• 제목/요약/키워드: Research laboratory

검색결과 16,341건 처리시간 0.055초

Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1

  • Li, Wen Hui;Jia, Wan Zhong;Qu, Zi Gang;Xie, Zhi Zhou;Luo, Jian Xun;Yin, Hong;Sun, Xiao Lin;Blaga, Radu;Fu, Bao Quan
    • Parasites, Hosts and Diseases
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    • 제51권2호
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    • pp.197-201
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    • 2013
  • A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.

Corrosion fatigue crack growth behavior of 316LN stainless steel in high-temperature pressurized water

  • Zhang, Ziyu;Tan, Jibo;Wu, Xinqiang;Han, En-Hou;Ke, Wei
    • Nuclear Engineering and Technology
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    • 제53권9호
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    • pp.2977-2981
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    • 2021
  • Corrosion fatigue crack growth (FCG) behavior of 316LN stainless steel was investigated in high-temperature pressurized water at different temperatures, load ratios (R = Kmax/Kmin) and rise times (tR). The environmental assisted effect on FCG rate was observed when both the R and tR exceeded their critical values. The FCG rate showed a linear relation with stress intensity factor range (ΔK) in double logarithmic coordinate. The environmental assisted effect on FCG rate depended on the ΔK and quantitative relations were proposed. Possible mechanisms of environmental assisted FCG rate under different testing conditions are also discussed.

Characterization of Newcastle disease virus obtained from toco toucan

  • Li, Jiaxin;Ling, Mengmeng;Sun, Yixue;Di, Haiyang;Cong, Yulin;Yu, Haiying;Cong, Yanlong
    • Journal of Veterinary Science
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    • 제21권2호
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    • pp.19.1-19.10
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    • 2020
  • Given that the current Newcastle disease virus (NDV) infection in wild birds poses the threat to poultry, surveillance of Newcastle disease in captive wild birds was carried out in Jilin, China in 2018. Here, an NDV strain obtained from toco toucan was firstly characterized. The results showed that the F gene of the NDV isolate Toucan/China/3/2018 is classified as genotype II in class II. Sequence analysis of the F0 cleavage site was 113RQGR/L117, which supports the result of the intracerebral pathogenicity index assay indicating classification of the isolate as low-pathogenicity. Experimental infection demonstrated that Toucan/China/3/2018 can effectively replicate and transmit among chickens. To our knowledge, this is the first report on genetically and pathogenically characterizing NDV strain isolated from toucan, which enriches the epidemiological information of NDV in wild birds.

An Efficient Method for the Large-Scale Synthesis of Atorvastatin Calcium

  • Lee, Hong-Woo;Kim, Young-Min;Yoo, Choong-Leol;Kang, Sung-Kwon;Ahn, Soon-Kil
    • Biomolecules & Therapeutics
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    • 제16권1호
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    • pp.28-33
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    • 2008
  • Atorvastatin calcium salt (1) was obtained through the preparation of lactone compound (8) from 2-((4R,6R)-6-(2-(2-(4-fluorophenyl)-5-isopropyl-3-phenyl-4-(phenylcarbamoyl)-1H-pyrrol-1-yl)-ethyl)-2-phenyl-1,3,2-dioxaborinan-4-yl)acetic acid tert-butyl ester (9) by hydrolysis in basic condition. Efficient hydrolysis of boronate compound 9 aimed at the viable synthesis for commercial production and purification of Atorvastatin calcium is reported. Detail studies of evaluation procedure are also reported.