• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.3
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• pp.92-102
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• 2022

Various numerical analysis models can be used to evaluate the behavior characteristics of tunnel facilities which are representative underground structures. In general, the Mohr-Coulomb model, which is most often used for numerical analysis, is an elastic-perfect plastic behavior model. And the deformation characteristics are the same during the load increase-load reduction phase. So there is a problem that the displacement may appear different from the field situation in the case of excavation analysis. In contrast, the HS-small strain stability model has a wide range of applications for each ground. And it is known that soil deformation characteristics can be analyzed according to field conditions by enabling input of initial elastic modulus and nonlinear curve parameter and so on. However, civil engineers are having difficulty using nonlinear models that can apply material nonlinear properties due to difficulties in estimating ground property coefficients. In this study, the necessity of rational model selection was reviewed by comparing the results of seismic performance evaluation using the Mohr-Coulomb model, which civil engineers generally apply for numerical analysis of tunnels, and the HS Small strain Stiffness model, which can consider ground nonlinearity.

• Journal of fish pathology
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• v.1 no.1
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• pp.45-50
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• 1988

26 strains of Vibrio anguillarum isolated from Seriola quinqueradiata, Chrysophrys major and Oplegnathus faseiatus between July 1985 and December 1986 were examined for their serological identification. When the isolates were examined on the basis of cross-agglutination and crossabsorption tests with thermostable antigens(O) of V, anguillarum 6 serotypes reported by Kitao et at.(1983), they showed positive agglutination reaction only with serotype C, the representative strain PT-213, and also formed double precipitation lines against anti-PT-213 serum, and specifically reacted with the PT-213 strain on cross-absorption test. Therfore, it was thought that all the isolates have single serotype and its serotype is identical with serotype C. the representatve strain PT-213 reported by Kitao et al. (1983).

• Tunnel and Underground Space
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• v.19 no.2
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• pp.158-166
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• 2009

As a basic study for investigating the development of the stress-induced crack in Hoek-Brown rock, a homogenization technique of elastic cracks is proposed. The onset of crack is monitored by Hoek-Brown empirical criterion, while the orientation of the crack is determined by the critical plane approach. The concept of volume averaging in stress and strain component was invoked to homogenize the representative rock volume which consists of intact rock and cracks. The formulation results in the constitutive relations for the homogenized equivalent anisotropic material. The homogenization model was implemented in the standard FEM code COSMOSM. The numerical uniaxial tests were performed under plane strain condition to check the validity of the propose numerical model. The effect of friction between the loading plate and the rock sample on the mode of deformation and fracturing was examined by assuming two different contact conditions. The numerical simulation revealed that the homogenized model is able to capture the salient features of deformation and fracturing which are observed commonly in the uniaxial compression test.

• The Plant Pathology Journal
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• v.28 no.4
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• pp.423-427
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• 2012

Pseudomonas syringae pv. actinidiae strains, the causal agents of bacterial canker on kiwifruit, were isolated from Korea and Italy in 2011. Among 87 isolates, a total of six representative strains, three from Korea and three from Italy, were identified on the basis of biochemical and physiological tests. Identities were confirmed by PCR using P. syringae pv. actinidiae-specific primers PsaF1/R2, which amplified a 280-bp DNA fragment. The strains isolated from Korea in this study displayed BOX-PCR patterns similar to those isolated from Italy but different from those isolated previously in Korea or the pathotype P. syringae pv. actinidiae strain. The effector hopA1 and hopH1 genes, which are known to be present in strains isolated recently from France and Italy, were also present in P. syringae pv. actinidiae strains, SYS1, SYS2 and SYS4, isolated from Korea in this work. However, no amplicons of the expected size were obtained from strains previously isolated from Korea and Japan. In addition, the Korean strains isolated in this work belonged to haplotype I for the cts gene identical to those strains isolated from recent outbreaks in Italy. These results suggest that P. syringae pv. actinidiae strains isolated from Korea and examined in this work are a new type of strain similar to those found from recent outbreaks in Italy. This is the first report on the occurrence of cts haplotype I strains of P. syringae pv. actinidiae affecting kiwifruit plants in Korea.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.105-110
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• 1997

Mixture study of bifenthrin and prothiofos was conducted to control insecticide resistant diamondback moth (DBM), Plutella xylostella, which had shown 581, 18, 19, 11 fold resistance to fenvalerate, cypermethrin, furathiocarb, and prothiofos in Chinju strain, respectively and 38 and 9 fold resistance to fenvalerate and furathiocarb in Seosang strain, respectively. Optimal mixture ratios of bifenthrin and prothiofos was selected against Seosang strain of DBM by leaf dipping method in laboratory and by field test. Maximum co-toxicity coefficient by dipping method was shown at a ratio of 1:50 mixture of bifenthrin 1EC and prothiofos 50EC in active ingredient(a.i.) by 243.2 and then suddenly decreased. The mixed formulation from the dipping test was examined at the cabbage field of Seosang, Hamyang. Although insecticides were reduced to half, control efficacy was similar to farmer's conventional method. In addition, the mixture combination was also more effective against Chinju DBM population which had very high level of resistance to several representative insecticides. Mixture methods of insecticides will be effective countermeasures to the resistance problem of pests.

• Journal of Microbiology and Biotechnology
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• v.31 no.3
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• pp.398-407
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• 2021

Using salt-tolerant bacteria to protect plants from salt stress is a promising microbiological treatment strategy for saline-alkali soil improvement. Here, we conducted research on the growth-promoting effect of Brevibacterium frigoritolerans on wheat under salt stress, which has rarely been addressed before. The synergistic effect of B. frigoritolerans combined with representative salt-tolerant bacteria Bacillus velezensis and Bacillus thuringiensis to promote the development of wheat under salt stress was also further studied. Our approach involved two steps: investigation of the plant growth-promoting traits of each strain at six salt stress levels (0, 2, 4, 6, 8, and 10%); examination of the effects of the strains (single or in combination) inoculated on wheat in different salt stress conditions (0, 50, 100, 200, 300, and 400 mM). The experiment of plant growth-promoting traits indicated that among three strains, B. frigoritolerans had the most potential for promoting wheat parameters. In single-strain inoculation, B. frigoritolerans showed the best performance of plant growth promotion. Moreover, a pot experiment proved that the plant growth-promoting potential of co-inoculation with three strains on wheat is better than single-strain inoculation under salt stress condition. Up to now, this is the first report suggesting that B. frigoritolerans has the potential to promote wheat growth under salt stress, especially combined with B. velezensis and B. thuringiensis.

• Journal of Microbiology and Biotechnology
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• v.33 no.4
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• pp.519-526
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• 2023

Panax ginseng is one of the most important herbal medicinal plants consumed as health functional food and can be fermented to achieve better efficacy. Lacticaseibacillus, one of the representative genera among lactic acid bacteria (LAB), has also been used as a probiotic material for health functional foods due to its beneficial effects on the human body. To achieve a synergistic effect by using these excellent dietary supplement ingredients together, a novel LAB strain was isolated from the root of 6-year-old ginseng. Through similarity analysis of 16S rRNAs and whole-genome sequences, the strain was confirmed as belonging to the genus Lacticaseibacillus and was named L. casei KGC1201. KGC1201 not only met all safety standards as food, but also showed excellent probiotic properties such as acid resistance, bile salt resistance, and intestinal adhesion. In particular, KGC1201 exhibited superior acid resistance through morphological observation identifying that the cell surface damage of KGC1201 was less than that of the L. casei type strain KCTC3109. Gene expression studies were conducted to elucidate the molecular mechanisms of KGC1201's acid resistance, and the expression of the glycosyltransferase gene was found to be significantly elevated under acidic conditions. Exopolysaccharides (EPSs) biosynthesized by glycosyltransferase were also increased in KGC1201 compared to KCTC3109, which may contribute to better protection of KGC1201 cells from strong acidity. Therefore, KGC1201, with its increased acid resistance through molecular mechanisms and excellent probiotic properties, can be used in health functional foods to provide greater benefit to overall human health and well-being.

• Journal of Microbiology and Biotechnology
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• v.34 no.9
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• pp.1826-1835
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• 2024

Paracoccus denitrificans has been identified as a representative strain with heterotrophic nitrificationaerobic denitrification capabilities (HN-AD), and demonstrates strong denitrification proficiency. Previously, we isolated the DYTN-1 strain from activated sludge, and it has showcased remarkable nitrogen removal abilities and genetic editability, which positions P. denitrificans DYTN-1 as a promising chassis cell for synthetic biology engineering, with versatile pollutant degradation capabilities. However, the strain's low stability in plasmid conjugation transfer efficiency (PCTE) hampers gene editing efficacy, and is attributed to its restriction modification system (R-M system). To overcome this limitation, we characterized the R-M system in P. denitrificans DYTN-1 and identified a DNA endonuclease and 13 DNA methylases, with the DNA endonuclease identified as HNH endonuclease. Subsequently, we developed a plasmid artificial modification approach to enhance conjugation transfer efficiency, which resulted in a remarkable 44-fold improvement in single colony production. This was accompanied by an increase in the frequency of positive colonies from 33.3% to 100%. Simultaneously, we cloned, expressed, and characterized the speculative HNH endonuclease capable of degrading unmethylated DNA at 30℃ without specific cutting site preference. Notably, the impact of DNA methylase M9 modification on the plasmid was discovered, significantly impeding the cutting efficiency of the HNH endonuclease. This revelation unveils a novel R-M system in P. denitrificans and sheds light on protective mechanisms employed against exogenous DNA invasion. These findings pave the way for future engineering endeavors aimed at enhancing the DNA editability of P. denitrificans.

• Advances in aircraft and spacecraft science
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• v.5 no.2
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• pp.239-249
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• 2018

Physical tests are performed at various stages of the development cycle of a product, from prototype validation to product qualification. Although costly, there are growing demands for qualification tests like endurance vibration testing to be more representative of the real world. At the same time there are growing demands to assess the durability of these items based on FEA simulation. In this paper, we will explain how to set up a CAE-based test and how to correlate the results with some physical measurements. Specific assumptions will be explained and some advantages of using virtual tests will be highlighted such as the reduction of the number of prototypes needed, investigations on failures, evaluation of the level of reliability via sensitivity analysis, evaluation of the margins are at the end of a successful test. This presentation will therefore focus on explaining and showing how virtual tests can enrich the exploitation of physical tests.

• Journal of Mechanical Science and Technology
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• v.18 no.8
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• pp.1327-1337
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• 2004

An efficient multi-objective optimization method is presented making use of neural network and a systematic satisficing trade-off method (STOM), in order to simultaneously improve both maneuverability and durability of tire. Objective functions are defined as follows: the sidewall-carcass tension distribution for the former performance while the belt-edge strain energy density for the latter. A back-propagation neural network model approximates the objective functions to reduce the total CPU time required for the sensitivity analysis using finite difference scheme. The satisficing trade-off process between the objective functions showing the remarkably conflicting trends each other is systematically carried out according to our aspiration-level adjustment procedure. The optimization procedure presented is illustrated through the optimum design simulation of a representative automobile tire. The assessment of its numerical merit as well as the optimization results is also presented.