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http://dx.doi.org/10.5423/PPJ.2003.19.6.294

Erwinia pyrifoliae, a Causal Endemic Pathogen of Shoot Blight of Asian Pear Tree in Korea  

Shrestha, Rosemary (Division of Biological Environment, Kangwon National University)
Koo, Jun-Hak (Division of Biological Environment, Kangwon National University)
Park, Duck-Hwan (Division of Biological Environment, Kangwon National University)
Hwang, In-Gyu (School of Agricultural Biotechnology, Seoul National University)
Hur, Jang-Hyun (Division of Biological Environment, Kangwon National University)
Lim, Chun-Keun (Division of Biological Environment, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.19, no.6, 2003 , pp. 294-300 More about this Journal
Abstract
Bacterial strains were isolated from diseased samples of shoot blight collected from different pear growing orchards of Chuncheon, Korea from 1995 to 1998. Forty-nine strains showed their pathogenicity on immature fruit and shoot of pear. Microbiological, physiological, and biochemical tests were performed on these pathogenic strains. One strain, designated as WT3 in this study, was selected as a representative strain as it was collected from the first outbreak area in Jichonri, Chuncheon in 1995. Further detailed characterization of the strain WT3 was done by PCR amplification using specific primers described previously for distinguishing Erwinia pyrifoliae from its close pathogen Erwinia amylovora. Based on phenotypical, biochemical, and molecular analyses, strain WT3 was identified as a shoot blight pathogen which was the same as E. pyrifoliae Ep16 previously described by a German group in 1999.
Keywords
fire blight; Hwankum; necrogens; phytopathogens; plasmid profiles;
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  • Reference
1 Keane, P. J., Kerr, A and New, P. B. 1970. Crown gall of stone fruit. II. Identification and nomenclature of Agribacterium isolates. Aust. J. Biol. Sci. 23:85-595
2 Kim, W.-S., Garden, L., Rhim, S.-L. and Geider, K. 1999. Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). Intl. J. Sys. Bacteriol. 49:899-906   DOI   ScienceOn
3 McGhee, G. C, Schnabel, E. L., Maxson-Stein, K, Jones, B., Stromberg, V. K, Lacy, G. H. and Jones, A. L. 2002. Relatedness of chromosomal and plasmid DNAs of Erwinia pyrifoliae and Erwinia amylovora. Appl. Environ. Microbiol. 63:4421-4426
4 Perry, S. F. 1995. Freeze drying and cryopreservation of bacteria. pp. 21-30. In: Methods in molecular biology. J. G. Day and M. R. McLellan (eds.) Humana Press Inc
5 Van der Zwet T. and Keil, H. L. 1979. Fire blight: a bacterial disease of rosaceous plants. Agriculture Handbook No. 510, US Government printing office, Washington, DC
6 Weisburg, W. G., Barns, S. M., Pelletier, D. A. and Lane, D. J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703
7 Bereswill, S., Jock, S., Bellemann, P. and Geider, K 1998. Identification of Erwinia amylovora by growth in the presence of copper sulfate and by capsule staining with lectin. Plant Dis. 82:158-164   DOI   ScienceOn
8 Schaad, N. W., Jones, J. B. and Chun, W. 1988. Laboratory guide for identification of plant pathogenic bacteria. 2$^n^d$ ed. Amer. Phytopath. SOC., MN, USA
9 Kim, W-S., Hildebrand, M., Jock, S. and Geider, K. 2001a. Molecular comparison of pathogenic bacteria from pear treesin Japan and the fire blight pathogen Erwinia amylovora. Microbiology 147:2951-2959   DOI
10 Falkenstein, H., Zeller, W. and Geider, W. 1989. The 29 kb plasmid common in strains of Erwinia amylovora, modulates development of fire blight symptoms. J. Gen. Microbiol. 135:2643-2650
11 Bereswill, S., Pahl, A., Bellemann, P., Zeller, W. and Geider, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Appl. Environ. Microbiol. 58:3522-3526
12 Nakagawa, T., Shimada, M., Mukai, H., Asada, K., Kato, I., Fujino, K. and Sato, T. 1994. Detection of alcohol-tolerant Hiochi bacteria by PCR. Appl. Environ. Microbiol. 60:637-640
13 Sambrook, J. and Russell, D. W. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor, NY, U.S.A
14 Kim, W-S., Jock, S., Paulin, J.-P., Rhim, S.-L. and Geider, K. 2001b. Molecular detection and differentiation of Erwinia pyrifoliae and host range analysis of the Asian pear pathogen. Plant Dis. 85:1183-1188   DOI   ScienceOn
15 Shrestha, R, Hur, J. H. and Lim, C. K 2001. The effects of temperature and pH on the growth of Asian pear pathogen, Erwinia pyrifoliae. (abstr.) Phytopathology. 91(suppl.):S82
16 Rhim, S.-L., Voelkisch, B., Gardan, L., Paulin, J.-P., Langlotz, C., Kim, W.-S. and Geider, K. 1999. An Erwinia species, different from E. amylovora cause a necrotic disease of Asian pear trees. Plant Pathol. 48:514-520   DOI