• 대한의생명과학회지
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• 제16권3호
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• pp.193-196
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• 2010

Multiplex PCR-based short tandem repeat (STR) analysis is considered as a good tool for monitoring bone marrow engraftment after sex-mismatched allogeneic transplantation and provides a sensitive and accurate assessment of the contribution of both donor and/or recipient cells in post-transplantation specimens. Forensic STR analysis and quantitative real time PCR are used to determine the proportion of donor versus recipient each contained within the total DNA. The STR markers were co-amplified in a single reaction by using commercial $PowerPlex^{(R)}$ 16 system and $AmpFISTR^{(R)}$ $Identifiler^{(R)}$ / $Yfiler^{(R)}$ PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI $PRIS^{(R)}$ 3100 Genetic Analyzer with capillary electrophoresis. The $GeneMapper^{TM}$ ID software were used for size calling and analysis of STR profiles. Extracted DNA was quantified by the $Quantifiler^{TM}$ Human DNA / Y Human Male DNA Quantification Kit The intent of this study was to analyze the ratio of donor versus recipient cells in the post-transplant peripheral blood, spleen, lung and kidney specimens. Specimens were taken from the traffic accident male victim who had been engrafted from bone marrow female donor. Blood and spleen specimens displayed female donor DNA profile. Kidney specimen showed male recipient DNA profile. Interestingly, lung tissue showed mixed profiles. The findings of this study indicate that the forensic STR analysis using fluorescence labeling PCR combined with capillary electrophoresis is quick and reliable enough to assess the ratio of donor versus recipient cells and to monitor the mixed chimeric patterns.

• 한국농공학회논문집
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• 제53권6호
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• pp.153-163
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• 2011

This research describe improved algorithm that is able to decide terminal criterion of Evolutionary Structural Optimization (ESO), reducing load of calculation to search load path of concrete beam, and apply to agricultural facilities. The ESO method is that make to discrete structure, structural analyze each element stress through FEM. And repeat generation with next material condition to become for most suitable composing. Individual element introduces concept of zero stiffness, but zero stiffness decisions are gone to direction of exclusion. In this stduy, improve algorithm to be convergence by 'Rule of Alive or Die' in arrival because is most suitable. Also, existing terminal criterion lack consistency because that used depend on experience of researcher. This research procedure is fellowed. First, all modulus of elasticity assume a half of elasticity modulus of material, Second, structural analysis by FEM, Third, apply to the remove ratio and restoration ratio for the 'rule of alive or die'. Forth, reconstruct the element and material conditions. And repeat the first to forth process. The terminal time of evolutional procedure is the all elastic modulus of element changed to blank value or elasticity modulus value of original. Therefore, in this study, consist the algorithm for programming, and apply to the agricultural facilities with concrete.

• 복식
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• 제44권
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• pp.131-141
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• 1999

The purpose of this study was to investigate the various expressions with materials in fashion illustration using CAD comparing with hand work. The design motive for this study is to be selected from Bell Epoque era which was the revolutionary period in fashion illustration. The results of this study were as follows: 1. By using CAD, water color was well expressed to repeat brushing for the clarity, darkness, and brightness. It was more effective to use CAD in layering or duplicating complex and intricate patterns because the base color gets to be concealed under the repeated oil pastel. Acryl, like oil pastel, was easily absorbed in canvas, so it is effective to repeat brushing for expressing pure color. It was inconvenient to use wax crayon for controlling the moderate opacity because wax crayon absorbed water color dye stuffs, so crayon line was concealed when repetitions were being done. 2. The advantage of using CAD was convenience for getting rid of troublesome process and inefficient works. Also, CAD had a good tool like oil pastel in the use of coloring work by using pure color. By using CAD, various expressions on materials and texture of surface can be achieved effectively. Also, it is very strong substitute for time-saving, convenience, economic aspects from providing simple instrument, and production in the state of various kinds of paper and canvas as a method of visualization. Therefore, fashion illustration using CAD provides effective way of producing works, and gives promising vision in the future.

• 한국건설관리학회논문집
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• 제3권1호
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• pp.107-114
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• 2002

최근 들어 국내 대형건설회사들을 중심으로 위험관리체계에 대한 관심이 급증하고 있으며, 각 회사들은 위험관리체계를 건설공사에 적용하기 위하여 많은 노력을 기울이고 있다. 그러나 기존의 외국 공공기관에서 적용되었던 위험관리 방법론들은 위험관리 프로세스가 일회성으로 완료되고, 프로젝트 관리자의 개인적 판단에 크게 의존하는 측면이 강하여, 이를 국내 건설공사에 적용할 경우 많은 문제점이 있는 것으로 분석되었다. 본 연구의 목적은 건설환경에 더욱 적합하고, 실용적인 위험관리 방법론으로 잔여위험을 고려한 위험대응 프로세스 모델을 제안하는 것이다. 이러한 프로세스는 특정 위험요인에 대한 위험대응 전략 수립시 전략의 효율성을 감안하여 잔여위험도를 계산한 다음 잔여위험도가 위험허용도 이하가 될 때까지 반복적으로 위험대응 전략을 수립하는 프로세스이다.

• Molecules and Cells
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• 제27권2호
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• pp.199-203
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• 2009

The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immunohistochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 Proceedings of the Korea Automatic Control Conference, 11th (KACC); Pohang, Korea; 24-26 Oct. 1996
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• pp.346-349
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• 1996

In this paper development of a CAD of control systems is introduced which enables us to do not only analysis of control systems, design of controllers but also real-time implementation of controllers. By utilizing this software, the control engineer is able to repeat the procedure of modification of controllers and experiments without recompile to attain better performance. The software also offers the facility to update the parameters of controllers without stopping real-time control, which helps on-line tuning of controllers. If some parameters of the controller is changed on-line, the control input may change discontinuously. It has serious effect on the control systems. A method for on-line tuning of state feedback controller with state observer is proposed and verified through the experiment with an inverted pendulum.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 하계종합학술대회 논문집(3)
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• pp.93-96
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• 2001

In this paper, we propose a delay-constrained retransmission method to control packet error or loss in common internet. The Delay Regulator(or Jitter Buffer) which is used to control errors caused by unreliable UDP connection, stores received data packets fDr a small amount of time to prevent network jitter from affecting display quality, which causes constant delay. In this paper, we propose a retransmission method to increase efficiency of ARQ(Automatic Repeat reQuest) by using characteristic of delay regulator.

• 한국정보전자통신기술학회논문지
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• 제6권3호
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• pp.156-161
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• 2013

가스 밸브 제어기는 사용자의 취급부주의에 의해 폭발, 화재 및 질식 등의 사고를 미연에 방지하고자 사용 시간을 설정하여 가스를 사용할 수 있는 시스템이다. 가스 밸브는 각 수용가에서 한 번 설치를 하면 특별한 사안이 없는 한 교체하지 않는 품목 중의 하나로서, 설치 이전에 충분한 시험이 필요한 장치이다. 본 연구에서는 가스 밸브 제어기를 사용 횟수를 설정하여 시험할 수 있는 시스템을 개발하였다.

• 분석과학
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• 제8권4호
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• pp.715-722
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• 1995

The structural characterization for series of polyesters has been done by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Polymer fragments and intact oligomers composed of large numbers of repeat units have been investigated. Transesterification of polyesters in trifluoroacetic acid (TFA) and chlorodifluoroacetic acid (CFA) was monitored and reaction products were identified using TOF-SIMS. The shapes and intensities of clusters in transesterification spectra show good agreement with the theoretical isotope pattern. TOF-SIMS spectra were used to obtain information about the progress of the transesterification reaction.

• 대한원격탐사학회지
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• 제28권1호
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• pp.121-128
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• 2012

조간대 지역의 고해상 지형도는 해수 운동에 의한 퇴적 및 침식량의 변화와 같이 과학적인 연구의 기초자료로 쓰일 뿐만 아니라 연안 습지의 실제적인 관리 및 환경변화 모니터링의 도구로서도 그 활용의 가치가 높다. 그러나 조간대는 하상 퇴적물의 유입과 조석현상에 의해 빠르게 변화하고 있어 원격탐사와 같이 단기간에 넓은 지역을 조사할 수 있는 기술의 적용이 필요하다. 레이더 간섭기술은 가장 보편적이면서 성공적인 고해상 지형도 작성 기술로 알려져 있으나, 조간대에서는 지표노출시간의 제약 및 빠른 상태변화로 인해 일반적인 repeat-pass 간섭기술로는 충분한 긴밀도를 얻기 힘들다. 이 연구에서는 재방문주기가 짧은 Cosmo-SkyMed 1일 간격 관측자료와 ERS2-ENVISAT 30분 간격 관측자료를 이용하여 레이더 간섭기술을 이용한 조간대 지형도 작성을 실험하였다. 두 자료 모두 긴 수직기선으로 인한 높은 고도민감도와 짧은 관측주기로 인한 높은 긴밀도로 간섭위상을 구할 수 있었으나, Cosmo-SkyMed 관측자료에서는 대기에 의한 위상지연 효과와 조석에 의한 조간대 표층의 함수율 변화로 인하여 조간대와 같이 평평한 지역의 지형정보를 추출하는데에 어려움이 발생한다. 반면에 ERS2-ENVISAT 관측자료는 이종센서간의 관측중심주파수 차이를 상쇄하기 위해 설정된 긴 수직기선 덕분에 매우 높은 고도민감도를 가지며, 30분이라는 짧은 관측주기는 간섭쌍을 이루는 두 영상에 포함된 대기지연효과를 대부분 상쇄시키므로 조간대의 지형도 작성에 이상적인 자료로 판단된다.