• 한국식품과학회지
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• 제37권6호
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• pp.1005-1011
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• 2005

본 연구에서는 높은 분리능을 가지고 있을 뿐만 아니라 실험의 재현성과 경제성의 측면에서도 많은 장점을 갖고 있는 ERIC DNA sequence를 응용한 ERIC-PCR을 이용하여 Salmonella, E. coli, Shigella, Vibrio 등 4속의 주요 그람 음성 식중독유발 세균들의 분리 동정 방법을 확립하고자 하였다. ERIC-PCR 결과, E. coli의 경우 0.3kb, 0.42kb 및 1.2kb의 band가 모든 균주에서 공통적으로 확인되었고, Salmonella속으로부터는 0.22kb, 0.4kb 및 0.7kb의 band가 증폭되었다. Shigella속은 모든 표준균주와 분리균주로부터 0.33kb와 1.25kb의 band가 증폭되었으며, S. sonnei의 경우 위의 주요 2개 band 이외에도 대부분의 균주에서 0.44kb, 2.0kb 및 3.05kb의 band가 증폭되어 다른 종의 Shigella와 구별되는 fingerprinting pattern을 나타내었다. 그리고 V. parahaemolyticus의 경우 표준균주와 분리균주 모두 0.51kb와 1.5kb의 band가 증폭되어 V. cholerae, V. mimicus 등과 같은 다른 종의 Vibrio와 구별되는 fingerprinting pattern을 나타내었다. 이와 같이 4속의 모든 식중독 균주마다 ERIC-PCR후 생성되는 fingerprinting pattern에서 3-5개의 공통적인 band가 증폭되는 것이 확인되어 이를 이용한 속 수준의 분리 동정과 이러한 주요 band들 이외의 부수적인 band들을 고려하여 종 수준까지의 분리도 가능함을 확인하였다. 따라서 본 연구의 결과는 ERIC 반복적 DNA 염기서열을 이용한 ERIC-PCR이 식중독균의 분리 동정 방법으로 사용될 수 있음을 확인하였으며, 나아가 더 많은 속(genus)의 식중독세균을 대상으로 한 새로운 분리 동정 방법을 확립하는데도 응용이 될 수 있을 것이다.

• 복식
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• 제67권3호
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• pp.81-98
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• 2017

The purpose of this study is to provide basic information for the development of pants patterns with a high level of fit and comfort through calculating surface variations of lower body parts by movement, grouping them into factors, and analyzing how their surface variations link to one another. The achieved results will help determine essential elements for constructing pants patterns, such as key measurements of lower body parts, the amount of ease values and selection of fabrics, which should be taken into consideration for allowing better movement in clothing. The study required lower body 3D scanning of women for analysis, and 13 women between the ages of 20-24 participated in the scanning, which was done by using Artec Eva 3D scanner. Their scanned data were digitalized and converted to measure the values of their lower body surface length and girth in pre-determined positions such as walking, stair climbing and sitting on a chair. These measurements have been statistically analyzed through SPSS 21.0 to obtain the average amounts and rates of extension for each of the measurement item. Some of the highlighted study results are as follows: The surface length and girth measurements were grouped into 4 factors based on their average extension rates. The results from correlation analysis between measurement items within each factor demonstrated that common items linked to all the changes in the values of other items in the three movements. But in most cases, items were not always correlated with each other for different movements. The results also showed that there were correlations between girth measurements, length measurements, and girth and length measurements. Therefore, key measurements for daily pants should be determined within reasonable estimations between relevant measurement items, while the measurements for work pants, which often withstand certain postures or repetitive movements, may require measurement items that are appro priate for, and closely related to, certain movements or tasks.

• 한국패션뷰티학회지
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• 제6권2호
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• pp.97-103
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• 2008

This research thesis is designed to put Korean traditional and unique wedding dress with a greater significance and value than any other dress into application right for the global and contemporary trend, as part of a bid to show Korean traditional beauty. To do it, this thesis has attempted to add the traditional beauty to Korean inherent wedding dress and contemporary wedding dress, with the use of various traditional beauty elements, such as the line firm, materials and color based on the research on wedding dress, such as, 'Wonsam' or 'Unlined Jacket, and 'Long-sleeve Red Silk Jacket', and 'Dangeu' or 'Court Suit' that women wore in the Joseon Dynasty. To recreate Korean traditional beauty of line this research thesis has firstly put into the application the sleeve of 'Wonsam' or 'Unlined Jacket' and the form of a large section of 'Wonsam' of 'Unlined Jacket', and 'Doreyon Line' or 'Trimming Line' of 'Dangeu' or 'Court Suit' as wedding dress that women wore in the Joseon Dynasty. Secondly, this thesis has applied lotus and peony patterns out of traditional ones inlaid with 'Long-Sleeve Silk Jacket' into the contemporary wedding dress to develop a creative and special design. Thirdly, regarding the expressive pattern technique, this thesis has designed the repetitive rhythmical pattern with the use of beading technique and embroidery technique, which makes needle work picture and pattern in fabric. Lastly, it can demonstrate the original and distinct Korean style in application of western wedding dress into traditional and contemporary dress, by devising two collections of dress art pieces.

• 한국실내디자인학회논문집
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• 제24권1호
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• pp.12-22
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• 2015

When it comes to the architecture design, Kuma Kengo rejects a strong and violent subject-centered position and advocates the design that is object-oriented. As can be seen in 'gentle architecture', 'three lows principle', 'natural architecture', and 'connecting architecture', he clearly expresses the objective nature of architecture design in those terms. In this respect, the purpose of this study is to make a close inquiry into the meaning, effect and characteristics of objectivity. In particular, we try to identify the contents of 'impure architecture', which has a clear ambivalence to be an instrumental expression strongly settled in the objectivity, in an aesthetic standpoint. To do that, we systemized the concept of mimesis and the theory of subversive appropriation by Walter Benjamin and Theodor Adorno in to a frame of interpretation. By systemizing translation, subversion, verbalization and the dialectic structure of the aesthetics of negation, we interpreted the features of his works as an objective work and 'impure architecture'. His objectivity leads the situation by subversively appropriating the inherent elements of architectural conditions based on a dialectic solution in which inquiries on logical and scientific materials have played a critical role. Above all, through all these processes, he tried to suggest a language as a new technique for materials and structures. Ultimately, we could find out that this object oriented design sublates a subject oriented way that is monolithic and repetitive regardless of objects. Rather, it is a way that is effective in creating a new way of design by making a different approach to a new object rather unfamiliarly, yet deeply.

• Journal of Periodontal and Implant Science
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• 제44권5호
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• pp.227-234
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• 2014

Purpose: Dentin hypersensitivity is a potential threat to oral health. Laser irradiation may provide reliable and reproducible treatment but remains controversial. The present study aimed to evaluate the effects of $CO_2$ or erbium-doped yttrium aluminium garnet (Er:YAG) laser therapy, and to assess mineral content. Methods: Eighteen human single-rooted teeth affected with advanced periodontitis were obtained. Buccal and lingual surfaces were planed to form 36 specimens. Ethylenediaminetetraacetic acid gel (24%) was applied to remove the smear layer and simulate hypersensitive teeth. The experimental groups were: group 1, control (no irradiation); group 2, $CO_2$ laser (repetitive pulsed mode, 2 W, $2.7J/cm^2$); and group 3, Er:YAG laser (slight contact mode, 40 mJ/pulse and 10 Hz). To evaluate dentinal tubule occlusion, six specimens per group (2-mm thickness) were prepared and observed using scanning electron microscopy (SEM) for calculation of the occlusion percentage. To evaluate the mineral content, six specimens per group (0.6-mm thickness) were used, and then the levels of Ca, K, Mg, Na, and P were measured by inductively coupled plasma-atomic emission spectrometry. In addition, the surface temperature of the specimens during laser irradiation was analyzed by a thermograph. Results: The SEM photomicrographs indicated melted areas around exposed dentinal tubules and a significantly greater percentage of tubular occlusion in the $CO_2$ and Er:YAG laser groups than the control, and in the Er:YAG group than the $CO_2$ laser group. In addition, no significant differences were noted among the experimental groups for the mineral elements analyzed. The $CO_2$ laser group showed an evident thermal effect compared to the Er:YAG group. Conclusions: $CO_2$ and Er:YAG laser are effective in treating dentin hypersensitivity and reducing its symptoms. However, the Er:YAG laser has a more significant effect; thus, it may constitute a useful conditioning item. Furthermore, neither $CO_2$ nor Er:YAG lasers affected the compositional structure of the mineral content.

• 한국광학회지
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• 제28권6호
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• pp.304-313
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• 2017

대구경 거울과 이를 지지하는 flexure로 구성된 반사경 시스템의 최적화를 위하여 수행하는 유한요소 해석은 주어진 설계 조건을 만족하기 위하여 수많은 반복적인 계산과 실제 모델의 수정 작업이 필수적이다. 일반적으로 실제 모델의 수정은 node의 재설정과 새로이 구성된 각 부품의 경계면에서 node의 연속성을 맞추는 작업에 많은 시간이 소요되며 이는 유한요소 해석에 소요되는 시간 결정에 매우 중요한 요소가 된다. 모델링과 계산에 소요되는 시간을 절약하기 위하여 각 광학적 구성요소의 경계면에서 비공유 node 연결을 활용하는 새로운 광기계 해석을 제안하고자 한다. 새 모델링 기법에 의하여 계산된 광기계 해석과 경계면에서의 공유 node를 사용하는 기존의 광기계 해석을 비교하여, 계산에 의하여 얻어진 광기계적 성능은 거의 같았고, 주어진 조건에 도달하기 위한 계산 시간은 획기적으로 줄어드는 것을 확인할 수 있었다.

• 한국실내디자인학회논문집
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• 제16권5호
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• pp.38-46
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• 2007

In the late 1970s, the method of quantitative and scientific space structural analysis based on graph theory was introduced to the process of space design, which arranges design and functional elements, as relying heavily on intuition could produce errors due to unverified experiences and prejudices of the designer. As the method of space analysis is complex and hard to express visually and requires repetitive operations, it was discussed theoretically only. However, with the development of computer performance and graphic in recent years, visualization became possible. But the method of visual structural analysis of space is at the level of two dimensions and it is not easy to get accurate data when it is applied to limited three dimensional space such as an interior space. For the visual structural analysis of space, this study presents 4 indices including visibility volume level, pure visibility connection frequency, effective visibility connection frequency, and path visibility connection frequency. This study also presents space division using three dimensional arrangement rather than the existing vector operation method and raytracing algorithm at the lattice constant. Based on this, an analysis tool for the visible regions of three dimensional space that is capable of evaluating at multiple points by using three dimensional game engine and presentation tool that allows the analyzer to interpret the data effectively is made. It is applied to 2 prototype models by displacing Z axis, and the results are compared with UCL Depthmap to verify the validity of data and evaluate its usefulness as a multidimensional, multi-view space analysis tool.

• 한국건설관리학회논문집
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• 제5권4호
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• pp.122-129
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• 2004

현재 대부분의 리모델링 현장은 시설에 대한 사전정보부족, 공간적인 제약과 같은 예상치 못한 현장조건. 작업범위의 변경 등으로 인해 촉박한 공사기간으로 추진되고 있으며, 이는 조악한 품질의 원인이 되고 있다. 또한 다소 복잡한 작업체계를 가지고 있어 기존시설의 운영일정과 공사일정과의 충돌로 인한 갑작스런 작업대기시간의 발생 등 효율적인 공정관리에도 많은 어려움이 뒤따른다. 따라서 본 연구에서는 실제 리모델링 사례를 통하여 현장관리 절차 및 사례현장에서 나타난 시공관리상 문제점의 개선과정을 통해 리모델링 공사현장의 효율적 관리방안을 제시하는 것을 목적으로 하였다. 관리방안의 수립을 위해 문헌을 통하여 관리상의 문제점을 도출하였고, 사례분석을 통해 공사단계별 현장관리절차, 관리사항을 파악하여 관리상 성공요인을 도출하고, 향후 리모델링 현장에서의 효율적 관리방안을 제시하였다.

• 디지털융복합연구
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• 제16권1호
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• pp.337-342
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• 2018

금속공예 분야에서 점차 쇠퇴되는 대공작품의 예술적 미적 가치를 연구하기 위해 시작된 본 연구는 디자인 요소인 점, 선, 면, 형을 중심으로 한 조형 표현방법으로 선과 면을 선정하고 이를 금속조형으로 시각화 하였다. 선행연구를 통해 알아본 선과 면의 미적 해석은 생명력 있는 반복적 아름다움으로 정의 되었는데 연구자는 여기에서 더 나아가 반복적이면서도 변화를 느낄 수 있는 조형 작품을 나타내고자 했다. 그 결과 선과 면이 규칙적 반복이 아닌 자연스럽게 점진되는 변화를 통해 어려운 기법이나 고도 기술의 제작 방법 없이도 선과 면의 단순한 변화 속에서 느껴지는 미적 아름다움을 표현할 수 있는 탐구의 대상이 가능함을 알 수 있었다. 또한 쇠퇴해가는 금속조형의 고부가가치 산업으로서의 성장을 예측할 수 있었다. 본 연구를 시작으로 향후 선과 면의 활용이 조형요소로서 매우 다양한 미적표현 방법으로 응용될 수 있는 연구가 계속 되기를 바란다.

• Molecules and Cells
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• 제37권6호
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• pp.467-472
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• 2014

Obesity is known to be strongly associated with cardiovascular disease and cancer, the leading causes of mortality worldwide, and develops owing to interactions between genes and the environment. DNA methylation can act as a downstream effector of environmental signals, and analysis of this process therefore holds substantial promise for identifying mechanisms through which genetic and environmental factors jointly contribute to disease risk. Global DNA methylation of peripheral blood cells has recently been proposed as a potential biomarker for disease risk. Repetitive element DNA methylation has been shown to be associated with prominent obesity-related chronic diseases, but little is known about its relationship with weight status. In this study, we quantified the methylation of Alu elements in the peripheral blood DNA of 244 healthy women with a range of body mass indexes (BMIs) using pyrosequencing technology. Among the study participants, certain clinical laboratory parameters, including hemoglobin, serum glutamic oxaloacetic transaminase, serum glutamic- pyruvic transaminase, total cholesterol, and triglyceride levels were found to be strongly associated with BMI. Moreover, a U-shaped association between BMI and Alu methylation was observed, with the lowest methylation levels occurring at BMIs of between 23 and $30kg/m^2$. However, there was no significant association between Alu methylation and age, smoking status, or alcohol consumption. Overall, we identified a differential influence of BMI on global DNA methylation in healthy Korean women, indicating that BMI-related changes in Alu methylation might play a complex role in the etiology and pathogenesis of obesity. Further studies are required to elucidate the mechanisms underlying this relationship.