• Title/Summary/Keyword: Reproductive period

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In silico approaches to discover the functional impact of non-synonymous single nucleotide polymorphisms in selective sweep regions of the Landrace genome

  • Shin, Donghyun;Won, Kyung-Hye;Song, Ki-Duk
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.12
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    • pp.1980-1990
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    • 2018
  • Objective: The aim of this study was to discover the functional impact of non-synonymous single nucleotide polymorphisms (nsSNPs) that were found in selective sweep regions of the Landrace genome Methods: Whole-genome re-sequencing data were obtained from 40 pigs, including 14 Landrace, 16 Yorkshire, and 10 wild boars, which were generated with the Illumina HiSeq 2000 platform. The nsSNPs in the selective sweep regions of the Landrace genome were identified, and the impacts of these variations on protein function were predicted to reveal their potential association with traits of the Landrace breed, such as reproductive capacity. Results: Total of 53,998 nsSNPs in the mapped regions of pigs were identified, and among them, 345 nsSNPs were found in the selective sweep regions of the Landrace genome which were reported previously. The genes featuring these nsSNPs fell into various functional categories, such as reproductive capacity or growth and development during the perinatal period. The impacts of amino acid sequence changes by nsSNPs on protein function were predicted using two in silico SNP prediction algorithms, i.e., sorting intolerant from tolerant and polymorphism phenotyping v2, to reveal their potential roles in biological processes that might be associated with the reproductive capacity of the Landrace breed. Conclusion: The findings elucidated the domestication history of the Landrace breed and illustrated how Landrace domestication led to patterns of genetic variation related to superior reproductive capacity. Our novel findings will help understand the process of Landrace domestication at the genome level and provide SNPs that are informative for breeding.

Biochemical parameters and reproductive traits in female rabbits (oryctolagus cuniculus) exposed to psidium guajava leaf aqueous extract

  • Azafack Kana Dorice; Paguem Eric Achile;Deutcheu Nienga Sorelle;Tchoffo Herve;Chongsi Margaret Momo;Ngwafu Nancy Ngwasiri;Ferdinand Ngoula
    • Journal of Animal Reproduction and Biotechnology
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    • v.38 no.3
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    • pp.151-157
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    • 2023
  • Background: The potential impact of aqueous extracts from Psidium guajava leaves on the reproductive system of female rabbits was evaluated. Methods: Twenty-eight rabbits, aged five to six months were utilized. Rabbits were divided into four groups and were randomly assigned to receive one of the following oral doses of the guava leaf extracts: 0 (control group), 10, 20, or 30 mg/kg of body weight. After a treatment period of 30 days, blood was collected via jugular venipunture and the serum was extracted for the assessment of serum biochemical traits levels. The females were bred and monitored throughout their pregnancy to ascertain reproductive outcomes. Results: The results indicated that the guava leaf extract significantly increased the body weight of the rabbits during both pre- and post-pregnancy compared to the control group (p < 0.05). The litter size at three weeks post-birth, prolificity rate, FSH, LH, and protein levels were notably higher (p < 0.05) at a dose of 20 mg/kg of body weight. The viability rate three weeks post-birth increased with escalating extract doses, and the highest values were observed at doses of 20 and 30 mg/kg of body weight (p < 0.05). Conclusions: This study demonstrated that, the aqueous extract of guava leaves appears to stimulate the production of FSH, LH and enhance body weight, prolificity, and pregnancy outcomes in mammals. As such, it is suggested that a dose of 20 mg/kg body weight could be beneficial in improving the reproductive performance of female.

The Technical and Financial Effects of Parenteral Supplementation with Selenium and Vitamin E during Late Pregnancy and the Early Lactation Period on the Productivity of Dairy Cattle

  • Bayril, T.;Yildiz, A.S.;Akdemir, F.;Yalcin, C.;Kose, M.;Yilmaz, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.8
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    • pp.1133-1139
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    • 2015
  • This study aimed to determine the effects of parenteral selenium (Se) and vitamin E supplementation on economic impact, milk yield, and some reproductive parameters in high-yield dairy cows in the dry period and in those at the beginning of lactation. At the beginning of the dry period, cows (n = 323) were randomly divided into three groups as follows: Treatment 1 (T1), Treatment 2 (T2), and Control (C). Cows in group T1 received this preparation 21 days before calving and on calving day, and cows in group T2 received it only on calving day. The cows in the control group did not receive this preparation. Supplementation with Se increased Se serum levels of cows treated at calving day (p<0.05). Differences in milk yield at all weeks and the electrical conductivity values at the 8th and 12th weeks were significant (p<0.05). Supplementation with Se and Vitamin E decreased the incidence of metritis, the number of services per conception and the service period, but had no effects on the incidence of retained fetal membrane. A partial budgeting analysis indicated that Se supplementation was economically profitable; cows in group T1 averaged 240.6$ per cow, those in group T2 averaged 224.6$ per cow. Supplementation with Se and Vitamin E has been found to increase serum Se levels, milk yield, and has positive effects on udder health by decreasing milk conductivity values and incidence of sub-clinical mastitis.

Influence of Drought Period in Different Growth Stage on Agronomic Characters in Sesame (참깨 생육기별 한발기간이 주요형질에 미치는 영향)

  • 최형국;김용재;구자옥;최원열;김학진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.4
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    • pp.295-303
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    • 1990
  • In this study, drought period when important agronomic characters of sesame plant is critical, was examined at different growth stages. Plant death by drought started at 40 days after drought in vegetative growth stage and 20 days, in reproductive growth stage. Obserbed by growth stage, drought damage in reproductive growth stage was more in jurious than vegetative growth stage. All the important agronomic characters was refreshed until 40 days and 20 days after drought in vegetative growth stage and reproductive growth stage respectively, but it could berefreshed after those times. Decrease rate of yield by drought ranged from 29 to 80% in vegetative growth stage and from 49 to 85% in respective growth stage. All the important agronomic characters except rate of ripeness showed positive association with grain yield under drought condition. Oil content in grain was decreaced by drought but composition of fatty acid was not affected by it.

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Possible Involvement of Photoperiodic Regulation in Reproductive Endocrine System of Female Olive Flounder Paralichthys olivaceus

  • Kim, Hyun Chul;Lee, Chi Hoon;Hur, Sung Pyu;Kim, Byeong Hoon;Park, Jun Young;Lee, Young Don
    • Development and Reproduction
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    • v.19 no.1
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    • pp.11-17
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    • 2015
  • This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-${\beta}$, LH-${\beta}$ and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

Reproductive Biology of the Purplish Washington Clam, Saxidomus prupuratus in the East China Sea (동중국해에 분포하는 개조개의 생식생물학적 연구)

  • Kim, Young-Seop;Kim, Yeong-Hye;Kim, Jon-Bin;Chang, Dae-Soo;Lee, Dong-Woo;Kang, Hyun-Jung
    • The Korean Journal of Malacology
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    • v.26 no.4
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    • pp.261-265
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    • 2010
  • Reproductive biology of the purplish Washington clam, Saxidomus purpuratus was investigated based on the samples captured in Jinhae Bay, the East China Sea from January to December 2002. The gonad index (GI) began to increase in January, reached the maximum value in March. The reproductive cycle of this species can be divided into five successive stages: the early active stage (from November to January), the late active stage (from December to February), the ripe stage (from February to May and October), the spawned stage (from May to December), and inactive stage (from November to December). The spawning period was from April to December, and the main spawning occurred between June and August. The shell length at 50% group maturity was estimated to be 71.85 mm. The Sex ratio of this species was not significantly different a 1:1 sex ratio (P > 0.05).

Detection of antibody to porcine reproductive and respiratory syndrome virus from pig sera collected during the period of January to December 2000

  • Jung, Hae-Sun;Kim, Su-Mi;Kim, Jong-Taik;Han, Tae-Uk;Kang, Shien-Young;Shin, Kwang-Soon;Kim, Chul-Joong;Park, Bae-Keun;Kim, Hyun-Soo
    • Korean Journal of Veterinary Service
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    • v.24 no.4
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    • pp.343-346
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    • 2001
  • During the period of January to December 2000, a total of 3,505 swine sera was collected from 208 farms, which are located throughout country, for the diagnosis of porcine reproductive and respiratory syndrome(PRRS). The antibody to porcine reproductive and respiratory syndrome virus(PRRS) was tested by indirect immunofluorescent antibody(IFA) test. Of 208 farms tested, at least one or more than one pigs was positive for PRRSV antibody in 188(90.4%) farms. The overall seroprevalence of PRRSV antibody was 45.1% (1581/3505). Most pigs were infected with PRRSV at around 50- to 60-day old. The seroprevalence of antibody varied with age. The highest seroprevalence of PRRSV antibody was observed in the growing pigs at around 80-day old. About one-thirds of adult pigs including boar, gilt and sow were positive to PRRSV antibody. In many farms, the infection of PRRSV was chronic and confined to grower and/or finisher. However, antibody was detected from all production phase in some farms.

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Glioma Epidemiology in the Central Tunisian Population: 1993-2012

  • Trabelsi, Saoussen;Brahim, Dorra H'mida-Ben;Ladib, Mohamed;Mama, Nadia;Harrabi, Imed;Tlili, Kalthoum;Yacoubi, Mohamed Tahar;Krifa, Hedi;Hmissa, Sihem;Saad, Ali;Mokni, Moncef
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.20
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    • pp.8753-8757
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    • 2014
  • Background: Glioma is a heterogeneous central nervous system (CNS) tumor group that encompasses different histological subtypes with high variability in prognosis. The lesions account for almost 80% of primary malignant brain tumors. The aim of this study is to extend our understanding of the glioma epidemiology in the central Tunisian region. Materials and Methods: We analyzed 393 gliomas recorded in cancer registry of central Tunisia from 1993 to 2012. Crude incidence rates (CR) and world age-standardized rates (ASR) were estimated using annual population data size and age structure. Statistic correlations were established using Chi-square and Kaplan-Meier test. Results: Tunisian glioma patients were identified with a mean age at diagnosis of 48 years and 1.5 sex ratio (male/female). During the 19 years period of study the highest incidence value was observed in male group between 1998 and 2002 (CR: 0.28, ASR: 0.3). Incidence results underline increasing high grade glioma occurring in the adulthood in the last period (2007-2012). Median survival was 27 months, with 1-, 2- and 5-year survival rates of 42%, 30% and 26%, respectively. Survival was greater in patients with younger age, lower tumor grade, infratentrial tumor location and undergoing a palliative treatment. Conclusions: This central Tunisia gliomas registry study provides important information that could improve glioma management and healthcare practice.

Improving behavior characteristics and stress indices of gestating sows housed with group housing facility

  • Jeong, Yongdae;Choi, Yohan;Kim, Doowan;Kim, Joeun;Min, Yejin;Jung, Hyunjung;Kim, Younghwa
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.875-883
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    • 2020
  • This study was conducted to investigate the effects of group-housing facility (GHF), compared to an individual confinement stall (CON), on the reproductive performance, behavior, and stress hormones of gestating sows. A total of 50 primiparous sows (Landrace × Yorkshire) were randomly allocated into either CON (n = 25) or GHF (n = 25) during the gestation period. One week before parturition, the sows were transferred into conventional farrowing crates, and cross-fostering was conducted within 1 d of delivery. Blood was collected for analyses of stress indices at 75 d of gestation and postpartum. Reproductive performance was estimated during the period of birth to weaning. Behavior patterns were identified at 90 d of gestation. Litter size was not different between the CON and GHF treatments. Weaning to estrus interval, however, tended to be lower in the GHF than in the CON (p < 0.1). Activity, treating, belly nosing, and exploring behaviors were observed only in the GHF group, whereas rubbing was shown only with the CON. Serum cortisol concentration was lower in the GHF than in the CON at 75 d of gestation (p < 0.05). Sows housed in the GHF showed lower epinephrine and norepinephrine concentrations than those housed in the CON at postpartum (p < 0.05). The GHF sows demonstrated more natural behavior characteristics associated with stress relief than the CON sows with no adverse effects on reproductive performance. Therefore, these results suggest that GHF could be applied as an alternative housing facility to improve animal welfare on swine farms.

Effect of xylo-oligosaccharides on reproduction, lipid metabolism, and adipokines of hens during the late egg-laying period

  • Wen, Fengyun;Wang, Fengyan;Li, Pei;Shi, Hanyi;Liu, Ning
    • Animal Bioscience
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    • v.35 no.11
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    • pp.1744-1751
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    • 2022
  • Objective: The present study aimed to investigate the effect of xylo-oligosaccharides (XOS) administration on egg production, reproductive hormones, serum lipids and adipokines of hens at the late cycle of reproduction. Methods: Four treatments included control (basal diet) and XOS addition at 2.0 (XOS-2), 4.0 (XOS-4), or 6.0 (XOS-6) g/kg of diet using 288 commercial Hy-Line brown hens from 73 to 84 wk of age. Egg production, body fat deposition, reproductive tract and hormones, lipid metabolism and adipokines were determined. Results: At 84 wk, compared to the control, XOS supplementation at the three doses increased (p<0.001) egg-laying rates by 13.2% averagely, which led to a higher egg mass by 131 g/hen throughout the whole trial period. Abdominal fat and skinfold of XOS treatments were decreased (p<0.001) by 26.1% and 18.6%, respectively; large follicles and ovary weight were increased (p<0.001) by 0.73 follicle/hen and 18.6%, respectively. For serum parameters, cholesterol and triglyceride were decreased (p<0.001) by 17.5% and 29.2%, respectively; luteinizing hormone, follicle-stimulating hormone, and progesterone were increased (p≤0.001) by 16%, 31%, 29%, respectively; adiponectin and visfatin were increased (p<0.001) by 34% and 44%, respectively; but chemerin and leptin were decreased (p≤0.001) by 22% and 14%, respectively. With the increased XOS doses, linear decreases (p<0.05) were found on abdominal skinfold and serum triglyceride. Conclusion: The obtained data indicate that XOS can be used as an additive to improve fecundity by beneficially modulating fat deposition, lipid metabolism, reproductive hormones, and adipokines of hens at the late cycle of reproduction.