Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Biochemical parameters and reproductive traits in female rabbits (oryctolagus cuniculus) exposed to psidium guajava leaf aqueous extract

  • Azafack Kana Dorice (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Paguem Eric Achile (Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, University of Buea) ;
  • Deutcheu Nienga Sorelle (Department of Animal Sciences, Faculty of Agriculture and Veterinary Medicine, University of Buea) ;
  • Tchoffo Herve (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Chongsi Margaret Momo (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Ngwafu Nancy Ngwasiri (Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, University of Buea) ;
  • Ferdinand Ngoula (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang)
  • Received : 2023.08.09
  • Accepted : 2023.08.27
  • Published : 2023.09.30
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Abstract

Background: The potential impact of aqueous extracts from Psidium guajava leaves on the reproductive system of female rabbits was evaluated. Methods: Twenty-eight rabbits, aged five to six months were utilized. Rabbits were divided into four groups and were randomly assigned to receive one of the following oral doses of the guava leaf extracts: 0 (control group), 10, 20, or 30 mg/kg of body weight. After a treatment period of 30 days, blood was collected via jugular venipunture and the serum was extracted for the assessment of serum biochemical traits levels. The females were bred and monitored throughout their pregnancy to ascertain reproductive outcomes. Results: The results indicated that the guava leaf extract significantly increased the body weight of the rabbits during both pre- and post-pregnancy compared to the control group (p < 0.05). The litter size at three weeks post-birth, prolificity rate, FSH, LH, and protein levels were notably higher (p < 0.05) at a dose of 20 mg/kg of body weight. The viability rate three weeks post-birth increased with escalating extract doses, and the highest values were observed at doses of 20 and 30 mg/kg of body weight (p < 0.05). Conclusions: This study demonstrated that, the aqueous extract of guava leaves appears to stimulate the production of FSH, LH and enhance body weight, prolificity, and pregnancy outcomes in mammals. As such, it is suggested that a dose of 20 mg/kg body weight could be beneficial in improving the reproductive performance of female.

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References

  1. Adaay MH and Mosa AAR. 2012. Evaluation of the effect of aqueous extract of Tribulus terrestris on some reproductive parameters in female mice. J. Mater. Environ. Sci. 3:1153-1162. 
  2. Adebayo JO, Adesokan AA, Olatunji LA, Buoro DO, Soladoye AO. 2005. Effect of ethanolic extract of Bougainvillea spectabilis check for this species in other resources leaves on haematological and serum lipid variables in rats. Biokemistri 17:45-50. 
  3. Couchman GM and Hammond CB. 1999. Physiology of reproduction. In: Sott JR, Di Saia PJ, Hammond CB, Spellacy WN (Eds.), Danforth's Obstetrics and Gynecology, Lippincott, Williams & Wilkins, Philadelphia, pp. 47-64. 
  4. Ekaluo UB, Erem FA, Omeje IS, Ikpeme EV, Ibiang YB, Ekanem BE. 2013b. Is aqueous leaf extract of guava spermatotoxic in rat? IOSR J. Environ. Sci. Toxicol. Food Technol. 3:21-23.  https://doi.org/10.9790/2402-0322123
  5. Ferdinand N, Christiane NS, Augustave K, D'Alex TC, Raphael KJ, Jean-Marc K, Borice T, Herman NV, Maryvone NT, Herman A, Djalil OIA, Pierre K, Galeotti M, Joseph T. 2014. Effect of guava (Psidium guajava) leaves essential oil on some reproductive parameters in male guinea pig (Cavia porcellus). Biol. Syst. Open Access 3:125. 
  6. Gayrard V. 2007. Physiologie de la Reproduction des Mammiferes. Ecole Nationale Veterinaire, Toulouse, p. 198. 
  7. Gornall AG, Bardawill CJ, David MM. 1949. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 177:751-766.  https://doi.org/10.1016/S0021-9258(18)57021-6
  8. Grignard E. 2005. Analyse de proteines spermatiques posttesticulaires et developpement d'outils pour le controle de la fertilite de differents mammiferes; Equus caballus, Bos taurus, Arvicola terrestris Scherman [Doctoral dissertation, Universite Blaise Pascal]. HAL. https://theses.hal.science/tel-00677284 
  9. Hazard J, Perlemuter L, Abramovici Y, Bourgeon M. 2000. Endocrinologie. 4th ed, Masson, Paris, p. 484. 
  10. Hernandez F, Madrid J, Garcia V, Orengo J, Megias MD. 2004. Influence of two plant extracts on broilers performance, digestibility, and digestive organ size. Poult. Sci. 83:169-174.  https://doi.org/10.1093/ps/83.2.169
  11. Ibe AE and Nwufo MI. 2005. Identification, collection and domestication of medicinal plants in Southeastern Nigeria. Afr. Dev. 30:66-77. 
  12. Konstantopoulou I, Vassilopoulou L, Mavragani-Tsipidou P, Scouras ZG. 1992. Insecticidal effects of essential oils. A study of the effects of essential oils extracted from eleven Greek aromatic plants on Drosophila auraria. Experientia 48:616-619.  https://doi.org/10.1007/BF01920251
  13. Kullu AR, Tabassum W, Sinha MP. 2013. Effects of Psidium guajava aqueous extracts on haematological profile and serum lipid variables of albino rats. Bioscan 8:743-746. 
  14. Lieberman S. 1996. Are the differences between estradiol and other estrogens, naturally occurring or synthetic, merely semantical? J. Clin. Endocrinol. Metab. 81:850-851.  https://doi.org/10.1210/jcem.81.2.8636315
  15. Mathlouthi N, Bouzaienne T, Oueslati I, Recoquillay F, Hamdi M, Bergaoui R. 2009. [The effect of two essential-oil preparations on in vitro of bacterial growth and performances of broiler chickens]: Proceedings of the 8th Avian French Research Days. Beekbergen: World Poultry Science Association. 
  16. Mommsen TP and Walsh PJ. 1991. Urea synthesis in fishes: evolutionary and biochemical perspectives. In: Hochachka PW and Mommsen TP (Eds.), Phylogenetic and Biochemical Perspectives, Biochemistry and Molecular Biology of Fishes, Elsevier, Amsterdam, pp. 137-163. 
  17. Natumanya R, Owiny OD, Kugonza DR. 2008. The potential of Ankole cattle abattoir ovaries for in vitro embryo production. Afr. J. Anim. Biomed. Sci. 3:1819-4214. 
  18. Recoquillay F. 2009. L'interet des huiles essentielles: Proceedings of 9eme Journee Productions porcines et avicoles. pp. 55-61. 
  19. Riviere R. 1991. Manuel d'Alimentation des Ruminants Domestiques en Milieu Tropical. 2nd ed, Ministere de la Coopération et du Developpement, Paris, p. 529. 
  20. Ryu NH, Park KR, Kim SM, Yun HM, Nam D, Lee SG, Jang HJ, Ahn KS, Kim SH, Shim BS, Choi SH, Mosaddik A, Cho SK, Ahn KS. 2012. A hexane fraction of guava Leaves (Psidium guajava L.) induces anticancer activity by suppressing AKT/mammalian target of rapamycin/ribosomal p70 S6 kinase in human prostate cancer cells. J. Med. Food 15:231-241.  https://doi.org/10.1089/jmf.2011.1701
  21. Slaughter MS, Rogers JG, Milano CA, Russell SD, Conte JV, Feldman D, Sun B, Tatooles AJ, Delgado RM 3rd, Long JW, Wozniak TC, Ghumman W, Farrar DJ, Frazier OH. 2009. Advanced heart failure treated with continuous-flow left ventricular assist device. N. Engl. J. Med. 361:2241-2251. (Erratum published 2018, N. Engl. J. Med. 379:697) 
  22. Uboh FE, Edet EE, Eteng MU, Eyong EU. 2010a. Comparative effect of aqueous extract of P. guajava leaves and ascorbic acid on serum sex hormones levels in male and female rats. J. Appl. Sci. Res. 6:275-279. 
  23. Ugwah-Oguejiofor CJ, Bello SO, Okolo RU, Etuk EU, Ugwah MO, Igbokwe VU. 2011. Ficus platyphylla promotes fertility in female Rattus norvegicus Wistar strain: a preliminary study. Reprod. Biol. Endocrinol. 9:145. 
  24. Yakubu MT, Akanji MA, Oladiji AT. 2005. Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats. Asian J. Androl. 7:399-404. https://doi.org/10.1111/j.1745-7262.2005.00052.x