• Title/Summary/Keyword: Repeated structure

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Denaturation and Inactivation of Antioxidative Enzymes due to Repeated Exposure to UV-B and Inhibitory Effect of RGP Lens (UV-B 반복노출에 따른 항산화효소의 변성 및 활성저하와 RGP렌즈의 차단효과)

  • Byun, Hyun Young;Lee, Eun Jung;Oh, Dae Hwan;Kim, So Ra;Park, Mijung
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.2
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    • pp.237-246
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    • 2015
  • Purpose: The present study was conducted to reveal the correlation of structural denaturation and decrease of enzyme activity when the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT) were repeatedly exposed to UV-B, and further investigate whether the denaturation and inactivation of those enzymes can be effectively blocked by using UV-inhibitory RGP lens. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-B of 312 nm for 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-B irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. At that time, the change in structure and activity of the antioxidant enzymes directly exposed to UV-B was compared to the case that UV-B was blocked by UV-inhibitory RGP lens. Results: SOD exposed repeatedly to UV-B showed the polymerization pattern in the electrophoretic analysis when it repeatedly exposed for 30 min a day, however, the change of its activity was less than 10%. On the other hand, CAT repeatedly exposed to UV-B reduced size and density of the electrophoretic band which indicated a structure denaturation, and its activity was significantly decreased. In the case that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band occurred in the electrphoretic analysis. In addition, the degeneration of CAT due to UV-B irradiation was inhibited to some extent by using RGP lens with a UV-B blocking of 63.7%, however, it was not completely inhibited. Conclusions: From these results, it was revealed that the structural denaturation of antioxidative enzymes was not perfectly correlated with the reduction in enzyme activity according to the type of enzyme. It is recommended to minimize the exposure time to UV when wearing contact lens, or wear the contact lenses having UV blocking rate of the FDA Class I blocker or the sunglasses having equivalent UV-blocking rate for reducing the damage of antioxidative enzymes induced by UV.

Characteristics of Sand-Silt Mixtures during Freezing-Thawing by using Elastic Waves (탄성파를 이용한 모래-실트 혼합토의 동결-융해 특성)

  • Kang, Mingu;Kim, Sangyeob;Hong, Seungseo;Kim, Youngseok;Lee, Jongsub
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.5
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    • pp.47-56
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    • 2014
  • In winter season, the pore water inside the ground freezes and thaws repetitively due to the cold air temperature. When the freezing-thawing processes are repeated on the ground, the change in soil particle structure occurs and thus the damage of the infrastructure may be following. This study was performed in order to investigate the stiffness change of soils due to the freeze-thaw by using elastic waves. Sand-silt mixtures are prepared with in the silt fraction of 40 %, 60 % and 80 % in weight and in the degree of saturation of 40 %. The specimens are placed into the square freezing-thawing cell by the temping method. For the measurement of the elastic waves, a pair of the bender elements and a pair of piezo disk elements are installed on the cell, and a thermocouple is inserted into soils for the measurement of the temperature. The temperature of the mixtures is decreased from $20^{\circ}C$ to $-10^{\circ}C$ during freezing, is maintained at $-20^{\circ}C$ for 18 hours, is gradually increased up to the room temperature of $20^{\circ}C$ to thaw the specimens. The shear waves, the compressional waves and the temperature are measured during the freeze-thaw process. The experimental result indicates that the shear and the compressional wave velocities after thawing are smaller than those of before freezing. The velocity ratio of after thawing to before freezing of shear wave is smaller than that of the compressional wave. As silt fraction increases from 40 % to 80 %, the shear and compressional wave velocities are gradually increased. This study suggests that the freezing-thawing process in unsaturated soil loosens the soil particle structure, and the shear wave velocity reflects the effect of freezing-thawing more sensitively than the compressional wave velocity.

A study on application of fractal structure on graphic design (그래픽 디자인에 있어서 프랙탈 구조의 활용 가능성 연구)

  • Moon, Chul
    • Archives of design research
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    • v.17 no.1
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    • pp.211-220
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    • 2004
  • The Chaos theory of complexity and Fractal theory which became a prominent figure as a new paradigm of natural science should be understood not as whole, and not into separate elements of nature. Fractal Dimensions are used to measure the complexity of objects. We now have ways of measuring things that were traditionally meaningless or impossible to measure. They are capable of describing many irregularly shaped objects including man and nature. It is compatible method of application to express complexity of nature in the dimension of non-fixed number by placing our point of view to lean toward non-linear, diverse, endless time, and complexity when we look at our world. Fractal Dimension allows us to measure the complexity of an object. Having a wide application of fractal geometry and Chaos theory to the art field is the territory of imagination where art and science encounter each other and yet there has not been much research in this area. The formative word has been extracted in this study by analyzing objective data to grasp formative principle and geometric characteristic of (this)distinct figures of Fractals. With this form of research, it is not so much about fractal in mathematics, but the concept of self-similarity and recursiveness, randomness, devices expressed from unspeakable space, and the formative similarity to graphic design are focused in this study. The fractal figures have characteristics in which the structure doesn't change the nature of things of the figure even in the process if repeated infinitely many times, the limit of the process produces is fractal. Almost all fractals are at least partially self-similar. This means that a part of the fractal is identical to the entire fractal itself even if there is an enlargement to infinitesimal. This means any part has all the information to recompose as whole. Based on this scene, the research is intended to examine possibility of analysis of fractals in geometric characteristics in plasticity toward forms in graphic design. As a result, a beautiful proportion appears in graphic design with calculation of mathematic. It should be an appropriate equation to express nature since the fractal dimension allows us to measure the complexity of an object and the Fractla geometry should pick out high addition in value of peculiarity and characteristics in the complex of art and science. At the stage where the necessity of accepting this demand and adapting ourselves to the change is gathering strength is very significant in this research.

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A Study on Orchestration in "Battle for The Glory" out of the Background Music in the Animation "Dragon Quest IV" (애니메이션 "드래곤 퀘스트 IV"의 배경음악 중 "Battle for The Glory"에 나타난 관현악법 연구)

  • Jung, Kil
    • Cartoon and Animation Studies
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    • s.39
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    • pp.321-348
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    • 2015
  • The purpose of this study was to find a system and a progression principle in orchestral piece based on the outcome after comparatively analyzing the orchestral operation technique in "Battle for The Glory" out of the background music in the animation "Dragon Quest IV" by Koichi Sugiyama(1931~), who is a leading runner of Japan's animation music, based on functional parts daccord & Instrumentation Pattern, Rhythm Pattern, Voicing Pattern, and harmonic ratio by progression section devised by the writer. As a result, first, five themes in this music have specific instrumentation pattern, respectively. In a passage that is shown exposition, reprise, and representation in theme, the unity was emphasized by maintaining the same instrumentation pattern. On the contrary to this, a passage of being suggested new theme is being used the exchange method and addition & subtraction in musical instrument in order to strengthen diversity. Second, the voicing pattern is forming the vertical contrasting relationship of "thinness-thickness" on the whole. However, the diversity is being intensified that is changed into the structure of "thickness-thinness" in the third theme and of "thinness-thickness" that has two melodies in the fourth theme. Third, the rhythm pattern is forming the vertical contrasting relationship of "big-small" on the whole. However, the fifth theme is being given diversity with being changed into the structure of "small-big." Fourth, the harmony by progression section from the horizontal perspective is shown to be high in the proportion of unity in the section of being repeated and represented the theme and to be high in the proportion of diversity in the section of being suggested new theme. In this study, the balanced orchestral operation technique through the operation technique, which was used in this work, is what extracts the relationship of diverse proportions in the horizontal progression section based on the technique of vertical perspective. In this aspect, this analytical study is desired to be positioned as a new paradigm in establishing a theoretical system and an educational method in orchestration.

Landscape Composition Based on Placement and Harmony in the Namgea Suhwon (치(置)와 화(和)의 개념으로 분석한 남계서원의 경관짜임)

  • Rho, Jae-Hyun;Shin, Sang-Sup
    • Journal of the Korean Institute of Landscape Architecture
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    • v.37 no.4
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    • pp.72-85
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    • 2009
  • This study attempts to examine the principles of landscape composition for a 'Suhwon(書院)' and the meaning and value of its traditional landscape architecture, in order to apply the results to the design of modern landscape architecture. A 'Suhwon' is a vital space containing the form and meaning of human activity. This study analyzes the characteristics of landscape composition in the construction of the Namgea Suhwon, located in Hamyang, by examining the form and meaning of its area and composition. The Namgea Suhwon was constructed with a suitable configuration and harmony in a good location, neither field nor mountain, and which encompasses transcendence and a return after passage through a period of birth and abundance. Its appearance means 'life existence and hidden death(生居死幽)'. Its spatial system is a reflection of the idea of Samshinoje(三神五帝: The three abilities of Providence and its five subjects) connected with Ilsangje -Samshin -Ohje. It was built based on the idea of Biryebudong(非禮不動) meaning that one should follow only good decorum and avoid discourtesy, complying with "the frame of decorum" developed by the family rites of Chu Hsi. The environmental design of the Namgea Suhwon was interrupted by the material confrontation between mountains and water and a binary code system, such as front to rear, length to breadth, and movement to stillness. The design did not adhere to stiff axes, but pursued the harmonic principles of asymmetric balance in the building and the yard, which are very naturalistic. The name 'Namgea Suhwon' is closely related with the view of placement(置) and harmony(和), which are unified with the function and meaning formed by connecting Sung Confucianism with the Pungsu-Sasinsa structure in the layout of the grounds. When examining the D/H ratio of the building and yard, it can be seen that the spaces of Ganghak, Yusang and Jehyang were built appropriately, according to the natural characteristics of each space, such as a sense of openness, enclosure, tension, relief, enhancement, and hierarchical order. The spaces also reflect human scale concepts that take advantage of auditory features. The transition process after the construction of 'Namgea Suhwon' reveals the intentions of the builder to create an ecological landscape composition based on Placement and Harmony. Placement embodies' a purposeful space in which nature and the building are connected naturally, 'incomplete open space pursuing completion', and 'potential beauty in which tension and relaxation are repeated'. Harmony embodies 'order and continuity having a sense of unity with the natural environment' and the 'sharing of daily life and memory'. 'Namgea Suhwon' contains many ideas for landscape planning, land use and the design of a campus environment.

Implementation of Markerless Augmented Reality with Deformable Object Simulation (변형물체 시뮬레이션을 활용한 비 마커기반 증강현실 시스템 구현)

  • Sung, Nak-Jun;Choi, Yoo-Joo;Hong, Min
    • Journal of Internet Computing and Services
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    • v.17 no.4
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    • pp.35-42
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    • 2016
  • Recently many researches have been focused on the use of the markerless augmented reality system using face, foot, and hand of user's body to alleviate many disadvantages of the marker based augmented reality system. In addition, most existing augmented reality systems have been utilized rigid objects since they just desire to insert and to basic interaction with virtual object in the augmented reality system. In this paper, unlike restricted marker based augmented reality system with rigid objects that is based in display, we designed and implemented the markerless augmented reality system using deformable objects to apply various fields for interactive situations with a user. Generally, deformable objects can be implemented with mass-spring modeling and the finite element modeling. Mass-spring model can provide a real time simulation and finite element model can achieve more accurate simulation result in physical and mathematical view. In this paper, the proposed markerless augmented reality system utilize the mass-spring model using tetraheadron structure to provide real-time simulation result. To provide plausible simulated interaction result with deformable objects, the proposed method detects and tracks users hand with Kinect SDK and calculates the external force which is applied to the object on hand based on the position change of hand. Based on these force, 4th order Runge-Kutta Integration is applied to compute the next position of the deformable object. In addition, to prevent the generation of excessive external force by hand movement that can provide the natural behavior of deformable object, we set up the threshold value and applied this value when the hand movement is over this threshold. Each experimental test has been repeated 5 times and we analyzed the experimental result based on the computational cost of simulation. We believe that the proposed markerless augmented reality system with deformable objects can overcome the weakness of traditional marker based augmented reality system with rigid object that are not suitable to apply to other various fields including healthcare and education area.

Phytotoxicity of Endophytic Fungi and Characterization of a Phytotoxin Isolated from Gliocladium catenulatum from Pinus densiflora (식물내생곰팡이의 제초활성 검정 및 소나무에서 분리한 Gliocladium catenulatum이 생산하는 제초활성 물질의 특성 규명)

  • Choi, Gyung-Ja;Park, Joong-Hyeop;Kim, Heung-Tae;Lee, Seon-Woo;Choi, Jung-Sup;Hong, Kyung-Sik;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Mycology
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    • v.32 no.1
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    • pp.8-15
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    • 2004
  • This study was conducted to discover new phytotoxins which may be used as lead molecules for the development of new herbicides. A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 8 locations in Korea. Their herbicidal activities were screened in vivo by herbicidal and duckweed bioassays after they were cultured in potato dextrose broth and rice solid media. Both fermentation broth and solid culture extract of Gliocladium catenulatum F0006 isolated from red pine (Pinus densiflora) showed 70% herbicidal activity only against cocklebur (Xanthium strumarium) out of the 10 weeds tested. Solid culture extract of F0034 isolated from arrowroot (Pueraria thunbergiana) exhibited 20 to 100% herbicidal activities against all of the weeds. Especially, shattercane (Sorghum bicolor), barnyardgrass (Echinochloa crus-galli), large crabgrass (Digitaria sanguinalis), and fall pauicum (Panicum dichtomiflorum) were sensitive to the solid culture extract of F0034. In addition, solid culture extract of F0043 isolated from red pine displayed 20% to 70% herbicidal activities only against 5 grass species, but not against 5 broad-leaf plant species. On the other hand, as the results of duckweed assay, 8 fermentation broths showed 100% growth inhibitory activity at concentrations less than 5.0% of culture supernatants and 12 solid cultures had a potent inhibitory activity against duckweed growth. A toxic metabolite was purified from the solid cultures of G. catenulatum F0006 by repeated column chromatography and bioassay. It caused a phytotoxic syndrome only on cocklebur out of the 10 weeds tested; it completely killed cocklebur seedlings at $500\;{\mu}g/ml$ and showed 85% herbicidal activity against cocklebur at $100\;{\mu}g/ml$. The molecular weight of the toxic metabolite is 238 daltons and its structure determination is underway.

Hierarchical Overlapping Clustering to Detect Complex Concepts (중복을 허용한 계층적 클러스터링에 의한 복합 개념 탐지 방법)

  • Hong, Su-Jeong;Choi, Joong-Min
    • Journal of Intelligence and Information Systems
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    • v.17 no.1
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    • pp.111-125
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    • 2011
  • Clustering is a process of grouping similar or relevant documents into a cluster and assigning a meaningful concept to the cluster. By this process, clustering facilitates fast and correct search for the relevant documents by narrowing down the range of searching only to the collection of documents belonging to related clusters. For effective clustering, techniques are required for identifying similar documents and grouping them into a cluster, and discovering a concept that is most relevant to the cluster. One of the problems often appearing in this context is the detection of a complex concept that overlaps with several simple concepts at the same hierarchical level. Previous clustering methods were unable to identify and represent a complex concept that belongs to several different clusters at the same level in the concept hierarchy, and also could not validate the semantic hierarchical relationship between a complex concept and each of simple concepts. In order to solve these problems, this paper proposes a new clustering method that identifies and represents complex concepts efficiently. We developed the Hierarchical Overlapping Clustering (HOC) algorithm that modified the traditional Agglomerative Hierarchical Clustering algorithm to allow overlapped clusters at the same level in the concept hierarchy. The HOC algorithm represents the clustering result not by a tree but by a lattice to detect complex concepts. We developed a system that employs the HOC algorithm to carry out the goal of complex concept detection. This system operates in three phases; 1) the preprocessing of documents, 2) the clustering using the HOC algorithm, and 3) the validation of semantic hierarchical relationships among the concepts in the lattice obtained as a result of clustering. The preprocessing phase represents the documents as x-y coordinate values in a 2-dimensional space by considering the weights of terms appearing in the documents. First, it goes through some refinement process by applying stopwords removal and stemming to extract index terms. Then, each index term is assigned a TF-IDF weight value and the x-y coordinate value for each document is determined by combining the TF-IDF values of the terms in it. The clustering phase uses the HOC algorithm in which the similarity between the documents is calculated by applying the Euclidean distance method. Initially, a cluster is generated for each document by grouping those documents that are closest to it. Then, the distance between any two clusters is measured, grouping the closest clusters as a new cluster. This process is repeated until the root cluster is generated. In the validation phase, the feature selection method is applied to validate the appropriateness of the cluster concepts built by the HOC algorithm to see if they have meaningful hierarchical relationships. Feature selection is a method of extracting key features from a document by identifying and assigning weight values to important and representative terms in the document. In order to correctly select key features, a method is needed to determine how each term contributes to the class of the document. Among several methods achieving this goal, this paper adopted the $x^2$�� statistics, which measures the dependency degree of a term t to a class c, and represents the relationship between t and c by a numerical value. To demonstrate the effectiveness of the HOC algorithm, a series of performance evaluation is carried out by using a well-known Reuter-21578 news collection. The result of performance evaluation showed that the HOC algorithm greatly contributes to detecting and producing complex concepts by generating the concept hierarchy in a lattice structure.

Occurrence Characteristics and Existing Forms of U-Th Containing Minerals in KAERI Underground Research Tunnel(KURT) Granite (한국원자력연구원 지하처분연구시설(KURT) 화강암의 U-Th 함유광물 산출특성 및 존재형태)

  • Cho, Wan Hyoung;Baik, Min Hoon;Park, Tae-Jin
    • Economic and Environmental Geology
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    • v.50 no.2
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    • pp.117-128
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    • 2017
  • Occurrence characteristics and existing forms of U-Th containing minerals in KURT (KAERI Underground Research Tunnel) granite are investigated to understand long-term behavior of radionuclides in granite considered as a candidate rock for the geological disposal of high-level radioactive waste. KURT granite primarily consists of quartz, feldspar and mica. zircon, REE(Rare Earth Element)-containing monazite and bastnaesite are also identified. Besides, secondary minerals such as sericite, microcline and chlorite including quartz vein and calcite vein are observed. These minerals are presumed to be accompanied by a post-hydrothermal process. U-Th containing minerals are mainly observed at the boundaries of quartz, feldspar and mica, mostly less than $30{\mu}m$ in size. Quantitative analysis results using EPMA (Electron Probe Micro-Analyzer) show that 74.2 ~ 96.5% of the U-Th containing minerals consist of $UO_2$ (3.39 ~ 33.19 wt.%), $ThO_2$ (41.61 ~ 50.24 wt.%) and $SiO_2$ (15.43 ~ 18.60 wt.%). Chemical structure of the minerals calculated using EPMA quantitative analysis shows that the U-Th minerals are silicate minerals determined as thorite and uranothorite. The U-Th containing silicate minerals are formed by a magmatic and hydrothermal process. Therefore, KURT granite formed by a magmatic differentiation is accompanied by an alteration and replacement owing to a hydrothermal process. U-Th containing silicate minerals in KURT granite are estimated to be recrystallized by geochemical factors and parameters such as temperature, pressure and pH owing to the hydrothermal process. By repeated dissolution/precipitation during the recrystallization process, U-Th containing silicate minerals such as thorite and uranothorite are formed according to the variation in the concentrated amount of U and Th.

Improvement of Net Structure and Operating System in Purse Seine Fishery for Gizzard-shad, Konosirus Punctatns(I) -Underwater Geometry and Behaviour of fish School to the Net - (전어 선망 어구 및 조업 시스템 개발(I) -어구의 수중 형상 및 전어 어군의 대망 행동-)

  • 장덕종;신형호;김동수;김진건
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.2
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    • pp.156-163
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    • 2002
  • In order to development the construction and dimension of fishing gear for gizzard shad coastal purse seine, first of all investigated to the sinking speed and underwater geometry of net, behaviour of fish school to the net during the fishing operation In the field. The results obtained are summarized as follows; 1. Average sinking speed of net was 13cm/sec in bunt, 9.0cm/sec and 9.5cm/sec in 170m and 280m of center, 4.9cm/sec in end wing side, therefore was fastest in start of shooting and decreased gradually during the shooting process. 2. The most of leadline was reached in bottom from the shooting immediately to hauling time and the mean depth of timber bar used equipment for the escaping prevention of fish school was within the 2.7m. 3. The fish school of gizzard shad was appearanced higher density and remained to the most time in bottom than the surface and repeated to vortical movement, and its tendency to distinct in rapid time of tide current. 4. Behaviour of fish school in the net was showed to the vortical movement by sinking and rising immediately with the shooting of net and then divide with the two shape to follow round the wall of net and no patterns straightly movement in the net, and tendency easily catched in fish school of the wall of net. 5. Escaping of fish school in the gap of wing side was to busy after that seting the timber bar, therefore its function for escaping prevention of fish school was very lowed. 6. Escaping behaviour of fish school was differenced with the depth of fishing ground, the above 20m escaped to busy through the below in ledline because the sinking speed of fish school is fast than the net.