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Production of Nitric Oxide in Raw 264.7 Macrophages treated with Ganoderan, the ${\beta}-Glucan$ of Ganoderma lucidum (영지의 균사체성 ${\beta}-glucan$에 의한 Raw 264.7 대식세포의 Nitric Oxide생성)

  • Han, Man-Deuk;Lee, Eun-Sook;Kim, Young-Kweon;Lee, June-Woo;Jeong, Hoon;Yoon, Kyung-Ha
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.246-255
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    • 1998
  • Ganoderan (GAN), an immunomodulating ${\beta}-glucan$ of G. lucidum, induces potent antitumor immunity in tumor-bearing mice. This study was set up to elucidate the ability of macrophage activation of GANs. GAN-treated Raw 264.7 macrophages showed enhanced production of nitric oxide (NO). The ability of GANs to produce NO was based on differences in chemical composition of GANs obtained from the mycelium on various carbon sources and mycelial fractionation. The highest NO production was observed in CW-AS-WS polysaccharide which was extracted from the mycelial wall. GAN-treated Raw 264.7 cells gave a 2-to 5-fold (24 hr) formation of NO levels compared with those treated with medium only. Partial removal of the protein in the extracellular GAN by TCA treatment did appreciably reduce its capacity to secrete NO. The mixture effect of GAN and LPS increased the nitric oxide secretion from RAW 264.7. The cell proliferation of GAN-treated Raw 264.7 cell tines inhibited as compared with its control. Of the culture supernatant of macrophage activated by GAN, the percentage of cytotoxicity against mouse leukemia L1210 cells was slightly dependent on the amount of NO in the culture supernatants of the activated-macrophages. These results indicate that the ${\beta}-glucan-related$ polysaccharides of the higher fungus activate macrophage and release nitric oxide. It also suggests that murine macrophages possess certain receptors for ${\beta}-anomeric$ glucans and play a critical role of ${\beta}-glucan-related$ tumor killing mechanism.

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Improvement of Seedling Stand and Lodging Prevention in Direct Seeded Rice (벼 직파재배(直播栽培) 입묘율향상(立苗率向上)과 도복경감(倒伏輕減))

  • Oh, Yun-Jin;Kim, Chung-Kon
    • Korean Journal of Weed Science
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    • v.12 no.3
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    • pp.200-222
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    • 1992
  • The results of recent researches for improvement of seedling stand in direct seeded rice on the dry paddy in Korea were summarized as the following ; a variety to be cultivated should be chosen the characteristics of high percentage germination under low temperature, shorter period of shoot emergence, and better growth of the mesocotyl and shoots. Meanwhile, there was 40% increase in seedling stand at the treatment of removal of the seed awn under using the drill seeder. After seeding the rice seed covered with soil of 3cm depth was better seedling emergence and also there was the hightest seedling emergence at the 70% of moisture content of the soil. In addition, the application of the Release containing GA 10% enabled to increase the seedling stand and furthermore it was effective under deep seeding depth. The optimum seeding date should be seeded around May 10 when mean air temperature is above 12-13$^{\circ}C$ so that may establish more less 70% in seedling stand. Based on an appropriate seedling stand of 150/$m^2$, the optimum seeding rate was 5kg/10a. It was the best in seeding method using drill seeder and the most desirable recommended seeding method was the drill seeder in terms of seedling stand. In order to improve seedling stand water management was more effective in canal irrigation and in drainage at 6hr after irrigation following by the seeding process. On the other hand, for the increase of seedling stand under flooded condition a variety might have characters being better germination at low concentration of dissolved oxygen and vertically deeper growing of the crown root. Also, seedling stand was able to increase with the seed coating of $CaO_2$in the flooded soil. It was possible to be seeded on the early part of May being mean air temperature of avove 10$^{\circ}C$ and the optimum seeding rate was 5kg/10a. For an effective water management water would be flooded up to 3cm depth for 2-3 weeks after seeding. The rice plant grown under the direct seeded cultivation might be not so much strong in lodging resistance compared to that grown under the transplanting and moreover direct seeded rice cultivation under flooded condition would be more weak growth of the rice plant than that on dry paddy. Meanwhile, the lodging would be affected by the seeding rate, the soil depth after seeding. and seeding method even in the same variety. In particular, roots in the lodging pattern of direct seeded rice cultivation under flooded condition were largely distributed on the soil surface so that resulted easily in the lodging. In general, the lodging resistance would be greater as seeding rate and amount of N fertilizer application are lower and soil depth after seeding is higher. Among the introduction of different seeding method the high ridged drill seeding method on dry paddy soil resulted in the lowest in the lodging index and also it was lower in the drill seeding method than in the scattering seeding method under flooded condition. In case of more than 150 seedlings per $m^2$ there was a severe lodging due to high lodging index at the 3rd and 4th internodes. The effective lodging prevention was able to at the treatment of the Inabenfide at 45 days before heading and the Uniconazol at 15 days before heading which caused the shortage by 10-15cm in culm length. Also, fertilizer management using split application of nitrogen would be contributed the reduction of lodging at the rate of 20-30-20-20-10%(basal-5th leaf stage-7th leaf stage-panicle initiation stage-heading stage) on the dry paddy soil.

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Skeletal relapse and dental change during intermaxillary fixation after mandibular setback (외과적 하악 후퇴술 후 악간고정기간 중의 골격성 재발과 치열의 변화)

  • Chang, Chong-On
    • The korean journal of orthodontics
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    • v.29 no.4 s.75
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    • pp.457-466
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    • 1999
  • It has been reported that skeletal relapse and dental change after mandibular setback do occur not only after intermaxillary fixation(IMF) removal but also during IMF The side effects of skeletal relapse during IMF have clinical importance because they can cause many Postoperative orthodontic Problems. Generally, the Prevention of solid union between segments, compensatory tooth movement, anterior openbite, etc. have been cited as the side effects of jaw displacement. The purpose of this study was to evaluate the skeletal relapse and dental change during IMF. The material consisted of 28 patients who were treated by BSSRO(bilateral sagittal split ramus osteotomy), wire osteosynthesis, IMF for correction of mandibular prognathism. Through cephalometric analysis, the amount and direction of surgical movement, skeletal relapse and dental change during IMF were measured. The correlation between surgical movement and skeletal relapse, between skeletal relapse and dental changes were evaluated. The following conclusions were obtained; 1. Distal segment was repositioned backward and upward, proximal segment showed clockwise rotation during surgery. 2. During ]m, anterior portion of distal segment was displaced backward and posterior portion was displaced upward. Proximal segment was displaced upward with forward movement of p-Go(gonion of proximal segment). Backward surgical movement of p-GO was significantly correlated with forward displacement of p-Go. 3. Overjet and overbite were not changed during IMF. The compensatory tooth movements during IMF were characterized by retroclination of upper incisors md retroclination, extrusion of lower incisors. These compensatory tooth movements had statistically significant correlation with upward displacement of d-Go (gonion of distal segment).

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Influence of bearing surface angle of abutment screw on mechanical stability of joint in the conical seal design implant system (내부 원추형 연결형태 임플란트에서 지대주 나사머리의 좌면각도가 연결부 기계적 안정성에 미치는 영향)

  • Kim, Joo-Hyeun;Huh, Jung-Bo;Yun, Mi-Jung;Kang, Eun-Sook;Heo, Jae-Chan;Jeong, Chang-Mo
    • Journal of Dental Rehabilitation and Applied Science
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    • v.30 no.3
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    • pp.206-214
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    • 2014
  • This study is to evaluate how different bearing surface angles of abutment screw affect the mechanical stability of the joint in the conical seal design implant system. Materials and Methods: Internal connection type regular implants, two-piece cemented type abutments and tungsten carbide/carbon-coated titanium alloy abutment screws were selected. Titanium alloy screws with conical ($45^{\circ}$) and flat ($90^{\circ}$) head designs which fit on to abutment were fabricated. The abutments were tightened to implants with 30 Ncm by digital torque gauge. The loading was applied once to the central axis of abutment. The mean axial displacement was measured using micrometer before and after the tightening and loading (n = 5). The abutment was tightened to implants with 30 Ncm and T-shape stainless steel crown was cemented. Then the change in the amount of reverse-torque was measured after the repeated loading to the central axis, and the place 5 mm away from the central axis. Compressive bending and fatigue strength were measured at the place 5 mm away from the central axis (n = 5). Results: Both groups showed the largest axial displacement when abutment screw tightening and total displacement was greater in the flat head group compared to conical head group (P < 0.05). However, there were no significant differences in reverse torque value, compressive bending and fatigue strength (P > 0.05). Conclusion: Within the limitations of this study, the abutment screw head design had no effect on two groups regarding the joint stability, however the conical head design affected the settlement of abutment resulting in the reduced total displacement.

Comparisons in Volumes of Irrigation and Drainage, Plant Growth and Fruit Yield under FDR Sensor-, Integrated Solar Radiation-, and Timer-Automated Irrigation Systems for Production of Tomato in a Coir Substrate Hydroponic System (토마토 코이어 수경재배에서 FDR센서, 적산일사량센서 및 타이머 급액방식에 따른 급배액량, 생육 및 과실수량 비교)

  • Choi, Eun-Young;Kim, Hee-Yong;Choi, Ki-Young;Lee, Yong-Beom
    • Journal of Bio-Environment Control
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    • v.25 no.1
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    • pp.63-70
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    • 2016
  • Water drainage from the open hydroponics often causes significant environmental pollution due to agrochemicals and loss of water and nutrients. The objectives of this study were to show the potential application of an irrigation schedule based on threshold values of volumetric substrate water content for tomato (Solanum lycopersicum L. 'Samsamgu') cultivation in a commercial hydroponic farm during spring to summer cultivation. This study was performed for minimizing effluent from coir substrate hydroponics using a frequency domain reflectometry (FDR) sensor-automated irrigation, as compared with an integrated solar-radiation (IR) and conventional timer-irrigation (TIMER) after transplanting. In results, no significant difference in daily irrigation volume was found among the treatments until 88 days after transplant (DAT). However, during the 88 to 107 DAT, the daily irrigation volume was in the order of IR (2125 mL) > TIMER (2063 mL) > FDR (1983 mL), and during the 108 to 120 DAT, it was in the order of IR (2000 mL) > TIMER (1664 mL) > FDR (1500 mL). The lowest drainage volume was observed in the FDR treatment with the order of IR (12~19%) > TIMER (4~12%) > FDR (0~7%) during the entire growing period. A lower irrigation volume in the FDR treatment after 88 DAT may be due to the sensor's detecting capacity for less water absorption by plant after completing fruit maturity with apical pruning and removal of lower leaves, while a higher irrigation volume in the IR treatment may be due to gradual increase in integrated solar-radiation amount as closer to summer season. There was no significant difference in plant growth and fruit yield among the treatments; however, a 11% and 18% of higher soluble sugar content was observed in the FDR than that of TIMER and IR treatment. respectively.

Electrochemical Treatment of Dye Wastewater Using Fe, RuO2/Ti, PtO2/Ti, IrO2/Ti and Graphite Electrodes (RuO2/Ti, PtO2/Ti, IrO2/Ti 및 흑연전극을 이용한 염료폐수의 전기화학적 처리)

  • Kim, A Ram;Park, Hyun Jung;Won, Yong Sun;Lee, Tae Yoon;Lee, Jae Keun;Lim, Jun Heok
    • Clean Technology
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    • v.22 no.1
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    • pp.16-28
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    • 2016
  • Textile industry is considered as one of the most polluting sectors in terms of effluent composition and volume of discharge. It is well known that the effluents from textile dying industry contain not only chromatic substances but also large amounts of organic compounds and insolubles. The azo dyes generate huge amount of pollutions among many types of pigments. In general, the electrochemical treatments, separating colors and organic materials by oxidation and reduction on electrode surfaces, are regarded as simpler and faster processes for removal of pollutants compared to other wastewater treatments. In this paper the electrochemical degradation characteristics of dye wastewater containing CI Direct Blue 15 were analyzed. The experiments were performed with various anode materials, such as RuO2/Ti, PtO2/Ti, IrO2/Ti and graphite, with stainless steel for cathode. The optimal anode material was located by changing operating conditions like electrolyte concentration, current density, reaction temperature and initial pH. The degradation efficiency of dye wastewater increased in proportion to the electrolyte concentration and the current density for all anode materials, while the temperature effect was dependent on the kind. The performance orders of anode materials were RuO2/Ti > PtO2/Ti > IrO2/Ti > graphite in acid condition and RuO2/Ti > IrO2/Ti > PtO2/Ti > graphite in neutral and basic conditions. As a result, RuO2/Ti demonstrated the best performance as an anode material for the electrochemical treatment of dye wastewater.

Studies on the Mineralogical Characteristics of Apple Orchard Soils (사과원토양(園土壤)의 광물학적특성(鑛物學的特性)에 관(關)한 연구(硏究))

  • Lee, Mahn Jung
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.3
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    • pp.141-152
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    • 1973
  • The mineralogical studies of the eleven sub-soil samples derived from granite, granodiorite, diorite and arkose sandstone, taken from apple orchards in the province of Kyungsangbukdo, Korea are made to investigate the relationships between the mineral weathering, soil forming processes and mineralogical composition. The fine sand fraction (less than 0.2mm) and the clay fraction (less than 2 micron) are dispersed with the shaker after hydrogen peroxide treatment for the removal of organic matter, and separated from each suspension by gravity sedimentation. The fine sand are observed by mineral microscope and the clay are observed by X-ray diffraction patterns, differential thermal analysis curves and infrared spectrum. The outline of the results are as follows. 1. The primary minerals ; Quartz, changed-feldspar, plagioclase, alkali-feldspar are dominant in almost all samples, and some samples contain an appreciable amount of hornblende, biotite, muscovite and plant opal. There are also those samples which contain very small quantity of pyroxene group, tourmaline, epidote, cyanite, magnetite, volcanic glass and zircon. They are mainly derived from weathering products of granite, granodiorite, diorite, arkose or its mixtures. 2. All samples contain expanding or nonexpanding $14{\AA}$ minerals, illite and kaolin minerals, and some samples contain chlorite, cristobalite, gibbsite, and those primary minerals as quartz and feldspar, but the quantities vary according to the parent matrials. 3. Non-expanding $14{\AA}$ minerals may be dioctahadral vermiculite which sandwiches gibbsite layer or chlorite in between layer lattices. 4. As for clay minerals, montmorillonite was principal component in the samples derived from weathering products of arkose sandstone and tertiary. Minerals which are derived from weathering products of arkose have kaolin minerals and vermiculite as their principal component, and minerals derived from weathering products of acidic rock group are generally classified into two groups, the kaolin mineral group, and the kaolin minerals and vermiculite group.

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Salt Accumulation in Horticultural Soils of PE Film House in Chungbuk Area (충북지역(忠北地域) 시설원예재배지(施設園藝栽培地) 토양(土壤)의 염류집적(鹽類集積) 실태조사(實態調査))

  • Yuk, Chang-Soo;Kim, Jai-Joung;Hong, Soon-Dal;Kang, Bo-Goo
    • Korean Journal of Soil Science and Fertilizer
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    • v.26 no.3
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    • pp.172-180
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    • 1993
  • Chemical properties of the soils were surveyed in the field of vinyl houses concentrated in the area of Bunpyong-dong and Shinchon-dong of Cheongju, Chilgeum-dong of Chungju and Gageum-myeon of Jungweon-gun. Chungcheongbuk-do province. 1. Content of chemical component of the soil in the vinyl house was higher than in open field. In more than half of the vinyl house soils surveyed, electric conductivity was more than 4.0 mmhos/cm and available phosphate was more than 1,000ppm. 2. Contents of availble phosphate and exchangeable potassium were increased with years of cultivation and their content of accumulation in soil were in the order of Bunpyong-dong>Shinchon-dong>Gageum-myeon>Chilgeum-dong. While their mobility was comparatively low. 3. $NO_3$-N content was remarkably higher in vinyl house soil and the older in cultivation made $NO_3$-N content higher same as the case of available phosphate content. However easy leaching of $NO_3$-N through soil profile is expected due to the fact that $NO_3$-N content was rapidly decreased by removal of polyethylene film cover from the frame of house after harvest of crops. 4. It is a tendency that various chemical contents of vinyl house soils wer higher in 1992 than in 1988~1990, especially much higher from the area of Bunpyong-dong and Shinchon-dong of Cheongju. 5. Salt accumulation in vinyl house soil has been increased with continuing cultivation. Therefore amount of fertilizer application should be controlled in order to avoid salt toxicity, quality deterioration for crop and salt contamination of ground water.

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Clinical Experiences of Cardiac Surgery Using Minimal Incision (소절개선을 이용한 심장수술의 임상고찰)

  • Kim, Kwang-Ho;Kim, Joung-Taek;Lee, Seo-Won;Kim, Hae-Sook;Lim, Hyun-Kung;Lee, Choon-Soo;Sun, Kyung
    • Journal of Chest Surgery
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    • v.32 no.4
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    • pp.373-378
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    • 1999
  • Background: Minimally invasive technique for various cardiac surgeries has become widely accepted since it has been proven to have distinct advantages for the patients. We describe here the results of our experiences of minimal incision in cardiac surgery. Material and Method: From February 1997 to November 1998, we successfully performed 31 cases of minimally invasive cardiac surgery. Male and female ratio was 17:14, and the patients age ranged from 1 to 75 years. A left parasternal incision was used in 9 patients with single vessel coronary heart disease. A direct coronary bypass grafting was done under the condition of the beating heart without cardiopulmonary bypass support(MIDCAB). Among these, one was a case of a reoperation 1 week after the first operation due to a kinked mammary artery graft. A right parasternal incision was used in one case of a redo mitral valve replacement. Mini-sternotomy was used in the remaining 21 patients. The procedures were mitral valve replacement and tricuspid annuloplasty in 6 patients, mitral valve replacement 5, double valve replacement 2, aortic valve replacement 1, removal of left atrial myxoma 1, closure of atrial septal defect 2, repair of ventricular septal defect 2, and primary closure of r ght ventricular stab wound 1. The initial 5 cases underwent a T-shaped mini-sternotomy, however, we adopted an arrow-shaped ministernotomy in the remaining cases because it provided better exposure of the aortic root and stability of the sternum after a sternal wiring. Result: The operation time, the cardiopulmonary bypass time, the aorta cross-clamping time, the mechanical ventilation time, the amount of chest tube drainage until POD#1, the chest tube indwelling time, and the duration of intensive care unit staying were in an acceptable range. There were two surgical mortalities. One was due to a rupture of the aorta cannulation site after double valve replacement on POD#1 in the mini-sternotomy case, and the other was due to a sudden ventricular arrhythmia after MIDCAB on POD#2 in the parasternal incision case. Postoperative complications were observed in 2 cases in which a cerebral embolism developed on POD#2 after a mini-sternotomy in mitral valve replacement and wound hematoma developed after a right parasternal incision in a single coronary bypass grafting. Neither mortality nor complication was directly related to the incision technique itself. Conclusion: Minimally invasive surgery using parasternal or mini-sternotomy incision can be used in cardiac surgeries since it is as safe as the standard full sternotomy incisions.

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Estimation of Timber Production by Thinning Scenarios Using a Forest Stand Yield Model (임분 수확예측 모델을 이용한 간벌 시나리오별 목재수확량 예측)

  • Kim, Young-Hwan;Kim, Tae-Wook;Won, Hyun-Kyu;Lee, Kyeong-Hak;Shin, Man Yong
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.592-598
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    • 2012
  • Forest stand yield and its changes along with 10 thinning scenarios were estimated using a forest stand yield model for six major tree species in Korea, such as Pinus densiflora in Gangwon province, Pinus densiflora in other regions, Pinus koraiensis, Larix leptolepis, Quercus acutissima Carruth, Quercus mongolica. The 10 thinning scenarios were generated based on a number of constraints and assumptions. For instance, it was assumed that thinning is implemented between 15 years and 40 years with 5 year period and its duration should be at least 10 years. Also, the overall removal rate from the thinning treatments was assumed to be not greater than 60%. Under the 10 scenarios, the overall stand yield volumes from thinning and final harvesting were estimated for each species and site index. The results showed that highest yield volumes were obtained for Pinus densiflora in Gangwon province, Pinus koraiensis and Quercus mongolica when 30% of basal areas were thinned at 20 and 40 years, while highest yield volumes were obtained for Pinus densiflora in other regions and Larix leptolepis when 20% of basal areas were thinned at 20, 30 and 40 years. Those two scenarios gave the same amount of highest yield volume for Quercus acutissima Carruth. Also the results indicated that thinning treatment is effective to increase overall stand yield volume and its effects are larger with a higher site index. The largest thinning effects were found in Pinus densiflora in Gangwon province (28%) and Larix leptolepis (25%), while limited in Pinus koraiensis (12%). The forest stand yield model, used in this research, could be an effective tool for estimating the stand dynamics with various thinning treatments, but it could be improved in a further research that validates its applicability in the field.