• 제목/요약/키워드: Research laboratory

검색결과 16,341건 처리시간 0.039초

Differential Expression of Interferon-Tau Transcripts in Bovine Blastocysts Produced by In Vitro Fertilization and Somatic Cell Nuclear Transfer

  • Song, Bong-Suk;Koo, Deog-Bon;Gabbine Wee;Shim, Jung-Jae;Kim, Ji-Su;Lee, Kyung-Kwang;Han, Yong-Mahn
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.228-228
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    • 2004
  • Interferon-tau (IFN-τ) is the primary agent responsible for maternal recognition of pregnancy in cattle. Bovine embryos begine to express IFN-τ as the blastocyst forms. Pregnancy recognition in ruminants occurs when IFN-τ from the trophoblast prevents the increase of oxytocin receptors, disrupting luteolytic pulses of prostaglandin (PG) F2a by oxytocin. The expression of IFN-τ is strongly associated with the degree of methylation of the CpG islands in promoter region. (omitted)

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Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium

  • Choresca, Casiano H. Jr.;Gomez, Dennis K.;Han, Jee-Eun;Shin, Sang-Phil;Kim, Ji-Hyung;Jun, Jin-Woo;Park, Se-Chang
    • 대한수의학회지
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    • 제50권4호
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    • pp.331-333
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    • 2010
  • Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.

High-Quality Stereo Depth Map Generation Using Infrared Pattern Projection

  • Jeong, Jae-Chan;Shin, Hochul;Chang, Jiho;Lim, Eul-Gyun;Choi, Seung Min;Yoon, Kuk-Jin;Cho, Jae-Il
    • ETRI Journal
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    • 제35권6호
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    • pp.1011-1020
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    • 2013
  • In this paper, we present a method for obtaining a high-quality 3D depth. The advantages of active pattern projection and passive stereo matching are combined and a system is established. A diffractive optical element (DOE) is developed to project the active pattern. Cross guidance (CG) and auto guidance (AG) are proposed to perform the passive stereo matching in a stereo image in which a DOE pattern is projected. When obtaining the image, the CG emits a DOE pattern periodically and consecutively receives the original and pattern images. In addition, stereo matching is performed using these images. The AG projects the DOE pattern continuously. It conducts cost aggregation, and the image is restored through the process of removing the pattern from the pattern image. The ground truth is generated to estimate the optimal parameter among various stereo matching algorithms. Using the ground truth, the optimal parameter is estimated and the cost computation and aggregation algorithm are selected. The depth is calculated and bad-pixel errors make up 4.45% of the non-occlusion area.

Measurement of the excited Xe atoms density of matastable state$(1S_5)$ under various binary gas mixtures(Ne-Xe) by Laser Absorption Spectroscopy.

  • Lee, Jun-Ho;OH, P.Y.;Moon, M.W.;Ko, B.D.;Jeong, J.M.;Lee, H.J.;Choi, E.H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.II
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    • pp.1218-1220
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    • 2005
  • We have developed laser absorption spectroscopy system for the measurement of excited Xe atoms in micro-discharged AC-PDP plasma. In this study, we have measured the absorption signals for the $1S_5$ xenon metastable state in the PDP cell with the various gas mixtures of Ne-Xe(1%), Ne-Xe(4%) and Ne-Xe(10%) under fixed gas pressure of 350 Torr and the eletrode gap distance of 50um. It is found that the maximum excited xenon densities are $1.2^{\ast}10^{12}\;cm^{-3}$, $1.8^{\ast}10^{12}\;cm^{-3}$ and $2.7^{\ast}10^{12}cm^{-3}$ for gas mixtures of Ne-Xe(1%), Ne-Xe(4%) and Ne-Xe(10%) respectively, in this experiment.

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Cloning and Characterization of the Mycobacterium bovis BCG panB Gene Encoding Ketopantoate Hydroxymethyltransferase

  • Kim, Jin-Koo;Kim, Kwang-Dong;Lim, Jong-Seok;Lee, Hee-Gu;Kim, Sang-Jae;Cho, Sang-Hyun;Jeong, Won-Hwa;Choe, In-Seong;Chung, Thi-Wha;Paik, Sang-Gi;Choe, Yong-Kyung
    • BMB Reports
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    • 제34권4호
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    • pp.342-346
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    • 2001
  • The Mycobacterium bovis BCG panB gene, encoding ketopantoate hydroxymethyltransferase (KPHMT), was cloned from a ${\lambda}gt11$ genomic library and sequenced. The DNA sequence encodes a protein that contains 281 amino acid residues (M, 29,337) with a high similarity to the KPHMTs. Subcloning of a 846 by open reading frame (ORF), but not a 735 by ORF, into the vector pUC19 led to complementation of the panB mutant of Escherichia coli. The BCG pang gene was overexpressed in E. coli and the KPHMT purified to homogeneity The recombinant protein was further confirmed by an enzymatic assay.

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On the Behavior of Suspended Sediment near a Silt Screen and the Screen Efficiency in a Microtidal Coastal Area

  • Jin, Jae-Youll;Song, Won-Oh;Park, Jin-Soon;Chae, Jang-Won;Kim, Sung-En;Jeong, Weon-Mu;Yum, Ki-Dai;Oh, Jae-Kyung
    • 한국해안해양공학회:학술대회논문집
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    • 한국해안해양공학회 2003년도 한국해안해양공학발표논문집
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    • pp.344-352
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    • 2003
  • Sediment plumes arising from various coastal works can cause detrimental effects on the coastal ecosystem in various manners. Although the most active countermeasure against the plumes is to restrict the works to specified time periods known as environmental windows (Reine et al., 1998), silt screens have been widely used for reducing the spreading of suspended sediments (SS) generated by coastal works. (omitted)

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강유전체 박막과 마이크로 가공 기술을 이용한 초전형 적외선 센서의 제작 (Pyroelectric Infrared Microsensors Made by Micromachining Technology)

  • 최준림;이돈희;남효진;조성문;이주행;김광영;김성태
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1994년도 하계학술대회 논문집 A
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    • pp.66-68
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    • 1994
  • 강유전체 박막과 마이크로 가공기술을 이용하여 초전형 적외선 센서를 제작하였다. 초전형 적외선 센서는 $Pb_{1-x}La_xTi_{1-x/4}O_3$ (x=0.05) (PLT) 강유전체 박막 커패시터를 RF 마그네트론 스퍼터링 방식으로 백금 전극이 증착된 MgO 기판상에 결정 성장시킨 구조를 갖고 있다. 스퍼터링된 PLT 바닥은 높은 c-축 결정 구조를 가지므로 센서로 사용하기 위한 poling 처리 과정이 필요하다. 이는 적외선 이미지 센서를 구현함에 있어서 수율 향상에 필수적인 요소이다. 마이크로 가공 기술을 사용하여 센서의 열용량을 극소화 함으로서 센서의 효율을 최대화하였다. 제작된 센서의 상부에 폴리이미드를 코팅하고 MgO 기판을 선택적으로 식각하여 코팅된 폴리이미드가 강유전체 박막 커패시터를 지지하고 있는 구조를 구현하였다. 이렇게 제작된 센서의 감도는 상온에서 $8.5{\times}10^8cm{\cdot}{\sqrt}{Hz/W}$로 측정되었으며 이는 마이크로 가공 기술을 사공하지 않은 경우보다 약 100 때의 감도 향상을 가져왔다.

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밀폐공간에서의 화재 위험성에 관한 연구 (A Study on fire risk in an enclosure room)

  • 김수영;김홍식;한동훈;이성룡;김주석;육근환;천성수
    • 한국화재소방학회:학술대회논문집
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    • 한국화재소방학회 2009년도 춘계학술논문발표회 논문집
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    • pp.449-453
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    • 2009
  • 기존 주택 및 고층건물화재에서 발생하는 화재 폭발 사고에 대해서는 많은 연구가 이루어지고 있으나, 이천 냉동창고 화재와 같이 많은 인명피해가 발생되는 작업환경에서의 화재 특히 밀폐공간에서의 화재 폭발 위험성 및 특성은 주택 및 고층건물화재에 비교하여 높은 폭발위험성 및 빠른 화염전파 특성을 보이나 이에 관한여 구체적인 연구는 미비한 실정이다. 본 연구는 밀폐공간 작업환경에서의 화재 폭발 위험성 특성 분석을 위하여 밀폐공간에서 작업 시 발생되어지는 가연성 가스의 확산 특성 분석 및 폭발 위험성 분석 방법에 관한 연구와 밀폐공간에서의 화염 확산 특성에 관한 연구논문 이다.

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Morphological and molecular analysis of indigenous Myanmar mango (Mangifera indica L.) landraces around Kyaukse district

  • Kyaing, May Sandar;Soe, April Nwet Yee;Myint, Moe Moe;Htway, Honey Thet Paing;Yi, Khin Pyone;Phyo, Seinn Sandar May;Hlaing, Nwe Nwe Soe
    • Journal of Plant Biotechnology
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    • 제46권2호
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    • pp.61-70
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    • 2019
  • There is vast genetic diversity of Myanmar Mangoes. This study mainly focused on indigenous thirteen different mango landraces cultivated in central area of Myanmar, Kyauk-se District and their fruit characteristics by 18 descriptors together with genetic relationship among them by 12 SSR markers. Based on the morpho-physical characters, a wide variation among accessions was found. Genetic characterization of thirteen mango genotypes resulted in the detection of 302 scorable polymorphic bands with an average of 4.33 alleles per locus and an average polymorphism information content (PIC) of 0.7. All the genotypes were grouped into two major clusters by UPGMA cluster analysis and a genetic similarity was observed in a range of 61 ~ 85%. This study may somehow contribute insights into the identification of regional mango diversity in Myanmar and would be useful for future mango breeding program.

Complete mitochondrial genome of freshwater goby Rhinogobius cliffordpopei (Perciformes, Gobiidae): genome characterization and phylogenetic analysis

  • Zhong, Liqiang;Wang, Minghua;Li, Daming;Tang, Shengkai;Zhang, Tongqing;Bian, Wenji;Chen, Xiaohui
    • Genes and Genomics
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    • 제40권11호
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    • pp.1137-1148
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    • 2018
  • Freshwater gobies Rhinogobius cliffordpopei and R. giurinus are invasive species with particular concern because they have become dominant and were fierce competitors in the invaded areas in Yunnan-Guizhou Plateau (southwest of China). Information about genetic characteristics of R. giurinus have been published, but there were still no relevant reports about R. cliffordpopei. In present study, the complete mitochondrial genome of R. cliffordpopei was determined, which was 16,511 bp in length with A+T content of 51.1%, consisting of 13 protein-coding genes, 22 tRNAs, 2 ribosomal RNAs, and a control region. The gene composition and the structural arrangement of the R. cliffordpopei complete mtDNA were identical to most of other teleosts. Phylogenetic analyses placed R. cliffordpopei in a well-supported monophyletic cluster with other Rhinogobius fish. But the phylogenetic relationship between genus Rhinogobius and Tridentiger remained to be resolved.