• 융합보안논문지
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• 제19권2호
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• pp.129-136
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• 2019

지상작전 시 지휘관 및 참모의 주된 업무는 지속적으로 발생되는 상황에 대처하기 위해 수행되는 의사결정의 연속이라고 볼 수 있다. 하지만 현재의 의사결정 과정은 지휘관 및 참모의 정성적인 방법에 의존하고 있기 때문에 제4차 산업혁명을 기반으로 현재 및 미래에 도입되고 있는 의사결정 도구와의 연결을 보장하기 어려운 실정이다. 본 연구에서는 지상작전과 연관된 의사결정 고려요소들의 정량화에 대해 전장정보분석(IPB) 시 접근로 평가요소들의 상대적 중요도를 AHP를 이용하여 도출하는 것을 중심으로 제시된다. 전장정보분석에서 가장 중요한 과정 중에 하나가 접근로에 대한 평가이며, 평가요소에는 목표 접근성 관측과 사계 은폐와 엄폐 장애물, 기동의 용이성 인접 접근로로의 전환 용이성을 포함하고 있다. 기존의 방식은 비교요소에 의한 방법과 장단점에 의한 비교가 있지만, 위 평가요소들의 중요도가 동등하게 적용된다는 문제점이 항상 제기되어 왔다. 전문가집단을 통한 분석적계층과정(AHP) 기법을 적용하였고, 분석결과 목표 접근성이 가장 중요한 평가요소임을 확인할 수 있었다.

• Food Science and Biotechnology
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• 제14권4호
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• pp.456-460
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• 2005

To enforce the maximum residue limit for residual hexane (0.005 g/kg) in commercially available Korean vegetable oil, convenient and accurate quantification methods were investigated. Using dual surrogate standards, pentane and heptane were dissolved in ethanol, and then added to hexane-tree sunflower oil for setting up the calibration curve. Gas Chromatograph-Flame Ionization Detector with a porous layer open tubular column, indicated good chromatographic separation of hexane from other inhibiting matrix components. The lowest calibration level was $0.5\;{\mu}g/g$, not exceeding a relative standard deviation of 10% (RSD%), and 1.0\;{\mu}g/g$ not exceeding a deviation of 22% RSD% using heptane as an internal standard for the Static headspace analysis by using a headspace auto-sampler and manual injection, respectively. The residual hexane was detected in nine of the samples among 87 vegetable oil samples purchased on the local market.

• Natural Product Sciences
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• 제24권3호
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• pp.206-212
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• 2018

Xanthii Fructus has been traditionally used for the treatment of rhinitis, rheumatoid arthritis, and eczema. In this study, a high-performance liquid chromatography-photodiode array (HPLC-PDA) method was developed and then used for the simultaneous analysis of eight phenylpropanoids in Xanthii Fructus. The analytical column used for this separation was a $SunFire^{TM}$ $C_{18}$ column, maintained at $40^{\circ}C$. The mobile phase used was 1.0% acetic acid in distilled water and 1.0% acetic acid in acetonitrile with gradient elution. For identify of each component, the mass spectrometer (MS) was used a Waters triple quadrupole mass spectrometer requipped with electrospray ionization (ESI) source. The HPLC-PDA method showed good linearity: correlation coefficients were ${\geq}0.9996$. The limits of detection and quantification of the eight compounds were 0.02 - 0.04 and $0.06-0.14{\mu}g/mL$, respectively. The extraction recoveries ranged from 97.51 to 108.67%. The relative standard deviation values of intra- and inter-day precision were 0.06 - 1.55 and 0.09 - 1.68%, respectively. The validated HPLC-PDA method was applied to simultaneously analyse the amounts of eight phenlypropanoids in Xanthii Fructus.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.750-753
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• 2001

Proteomics is a formalized approach for obtaining a rapid snap-shot of the protein complement of a tissue, cell or cell component. Such an approach is powerful in that it allows a parallel assessment of temporal protein fluxes. This is an important concept in view of the dynamic nature of protein expression. Undoubtedly, changes in protein expression are essential in any study aimed at investigating cellular networks. In this study, we analyzed and compared the proteomes of recombinant E. coli strain before and after induction. Proteome expression patterns of recombinant E. coli were resolved on 2D-gels, and the variations in the relative expression level of particular proteins were examined using software-aided protein quantification tool. We observed above 800 spots on a 2D-gel using Melanie II software. Many proteins which involved in chaperones were significantly up-regulated in recombinant E. coli. Therefore, it could be concluded that the expression of recombinant protein in E. coli acted as a stress to the cells, which change cells ability to synthesize proteins and induced the expression of various protective proteins.

• The Korean Journal of Ceramics
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• 제3권2호
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• pp.73-81
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• 1997

In this paper, it is intended to present more reproducible and quantitative method for adhesion assemssement. In scratch test, micromechanical analysis on the stress state beneath the indenter was carried out considering the additional blister field. The interface adhesion was quantified as work of adhesion through Griffith energy approach on the basis of the analyzed stress state. The work of adhesion for DLC film/WC-Co substrate calculated through the proposed analysis shows the identical value regardless of distinctly different critical loads measured with the change of film thickness and scratching speed. On the other hand, uniaxial loading was imposed on DCL film/Al substrate, developing the transverse film cracks perpendicular to loading direction. Since this film cracking behavior depends on the relative magnitude of adhesion strength to film fracture strength, the quantification of adhesion strength was given a trial through the micromechanical analysis of adhesion-dependence of film cracking patterns. The interface shear strength can be quantified from the measurement of strain $\varepsilon$s and crack spacing $\lambda$ at the cessation of film cracking.

• 농촌계획
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• 제16권3호
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• pp.27-41
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• 2010

This study developed a settlement environment diagnostic indices (SEDI) of rural villages which can apply to analyze variable conditions of villages having development projects. The index consists of 5 diagnostic areas (first level) with 13 items (second level), including detail indices of 40 criteria (third level) defined by specialists' brainstorming process. In order to develop the index system, object-oriented approach was used to extract the 5 diagnostic areas (infra-basic settlement facility, life condition & environmental resources, industry & economic infrastructure, community, and residents) from rural villages. For the 40 criteria, it was introduced a quantification method that all villages have absolute values in national level, not relative value between villages within an unit area. The diagnostic diagram and table were developed to evaluate the villages compositively. The developed SEDI was applied to study areas, 56 villages in 2 subdivision areas of county, located on county of Keumsan. Applicability of the index system, database system should be developed with the surveying method of data in village level.

• 대한한의학방제학회지
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• 제18권1호
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• pp.95-103
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• 2010

Objectives : To develop and validate HPLC-PDA methods for simultaneous determination of seven constituents in Samsoeum(SSE). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 254 and 280 nm, were used for quantification of the seven marker components of SSE. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results : Calibration curves were acquired with $r^2$>0.9997, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each compound was in the range of 100.07-112.65%, with an RSD less than 4.0%. The contents of seven compounds in SSE were 1.24-10.53 mg/g. Conclusions : The established method will be helpful to improve quality control of SSE.

• 한국대기환경학회지
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• 제21권6호
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• pp.593-603
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• 2005

Passive sampler using 4-amino-3-hydrazino-5-mercapto-1, 2, 4-triazole (AHMT) was developed to determine formaldehyde in indoor environment. The chromatography paper cleaned by $3\%$ hydrogen peroxide solution was experimently determined as a optimum absorbtion filter for the collection of formaldehyde. The passive sampler with a broad cross-sectional area and a short diffusion length was quite good in sensitivity. The passive sampler and the active sampling method with an impinger were strongly correlated with a correlation coefficient of 0.9848. The limits of detection and quantification of the passive sampler for the measurement of formaldehyde in the indoor environment were 7.5 and 10.2 ppb, respectively. Temperature ($19\∼28^{circ}C$) and relative humidity ($30\∼90\%$) had slight influence on the sampling rate of the passive sampler. However, the increase of flow velocity on the surface of sampler resulted in the increase of sampling rate.

• Journal of the Korean Wood Science and Technology
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• 제48권3호
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• pp.364-375
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• 2020

In this study, ultrasound-assisted extraction was performed to extract ascorbic acid from rugosa rose (Rosa rugosa Thunb.) fruit. The optimal conditions were investigated by response surface methodology, using two variable including reaction time (16-44 min) and temperature (16-44℃). The ascorbic acid extraction was sensitive to the reaction time rather than the reaction temperature, and the optimal conditions for ascorbic acid extraction were 25℃ and 30 min. Ascorbic acid and gallic acid in the rugosa rose fruit extract were completely separated by HPLC, with a resolution factor of over 1.5 between the two. The correlation coefficient of the ascorbic acid was 0.999 in a linearity test for 50-150 ㎍/mL concentration of extract. The limit of detection and limit of quantification values were 0.16 ㎍/mL and 29.89 ㎍/mL, respectively. The relative standard deviations (RSD) for repeatability and reproducibility were determined, and each RSD showed good precision at less than 5% (N=6).

• 생약학회지
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• 제40권3호
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• pp.224-228
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• 2009

For the quality control of traditional herbal medicine, Galgeun tang, simultaneous determination of glycyrrhizin, paeoniflorin, puerarin, 6-gingerol was established by using a high performance liquid chromatographic (HPLC) method with diode array detector. To separate five four constituents, DIONEX $C_{18}$ column ($5{\mu}m$, $120{\AA}$, $4.6\;mm{\times}150\;mm$) was used with gradient elution system of water and methanol. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curve of standard components showed excellent linearity ($R^2$>0.9906). Limits of detection (LOD) and limits of quantification (LOQ) varied from 0.15 to $0.52{\mu}g$/ml and 0.27 to $0.80{\mu}mg$/ml, respectively. The relative standard deviations (RSDs) of data of the intra-day and inter-day experiments were less than 2.88% and 1.21%, respectively. The results of recovery test were ranged from 96.71 to 106.29% with RSD values 0.01-0.80%.