Proceedings of the Korean Society of Applied Pharmacology (한국응용약물학회:학술대회논문집)
- 2003.11a
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- Pages.91-91
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- 2003
Histone deacetylation effects of the CYP1A1 promoter activity, proliferation and apoptosis of cells in hepatic, prostate and breast cancer cells
- K.N. Min (Ewha Woman′s University, College of Pharmacy) ;
- K.E. Joung (Ewha Woman′s University, College of Pharmacy) ;
- M.J. Cho (Ewha Woman′s University, College of Pharmacy) ;
- J.Y. An (Ewha Woman′s University, College of Pharmacy) ;
- Kim, D.K. (Ewha Woman′s University, College of Pharmacy) ;
- Y.Y. Sheen (Ewha Woman′s University, College of Pharmacy)
- Published : 2003.11.01
Abstract
We have studied the mechanism of action of TCDD on CYP1A1 promoter activity in both Hepa I and MCF-7 cells using transient transfection system with plAl-Luc reporter gene. When HDAC inhibitors, such as trichostatin A, HC toxin and a novel HDAC inhibitor, IN2001 were cotreated with TCDD to the cells transfected with plAl-Luc reporter gene, the basal promoter activity of CYP1A1 was increased by HDAC inhibitors. Also, in MCF-7 human breast cancer cells, HDAC inhibitors, such as IN2001 and trichostatin A increased the basal activity of CYP1A1 promoter but TCDD stimulated CYP1A1 promoter activity was not changed by HDAC inhibitors. And, in stably-transfected Hepa I cells with plAl-Luc, HDAC inhibitors increased the basal promoter activity only.
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