• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.179-188
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• 2017

White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the $7^{th}$ day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.

• The Korean Journal of Pain
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• 제19권1호
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• pp.81-86
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• 2006

Background: Complex regional pain syndrome (CRPS) is a painful and disabling disease, yet the diagnosis of this can be difficult to confirm by purely objective measures. Therefore, we performed three-phasic bone scans and thermography as a work up in order to determine their predictive value and usefulness for making the diagnosis of CRPS. Methods: 44 patients who had been diagnosed with CRPS type-1, according to the modified criteria, were evaluated. All the patients were examined by performing a three-phasic bone scan and thermography as part of a work-up for diagnostic confirmation. The diffuse increased tracer uptake in the delayed image (phase III) was estimated by the positive findings. The findings were considered positive for CRPS if the thermographic findings showed temperature asymmetries between the affected and non- affected extremities of more than $1.00^{\circ}C$ Results: A review of the three-phasic bone scan for 44 patients indicated that 16 patients (36.4%) had diffusely positive scans, and thermographic abnormalities were noted in 35 of 44 patients (79.5%). Conclusions: The use of thermography in clinical settings can play an important role in the diagnosis of CRPS. However, a three-phasic bone scan alone cannot provide a completely accurate diagnosis, so it is imperative that the three-phasic bone scan data be integrated with the clinical evaluation and the other relevant tests.

• 한국터널지하공간학회 논문집
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• 제4권4호
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• pp.277-286
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• 2002

터널을 설계함에 있어서 굴착방법이나 지보패턴을 결정할 때 어려움을 겪는 주된 요인은 현지 지반에 작용하는 응력조건 및 암반상태를 정확히 파악하는데 한계가 있기 때문이다. 현장 장비의 제약, 터널을 굴착 위치까지 접근성이 난이함 등의 기술적인 제약뿐만 아니라 최근에는 민원이나 각종 인허가 등으로 더욱 많은 제약요건이 존재한다. 그럼에도 불구하고 최근들어 대안설계나 턴키설계를 통하여 직접적인 시추에 의존하지 않더라도 미지의 산악터널구간에 대한 지반정보를 획득할 수 있는 고급화된 물리탐사기술이 눈부시게 발전하는 추세이며 이를 통하여 터널굴착구간의 암반에 대한 직 간접적인 지반정보를 입수할 수 있다. 인공신경망 (ANN)의 장점은 이러한 적은 양의 지반정보와 생물학적인 로직화 과정을 통하여 입력변수에 대한 보다 신뢰성있는 결과를 제공하여 준다는 것이다. 본 연구에서는 미지의 터널굴착구간에 대한 예비 지반정보를 입력항목으로 하여 인공신경망의 오류역전파 학습알고리즘기법에 의하여 학습된 패턴을 가지고 미지의 터널굴착구간에 대한 예비 암반분류 (RMR)를 수행하는데 그 목적을 두었다. 이를 위하여 연장 4km에 달하는 ${\triangle}{\triangle}$터널현장에 대한 인공신경망 모형적용시 입력자료에 대한 적정성을 사전 평가하였고, 그 이후에 물리탐사자료를 입력변수로 활용하여 미지의 터널구간에 대한 RMR을 예측하였다. 그 결과 자료의 일치성이나 예측 RMR에 대한 신뢰도가 높은 것으로 나타났으며, 향후에는 학습효과를 높이기 위한 입력변수의 민감도 분석 (sensitivity analysis)수행 및 모델과정에서 노출된 몇가지 문제점 보완등을 통하여 설계에 적극적으로 활용하고자 한다.

• 정보관리학회지
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• 제32권1호
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• pp.23-41
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• 2015

이 연구는 국제학술지에 나타난 '동해' 관련 연구에서 '동해' 표기유형별 특성을 계량적으로 분석한 것이다. 이 때 동해표기 유형은 '동해 단독', '일본해 단독', '병행표기'로 구분하였다. 분석을 위해 Web of Science DB에서 총 4,192편을 대상으로, 표기유형별 시계열 변화, 저자 소속국가별 표기유형, 연구주제의 차이, 피인용도, 연구협력 및 공저 네트워크를 분석하였다. 그 결과 '동해' 연구에서 '일본해 단독 표기'의 비율이 가장 많은 것으로 나타났고, 1990년대 이후 '동해 단독 표기'과 '병기'의 비율이 지속적으로 상승하고 있었다. 또한 '동해' 연구의 핵심국가는 '일본', '러시아', '한국', '미국', '중국' 5개국이며, '일본해 단독 표기'의 경우 '일본'을 중심으로 '미국', '러시아', '중국'과 공동연구가 활발히 이루어지며, '동해 단독 표기'와 '병기'는 '한국' 연구자를 중심으로 '미국', '일본'과의 연구의 비율이 높았다. 공저 네트워크는 '일본해 단독 표기'의 경우 하나의 "거대 구성집단"(Giant Component)을 형성하여 이(異) 집단 간의 협업이 활발히 이루어지고 있으나, '동해 단독 표기'의 경우 소속기관을 중심으로 소규모의 연구그룹이 분산된 것으로 나타났다.

• Journal of Biosystems Engineering
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• 제43권1호
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• pp.72-80
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• 2018

Purpose: A modern greenhouse consists of various Information and Communications Technology (ICT) components e.g., sensor nodes, actuator nodes, gateways, controllers, and operating softwarethat communicate with each other. The interoperability between these components is an essential characteristic for any greenhouse control system. A greenhouse control system could not work unless the components communicate via common interfaces. The TTAK.KO-06.0288 is an interface standard consisting of four parts. Notably, TTAK.KO-06.0288-Part3, which describes the interface between a greenhouse operating system (GOS) and a greenhouse control gateway (GCG), is the core standard of TTAK.KO-06.0288. The objectives of this study were to analyze the TTAK.KO-06.0288-Part3 standard, to suggest alternative solutions for identified issues, and to develop a library as a proof of the alternative solutions. Methods: The "data field" was analyzed using a comparative analysis method, since it is a data transmission unit of TTAK.KO-06.0288-Part3. It was compared with other parts of TTAK.KO-06.0288 in terms of definition, format, size, and possible values. Although TTAK.KO-06.0288-Part1 and TTAK.KO-06.0288-Part2 do not use a "data field," they have a similar data structure. That structure was compared with the "data field" of TTAK.KO-06.0288-Part3. Results: Twenty-one issues were identified across four categories: inter-standard issues, intra-standard issues, operational issues, and misprint issues. Since some of the issues can raise interoperability problems, 16 alternative solutions were suggested. In order to prove the alternative solutions, an open-source communication library called libtp3 was developed. The library passed 14 unit tests and was adapted to two research. Conclusions: Although TTAK.KO-06.0288-Part3 is an interface standard for communication between a GOS and a GCG, it might not communicate between different implementations because of the identified issues in the standard. These issues could be solved by the alternative solutions, which could be used to revise TTAK.KO-06.0288. In addition, a relevant organization should develop a program for compatibility testing and should pursue test products for smart greenhouses.

• Asian Pacific Journal of Cancer Prevention
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• 제15권12호
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• pp.4787-4793
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• 2014

Background: Many cancer patients experience poor pain control due to various factors, including misconceptions regarding the use of opioid analgesics. For management of cancer pain, interventions involving education of both patients and physicians have been attempted. Objectives: This review aimed to assess the current evidence of the benefits of education for the management of cancer pain. Methods: We searched the Medline, EMBASE, Cochrane library, and major Korean databases to identify relevant studies. We included most study designs, but excluded case series. The primary outcomes were pain intensity and quality of life (QoL). Two reviewers assessed the risk of bias using the Cochrane's tool for RCT and Risk of Bias Assessment tool for Non-randomized Studies (RoBANS) for non-randomized studies, independently. Results: After extensive searches, 3,324 publications were screened, and 32 studies were selected. The education interventions used in the included studies included a wide variety of education methods, but the most common method was a booklet produced for patients. Regardless of the education method used, the results of the meta-analysis were as follows. The SMDs of the most severe, average, and current pain in the RCTs were significant. The SMD of worst, average, and current pain were -0.34 (-0.55, -0.13), -0.40 (-0.64, -0.15), and -0.79 (-1.35, -0.23). In the non-randomized studies, the effects on average pain were significant, but those on worst and current pain were not. Conclusions: Education intervention reduced the pain of cancer patients. Therefore, patient education could be considered to be an effective method of cancer pain management. However, our data should be interpreted with caution, and studies using standardized protocols are needed to confirm these observations.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9731-9737
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• 2014

Large scale secular registry or surveillance systems have been accumulating vast data that allow mathematical modeling of cancer incidence and mortality rates. Most contemporary models in this regard use time series and APC (age-period-cohort) methods and focus primarily on predicting or analyzing cancer epidemiology with little attention being paid to implications for designing cancer registry, surveillance or evaluation initiatives. This research models age-specific cancer incidence rates using logistic growth equations and explores their performance under different scenarios of data completeness in the hope of deriving clues for reshaping relevant data collection. The study used China Cancer Registry Report 2012 as the data source. It employed 3-parameter logistic growth equations and modeled the age-specific incidence rates of all and the top 10 cancers presented in the registry report. The study performed 3 types of modeling, namely full age-span by fitting, multiple 5-year-segment fitting and single-segment fitting. Measurement of model performance adopted adjusted goodness of fit that combines sum of squred residuals and relative errors. Both model simulation and performance evalation utilized self-developed algorithms programed using C# languade and MS Visual Studio 2008. For models built upon full age-span data, predicted age-specific cancer incidence rates fitted very well with observed values for most (except cervical and breast) cancers with estimated goodness of fit (Rs) being over 0.96. When a given cancer is concerned, the R valuae of the logistic growth model derived using observed data from urban residents was greater than or at least equal to that of the same model built on data from rural people. For models based on multiple-5-year-segment data, the Rs remained fairly high (over 0.89) until 3-fourths of the data segments were excluded. For models using a fixed length single-segment of observed data, the older the age covered by the corresponding data segment, the higher the resulting Rs. Logistic growth models describe age-specific incidence rates perfectly for most cancers and may be used to inform data collection for purposes of monitoring and analyzing cancer epidemic. Helped by appropriate logistic growth equations, the work vomume of contemporary data collection, e.g., cancer registry and surveilance systems, may be reduced substantially.

• 무역상무연구
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• 제36권
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• pp.87-109
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• 2007

This Comment explores the ICSID case of CMS Gas Transmission Company v. Argentine Republic, awarded on May 12, 2005. The Part II of this Comment first describes the relevant facts of the case including the some background for readers' understanding and the Part III summaries the claimant's requests and the decisions rendered by the Arbitral Tribunal in the Award. At Part IV, the Comment addresses the issue of determinating laws applicable to the merits of dispute in case that the parties of the case have not chosen a governing law, and at Part V, takes a close look into three main issues of (i) the indirect expropriation of the investment, (ii) the breach of fair and equitable treatment and (iii) the protections under umbrella clauses. In this CMS case, we see first that while the Tribunal affirmed that any indirect expropriation can occur from incidental interference depriving the foreign investor of the use or reasonable-to-be-expected economic benefit even if not necessarily to the obvious benefit of the host State, the Tribunal denied the occurrence of indirect expropriation in this case by holding that the Government of Argentina has not breached the standard of protection laid down in the Treaty. Secondly, however, regarding the issue of fair and equitable treatment, we see that the Tribunal, finding Argentina's breach of obligations, affirmed that the foreign investor can expect the host State to act in a consistent manner, free from ambiguity and totally transparently in its relations with the foreign investor, which can give the foreign investor certain degree of foreseeability. Thirdly and finally, we see that, on base of the effect of the umbrella clause, the Tribunal recognized the obligation of the host State undertaken not to freeze the tariff regime or subject it to price controls and not to alter the basic rules governing contracts between the foreign investor and the host State without the first's written consent. However, the protection under the umbrella clause is available only when there is a specific breach of rights and obligations under BIT or a violation of contract rights protected under BIT.

• 한국콘텐츠학회논문지
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• 제18권7호
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• pp.28-37
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• 2018

본 논문에서는 실내 인테리어에 적용 가능한 조명 디자인을 기본으로 생각하고 연구하였으며 나아가 근린공원에 설치될 공익시설물을 대상으로 공공디자인의 목적도 함께 연구하여 합리적 사용을 추구하는 모듈형 가변식 실내조명 제품디자인을 연구하고자 한다. 이러한 연구를 위하여 모듈의 기본적인 개념을 알 수 있는 선행연구 고찰과 실내디자인에 적용 가능한 조명과 실내에 대한 선행사례를 분석하였다. 이러한 분석을 토대로 본 연구에서는 모듈개념을 적용한 조명디자인의 새로운 컨셉을 도출을 위해 제품실무 개발을 위한 디자인 방향과 프로세스, 제품보관, 조립, 관리가 편리한 모듈 조립형 디자인을 제시하여 향후 조명디자인 개발에 관련된 합리적 유지관리 방향을 살펴보고 새로운 제품디자인개발에 반영할 수 있는 디자인 개발사례를 제시하였다. 또한 인지전략 프로세스를 통해 디자인을 진행하였으며 이러한 프로세스는 개념적 전략으로 실내환경 분석단계, 조명유지 보수단계, 가변형 메커니즘단계, 감성활성화 단계를 적용하여 연구를 진행하였다. 이러한 단계적특성은 디자인적인 조형적 아름다운과 접목시켜 MLD(Module Lamp Design) 전략을 제시하여 인간이 느낄 수 있는 공간에 대한 자유로운 적용을 모듈제품과 접목시키고 인간의 시각적접근과 바라보며 자유롭게 생각하는 개념적 가치를 담아 MLD인지전략 디자인 프로세스에 적용시켜 개발 하고자 하였다.

• 한국산학기술학회논문지
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• 제21권8호
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• pp.370-378
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• 2020

COBie(Construction Operations Building Information Exchange)는 시설물 관리 프로젝트에 필요한 정보를 여러 경로에서 수집하고 문서화하는 표준정보포맷이다. 문서화된 COBie 정보는 엔지니어가 사용하는 시설관리시스템과 데이터를 교환하는 수단으로 이용할 수 있다. 시설물 관리 데이터 교환 프로세스는 COBie를 사용하여 사용자가 필요로 하는 목록과 정보를 정형화한 후 정보를 입력한다. COBie는 설계 및 시공 단계에서 운영, 유지 보수, 자산 관리 등을 지원하기 위해 정보를 획득하고 업데이트하는 방식을 개선하기 위해 개발되었다. 하지만, COBie를 사용하기 위해서는 이기종 데이터 모델과 정보연계, 품질검수 등에 많은 수작업이 필요하다. 이런 이슈들은 COBie 기반 시설물 관리시스템 개발 시 장애요인이 된다. 이 연구는 시설물 관리를 위한 데이터 교환체계를 분석하여 BIM(Building Information Modeling)에 포함된 유지보수정보를 보다 쉽게 운영하기 위한 프레임 워크를 제안한다. 이 연구는 시설관리 정보제출 규칙, 품질점검, 프레임웍 컴포넌트 역할 정의, COBie 호환성 보장 및 정보연계 방안을 도출하였다. 프로토타입 개발을 통해 제시된 프레임웍을 기반으로 한 COBie DB 스키마 생성 시 효과적인 데이터 연계가 가능함을 확인하였다.