• Title/Summary/Keyword: Research laboratory

Search Result 16,341, Processing Time 0.042 seconds

Literature Review on Recent Magnetocardiography and Impedance-Magnetocardiography Technologies

  • Kandori, A.;Miyashita, T.;Ogata, K.;Seki, Y.;Suzuki, D.;Tsukamoto, A.;Saito, K.;Yokosawa, K.;Tsukada, K.;Yamada, Satsuki;Watanabe, Shigeyuki;Horigome, Hitoshi;Yamaguchi, Iwao
    • Progress in Superconductivity
    • /
    • v.8 no.1
    • /
    • pp.1-7
    • /
    • 2006

  • We have developed magnetocardiography(MCG) and impedance magnetocardiography(I-MCG) for detecting heart disease by using dc-SQUID technology. The MCG system, using low-Tc SQUID, is being applied commercially for diagnosing heart disease. Using the low-Tc MCG system, many clinical studies on detection of abnormality have been performed. Furthermore, we have developed a portable MCG system using high-Tc SQUID. For detecting changes in kinetic impedance in the heart, an I-MCG system has been demonstrated. The I-MCG system could detect the mechanical movement of the heart. In this report, we review current clinical applications of magnetocardiography and impedance magnetocardiography.

  • PDF

Inhibitory Activity against Protein Kinase C of Some Medicinal Plants (수종 생약의 Protein kinase C 저해활성)

  • Lee, Hyun-Sun;Ahn, Soon-Cheol;Kim, Bo-Hyun;Park, Moon-Su;Oh, Won-Keun;Yoon, Byung-Dae;Ahn, Jong-Seog;Mheen, Tae-Ick
    • Korean Journal of Pharmacognosy
    • /
    • v.23 no.3
    • /
    • pp.142-145
    • /
    • 1992

  • MeOH extract of twenty medicinal herbs were screened for their effects against protein kinase C (PKC) using bleb-forming assay and PKC enzyme assay. Smilax china and Sanguisorba officinalis showed potent anti-PKC activity. Campsis grandiflora and Galla Halepensis showed moderate inhibitory effect on PKC.

  • PDF

Sequence Analysis and Molecular Characterization of Wnt4 Gene in Metacestodes of Taenia solium

  • Hou, Junling;Luo, Xuenong;Wang, Shuai;Yin, Cai;Zhang, Shaohua;Zhu, Xueliang;Dou, Yongxi;Cai, Xuepeng
    • Parasites, Hosts and Diseases
    • /
    • v.52 no.2
    • /
    • pp.163-168
    • /
    • 2014

  • Wnt proteins are a family of secreted glycoproteins that are evolutionarily conserved and considered to be involved in extensive developmental processes in metazoan organisms. The characterization of wnt genes may improve understanding the parasite's development. In the present study, a wnt4 gene encoding 491amino acids was amplified from cDNA of metacestodes of Taenia solium using reverse transcription PCR (RT-PCR). Bioinformatics tools were used for sequence analysis. The conserved domain of the wnt gene family was predicted. The expression profile of Wnt4 was investigated using real-time PCR. Wnt4 expression was found to be dramatically increased in scolex evaginated cysticerci when compared to invaginated cysticerci. In situ hybridization showed that wnt4 gene was distributed in the posterior end of the worm along the primary body axis in evaginated cysticerci. These findings indicated that wnt4 may take part in the process of cysticerci evagination and play a role in scolex/bladder development of cysticerci of T. solium.

  • https://doi.org/10.3347/kjp.2014.52.2.163 인용 PDF KSCI

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus

  • Cao, Yuying;Yuan, Li;Yang, Shunli;Shang, Youjun;Yang, Bin;Jing, Zhizhong;Guo, Huichen;Yin, Shuanghui
    • Journal of Veterinary Science
    • /
    • v.23 no.5
    • /
    • pp.32.1-32.11
    • /
    • 2022

  • Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

  • https://doi.org/10.4142/jvs.21262 인용 PDF KSCI