGPT. For this purpose, three descriptive items from the permutation and combination unit for first-year high school students were selected from the KICE (Korea Institute for Curriculum and Evaluation) website. Items 1 and 2 had only one problem-solving strategy, while Item 3 had more than two strategies. Two teachers, each with over eight years of educational experience, graded answers from 204 students and compared these with the results from GPT-4 based ChatGPT. Various techniques such as Few-Shot-CoT, SC, structured, and Iteratively prompts were utilized to construct prompts for scoring, which were then inputted into GPT-4 based ChatGPT for scoring. The scoring results for Items 1 and 2 showed a strong correlation between the teachers' and GPT-4's scoring. For Item 3, which involved multiple problem-solving strategies, the student answers were first classified according to their strategies using prompts inputted into GPT-4 based ChatGPT. Following this classification, scoring prompts tailored to each type were applied and inputted into GPT-4 based ChatGPT for scoring, and these results also showed a strong correlation with the teachers' scoring. Through this, the potential for GPT-4 models utilizing prompt engineering to assist in teachers' scoring was confirmed, and the limitations of this study and directions for future research were presented.

A Study on Comparison of Vocational Aptitude For MBTI and Mingriology (MBTI와 명리학의 직업적성론 비교연구)

  • Min-Suk Kim;Sung-Tae Ryu
    • Industry Promotion Research
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    • v.9 no.3
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    • pp.213-227
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    • 2024
  • The MBTI personality type test is the most widely used standardized test as an occupational aptitude test and psychological test to identify a job suitable for an individual's aptitude. However, such a self-report test has many constraints on the subject's judgment, cognitive ability, situation, and time, so there is a limit to the test. Therefore, there is a need for a test tool that can compensate for the limitations of Western psychological tests, and Myeongnology can solve the shortcomings of Western occupational tests. This is because Myeong-Ri can distinguish a natural person's disposition, aptitude, and what kind of job he or she should engage in, and it is also possible to predict what kind of job he or she will change to according to good luck. Through a comparative study of the occupational sex theory between MBTI and Myeong-ri, it can be seen that the MBTI variables and the variables of Myeong-ri are quite consistent with the occupational sex. Therefore, it was proved that Myeong-Ri is a study that can be used for professional sex counseling while supplementing the limitations of MBTI. Therefore, it is expected that this paper will contribute to the study of occupational characteristics.

A Study on the Effect of Catchphrases in Corporate Job Postings on Job Applications from Individuals in Their 20s and 30s (기업 채용공고의 캐치프레이즈가 20, 30대의 입사 지원에 미치는 영향 분석)

  • Min Jae Hwang;Seung In Kim
    • Industry Promotion Research
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    • v.9 no.3
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    • pp.205-212
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    • 2024
  • This study aims to analyze the impact of catchphrases used in job postings on job applications in the context of accelerating social participation of Generation Z and changing business environments. To this end, case studies of catchphrases used in job postings by domestic and international companies were conducted. In addition, job postings for the Korea Electric Power Corporation (KEPCO), a well-known public institution, were created using four trend keywords: 'general phrases,' 'eco-friendliness,' 'employee welfare,' and 'social issues (equality).' An online survey was conducted to analyze the impact of catchphrases in job postings on job applications, and the results showed that 83% of respondents found that the catchphrases in job postings had a positive effect on their application decisions. Through in-depth interviews, the importance of design elements and catchphrases in job postings as perceived by actual job seekers was confirmed. In conclusion, this study aims to explore how catchphrases in job postings can facilitate smooth communication between companies and job seekers and strengthen corporate image promotion activities. It seeks to provide insights into how companies can effectively utilize catchphrases in job postings according to age and job type in future recruitment processes.