• 약학회지
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• 제55권2호
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• pp.138-144
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• 2011

The aim of present study was to investigate the possible influence and related mechanism of resveratrol on U-46619 (high concentration)-induced vasoconstriction. Agonist-induced vascular smooth muscle contractions involve the activation of thick or thin filament pathway. However, there are no reports addressing the question whether this pathway is involved in resveratrol-induced relaxation in rat aortae contracted with high U-46619. We hypothesized that MEK or Rho-kinase inhibition plays a role in vascular relaxation evoked by resveratrol in rat aortae. Endothelium-denuded arterial rings from male Sprague-Dawley rats were used and isometric contractions were recorded using a computerized data acquisition system. Resveratrol fully inhibited U-46619 in low concentration-induced contraction regardless of endothelial function. However, resveratrol partially decreased U-46619 in high concentration-induced contraction regardless of endothelial function. Interestingly, only in U-46619 (high concentration)-induced contraction, no significant decrease was observed in phospho-ERK1/2 levels and slight decrease in phospho-MYPT1 levels suggesting that additional pathways different from them or endothelial nitric oxide synthesis might be involved in the vasorelaxation. In conclusion, in high U-46619-contracted rat aortae, resveratrol showed relaxation response regardless of endothelial function significantly but slightly decreasing MYPT1 phosphorylation rather than ERK1/2 phosphorylation.

• 동의생리병리학회지
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• 제26권4호
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• pp.566-571
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• 2012

The purpose of this study was to assess the effects of herbs aroma on relaxation in highly stressed volunteers with heart rate variability(HRV) and SACL(stress arousal check list). 28 highly stressed volunteers participated in this study. The volunteers were examined with heart rate variability(HRV) and SACL(stress arousal check list) before and after smell herbs aroma. After smelling powder incense composed with oriental medicine herbs, mean RR, SDNN, LF, HF, TP were incresed and mean HRV, LF/HF ratio were decreased, but it was not significant. And VLF, norm HF were incresed and norm LF was decreased significantly. This results show that the powder incense composed with oriental medicine herbs effects on decresing sympathetic nervous activity and increasing parasympathetic activity and rebalancing autonomic nervous system who were under stress.

• 생명과학회지
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• 제10권6호
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• pp.584-590
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• 2000

Natural products are one of the useful source of cardiovascular drugs, in particular, when they have antioxidant activity. Gagaminine, an alkaloid isolated from the roots of Cynanchum wilfordi Hemsley, has been reported to potently inhibit the aldehyde oxidase activity ({TEX}$IC_{50}${/TEX}=0.8$\mu$M) and reduce lipid peroxidation. However, the effect of gagaminine on vascular smooth muscle has not yet been investigated. In the present study, we examined whether gagaminine relaxes vascular smooth muscle by isometric tension study. In order to observe its relaxation effect on the arteries, conductivel vessel (rat thoracic aorta) and resistance vessel (pig coronary artery) were purposely used. Results indicated that gagaminine relaxed in a concentration-dependent manner $\alpha$-adrenoceptor agonist, phenylephrine (PE)-induced contraction of rat aorta. Pretreatment with gagaminine inhibited PE-induced contraction, noncompetitively. {TEX}$Ca^{2+}${/TEX}-induced contraction was significantly diminished by gagaminine. In pig coronary artery, gagaminine relaxed thromboxane receptor (U 46619)-mediated contraction in dose-dependent manner. Pretreatment with gagaminine also reduced the maximum contraction induced by KCl. These observations strongly suggest that agagminnine relaxes vascular smooth muscle, irrespective of both resistance and conductive artery. We demonstrate that gagaminine, a potent natural antioxidant, has a significant vasodilatory effect and its action mechanism van be ascribed at least in part to {TEX}$Ca^{2+}${/TEX} antagonistic action as evidenced by inhibition {TEX}$Ca^{2+}${/TEX}-induced contraction (rat aorta) and KCl-induced contraction (porcine artery). Furthermore, neither $\alpha$ -adrenoceptor nor thromboxane receptor seems responsible for the relaxation of gagaminine.

• 인간식물환경학회지
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• 제22권3호
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• pp.255-268
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• 2019

Horticultural therapy, as a kind of complementary alternative therapies using nature as a medium, is an intervention method that can be applied to various subjects by utilizing horticultural activities that anyone can enjoy as a leisure activity. This research defined the resilience of individuals as a personal characteristic, and examined differences in the intervention effect of horticultural activities depending on the level of resilience. The results obtained in this study can be utilized in planning a horticultural activity program and setting the purpose and goals of horticultural activity programs. The subjects of this study were divided into the high resilience experimental group (Group A), the low resilience experimental Group (Group C), the high resilience control group (Group B), and the low resilience control group (Group D). The experiment was conducted in the campus of G University from September to November 2017, and the experimental group participated in the program once per week, a total of 10 sessions. The Korean version of the Connor-Davidson Resilience Scale, autonomic nervous assessment, and the interpersonal relationship change scale were carried out as pre- and post-assessment. Statistical analysis was performed using a non-parametric test. Group A showed statistically significant positive changes in relaxation of physical tension and stability. In conclusion, those with high resilience showed the higher intervention effects of horticultural activities on physical relaxation and stability than those with low resilience. However, there were some possible limitations in this study. Since the number of subjects was small and subjects were limited to college students, it is impossible to generalize the results of this study. Therefore, it is necessary to conduct follow-up studies to address and overcome these limitations.

• 한국산림과학회지
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• 제109권2호
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• pp.231-242
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• 2020

본 연구는 「국민 산림휴양·복지활동 실태조사(N=6,191)」 자료를 토대로 산림여가활동을 유형화하고 특성을 파악하였고, 산림여가활동 유형의 인구통계학적 영향요인을 분석하였다. 분석결과, 첫째, 방문목적을 기준으로 산림여가활동을 유형화한 결과, 4가지 유형(건강증진형, 교육학습형, 휴식·휴양형, 레포츠형)으로 분류되었다. 휴식·휴양형의 경우 참여자들은 다양한 계절에 방문하는 반면, 레포츠형의 경우, 여름에 주로 다양한 즐길거리를 고려하여 방문지를 선택한다는 점과 소요비용이 가장 높고 당일형/숙박형 활동일수가 가장 적다는 것이 특징적이라고 할 수 있다. 둘째, 산림여가활동 유형의 인구통계학적 영향요인을 분석한 결과, 연령, 성별, 자녀유무, 가계소득이 유형별 산림여가활동을 선택하는 데 영향을 미쳤으며 이 중 연령이 가장 큰 영향요인인 것으로 분석되었다. 연령이 높을수록 교육학습형과 레포츠형보다는 휴식·휴양형을, 휴식·휴양형보다는 건강증진형을 선호하는 것으로 나타났다. 남자인 경우 휴식·휴양형보다는 건강증진형을, 자녀가 있는 경우 휴식·휴양형보다는 교육학습형을 선택할 확률이 높았다. 또한 가계소득이 높을수록 교육학습형보다는 휴식휴양형을, 휴식휴양형보다는 건강증진형을 선호할 확률이 높은 것으로 분석되었다. 본 연구는 전국민을 대상으로 유형별 산림여가활동 선호에 연령이 가장 중요한 요소임을 전국단위에서 통계적으로 규명하였다는 데 의의가 있다. 향후 연구에서는 당일형과 숙박형을 구분하여 각각의 세부특성을 파악한다면 수요자 관점에서 산림복지에 대한 정책 방향을 설정하는데 기여할 수 있을 것으로 판단된다.

• Journal of Ginseng Research
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• 제34권4호
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• pp.314-320
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• 2010

The previous reports have showed that ginseng saponins, which are the active ingredients of Panax ginseng, cause the relaxation of artery that are contracted due to a various of hormones or potassium ($K^+$). Recently, we also showed that ginsenosides differentially regulate channel activity. The purpose of this study was to examine whether ginseng saponins affect contraction induced by $K^+$, serotonin (5-HT), or acetylcholine (Ach) in porcine coronary vessel. Treatment with concentrations of ginseng saponins caused a relaxation of 25 mM KCl-induced porcine coronary artery contraction. Also, ginseng saponin induced a significant dose-dependent relaxation of $3\;{\mu}M$ 5-HT-induced porcine coronary artery with the endothelium. In the porcine artery with the endothelium, ginseng saponins induced a relaxation by $3\;{\mu}M$ 5-HT in a concentration-dependent pattern. Ginseng saponins induced relaxation of both 25 mM KCl- and $3\;{\mu}M$ 5-HT-induced coronary artery contraction in the absence and presence of the endothelium. In contrast, treatment with $100\;{\mu}g/mL$ ginseng saponin did not induce relaxation in coronary artery contraction induced by Ach ($0.01\;{\mu}M$ to $30\;{\mu}M$) in the presence of the endothelium, but did cause significant relaxation of coronary artery contractions by Ach ($0.01\;{\mu}M$ to $30\;{\mu}M$) in the absence of the endothelium. These findings indicate that ginseng saponin (> $100\;{\mu}g/mL$) significantly inhibits porcine coronary artery contractions caused by $K^+$, 5-HT, and Ach. Therefore, in this study, we demonstrated that ginseng saponin may show beneficial roles on abnormal coronary contraction.

• 사상체질의학회지
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• 제2권1호
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• pp.123-134
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• 1990

In order to investigate experimentally the clinical effects of Taeuminkalkunhaeki-tang (太陰人葛根解肌湯) that was prescribed to cure the Kansuyolriyolbyong (肝受熱裏熱病) of Taeumin, the author experimented various activities of mixed extract from Taeuminkalkunhaeki-tang by the methods prescribed in the experimental part. The results of the studies were summarized as follows: 1. Sedative effects such as malfunction of the spontaneous motor activity in the wheel cage method experiment and muscle relaxation in the rotor rod method experiment were noted. 2. In mice, the prolongation of the duration of hypnosis Induced by thiopental-Na was noted. 3. In the acetic acid method experiment and the pressing hind paw method experiment, analgesic effects were noted. 4. Inhibitory effects were noted on the convulsions incuced by strychine and picrotoxin. 5. The expansion of blood vessels by relaxation of smooth muscle was noted. According to the above results, effects based on oriental medical references approximate to the actual experimental results.

• 한국환경보건학회지
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• 제26권4호
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• pp.115-121
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• 2000

To evaluate the cytotoxicity of cadmium compounds, this study was conducted to measure the in vitro magnetometry, LDH release and cellular apoptosis using alveolar macrophages of hamsters. A series of magnetometric measurements in cadmium-added groups showed a significant dose-dependent decay of the relaxation curves. The LDH release rates showed a dose-dependently increasing tendency as the dose gradually increased. The positive rates of apoptosis were significantly higher in cadmium-added groups than the control groups. Conclusively, the cytotoxicity increased in a dose dependent way as the concentration of cadmium added increased, which reflected in the decay of relaxation curve in magnetometry, and increased LDH release rate and positive rate of apoptosis.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.879-884
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• 2002

Previous study showed that an amidrazonophenylalanine derivative, LB30057, which has high water solubility, inhibited the catalytic activity of thrombin potently by interaction with the active site of thrombin. In the current investigation, we examined whether LB30057 inhibited platelet aggregation and vascular relaxation induced by thrombin. Treatment with LB30057 to plateletrich plasma (PRP) isolated from human blood resulted in a concentration-dependent inhibition of thrombin-induced aggregation. Values for $IC_{50}$ and $IC_{100}$ were $54{\pm}4$ nM and $96{\pm}3$ nM, respectively. This inhibition was agonist (thrombin) specific, since $IC_{50}$ values for collagen and ADP were \much greater than those for thrombin. In addition, concentration-dependent inhibitory effects were observed on the serotonin secretion induced by thrombin in PRP. Consistent with these findings, thrombin-induced increase in cytosolic calcium levels was inhibited in a concentration-dependent manner. When LB30057 was treated with aortic rings isolated from rats, LB30057 resulted in a concentration-dependent inhibition of thrombin-induced vascular relaxation. All these results suggest that LB30057 is a potent inhibitor of platelet aggregation and blood vessel relaxation induced by thrombin.

• 동의생리병리학회지
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• 제27권4호
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• pp.437-445
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• 2013

This study was investigated to evaluate the vasorelaxant effect of Rubus coreanus(RC) extract on contracted rabbit carotid artery and its mechanism. To study the effect of RC extract on contracted rabbit carotid arterial strips, arterial strips with intact or damaged endothelium were used for experiment using organ bath. The pre-contracted arterial strips with norepinephrine(NE) or potassium chloride(KCl) was treated with various concentrations of an extract of RC(0.01, 0.03, 0.1, 0.3 and 1.0 $mg/m{\ell}$). To determine the mechanisms of RC-induced vasorelaxant, RC extract was infused into contracted arterial rings which had been pretreated by indomethacin(IM), tetraethylammonium chloride(TEA), $N{\omega}$-nitro-L-arginine (L-NNA), methylene blue(MB). And calcium chloride(Ca) 1 mM was infused into precontracted arterial ring induced by NE or KCl after treatment of RC extract in $Ca^{2+}$-free krebs solution. Cytotoxic activity of RC extract on human umbilical vein endothelial cell(HUVEC) was measured by MTT assay, and nitric oxide(NO) prodution was measured by Griess reagent. RC extract revealed significant relaxation on NE-induced arterial contraction, but didn't relax on KCl-induced arterial contraction. RC extract also had an effective relaxation to the intact endothelium arterial ring, but not the damaged endothelium arterial ring. Treatment of IM, TEA, L-NNA, MB reduced the relaxation of RC extract. Pretreatment of RC extract inhibited the contraction by influx of extracellular $Ca^{2+}$ in contracted arterial ring induced by NE, but it didn't work the contraction by influx of extracellular $Ca^{2+}$ in contracted arterial ring induced by KCl in $Ca^{2+}$-free krebs solution. RC extract increased nitric oxide production on HUVEC. This study indicated that the relaxation effect of RC extract on contracted rabbit carotid artery is related with NO-cGMP pathway, EDHF, prostacyclin.