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Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid

  • Lu, Yan;Sun, Jia-Hui;Lu, Li-Li;Chen, Jia-Xu;Song, Peng;Ai, Lin;Cai, Yu-Chun;Li, Lan-Hua;Chen, Shao-Hong
    • Parasites, Hosts and Diseases
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    • v.59 no.6
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    • pp.615-623
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    • 2021
  • Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.

e-MITOCHONDRIA RESEARCH FOR SYSTEMS BIOLOGY AND PROTEOMICS

  • Joo, Hyun;Youm, Jae-Boum;Kim, Tae-Ho;Kim, Na-Ri;Park, Won-Sun;Kang, Sung-Hyun;Cuong, Dang Van;Kim, Hyoung-Kyu;Khoa, Tran Min;Thu, Vu Thi;Kim, Hyun-Ju;Moon, Hye-Jin;Lee, Hyun-Suk;Kim, Eui-Yong;Han, Jin
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.295-295
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    • 2005
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Proton Generation with 3-% Energy Conversion Efficiency (3% 에너지 변환효율을 가진 양성자 발생)

  • Choe, Il-U;Kim, Cheol-Min;Jeong, Tae-Mun;Yu, Tae-Jun;Seong, Jae-Hui;Lee, Seong-Gu;Hafz, N.;Bae, Gi-Hong;No, Yeong-Cheol;Go, Do-Gyeong;Lee, Jong-Min;Nishiuchi, M.;Daido, H.;Yogo, A.;Orimo, S.;Ogura, K.;Ma, J.;Sagisaka, A.;Mori, M.;Pirozhkov, A.S.;Kiriyama, H.;Bulanov, S.V.;Esirkepov, T.Zh.;Oishi, Y.;Nemoto, K.;Nagatomo, H.;Nagai, K.
    • Proceedings of the Optical Society of Korea Conference
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    • 2008.07a
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    • pp.155-156
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    • 2008
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