• Applied Microscopy
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• v.12 no.2
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• pp.11-22
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• 1982

The morphological development of Mycoplasma pneumoniae attached to solid surfaces was examined by light and electron microscopies. Critical point drying and carbon replication techniques revealed that during the growth cycle of developing microcolonies, the morphological form coincided with the pH of the culture. M. pneumoniae appeared to have a well defined morphology associated with age of the culture. The organisms were dimorphic, with round cells capable of reproduction and segments consisting of a spindle shaped body with one pointed and one knob-like end. Starting with single cells, there were the following stages in the development of a culture: replication stage through binary fission and segmentation, stage of confluency, and a degeneration stage into rough spherical forms. The round cells appearrd to replicate by binary fission during the lag and early log phases of growth, while spindle segments replicated by segmentation during most of the logarithmic growth. The growth of the filaments and replication of the segments occured at the knob-like ends, showing a type of polarity, and formed a meshwork across the surface. This development could be cycled under favorable growth conditions, but the culture aged and when the conditions became adverse(e.g. pH 6.8 or lower), filamentous cells converted to spherical forms, losing their reproductive capability.

• Journal of Embryo Transfer
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• v.27 no.1
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• pp.15-20
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• 2012

The objective of this study was to investigate the relationship between body weight, body condition score (BCS), blood urea nitrogen (BUN), glucose, cholesterol and number of transferable embryos for the purpose of improving reproductive performance in Hanwoo donors. Seventy five cows, at random stages of the estrous cycle, received a CIDR together with injection of 1mg estradiol benzoate and 50 mg progesterone, and gonadotropin treatment begann. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU = 1 ml) administered twice daily in constant doses over 4 days. On the 3rd administration of FSH, CIDR was withdrawn and 25 mg $PGF_2{\alpha}$ was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received $100{\mu}g$ GnRH at the time of 1st insemination. Embryos were recovered 7 days after the 1st insemination. In conclusion, cows with body weight < 400, 400~450 and > 450kg had number of transferable embryos of $4.2{\pm}1.7$, $6.1{\pm}2.7$ and $4.8{\pm}2.6$, cows with BCS <2.25, 2.25~2.75 and ${\geq}2.75$ had number of transferable embryos of $4.6{\pm}1.6$, $5.7{\pm}2.4$ and $5.1{\pm}2.7$ respectively. These data indicate that a body weight and BCS for superovulation of CIDR-treated Korean native cows does not affect the embryo yield.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.5
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• pp.327-339
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• 1991

The present paper is a histological study of neurosecretory cells in the brain and the thoracic ganglion with the gonadal development in Palaemon serrifer. The reproductive cycle includes the successive stages of the growing period (February-March), the mature period(April-May), the ripe and spent periods(June-August) and the degenerative and resting periods(September-January). The neurosecretory cells are grouped into four types based on Matsumoto(1958) : A-,A'-, B- and E-cells. A- and A'-cells are $80-90{\mu}m,\;B-cell\;is\;30-40{\mu}m$ and E-cell is $10-15{\mu}m$. A- and B-cells are the positive to CHP and AF, while B-cell is the positive only to AF. The secretory grannules of a A-cell are transported to the axon, and at the same time they are discharged through the peripheral membrane. Of the four neurosecretory cells, A- and I-cells show the difference of secretory activity according to the gonad developmental process. In the female, A-cells show secretory activity for the ripe and spent periods, while I-cells show for the mature, ripe and spent periods. In the male, A-cells show secretory activity for the mature, ripe and spent periods, while I-cells show for the growing, mature, ripe and spent periods.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.4
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• pp.302-306
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• 2000

Gonad and blood samples were taken from cultured greenling (Hexagrammos otakii), and changes of steroid hormone levels in serum were examined in relation to the gonadosomatic index (GSI). GSI of female and male were not changed significantly between March and August and then began to increase from September showing the highest value in January. Hepatosomatic index (HSI) of female was the highest in December,'then decreased gradually until May. $Estradiol-l7{\beta} (E_2)$ level in blood of female reached to the highest level in December, then decreased rapidly in January. The level was maintained low until August and started to increase In September. Testosterone (T) level in blood of female was similar to trend of $E_2$ in female. $17{\alpha}2{\beta}-dihydroxy-4-pregnen-3-one (17{\alpha}20{\brta}-OHP)$ level in blood of female reached to the maximum in January, and then decreased rapidly from Febuary and maintained low until July. T level in blood of male reached to the highest value in December, and it started to decrease from January and maintained low until July, after then increased again, while $17{\alpha}20{\beta}-OHP$ level of male was similar to trend of female's.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.5
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• pp.713-720
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• 1998

Experiments on the control of rockfish, Sebastes inermis reproduction were performed by artificially changed water temperature (WT) and photoperiod (PP). The experiments were divided into three conditions; artificial changes of both WT and PP delayed two months against their natural variations (Exp.I), natural WT and PP replaced with artificial light (Exp.II) and natural WT and PP (Exp.III) from September to April (220 days). Initial gonadosomatic index (GSI) in female groups of experiments was 0.2. Female GSI of responded group in Exp.I began to increase from December and reached the highest value in February with 15.0, while the un-responded group kept the lower value during the experimental period. Female GSI of Exp.II and Exp.III reached the highest values in January with 13.0 and 14.7, respectively. Male GSI of Exp,I reached the maximum in October, and that of Exp.II and III reached the maximum in November and then rapidly decreased. Among the experimental groups, male GSI did not show the different tendency. Monthly changes of hepatosomatic index in both sexes showed the reciprocal tendency with those of GSI. The control of WT and PP did not affect on the changes of HSI and condition factor of rockfish. From the histological observations, maturation of ovary was delayed by the changes of WT and PP, but that of testis was not influenced. Judging from the above results, the maturation and parturition of female rockfish could be delayed one or two months by artificial changes of WT and PP.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.4
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• pp.234-241
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• 1975

Reproductive cycle of Haliotis discus hannai Ino was studied based on the samples which were collected in Yeosu area from January, 1973 to December, 1974. Particular emphasis was paid on the seasonal variation of gonad maturity factor, coefficieot of gonad maturity, fatnesss, and relationship of gonad weight to growth of the abalone. seasonal fluctuation of water temperature and induction of artificial spawnings were also checked. The abalone of this area spawns twice a year: spring spawning(early May-late July) and Autumn spawning(early September-late November). peak spawning occurs from late May to middle of June for spring spawning: it occurs from late September to early November for Autumn spawning. Gonad maturity factor was closely related to the spawning seasons. For the spring spawning Gonad maturity factor reached to its maximum value as 18.84 in April; for the Autumn spawning it reached to its maximum value as 22.65 in September. Annual minimum value was 12.65 in January. Annual maximum values of coefficient of gonad maturity were 44.10 in May and 49.05 in September and the annual minimum was 10.09 in January. Fatnesswas highest in July as 0.221 and it was lowest in October as 0.1058. Ratios of shell length to gonad weight were formulated as follows : $$In\;September\;GW=0.000003710SL^{3.7860}$$ $$In\;January\;GW=0.000021198SL^{2.8564}$$ $$Annual\;mean\;GW=0.00000005013SL^{4.2273}$$ Relations of total weight to gonad weight were formulated as follows : In September GW=0.1918TW-4.9231 In January GW=0.0992TW-0.1759 Annual maan GW=0.1568UW-3.5256.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.3
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• pp.167-176
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• 1991

Sexual maturation of the bluespotted mud hopper, Boleophthalmus Pectinirostris(Linnaeus) was investigated histologically on the gonadal development, and studied by gonadosomatic index(GSI), egg diameter composition. Samples were collected in the intertidal zone of Wolyon-ri, Hoihyon-myon, Okku-gun, Chollabuk-do, Korea, from April to October in 1988 and from June to August in 1989. The ovary is a pair of sac-shaped organ. The testis is a pair of tubule-shaped organ and it is connected to the seminal vesicle which is located at the posterior end of the testis. In male and female, GSI began to increase from late May when the water temperature began to increase and reached the maximum value in June and July, respectively. It began to decrease from August, the highest water temperature season. Thereafter, maintained relatively low values until October. The annual reproductive cycle of this species could be classified into four sucessive developmental stages: growing stage$(April{\~}May)$, mature stage$(June{\~}early\;July)$, ripe and spent stage(late lune-early August), degenerative and resting stage$(late\;August{\~}March:\;the wintering\;period)$. According to the frequency distributions of egg diameters in the spawning season, Boleophthalmus Pectinirostris was species to spawn twice or more in the spawning season.

• Korean Journal of Ichthyology
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• v.6 no.2
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• pp.222-236
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• 1994

Sexual maturation and spawning of the spottybelly greenling, Hexagrammos agrammus were histologically studied under photomicroscopy by considering changes in gonadosomatic index, hepatosomatic index and fatness, egg diameter composition, first sexual maturity and fecundity. The fish samples were collected monthly at the coastal area of Tongbaeksom, Pusan, Korea from July 1991 to July 1992. The gonadosomatic index(GSI) increased in September and reached the maximum value(female 4.31, male 1.61) in November when the gonad was mature and ripe. The values were decreased suddenly during the spawning season from December, and declined from January to August. The annual variations of hepatosomatic index(HSI) appeared to be correlated with those of GSI in female but were not significantly correlated in male. HSI in female began to increase in autumn with the increase of GSI, and reached the maximum in winter when the ovary was mature. Percentages of first sexual maturity in female and male fish were 50 % in 11.0~11.9 cm and 100 % in 13.0~13.9 cm groups. Both sexes participated in reproduction from one year old. H. agrammus was considered as a polycyclic species and spawns 3 times or more in the spawning season. Number of total and mature eggs in the absolute fecundity were proportional to standard length and body weight, respectively. Number of total and mature eggs in relative fecundity were also proportional to standard length, but rather decrease with increasing of body weight. Fatness coefficients reached the maximum value(female 15.32, male 15.14) in September(growing stage), and the values were sharply decreased after spawning. Thereafter, fatness values were gradually increased, therefore, the monthly changes in fatness coefficient closely correlated with the reproductive cycle. Sex ratios of female and male sexes of this species are showed 54.18 %, 45.82 %, respectively.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.73-79
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• 2012

Seasonal changes in biochemical composition of muscle, gonad-viceral, mass and whole body of the cultured ark shell, Scapharca broughtonii in the Gamag bay of Yeosu city were studied from December 2008 to November 2009 in relation to environmental condition and reproductive cycles. Average monthly water temperature in the winter was in the range of $7-12^{\circ}C$ and $20-25^{\circ}C$ in the summer, while the salinity fluctuated in the range of 30.1%-33.8‰ on the average. Seasonal fluctuation of the concentration of nutrient salt was the highest in September ($13.04{\mu}g/L$) with average annual concentration of $4.6{\mu}g/L$. The main spawning season of the ark shell was during the months of July and August, and the gonads were in inactive stage during the winter. The gonad-visceral mass contained lower amounts of proteins than the other body parts. The most marked changes in body composition were lipids and carbohydrates within the gonad-visceral mass, and protein for each of the organs was relatively consistent throughout the year. All the parts in the visceral sac displayed the highest changes during the gametogenic cycle while the contents of moisture and lipid within the visceral act displayed somewhat inverse relations with each other. Moisture content was the lowest during the inactive stage during which the lipid content is the highest. The lipid content was the lowest immediately following spawning with increase in the moisture content as the lipid is being consumed. Protein mass within the visceral sac was low in comparison to the muscle mass. It is deemed that carbohydrates, lipids and proteins in the visceral sac play the major role as the source of energy during the development process of the gonads, and used for maintenance of base metabolism when available food is scarce.

• Korean Journal of Psychosomatic Medicine
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• v.3 no.2
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• pp.115-125
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• 1995

Reports about the prevalence of premenstrual symptoms state that occurs in 20 to 100% of most reproductive-age women. There is a close association between premenstrual syndrome and affective disorders as well as same some other psychiatric disorders. Late luteal phase dysphoric disorder (LLPDD) is a premenstrual condition defined in DSM-III-R by severe mood changes and other symptoms that repeatedly occur only in the luteal phase of the menstrual cycle. However, DSM-III-R does not specify how to compute the change from the follicular to the luteal phase or how to determine when the amount of change is great enough to warrant the diagnosis nor how to determine occupational or social functional impairment. This study was conducted to evaluate the nature, severity and magnitude of premenstrual syndrome in women with current psychiatric disorders by using prospective Daily Rating Form(DRF), and to measure symptom changes according to three scoring methods for diagnosing LLPDD. Our study obtains the data about premenstrual changes estimated by DRF from 22 women with psychiatric disorders who had met criteria for major depressive syndrome on the Premenstrual Assessment Form (PAF). The data was scored by each three methods and was determined to meet criteria A for LLPDD. The results are as follows: 1) the subjects, when scored according to the percent change method, effect size method and absolute severity method, met the DSM-III-R criteria A for LLPDD in 36.4% (8 subjects), 14% (3 subjects) and 4.5% (1 subject) of the cases respectively. 2) The items of irritability, anger and impatience were occurred most frequently on the DRF, when it was scored according to the three scoring methods. And the item of breast pain was next frequent according to the effect size method and the percent change method but according to the absolute severity method. 3) The PAF item of impaired social functioning was reported by 16 (73%) of the subjects. 4) 4 (18%) of the subjects met criteria A for LLPDD and reported impaired social functioning. The prevalency of LLPDD according to each method varied. The percent change method yielded the greatest (36.4%), and the absolute seventy method yielded the laest (4.5%), The effect size method yielded an intermediate frequency (14%). Therefore, for maximizing the diagnostic accuracy of LLPDD, a diagnostic procedure including a measure of change (e.q., effect size method, percent change method) as well as confirmed diagnosis by DRF, will be needed. Also, an accurate tool to evaluate impaired social functioning will be required.