• Title/Summary/Keyword: Reporter

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ESTROGENIC ACTIVITIES OF HYDROLYZED AND UNHYDROLYZED EDIBLE PLANT EXTRACTS

  • Kim, Il-Rang;Sheen, Yhun-Yhong;Kwon, Hoon-Jeong
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.164-164
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    • 2002

  • The estrogenic activity of 47 plant extracts was assessed by reporter gene assay using MCF-7 breast cancer cell lines stably transfected with luciferase reporter gene. The estrogenic activity of food extracts was expressed as 17${\beta}$-estradiol(E2) equivalent concentration(EEQ), the concentration of E2 that resulted in the same relative luciferase unit(RLU) of the food extract of 0.2mg/$m\ell$.(omitted)

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Histone deacetylation effects of the CYP1A1 promoter activity, proliferation and apoptosis of cells in hepatic, prostate and breast cancer cells

  • K.N. Min;K.E. Joung;M.J. Cho;J.Y. An;Kim, D.K.;Y.Y. Sheen
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.91-91
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    • 2003

  • We have studied the mechanism of action of TCDD on CYP1A1 promoter activity in both Hepa I and MCF-7 cells using transient transfection system with plAl-Luc reporter gene. When HDAC inhibitors, such as trichostatin A, HC toxin and a novel HDAC inhibitor, IN2001 were cotreated with TCDD to the cells transfected with plAl-Luc reporter gene, the basal promoter activity of CYP1A1 was increased by HDAC inhibitors. Also, in MCF-7 human breast cancer cells, HDAC inhibitors, such as IN2001 and trichostatin A increased the basal activity of CYP1A1 promoter but TCDD stimulated CYP1A1 promoter activity was not changed by HDAC inhibitors. And, in stably-transfected Hepa I cells with plAl-Luc, HDAC inhibitors increased the basal promoter activity only.

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A Study on the influence of journalist's Role-Cognition and Role-Orientation Upon Real Reportage attitude -Focus in editors of National Newspaper (사실적 보도태도에 미치는 기자의 역할인식과 역할지향의 영향력 연구 - 중앙일간지 편집기자를 중심으로)

  • Kang, Hyun Jig
    • Journal of Digital Convergence
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    • v.12 no.9
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    • pp.101-109
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    • 2014

  • The purpose of this study was to analyze the impact of mediator effect related to role-orientation of traditional newspaper editors in terms of role-cognition and role-orientation in aspects of real reportage attitude by classifying the newspaper editor's role-cognition with social stability, reform, check, on the other hand, role-orientation with reporter's intention, intention of actual fact, and reader's intention. The results were as follows: In terms of reporter's intention the research showed the fact that reporter's intentions was influenced by the role cognition of social stability and at the same time, it also made to reduce the attitude of real reportage. Compared with intention of actual fact and reader's intention, the intention of actual fact could be only influenced by the social stability, but it could not give any effectiveness to the attitude of real reportage, furthermore, the research showed the fact that reader's intention aroused some mediator effects which newspaper editor's role cognition gave rise to the attitude of real reportage much more strongly.

  • https://doi.org/10.14400/JDC.2014.12.9.101 인용 PDF KSCI

Bioassays of Polycyclic Aromatic Hydrocarbons using CYP1A1-luciferase Reporter Gene Expression System in Human Breast Cancer MCF-7 Cells

  • Kim, Ja-Y.;Sheen, Yhun-Y.
    • Environmental Mutagens and Carcinogens
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    • v.23 no.2
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    • pp.45-50
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    • 2003

  • Biological activities of PAHs are not known although PAHs are considered as carcinogens. Recent industrial society has human widely exposed to PAHs (polynuclear aromatic hydrocarbons) that are comming from the incomplete combustion of organic material as wider spread environmental contaminants. Our laboratory have been studied the effect of PAHs in the human breast cancer MCF-7 cells. In this study, we examined the human breast cancer MCF-7 cells as a new system to evaluate bioactivity of PAHs. We have selected 13 PAHs to examine bioassay using CYP1A1-luciferase reporter gene expression system where CYP1A1 1.6 Kb 5flanking region DNA was cloned in front of luciferase reporter gene and this plasmid was transfected into MCF-7 cells transiently. This cells then used for the study to observe the effect of PAHs. We demonstrated that PAHs induced the CYP1A1 promoter, CYP1A1 mRNA and 7-ethoxyresolufin O-deethylase (EROD) activities in a concentration-dependant manner. None of PAHs that we have tested showed stronger stimulatory effect on CYP1 gene expression than TCDD. Benz(a)anthracene and benzo(b)fluoranthene were weak responders to CYP1A1 promoter activity stimulation, CYP1A1 mRNA and EROD induction in MCF-7 cells and these chemicals seemed to respond less either CYP1A1 mRNA or EROD than CYP1A1 promoter activity. Benzo(k)fluoranthene, chrysene, and dibenzo(a, h)anthracene showed strong response to CYP1A1 promoter activity stimulation, CYP1A1 mRNA increase and also EROD induction in MCF-7 cells. Results of dose response study suggested that two strong responding PAHs, such as benzo(k)fluoranthene and dibenzo(a, h)anthracene might be mediated through Aryl hydrocarbon receptors system in MCF-7 cells.

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