• 제목/요약/키워드: Reproduction study

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Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells

  • Li, Bo-jiang;Li, Ping-hua;Huang, Rui-hua;Sun, Wen-xing;Wang, Han;Li, Qi-fa;Chen, Jie;Wu, Wang-jun;Liu, Hong-lin
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.8
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    • pp.1171-1177
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    • 2015
  • The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.

Knowledge, Media and Information in the Age of Digital Reproduction (디지털 복제 시대의 지식, 미디어, 정보 - 지식의 기술.사회적 조건 변화를 중심으로)

  • Paik, Wook-Inn
    • Korean journal of communication and information
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    • v.49
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    • pp.5-19
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    • 2010
  • The purpose of this paper is to disclose the characteristics of knowledge in the age of digital reproduction. To achieve this I extended Benjamin's concept of technological reproduction to three different stages as mechanical reproduction, electric reproduction and digital reproduction. Each stage has different degree of reproducibility and de-materialization. I also combined McLuhan's concept of the electricity at the point of de-materialization with reproducibility. The degree of materiality and reproducibility is the key concept which could scale the technical condition of knowledge production. The social condition of knowledge reproduction is controlled by economic and political factors. In this connection between technological and social condition the current knowledge production and intellectual property could be explained properly. This study could open the theoretical door to deepen such an understanding.

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Difference of Proprioceptive Sense at Elbow Joint According to Measurement Methods (팔굽관절에서 측정방법에 따른 고유수용성감각 차이)

  • Lee, Jung-Ah;Kim, Duk-Hwa;Shin, Hwa-Kyung;Choi, Kyu-Hwan;Kwon, Oh-Yun
    • Physical Therapy Korea
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    • v.10 no.3
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    • pp.63-70
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    • 2003
  • The purpose of this study was to compare the difference of joint position sense between measurements. Fourteen healthy male subjects were recruited for this study. The elbow joint position senses were measured using angle reproduction test. The elbow joint position sense was assessed with three experimental conditions: ipsilateral reproduction test in open-chain condition, contralateral reproduction test in open-chain condition, ipsilateral reproduction test with weight in open-chain condition and ipsilateral reproduction test in closed-chain condition. The angular difference between stimulus position and the reproduced position (angular error) was calculated in all testing conditions to examine the accuracy of the joint position sense. One way ANOVA was used to compare the error angles in all experimental conditions. The error angles between measurements were significantly different in elbow joint. The error angles was smallest in ipsilateral reproduction test with weight in open-chain condition and was greatest in the contralateral reproduction test in open-chain condition. Findings of this study indicate that testing methods, types of task, existence of resistance should be considered in clinical assessment for the joint position sense.

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A Comparative Analysis of the Library Exceptions in Copyright Laws in Major Countries (주요국 저작권법의 도서관 예외 규정 비교 분석)

  • Joung, Kyounghee
    • Journal of the Korean Society for information Management
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    • v.34 no.1
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    • pp.263-289
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    • 2017
  • The purpose of this study is to enhance the understanding of the provisions for library exceptions in copyright laws and to suggest the direction for the revision of provision in the Copyright Act of Korea. This study compares and analyzes the provisions for library exceptions in several countries' copyright law including Korea, Japan, UK, Australia and USA. This study found that the most common issues in library exception provisions are providing reproduction for users, reproduction for preservation, reproduction for interlibrary loan and digitization of library collections but the conditions for each issue are quite different. The suggestions for the revision of the library exceptions in the Copyright Act of Korea are as follows: changing the amount of reproduction of unpublished works, of works which cannot be obtained at a fair price and of periodicals for users, the transmitting digital reproduction to the user under limited conditions, preparing conditions for reproduction for preservation in libraries, preparing the applicable provisions for reproductions for interlibrary loans and for the limitation of works to be digitized and extending their use at the same time.

A study on the domestic labor-with emphasis on the reproduction of labor power (가사노동의 성격에 대한 연구-노동력재생산을 중심으로)

  • 홍승아
    • Journal of Families and Better Life
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    • v.16 no.2
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    • pp.11-22
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    • 1998
  • The purpose of this study is to contribute to the theoretical understanding of the nature of domestic labor in relation to the capitalist economic system. In this study I adopted the reproduction of labor for the analytical term. Also I owed theoretical basis to the political economy to analyze the relation between domestic labor and capitalist economy. The family is a very important place which functions to maintain and change both the present and future labor what is called the reproduction of labor power. It also has a close relationship with the total society. The reproduction of labor power is divided by 'gender division of labor' which allocates the responsibility to one gender(women) and exempts it to another gender(men). In conclusion the domestic labor is the very basis of capitalist economy and it is also the preliminary condition to its system. SO we can fully understand the capitalist economy with the 'visible' labor part and the 'invisible' domestic labor part inc usively. And we can also understand the interrelationship between family and labor market which constitutes one economic system and fuctions in it.

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The influence of trapping on printed color (다색인쇄에서의 Trapping의 영향)

  • JongWonKim
    • Journal of the Korean Graphic Arts Communication Society
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    • v.3 no.1
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    • pp.1-10
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    • 1985
  • In Graphic art reproduction process it is important to realize that which set of inks and their densities (film thickness) and which set of halftone positive should be aimed at. in order to meet the demand for good color reproduction. There have been various studies to calculation the color reproduction of the graphic arts. however. these study were based on the assumed characteristics of the materials. In this study the relationship between wet-trapping (wet on wet-printing) and increases of ink layer and printing sequence are examined.

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A Study on Human Resource Training Plan and Status of the New Reproduction Energy Industry in Gwangju area (광주지역 신재생에너지 산업의 현황과 인력양성방안에 관한 연구임)

  • Yim, Ki-Heung
    • Journal of Digital Convergence
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    • v.8 no.4
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    • pp.47-57
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    • 2010
  • The purpose of this study is to suggest the effective alternative plan of the human resource training in the new reproduction energy industry, here in Gwang-Ju area. Nowadays, we, Korea is confronted with great changing moment to reform the developmental model in social situation & economical paradigms. Based on the aspect, the study showed that how suitable place to carry out the aims in Gwangju area ; first, the good equipped natural environment for activating is apt to build the new reproduction energy industry. And next, the good skilled human resource training is to get into the newly industry. According, the various policy of educating manpower for activating new reproduction energy industry need to be driven in short and long term.

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Estimation of Reproduction Number for COVID-19 in Korea (국내 코로나바이러스감염증-19의 감염재생산수 추정)

  • Jeong, Jaewoong;Kwon, Hyuck Moo;Hong, Sung Hoon;Lee, Min Koo
    • Journal of Korean Society for Quality Management
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    • v.48 no.3
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    • pp.493-510
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    • 2020
  • Purpose: As of July 31, there were 14,336 confirmed cases of COVID-19 in South Korea, including 301 deaths. Since the daily confirmed number of cases hit 909 on February 29, the spread of the disease had gradually decreased due to the active implementation of preventive control interventions, and the daily confirmed number had finally recorded a single digit on April 19. Since May, however, the disease has re-emerged and retaining after June. In order to eradicate the disease, it is necessary to suggest suitable forward preventive strategies by predicting future infectivity of the disease based on the cases so far. Therefore, in this study, we aim to evaluate the transmission potential of the disease in early phases by estimating basic reproduction number and assess the preventive control measures through effective reproduction number. Methods: We used publicly available cases and deaths data regarding COVID-19 in South Korea as of July 31. Using ensemble model integrated stochastic linear birth model and deterministic linear growth model, the basic reproduction number and the effective reproduction number were estimated. Results: Estimated basic reproduction number is 3.1 (95% CI: 3.0-3.2). Effective reproduction number was the highest with 7 on February 15, decreased as of April 20. Since then, the value is gradually increased to more than unity. Conclusion: Preventive policy such as wearing a mask and physical distancing campaigns in the early phase of the outbreak was fairly implemented. However, the infection potential increased due to weakening government policy on May 6. Our results suggest that it seems necessary to implement a stronger policy than the current level.

CO-CULTURE OF BOVINE EMBRYOS WITH CUMULUS CELLS

  • Goto, K.;Koba, M.;Takuma, Y.;Nakanishi, Y.;Ogawa, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.2 no.4
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    • pp.595-598
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    • 1989
  • Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of culture, respectively, while embryos/ova cultured in medium alone failed to develop beyond 8-cell (0-13.3%), morula (0-1.5%) and blastocyst stages (0%). The results of this study demonstrated the beneficial effect of cumulus cells on the development of bovine embryos.