• Journal of Nutrition and Health
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• v.36 no.2
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• pp.125-132
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• 2003

The present study was attempted to investigate and compare the antioxidant potency of several well-know flavonoids, antioxidant vitamin and commercially available popular beverages. The antioxidant potency was assessed by the effect on reducing oxidative DNA damage of human lymphocytes. Cellular oxidative DNA damage was measured by SCGE (single-cell gel electrophoresis), also known as comet assay. Lymphocytes were pre-treated for 30 minutes with wide ranges of doses of apigenin, kaempferol, luteolin, myricetin, rutin, quercetin, $\alpha$-tocopherol (10,25,50,100,200,500,1000 $\mu$M) ,green tea extract or grape juice (10,50,100,250,500,1000 $\mu$g/mL) followed by a $H_2O$$_2$(100 $\mu$M) treatment for 5 min as an oxidative stimulus. The physiological function of each antioxidant substance on oxidative DNA damage was analyzed as tail moment (tail length $\times$ percentage migrated DNA in tail) and expressed as relative DNA damage score after adjusting by the level of control treatment. Cells treated with $H_2O$$_2$alone (positive control) had an extensive DNA damage compared with cells treated with phosphate buffered saline (PBS, negative control) or pre-treated with all the tested samples. Of all the six flavonoids, quercetin was the most potent antioxidant showing the lowest $ED_{50}$/ of 8.5 $\mu$g/mL (concentration to produce 50% protection of relative DNA damage). The antoxidant potency of individual flavonoids were ranked as follows in a decreasing order; luteolin (18.4 $\mu$g/mL), myricetin (19.0 $\mu$g/mL) , rutin (22.2 $\mu$g/mL) , apigenin (24,3 $\mu$g/mL) , kaempferol (25.5 $\mu$g/mL). The protective effect of $\alpha$-tocopherol was substantially lower (highest $ED_{50}$value of 55.0 $\mu$g/mL) than all the other flavonoids, while the protective effect was highest in green tea and grape juice with low ED5O value of 7.6 and 5.3, respectively. These results suggest that flavonoids, especially quercetin, and natural compounds from food product, green tea and grape juice, produced powerful anti-oxidative activities, even stronger than $\alpha$-tocopherol. Taken together, supplementation of antioxidants to lymphocytes followed by oxidative stimulus inhibited damage to cellular DNA, supporting a protective effect against oxidative damage induced by reactive oxygen species.

• Smart Structures and Systems
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• v.15 no.3
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• pp.699-715
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• 2015

A modal parameter based damage sensitive feature (DSF) is defined to mimic the relative change in any diagonal element of the stiffness matrix of a model of a structure. The damage assessment is performed in a statistical pattern recognition framework using empirical complementary cumulative distribution functions (ECCDFs) of the DSFs extracted from measured operational vibration response data. Methods are discussed to perform probabilistic structural health assessment with respect to the following questions: (a) "Is there a change in the current state of the structure compared to the baseline state?", (b) "Does the change indicate a localized stiffness reduction or increase?", with the latter representing a situation of retrofitting operations, and (c) "What is the severity of the change in a probabilistic sense?". To identify a range of normal structural variations due to environmental and operational conditions, lower and upper bound ECCDFs are used to define the baseline structural state. Such an approach attempts to decouple "non-damage" related variations from damage induced changes, and account for the unknown environmental/operational conditions of the current state. The damage assessment procedure is discussed using numerical simulations of ambient vibration testing of a bridge deck system, as well as shake table experimental data from a 4-story steel frame.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1999.06a
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• pp.53-60
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• 1999

As the flying height decreased, it is essential that hard disk drives perform reliable under various environmental conditions. In this paper the tribological characteristics of a head/disk interface are investigated under various temperature, relative humidity, altitude and outgas conditions. Specially, Contact-Start-Stop(CSS) tests were performed to assess the stiction, acoustic emission, slider take-off behavior, and track average amplitude(TAA). It is shown that the surface damage and head failure are accelerated by high temperature and humidity as well as low ambient pressure.

• Toxicological Research
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• v.7 no.1
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• pp.83-92
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• 1991

Complexities of testis structure and function are emphasized in morphometrical and genotoxic evaluation by statistical analysis. F-344 rats were treated with azinphos methyl, cyclophosphomide, and dichlorvos. And Brdu was injected with intrapertionially before sacrifice. The existence and degree of DNA damage were measured by Brdu labeling index which represented relative amount of Brdu incorporated in DNA, morphometric change was evaluated by the relative length of tubular diameter in circular seminiferous tubules and the number of spermatogonia per Sertoli cell in stage IX seminiferous tubules.

• YAKHAK HOEJI
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• v.51 no.4
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• pp.239-245
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• 2007

In order to evaluate the genotoxicity of airborne particulate matter extracted with dichloromethane (APE), the rat microsome mediated (S-9) or DNA repair enzyme treated Comet assays were performed using the single cell gel electrophoresis in A549 human lung carcinoma cells. It was found that the cells interacting with APE showed more DNA single-strand breaks relative to untreated cells. The genotoxicity of APE was increased with the treatment of S-9 mixture. Microsome mediated DNA damage was inhibited by CYP1Al inhibitor, quercetin. The APE also showed oxidative DNA damage evaluated by endonuclease III treatment. Oxidative DNA damage of APE was inhibited by antioxidants such as vita- min C and Trolox. We also found that the vegetables or fruits extract may reduce APE-induced genotoxicity by their anti- oxidant activity and CYP1A1 inhibition.

• Proceedings of the Korean Geotechical Society Conference
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• 2004.03b
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• pp.765-774
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• 2004

This paper presents a damage assesment method for buried pipelines subjected to Deep Excavation-induced ground movements. Ground deformation characteristics resulting from 3D finite element analysis was represented mathematically by a hyperbolic tangential function. A parametric study was performed on excavation depth and burial position of pipeline. The result of the parametric study indicate that length of hyperbolic tangential function affects the results of damage assessment. Using numerical studies for buried pipeline response to ground movements by relative flexibility of the pipe-soil system. The result of numerical studies are presented in forms of design charts which can be readily used for various condition encountered in practices.

• Structural Engineering and Mechanics
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• v.24 no.2
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• pp.181-194
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• 2006

The masonry is a complex heterogeneous material and its shear deformation and fracture is associated with very complicated progressive failures in masonry structure, and is investigated in this paper using a mesoscopic mechanical modelling, Considering the heterogeneity of masonry material, based on the damage mechanics and elastic-brittle theory, the newly developed Material Failure Process Analysis (MFPA) system was brought out to simulate the cracking process of masonry, which was considered as a three-phase composite of the block phase, the mortar phase and the block-mortar interfaces. The crack propagation processes simulated with this model shows good agreement with those of experimental observations by other researchers. This finding indicates that the shear fracture of masonry observed at the macroscopic level is predominantly caused by tensile damage at the mesoscopic level. Some brittle materials are so weak in tension relative to shear that tensile rather than shear fractures are generated in pure shear loading.

• Journal of Conservation Science
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• v.30 no.2
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• pp.169-179
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• 2014

After occurrence of Cyanobacteria in 1997, Songsan-ri tombs located in Gonju have been investigated to monitor for biological damage. The room temperature of Tomb No.6 was $18.6{\sim}19.8^{\circ}C$ and the relative humidity was 94.3~99.9%. The temperature of Royal Tomb of King Muryeong was $17.3{\sim}18.53^{\circ}C$ and the relative humidity was 73.2~96.45%. The variation of relative humidity increased after setting up air vents. If the outside temperature increases, dew condensation occurs on the floor and the north side. When conditioning equipment operates, the maximum temperature differences between walls is $2.8^{\circ}C$. Bacteria from the air of the tomb and on the surface of the walls outnumbered fungi. 20 species of fungi including Alternaria sp., Aspergillus sp., Penicillium sp., and 19 species of bacteria including Pseudomonas sp., Arthrobacter sp., are identified. Microbes in the tombs may damage cultural heritage. The growth possibility of microbes should be estimated because the microbes in the tombs may damage mural painting. The interrelation between microenvironmental condition and biological damage of mural painting should be researched to come up with an long-term conservation method.

• Journal of the Earthquake Engineering Society of Korea
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• v.23 no.6
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• pp.329-339
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• 2019

The strategy for the management of earthquakes is shifting from post recovery to prevention; therefore, seismic performance management requires quantitative predictions of damage and the establishment of strategies for initial responses to earthquakes. Currently, seismic performance evaluation for seismic management in Korea consists of two stages: preliminary evaluation and detailed evaluation. Also, the priority of seismic performance management is determined in accordance with the preliminary evaluation. As a deterministic method, preliminary evaluation quantifies the physical condition and socio-economic importance of a facility by various predetermined indices, and the priority is decided by the relative value of the indices; however, with the deterministic method it is difficult to consider any uncertainty related to the return-year, epicenter, and propagation of seismic energy. Also this method cannot support tasks such as quantitative socio-economic damage and the provision of data for initial responses to earthquakes. Moreover, indirect damage is often greater than direct damage; therefore, a method to quantify damage is needed to enhance accuracy. In this paper, a Seismic Risk Assessment is used to quantify the cost of damage of road facilities in Pohang city and to support decision making.

• Journal of the Earthquake Engineering Society of Korea
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• v.23 no.5
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• pp.261-268
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• 2019

This paper proposes damage indices efficient on evaluating the seismic safety of cable-stayed bridges, especially dual-plane, cable-stayed bridges with H-type pylons. The research assumes that the location of accelerometers is already defined as given in the 2017 Ministry of the Interior and Safety (MOIS) guideline. In other words, the paper does not attempt to suggest optimal sensor location for the seismic safety evaluation of cable-stayed bridges. The proposed damage indices are based on those for building structures widely applied in the field already. Those include changes in natural frequencies and changes in relative lateral displacements. In addition, the study proposes other efficient damage indices as the rotation changes at the top of pylons and in the midspan of the girder system. Sensitivity analysis for various damage indices is performed through dynamic analysis using selected earthquake ground motions. The paper compares the effectiveness of the damage indices.