• Journal of the Korean Society for Precision Engineering
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• v.25 no.1
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• pp.145-150
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• 2008

One of the unresolved questions in articular cartilage biomechanics is the magnitude of the dynamic modulus and tissue compressive strains under physiological loading conditions. The objective of this study was to characterize the dynamic modulus and compressive strain magnitudes of bovine articular cartilage at physiological compressive stress level and loading frequency. Four bovine calf shoulder joints (ages 2-4 months) were loaded in Instron testing system under load control, with a load amplitude up to 800 N and loading frequency of 1 Hz, resulting in peak engineering stress amplitude of ${\sim}5.8\;MPa$. The corresponding peak deformation of the articular layer reached ${\sim}27%$ of its thickness. The effective dynamic modulus determined from the slope of stress versus strain curve was ${\sim}23\;MPa$, and the phase angle difference between the applied stress and measured strain which is equivalent to the area of the hystresis loop in the stress-strain response was ${\sim}8.3^{\circ}$. These results are representative of the functional properties of articular cartilage in a physiological loading environment. This study provides novel experimental findings on the physiological strain magnitudes and dynamic modulus achieved in intact articular layers under cyclical loading conditions.

• Korean Journal of Microbiology
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• v.23 no.4
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• pp.282-290
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• 1985

Small size antibiotic resistance plasmids having molecular weights less than 10 Mdal were isolated and characterized from ten clinically isolated multiple resistant Staphylococcus aureus. Agarose gel electrophoresis profiles and antibiotic resistance patterns divided these strains into four groups. Strain 2-23-6, the representative strain of a group of five strains conferred two plasmids of molecular weights $1.6{\times}10^6\;dal\;and\;2.0{\times}10^6$ dal. The small plasmid (pSBK 112) specified macrolides, lincosamides and streptogramin type B (MLS) resistance gene which are expressed constitutively. Lage plasmid (pSBK 125) specified chloramphenicol resistance gene which is inducible. Strain 10-5 conferred a $3.0{\times}10^6$ dal plasmid (pSBK 141) which carry an inducible ampicillin resistance gene and strain P-H-2 conferred and $1.6{\times}10^6$ dal plasmid (pSBK 190) which carry a constitutive MLS resistance gene. Strain D-H-1 conferred four plasmids of molecular weights $0.8{\times}10^6$ dal (pSBK 201), $1.6{\times}10^6$ dal (pSBK 202), $2.5{\times}10^6$ dal (pSBK 203), and $1.2{\times}10^7$ dal (pDBK 204), respectively. Among those four plasmids, only pSBK 203 specified chloramphenicol resistance gene. Curing of constitutive MLS resistance using acriding orange or ethidium bromide in 2-23-6 and P-H-2 strains produced 'inducible' MLS resistance strains which are less resistant to MLS than the wild type strains, suggesting that there are two resistance genes in both strains; one is constitutive and the other is inducible.

• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.194-197
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• 2021

Salmonella enterica is a representative foodborne pathogen in the world. The S. enterica strain K_SA184 was isolated from the lamb (Ovis aries), which was collected from a local traditional market in South Korea. In this study, the S. enterica strain K_SA184 was sequenced using PacBio RS II and Illumina NextSeq 500 platforms. The final complete genome of the S. enterica strain K_SA184 consist of one circular chromosome (4,725,087 bp) with 52.3% of guanine + cytosine (G + C) content, 4,363 of coding sequence (CDS), 85 of tRNA, and 22 of rRNA genes. The S. enterica strain K_SA184 genome includes encoding virulence genes, such as Type III secretion systems and multidrug resistance related genes.

• Journal of Ocean Engineering and Technology
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• v.17 no.5 s.54
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• pp.66-75
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• 2003

Long cylinder, subjected to internal pressure, is important in the analysis and design of nuclear fuel rod structures. In many cases, long cylinder problems have been considered as a plane strain condition. However, strictly speaking, long cylinder problems are not plane strain problems, but rather a general plane strain (GPS) condition, which is a combination of a plane strain state and a uniform strain state. The magnitude of the uniform axial strain is required, in order to make the summation of the axial force zero. Although there has been the GPS element, this paper proposes a general technique to solve long cylinder problems, using several pseudo-general plane strain (PGPS) elements. The conventional GPS elements and PGPS elements employed are as follows: axisymmetric GPS element (GA3), axisymmetric PGPS element (PGA8/6), 2-D GPS element (GIO), 3-D PGPS element (PG20/16), and reduced PGPS elements (RPGA6, RPG20/16). In particular, PGPS elements (PGA8/6, PG20/16) can be applied in periodic structure problems. These finite elements are tested, using several kinds of examples, thereby confirming the validity of the proposed finite element models.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.7
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• pp.977-985
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• 2004

An evaluation of fatigue life of welded components is complicated due to various geometrically complex welding details and stress raisers in vicinity of weld beads, ego under cuts, overlaps and blow holes. These factors have a considerable influence on the fatigue strength of welded joints, as well as the welding residual stress which is relaxed depending on the distribution of local stress at the front of the stress raisers. To reasonably evaluate fatigue life, the effect of geometries and welding residual stress should be taken into account. The several methods based on the notch strain approach have been proposed in order to accomplish this. These methods, however, result in differences between analytical and experimental results due to discrepancies in estimated amount of relaxed welding residual stress present. In this paper, an approach that involves the use of a modified notch strain approach considering geometrical effects and a residual stress relaxation model based on experimental results was proposed. The fatigue life for five types of representative welding details, ego cruciform, cover plate, longitudinal stiffener, gusset and side attachment joint, are evaluated using this method.

• The Plant Pathology Journal
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• v.19 no.6
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• pp.294-300
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• 2003

Bacterial strains were isolated from diseased samples of shoot blight collected from different pear growing orchards of Chuncheon, Korea from 1995 to 1998. Forty-nine strains showed their pathogenicity on immature fruit and shoot of pear. Microbiological, physiological, and biochemical tests were performed on these pathogenic strains. One strain, designated as WT3 in this study, was selected as a representative strain as it was collected from the first outbreak area in Jichonri, Chuncheon in 1995. Further detailed characterization of the strain WT3 was done by PCR amplification using specific primers described previously for distinguishing Erwinia pyrifoliae from its close pathogen Erwinia amylovora. Based on phenotypical, biochemical, and molecular analyses, strain WT3 was identified as a shoot blight pathogen which was the same as E. pyrifoliae Ep16 previously described by a German group in 1999.

• Geomechanics and Engineering
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• v.24 no.3
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• pp.295-305
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• 2021

In this study, design charts for estimating consolidation settlement are proposed according to finite strain consolidation theory using a nonlinear constitutive relationship equation. Results of parametric sensitivity analysis shows that the final settlement, initial height, and initial void ratio exerted the greatest effect, and the coefficients of the void ratio-effective-stress. Proposed design charts were analyzed for three regions using a representative constitutive relationship equation that enables major dredged-reclaimed construction sites in Korea. The regional design charts can be calculated accurately for the final settlement because it is applied directly to the numerical analysis results, except for reading errors. A general design chart applicable to all marine clays is proposed through correlation analysis of the main parameters. A final self-weight consolidation settlement with various initial void ratios and initial height conditions should be estimated easily using the general design chart and constitutive relationship. The estimated final settlement using the general design chart is similar to the results of numerical analysis obtained using finite strain consolidation theory. Under an overburden pressure condition, design charts for estimating consolidation settlement are proposed for three regions in Korea.

• Microbiology and Biotechnology Letters
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• v.50 no.4
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• pp.465-476
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• 2022

The importance of beneficial microorganisms, particularly probiotics, that coexist in the human body, is being increasingly recognized. Probiotics are representative health functional foods that provide health benefits to humans through the production of various metabolites, including short-chain fatty acids. However, the health benefits are strain-specific, and the use of each probiotic strain should follow guidelines that assure its safety. Accurate identification of the strain should be managed through genetic and phenotypic analyses of the strain. Besides, the functionality of probiotics should be disclosed in vitro and in vivo so that they can be used as legal functional ingredients (i.e., individual standards). In this review, we deal with the guidelines, including the technical factors related to probiotic strains. The common health effects of probiotic strains include proliferation of beneficial bacteria, control of harmful bacteria, and facilitation of bowel activities. Probiotics with various functionalities (e.g., body fat and cholesterol reduction, vaginal health, and improvement of skin's immune system) have been investigated as "individual standards of raw materials for health functional foods" provided by MFDS. In the future, various biotechnologies including synthetic biology can be applied to produce customized probiotics to improve human health.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.41 no.1
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• pp.17-24
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• 2013

In this paper, a micro-mechanical failure prediction program is developed based on SIFT (Strain Invariant Failure Theory) by using the multi-scale modeling method for fiber-reinforced composite materials. And the failure analysis are performed for open-hole composite laminate specimen in order to verify the developed program. First of all, the critical strain invariants are obtained through the tensile tests for three types of specimens. Also, the matrices of strain amplification factors are determined through the finite element analysis for micro-mechanical model, RVE (Representative Volume Element). Finally, the microscopic failure analysis is performed for the open-hole composite laminate specimen model by applying a failure load obtained from tensile test, and the predicted failure indices are evaluated for verification of the developed program.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.10a
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• pp.259-262
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• 2008

This paper introduces a simplified method to consider forming effects in a car crash analysis. Representative value was used to consider forming effects simply. Four representative values, which are the mean value of thicknesses and effective plastic strains at nodes, the median of thicknesses and effective plastic strains at nodes, were evaluated. A crash analysis of a front side member shows that analysis results from the suggested methods are similar to those from the conventional method to consider forming effects. Use of the mean effective plastic strain shows the best results. A car crash analysis for a ULSAB/AVC model under the condition of US SINCAP were carried out to demonstrate the validity of the suggested method. Analysis results show that the error of suggested method is less than 1.5%.