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http://dx.doi.org/10.14348/molcells.2014.0208

Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX  

Kim, Ji Won (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Kim, Eun Young (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Kim, Sun Young (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Byun, Sang Kyung (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Lee, Dasom (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Oh, Kyoung-Jin (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Kim, Won Kon (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Han, Baek Soo (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Chi, Seung-Wook (Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Sang Chul (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Bae, Kwang-Hee (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Molecules and Cells / v.37, no.10, 2014 , pp. 742-746 More about this Journal
Abstract
The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.
Keywords
aptamer; EpCAM; SELEX; stem cell marker;
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