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Gain of a New Exon by a Lineage-Specific Alu Element-Integration Event in the BCS1L Gene during Primate Evolution

  • Park, Sang-Je;Kim, Young-Hyun;Lee, Sang-Rae;Choe, Se-Hee;Kim, Myung-Jin;Kim, Sun-Uk;Kim, Ji-Su;Sim, Bo-Woong;Song, Bong-Seok;Jeong, Kang-Jin;Jin, Yeung-Bae;Lee, Youngjeon;Park, Young-Ho;Park, Young Il;Huh, Jae-Won;Chang, Kyu-Tae
    • Molecules and Cells
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    • v.38 no.11
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    • pp.950-958
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    • 2015

  • BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in human genome. In present study, we conducted evolutionary analysis of AluYRa2-exonized transcript with various primate genomic DNAs and cDNAs from humans, rhesus monkeys, and crabeating monkeys. Remarkably, our results show that AluYRa2 element has only been integrated into genomes of Macaca species. This Macaca lineage-specific integration of AluYRa2 element led to exonization event in the first intron region of BCS1L gene by producing a conserved 3' splice site. Intriguingly, in rhesus and crabeating monkeys, more diverse transcript variants by alternative splicing (AS) events, including exon skipping and different 5' splice sites from humans, were identified. Alignment of amino acid sequences revealed that AluYRa2-exonized transcript has short N-terminal peptides. Therefore, AS events play a major role in the generation of various transcripts and proteins during primate evolution. In particular, lineage-specific integration of Alu elements and species-specific Alu-derived exonization events could be important sources of gene diversification in primates.

  • https://doi.org/10.14348/molcells.2015.0121 인용 PDF KSCI

Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX

  • Kim, Ji Won;Kim, Eun Young;Kim, Sun Young;Byun, Sang Kyung;Lee, Dasom;Oh, Kyoung-Jin;Kim, Won Kon;Han, Baek Soo;Chi, Seung-Wook;Lee, Sang Chul;Bae, Kwang-Hee
    • Molecules and Cells
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    • v.37 no.10
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    • pp.742-746
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    • 2014

  • The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.

  • https://doi.org/10.14348/molcells.2014.0208 인용 PDF KSCI