• Proceedings of the Korean Aquaculture Society Conference
• /
• 2003.10a
• /
• pp.40-40
• /
• 2003

DNA content of the sperm of tetraploid mud loach (Misgurnus mizolepis) males and the ploidy status of progenies generated by crossing tetraploid males with diploid females are described. Reproductive performance of the induced adult tetraploid males ranged from sterility to fertility. Of 48 tetraploid males tested, 12 were sterile but the other 36 produced functional sperm. Of these 36, 26 produced haploid sperm, which on fertilizing the haploid eggs, generated diploid progenies. Seven tetraploid males were mosaics in their sperm, as indicated by the production of diploid, aneuploid and/or triploid offspring. Only 3 males produced diploid sperm rendering the production of all-triploid progenies. The DNA content of sperm of a tested tetraploid male was consistent throughout the 3 progeny tests, i.e. the haploid sperm-producing 4n males persisted to produce the haploid sperm only likewise the diploid sperm producing4n males consistently produced the diploid sperm only, when progeny testing was extended to 3 successive but alternate years. Hence, a careful and direct examination of the DNA profile of sperm from tetraploid males is a pre-requisite for reproductive containment of genetically modified fish.

• Clinical and Experimental Reproductive Medicine
• /
• v.13 no.2
• /
• pp.95-100
• /
• 1986

During the past decade great advance has been made in contraceptive technology. The development and subsequent modification of oral steroid contraceptive together with the improved design of other contraceptives have tremendously altered the contraceptive practise of complete throughout the world. At the present time one of most complex issue is the population explosion. In Korea the population growth rate is remarkably decreased from 3% in 1960s to 1.7% in 1984. Increasing proportion of women practising family planning and improved contraceptive methods have contributed on remarkable reduction of fertility rate. But still about half of married women not involved in family planning practise. One of the reasons why that so many women haven't participated in family planning should be undesirable side effects or inconvenience of currently available contraceptives. So we need more research to develop newer, safer and more effective contraceptive to solve the problems. According to recent report on the family planning program achievement in Korea, sterilization (male and female) is most popular, leading contraceptive method (330 cases/1000 fertile persons) and the I.U.D. (167.5/1000), condom (132.9/1000) and oral pill (61.1/1000) were followed by in the order of favorite choice of contraceptive (KIPH, Family planning evaluation report, 1984; KIPH Family plan Service Statistics, 1981-1984). In present paper the present status and knowledge of contraceptives will be summarized.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.7
• /
• pp.1071-1086
• /
• 2003

Reproductive biotechnologies continue to be developed for genetic improvement of both river and swamp buffalo. Although artificial insemination using frozen semen emerged some decades back, there are still considerable limitations. The major problem appears to be the lack of efficient methods for estrus detection and timely insemination. Controlled breeding experiments in the buffalo had been limited and similar to those applied in cattle. Studies on multiple ovulation and embryo transfer are essentially a replica of those in cattle, however with inherent problems such as lower number of primordial follicles on the buffalo ovary, poor fertility and seasonality of reproduction, lower population of antral follicles at all stages of the estrous cycle, poor endocrine status and a high incidence of deep atresia in ovarian follicles, the response in terms of transferable embryo recovery has remained low with 0.51 to 3.0 per donor and pregnancy rates between 15 to 30%. In vitro production of buffalo embryos is a valid alternative to recovery of embryos by superovulation. This aspect received considerable attention during the past decade, however the proportion of embryos that develops to the blastocyst stage is still around 25-30% and hence the in vitro culture procedures need substantial improvement. Embryo cryopreservation procedures for direct transfer post thaw need to be developed for bubaline embryos. Nuclear transfer and embryo cloning is a technique that has received attention in various species during recent years and can be of immense value in buffaloes as they have a low rate of embryo recoveries by both in vitro and in vivo procedures. Gender pre-selection, genome analysis, gene mapping and gene transfer are a few of the techniques that have been studied to a limited extent during recent years and are likely to be included in future studies on buffaloes. Very recently, reproductive biotechnologies have been applied to feral buffaloes as well, but the results obtained so far are modest. When fully exploited they can play an important role in the preservation of endangered species.

• Clinical and Experimental Reproductive Medicine
• /
• v.23 no.1
• /
• pp.81-86
• /
• 1996

The assessment of acrosomal status is important in evaluating the ability of sperm to fertilize the egg. The acrosomal status of sperm from 47 normal volunteers with proven fertility and 167 subfertile men with not to achieve pregnancy for at least 1 year were evaluated with Acrobeads test(FUSO Pharmaceutical Industries, Ltd, Japan) using immunobeads coated with MH61 monoclonal antibody, which is specific for acrosome-reacted sperm. The mean${\pm}$SD of acrobeads score in 47 volunteer group was $2.8{\pm}0.7$, of which 46(97.9%)cases were ${\geq}$ 2. The mean${\pm}$SD of acrobeads score in 167 subfertile group was $1.7{\pm}0.8$, of which 73(79.3%)cases were ${\leq}$ 1. The aerobe ads score in subfertile group were significantly lower(r=0.294, p<0.05) than those in volunteer group. In subfertile group, acrobeads score were well correlated with the sperm density and motility(r=0.275, r=0.281, p<0.01), but not with semen volume(r=0.16) and serum hormone level(FSH r=0.084, LH r=0.036, testosterone r=0.058, prolactin r=0.006 and estradiol r=0.060)(p>0.05). Of 63 subfertile cases with normozoospermia, 22(34.9%)cases showed 0 or 1 of acrobeads score, which means to accompany with a functional defect in spite of normal morphology. As a results, Acrobeads test is not only a technically simple sensitive procedure with good reproducibility in evaluating the sperm fertilizing capacity but also an useful in the evaluation of effectiveness in the treatment of infertility and the separation of acrosome-reacted sperm in the assisted reproductive technique.

• Clinical and Experimental Reproductive Medicine
• /
• v.47 no.3
• /
• pp.168-179
• /
• 2020

Objective: This study investigated testicular oxidative stress status and physiomorphological function in Wistar rats fed with yaji and cadmium chloride (CdCl₂). Methods: Sixty male albino Wistar rats (12 per group) were randomly assigned to five groups: group I (control), group II (300 mg/kg.bw of yaji), group III (500 mg/kg.bw of yaji), group IV (2.5 mg/kg.bw of CdCl₂), and group V (2.5 mg/kg.bw of yaji+4 mg/kg.bw omega-3). Each group was evenly subdivided into two subgroups and treatment was administered for 14 days and 42 days, respectively. Semen quality (sperm count, progressive motility, normal morphology, and gonadosomatic index), hormones (testosterone, follicle-stimulating hormone, and luteinizing hormone), testicular oxidative stress markers (superoxide dismutase, catalase, glutathione peroxidase, and malonaldehyde) and testicular histomorphological features were examined. Results: Yaji caused significant (p< 0.05) dose- and duration-dependent reductions in semen quality, the gonadosomatic index, testosterone, follicle-stimulating hormone, and luteinizing hormone. Yaji also caused significant (p< 0.05) dose- and duration-dependent decreases in superoxide dismutase, catalase, and glutathione peroxidase activity, as well as increased testicular malonaldehyde levels. Yaji induced distortions in the testicular histological architecture. CdCl₂ damaged testicular function by significantly (p< 0.05) reducing semen quality, reproductive hormone levels, and oxidative stress markers in albino Wistar rats. CdCl₂ also altered the histology of the testis. Conclusion: This study shows that yaji sauce has similar anti-fertility effects to those of CdCl₂, as it adversely interferes with male reproduction by impairing oxidative stress markers and the function and morphological features of the testis.

• Clinical and Experimental Reproductive Medicine
• /
• v.41 no.2
• /
• pp.80-85
• /
• 2014

Objective: To investigate: the prevalence of vitamin D deficiency in Korean women with polycystic ovary syndrome (PCOS), and the relationship between vitamin D status and clinical or metabolic features in this group. Methods: We recruited 38 women with PCOS using the Rotterdam criteria. A total of 109 premenopausal control women were matched with patients based on age and body mass index. Serum 25-hydroxy vitamin D concentrations less than 20 ng/mL were classified as frank vitamin D deficiency. Since vitamin D may play a significant role in metabolic disturbances in women with PCOS, correlations between clinical or metabolic parameters and vitamin D status were analyzed separately in patients and controls. Results: Women with PCOS showed no differences in the level of 25-hydroxy vitamin D ($19.6{\pm}6.6ng/mL$ in patients vs. $20.1{\pm}7.4ng/mL$ in controls, respectively, p=0.696) or prevalence of vitamin D deficiency (57.9% in patients vs. 56.5% in controls, respectively, p=0.880). In addition, we did not find any correlations between serum vitamin D level and clinical or metabolic profiles in either PCOS patients or controls. Conclusion: Our study found no differences in the absolute level of serum vitamin D between PCOS patients and matched controls. Prevalence of vitamin D deficiency was equally common among both patients and controls. Additionally, we did not find any correlations between serum vitamin D level and clinical or metabolic profiles, suggesting that the role of vitamin D in the pathogenesis of PCOS is not yet clear.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.9
• /
• pp.5107-5110
• /
• 2013

Background: Among women with haematuria, defining individuals under high risk for bladder cancer based on reproductive factors prior to cystoscopy would be of great benefit in the management of this condition. The aim of this study was to compare age and reproductive factors such as menopausal status, parity, age at first delivery and age at the last delivery between women who have haematuria with or without bladder cancer. Materials and Methods: A total of 463 patients underwent diagnostic cystoscopy in D$\ddot{u}$zce University Faculty of Medicine between 1 June 2008 and 1 June 2013. Female patients who presented with persistent microscopic or macroscopic haematuria and underwent standard evaluation for haematuria including urinalysis, urine culture, urine cytology, urinary tract imaging with excretory urography or computerized tomography with contrast enhancement and endoscopic evaluation of the urethra and bladder were included in this study. Exclusion criteria were tobacco use and high risk occupations for bladder cancer such as textile, dry cleaning, painting and etc. Forteen women had hematuria due to benign conditions, and 18 due to bladder cancer. Data were retrospectively retrieved from the medical records of Duzce University Hospital. Results: Patients with haematuria due to benign reasons did not significantly differ from patients who were found to have bladder cancer in terms of age (p=0.28), menopausal status (p=0.29), mean parity (p=0.38), being nulliparous (p=0.57), parity ${\geq}3$ (p=0.22), age ${\leq}18$ years at first delivery (p=1.00), age ${\geq}30$ years at last delivery (p=0.26), age ${\geq}35$ years at last delivery (p=0.23) and percentage of the patients with advanced age (${\geq}65$ years) (p=0.18). Conclusions: It is difficult to predict a high risk for developing bladder cancer in women with haematuria based solely on reproductive factors.

• Korean Journal of Adult Nursing
• /
• v.22 no.6
• /
• pp.624-633
• /
• 2010

Purpose: The purpose of this study was to describe the sexual behavior, health risk related to reproductive health, and characteristics of sexual experience among college students in Korea. Methods: Six thousands college students were selected through proportional quota sampling based on region, college, and gender. Structured questionnaires were used for data collection. Descriptive statistics and chi-square test were used to describe the data. Results: Thirty eight percent (N=2,285) of the participants reported sexual intercourse. The mean age of first time sexual intercourse was 19.3 years old. Respondents reported the following health risks such as smoking, drinking, not exercising, no regular meal patterns, chronic disease status, and weight loss over 10 kg in the past three months showed significant differences by sexual intercourse experience (p<.01). Among those who had sexual intercourse experiences, 7.1% of them reported having a history of STDs and 11.6% reported a pregnancy or pregnancy of one's partner. Conclusion: The findings of this study reflect the current trends of sex behaviors among college students and correlations between health risks related to reproductive health and sexual intercourse experiences. Developing reproductive health education programs based on the findings and providing the programs to college students through a school nurse is suggested.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.5
• /
• pp.1729-1736
• /
• 2015

Background: The purpose of this study was to investigate the associations among the internal health locus of control, depression, perceived health status, self efficacy, social support, and health-promoting behavior in Iranian breast cancer survivors and to determine influential variables. Materials and Methods: A predictive design was adopted. By convenient sampling the data of 262 breast cancer survivors in Iran were collected by questionnaires during 2014. Data were analyzed applying descriptive statistics, t-tests, one-way ANOVA, Pearson's correlation coefficients, and stepwise multiple regression. Results: The internal health locus of control, depression, perceived health status, self efficacy, social support and undergoing chemotherapy all correlated significantly with the health-promoting lifestyle. Stepwise multiple regression analysis revealed that social internal health locus of control, depression, perceived health status, self efficacy and social support and chemotherapy accounted for about 39.8% of the variance in health promoting lifestyle. The strongest influence was social support, followed by self efficacy, perceived health status, chemotherapy and depression. Conclusions: The results of the study clarifed the seriousness of social support, self efficacy, perceived health status and depression in determining the health-promoting lifestyle among Iranian breast cancer survivors. Health professionals should concentrate on these variables in designing plans to promoting a healthy lifestyle.

• Fisheries and Aquatic Sciences
• /
• v.19 no.3
• /
• pp.13.1-13.8
• /
• 2016

Reproductive phenological pattern on tetrasporophytes and carposporophytes of Grateloupia subpectinata thalli were observed at Yangyang, on the middle east coast of Korea from January to December 2013. DNA sequences of cox1 gene from 48 selected samples were compared to assure that sample collection is single species, and they were all identical G. subpectinata. Density, length and width of thalli were significantly changed monthly. The maximum of density, length and fresh weight occurred in September, and continuous growth was observed during the autumn and winter. Tetrasporophytes were grown dominantly in October and November, whereas carposporophytes were dominated in September and March. Upright thalli disappeared in June and July and then juveniles were abundant in August. There was a significant difference in length between tetrasporophytes and carposporophytes. These reproductive pattern and seasonality together with phenological data of G. subpectinata would provide valuable information about successful seedling supply for aquaculture of the species in the future.