• Journal of Ginseng Research
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• v.42 no.2
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• pp.149-157
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• 2018

Background: Panax notoginseng leaves (PNL) exhibit extensive activities, but few analytical methods have been established to exclusively determine the dammarane triterpene saponins in PNL. Methods: Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF MS) and HPLC-UV methods were developed for the qualitative and quantitative analysis of ginsenosides in PNL, respectively. Results: Extraction conditions, including solvents and extraction methods, were optimized, which showed that ginsenosides Rc and Rb3, the main components of PNL, are transformed to notoginsenosides Fe and Fd, respectively, in the presence of water, by removing a glucose residue from position C-3 via possible enzymatic hydrolysis. A total of 57 saponins were identified in the methanolic extract of PNL by UPLC/Q-TOF MS. Among them, 19 components were unambiguously characterized by their reference substances. Additionally, seven saponins of PNL-ginsenosides Rb1, Rc, Rb2, and Rb3, and notoginsenosides Fc, Fe, and Fd-were quantified using the HPLC-UV method after extraction with methanol. The separation of analytes, particularly the separation of notoginsenoside Fc and ginsenoside Rc, was achieved on a Zorbax ODS C8 column at a temperature of $35^{\circ}C$. This developed HPLC-UV method provides an adequate linearity ($r^2$ > 0.999), repeatability (relative standard deviation, RSD < 2.98%), and inter- and intraday variations (RSD < 4.40%) with recovery (98.7-106.1%) of seven saponins concerned. This validated method was also conducted to determine seven components in 10 batches of PNL. Conclusion: These findings are beneficial to the quality control of PNL and its relevant products.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.57-66
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• 2003

Single and 28-day repeated dose toxicity studies of botulimnn toxin type A were carried out in ICR mice and Sprague-Dawley rats, respectively. In the single dose toxicity study, botulinwn toxin was injected intraperitoneally to male and female mice at a single dose of 40, 59, 89 133 and 200 ng/10 ml saline/kg. All animals died from 59 ng/kg group. Some clinical signs, such as decrease in locomotor activity, dyspnea, prone position and ptosis, were observed in most of both sexes from 59 ng/kg group, but no signs were seen in all animals at 40 ng/kg group. The results showed that the median lethal dose of botulinum toxin might be in the range of 40-59 ng/kg in both sexes. In the repeated dose toxicity study, the test material was administered intradermally for 28 days at doses of 0 (vehicle-treated control), 1.25, 2.5, 5.0 and $10.0ng/head/50{\mu}{\ell}$ saline in male and female rats. No test material-related changes were noted in survivals, clinical signs, food and water consumptions and gross finding in any group. Botulinum toxin treatment significantly decreased the body weight gain rate in male of 5.0 ng/head group and over and in female of 10.0 ng/head group compared to vehicle-treated control. One or more relative organ weights (i.e., spleen, thymus, liver and kidney) were increased significantly from 5.0 ng/head group compared to vehicle-treated control in both sexes. Serum biochemistry revealed increases in aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase, total protein and albumin in male, and increases in AST and ALT and decreases in $K^+$ and $Cl^-$ in female without dose-pendent manners. In the histopathological study, physical stimulation by needle caused slight inflammations of dennis. In addition, botulinum toxin treatment induced denervation of nerve cell and disuse of muscle, resulting in atrophy of skeletal muscle in both sexes from 2.5 ng/head group. When the antibodies to toxin were determined in all animals, a significant increase in serum antibodies was observed from 5.0 ng/head group. The results showed that the NOAEL of botulinum toxin might be 1.25 ng/head for 28-day repeated dose toxicity in rats.

• Journal of the Korean Wood Science and Technology
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• v.16 no.2
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• pp.79-89
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• 1988

This experiment was carried out to investigate the difference of compositions between petroleum ether extract and its tall oil in Pinus rigida Mill. xylem and to obtain the basic data on effective extraction and its utilization of by-product, resin and fatty acid, when pulp making. After both petroleum ether extract from wood meal and its tall oil in pitch pine were separated to the resin and fatty acid by using DEAE-Sephadex and aluminum oxide column, these were analyzed with gas chromatograph. The results obtained were as follows: 1. Fatty acids contained as ester form in living trees were varied by peroxide of organic solvent in petroleum ehter extraction and cooking chemicals in cooking. 2. Pimaric-type acid was eluted earlier than abietic-type acid in resin acids and the fewer the carbons, the earlier the elution in fatty acids. 3. The retention time of SE-30 column was even smaller than that of OV-101 column but the relative retention time obtained by using methyl pimarate and methyl stearate as internal standard was nearly identical. 4. Both petroleum ether extract and tall oil mainly consisted of resin acids, expecially abietic-type acid. 5. Tall oil had more fatty acid but less resin acid than petroleum ether extract. Also, the content of unidentified materials was increased owing to the isomerization and the shift of double bond position in unsaturated fatty acids by high temperature and cooking chemicals when cooking.

• Journal of Korean Academy of Nursing
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• v.26 no.1
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• pp.94-106
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• 1996

Nursing literature suggests that the self-concept of nurses gives an important implications to educators and administrators as well as clinicians for their professional development. With a view to exploring how nurses view themselves, the Professional Self-Concept of Nurses Instrument (PSCNI) using 27 Likert items was developed by Arthur in Australia in 1990. This study is an extension of the PSCNI using Korean samples with some modifications. A convenience sample was drawn from 800 nurses working in three university hospitals in Seoul ; three university hospitals in Kyonggi-do and a university hospital and a psychiatric hospital in Kangwon-do. Seven hundred questionnaires were analyzed using the statistical analysis system(SAS). The reliability of the scale was tested by test-retest and Cronbach's alpha. Differences in the PSCNI, which are closely related to demographic variables, were examined by t-test, ANOVA and the Duncan's multiple range test. Factor analysis was employed so as to examine component factors. The results are summarized as follows : 1. The test-retest reliability of the PSCNI was .79 and Cronbach's alpha was .85. Item correlations with total revealed consistent correlations and subscale reliability varied from .49 to .85. 2. The average score of PSCNI was 75.21 and average item score was 2.79. 3. Twenty four items were derived from the PSCNI 27 items and these items clustered in three component factors. The cumulative percent of variance was 38.12％ and for factor 1 was 22.81％, for factor 2, 9.79％ and for factor 3, 5.51％ respectively 4. A comparison of the scores for the dimensions of the PSCNI shows a relative difference in terms of mean item scores and in descending order, professional practice（m=2.83）, communication （m=2.82） and satisfaction（m=2.70）. 5. Professional self-concept of nurses was found to vary significantly according to age（P=.0001）, religion（P=.0001）, academic background（P= .0109）, marital status（P=.0001）, career（P= .0001） and position（P=.0001）. In conclusion, there was a correlation between professional self-concept of nurses, and life and work variables. This study provides an important message for administrators and nurse educators by highlighting factors which can be addressed by education programs, staff development and appraisal. While the test results largely confirm the Australian and Canadian tests, further research is necessary to improve the cumulative percent of variance instead of applying Arthur's PSCNI directly to Korean nurses.

• The korean journal of orthodontics
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• v.25 no.2 s.49
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• pp.153-163
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• 1995

In this study, Receiver Operating Characteristic(ROC) analysis was used to evaluate the ability of cephalometric measurements to identify patients with Class III malocclusions. ROC analysis is the method for determining the validity of a diagnostic measure and for evaluating the relative value of diagnostic tests. The sample consisted of 496 patients with malocclusion. Class III malocclusion is defined as the dental relationship for which The mesiobuccal groove of the lower first molar is deviated mesially from the mesiobuccal cusp of the upper first molar. Of the total sample of 496 patients, 245 had Class III malocclusions. 16 cephalometric measurements were selected, each of which was treated as a diagnostic test. The ROC curves were generated for each cephalometric measurement with intervals of $1.0^{\circ}$ for angular measurements, 1.0mm for linear measurements. The area under the ROC curves was measured for direct comparison among different diagnostic tests. The results were as follows; 1. The 'Wits' appraisal was found to be a better diagnostic criterion for the presence of Class III malocclusion than any other commonly'used cephalometric measurement. 2. AB plane angle, ANB angle, App-Bpp distance, AF-BF distance, APDI, Distance of point A and Pog to N perpendicular, maxillomandibular differential had high diagnostic value. 3. Cephalometric measurements which evaluate the position of the mandible had moderate diagnostic value. 4. Cephalometric measurements related to the maxilla discriminated least between patients with and without Class III malocclusion.

• The korean journal of orthodontics
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• v.29 no.1 s.72
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• pp.23-35
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• 1999

Lateral cephalograms or 251 males md 286 females were taken and pubertal growth pattern or cranial base, maxillary and mandible of 7 to 17 years old Korean children was evaluated. 10 landmarks and 16 analytical measurements were evaluated. Analytical measurement and annual difference for each age group was calculated and tested for statistical significance. Analytical measurements were classified into three groups which were cranial base, maxillary and mandibular measurements and also classified into make and female measurements. Following results were achieved. 1. The circumpuberal growth spurt was earlier in Korean females than in males. 2. Cranial base, maxilla and mandible showed circumpuberal growth. The cranial base showed a relatively smaller amount of growth than the facial complex. 3. Middle and posterior cranial base length showed a floater increase than anterior cranial base length and circumpuberal growth spurt was also more definite. 4. the forward and downward growth or maxilla results from maxillary growht itself and transposition or the maxilla due to circumsutural growth aroud the maxilla. Ar-ANS and Ar-Pr which represent maxillary position relative to the cranial base showed more growth than ANS-PNS which represents maxillary bone growth. 5. mandible showed more vertical growth than horizontal growth but without significance. 6. Alveolar gwoth of maxilla and mandible show maximum growth rate of the time of permanent teeth eruption following loss of deciduous teeth . After this period alveolar growth shows a decreasing tendency.

• Physical Therapy Korea
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• v.19 no.4
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• pp.24-31
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• 2012

The aim of this study was to investigate the effectiveness of self-stretching exercises for iliotibial band (ITB) (Side-lying; right hip and knee were flexed to support the pelvis while left hip was extended and adducted, Standing A; side-bending of the trunk on standing with crossed leg, Standing B; same as Standing A, except the hands were clasped overhead and shifted right side, and Standing C; same as Standing B, except moving the arms diagonally downward) to help determine the most effective self-stretching method to stretch ITB. Twenty-one healthy subjects who do not have ITB shortness from Yonsei University (14 men and 7 women) between the ages of 18 to 28 years voluntarily participated. Ultrasound was performed to measure the thickness of the ITB between the long axis of the ITB and the level parallel to the lateral femoral epicondyle during four self-stretching exercises. All data were found to approximate a normal distribution. We used a one-way repeated-measures analysis of variance (ANOVA) to compare the thickness of the ITB among all self-stretching exercises. The level of significance was set at ${\alpha}$=.05. The ANOVA was followed by Bonferroni's correction. The overall mean of ITB thickness was $1.14{\pm}.4$ mm (${\pm}$ standard deviation) in resting status. The change in the ITB thickness in percentages between the tested position of each self-stretching exercises and resting status was significant (p<.05) (Side-lying $26.62{\pm}10.18%$ with 95% confidence interval [CI]=21.99~31.25%; Standing A $29.46{\pm}16.19%$ with 95% CI=22.09~36.84%; Standing B $44.06{\pm}14.82%$ with 95% CI=37.31~50.81%; Standing C $53.76{\pm}12.1%$ with 95% CI=48.25~59.29%). Results indicated significant differences among four self-stretching exercises except Side-lying versus Standing A (p<.01). Based on these findings, the Standing C self-stretching exercise was the most effective in stretching the ITB thickness among four types of ITB self-stretching exercises. Additionally, the Side-lying self-stretching exercise using gravity to stretch the ITB is recommended as a low-load (low-intensity), long-duration stretch.

• Journal of Korean Elementary Science Education
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• v.27 no.4
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• pp.385-398
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• 2008

The purpose of this study is to understand the effect of The Model of Phases of the Moon on conception changes for preservice teachers. The researcher interviewed two preservice teachers under the agreement with them on their participation in the research just before he performed a class using The New Model of Phases of the Moon. The post-interview with the same content as the pre-interview was preformed one month later. The main content of the interview is as follows; 'Explain the shape of the Moon by drawing it.', 'Explain the relative different position among the Sun, Earth, and Moon depending on phases of the Moon by drawing them.', 'What do you think of the cause of phases of the Moon?', 'Draw a picture to explain why we always see only one side of the moon.' The results of the research are as follows. First, the class with New Model of Phases of the Moon was able to perceive the relationship of Sun, Earth, and Moon in three-dimensions rather than in two-dimensions and it helped to change their misconception that the Moon's shadow causes the Moon's shape. Secondly, the class with New Model of Phases of the Moon helped preservice teachers understand better the different positional relationships among the Sun, Earth, and Moon depending on the Moon shapes. Third, the class adopting the New Model of Phases of the Moon help preservice teachers form scientific conceptions on the causes of phase change of the Moon. Fourth, the class with the New Model of Phases of the Moon is not appropriate for explaining the reason why only one face of the Moon is seen. Based upon the results above, the researcher realized the limitation of this model and suggested that this model would help learners understand phase change of the Moon and increase space perception ability.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.2
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• pp.145-152
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• 2010

This study evaluated the fatigue degradation in a SUS316L specimen using the nonlinear ultrasonic method. The nonlinearity of the ultrasonic wave was estimated by a relative nonlinear parameter defined as the ratio of the amplitudes for the fundamental wave to the second harmonic wave. In the experiment, a measurement system with contact transducers was constructed; reliable measurements were assured by keeping measurement conditions consistent and reducing extra harmonics generated in the measurement system. Two types of SUS316L specimen were used in experiments; a rotating bar fatigue specimen and a tensile fatigue specimen. The fatigue condition used was high cycle fatigue. The former specimen had a cylindrical shape and was used to observe the change in the nonlinear parameter after fatigue accumulation in a specimen. The latter was a plate-shaped specimen and was used to confirm the change in the nonlinear parameter at the position where the fatigue stress was concentrated. The measured nonlinear parameter showed a strong correlation to the damage level in both fatigue tests.

• The Korean Society of Law and Medicine
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• v.13 no.2
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• pp.11-38
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• 2012

The Supreme Court stand in the position in specific lawsuit that it doesn't allow the discretionary not covered service, but recently in revocation suit of fine disposal that is imposed on medical fee of leukemia patient, it altered the existing adjudgement and admitted the discretionary not covered service exceptionally. It put forward the allowable condition roughly in that case. According as this alteration, it has become more important to embody the allowance conditions of exceptions. The Supreme Court presented three things, which are procedural condition, medical condition and subscriber's agreement. Concerning procedural condition, several present conciliation procedures are as follows: medical care benefit arret request, relative value conciliation etc, prior request on anti-cancer drug among chemicals which exceed acceptance criteria, request of non benefit object on common drugs. To be granted the existence of those system, there should be no obstacle to use that. Even if it were so, we should take circumstances into consideration; individual situation is unescapable concerning substance and urgency of the discretionary not covered service, process of the procedure, time required etc. Regarding medical condition, safety and effectiveness will be verified through evaluation procedures of new medical skill. About the necessity, the Supreme Court made clear through a sentence that it allow the discretionary not covered service, in case that needs to treat a patient out of the standard of medical benefit. Strict interpretation is right and it answer the purpose of the sentence that the supreme court permit the discretionary not covered service, exceptionally. We need to differentiate medical necessity and medical validity. Subscriber's agreement should holds true if it entails full explanation, and if it is preliminary, explicit and individual. On this account, it should be difficult to admit that someone agree effectively when he call for the affirmation that he is recipient of medical care. Reasonable expense needs to be a part of review whether the agreement is valid. Meanwhile If we adjust system of medical expense and eventually reorganize a fee for consultation payment system (Fee-for-service controlled by item to DRG (Diagnosis Related Groups)), controversial area of the discretionary not covered service will be decreased and that will guarantee the discretion of the doctor.