• Bulletin of the Korean Chemical Society
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• 제22권8호
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• pp.903-908
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• 2001

The gene aeg-46.5, which is expressed under anaerobic condition, has putative triple -10 regions and four transcription start sites. The mRNA transcription level and its start point change depending on the aerobic/anaerobic growth condition. RNA polymerase and its regulatory proteins must choose which of three -10 region to use. The putative triple 10 region was mutated to make only one of them function with consensus -10 region sequence (TATAAT) and the other two as non-functional region. The results show that the second and third -10 regions are used for the aerobic/anaerobic expression. The third -10 region is responsible for the high aerobic to anaerobic switch ratio. This suggests that only the last two of the putative triple -10 region have functions on aeg-46.5 gene expression switch control. The phenotype of the mutated promoter was tested in the wild type cell and narL - cell. The results indicate that the control by NarL is independent from the selection of -10 region. The expression patterns on multi-copy plasmids and on single-copy chromosome were compared. These results show that the aerobic/anaerobic switch control of aeg-46.5 is through the choice of -10 region. The mechanism of choosing different -10 region remains to be seen.

• Genomics & Informatics
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• 제9권3호
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• pp.127-133
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• 2011

The Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) is one of the major genomic resources for human genetics and immunological studies. Use of LCLs is currently extended to pharmacogenetic studies to investigate variations in human gene expression as well as drug responses between individuals. We evaluated four common internal controls for gene expression analysis of selected hematopoietic transcriptional regulatory genes between B cells and LCLs. In this study, the expression pattern analyses showed that TBP (TATA box-binding protein) is a suitable internal control for normalization, whereas GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is not a good internal control for gene expression analyses of hematopoiesis-related genes between B cells and LCLs at different subculture passages. Using the TBP normalizer, we found significant gene expression changes in selected hematopoietic transcriptional regulatory genes (downregulation of RUNX1, RUNX3, CBFB, TLE1, and NOTCH2 ; upregulation of MSC and PLAGL2) between B cells and LCLs at different passage numbers. These results suggest that these hematopoietic transcriptional regulatory genes are potential cellular targets of EBV infection, contributing to EBV-mediated B-cell transformation and LCL immortalization.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2001년도 춘계학술발표회요약집
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• pp.103.1-103
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• 2001

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2018년도 추계학술논문요약집
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• pp.51-52
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• 2018

• 방사성폐기물학회지
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• 제14권1호
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• pp.79-90
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• 2016

The second-stage near surface disposal facility for low and very low level radioactive waste's permanent disposal is to be built. During the institutional control period, the inadvertent intrusion of the general public is limited. But after the institutional control period, the access to the general public is not restricted. Therefore human who has purpose of residence and resource exploration can intrude the disposal facility. In this case, radioactive effects to the intruder should be limited within regulatory dose limits. This study conducted the safety assessment of human intrusion on the second-stage surface disposal facility through drilling and post drilling scenario. Results of drilling and post drilling scenario were satisfied with regulatory dose limits. The result showed that post-drilling scenario was more significant than drilling scenario. According to the human intrusion time and behavior after the closure of the facility, dominant radionuclide contributing to the intruder was different. Sensitivity analyses on the parameters about the human behavior were also satisfied with regulatory dose limits. Especially, manual redistribution factor was the most sensitive parameter on exposure dose. A loading plan of spent filter waste and dry active waste was more effective than a loading plan of spent filter waste and other wastes for the radiological point of view. These results can be expected to provide both robustness and defense in depth for the development of safety case further.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1986년도 한국자동제어학술회의논문집; 한국과학기술대학, 충남; 17-18 Oct. 1986
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• pp.205-208
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• 1986

The synthesis of control structure involves the selection of controlled variable (or measured variable), and the structure interconnecting measured and manipulated variables (control loops). This paper deals with the synthesis job by using the structural analysis and block relative gain. This synthesis tool is very useful because they require minimal information, and the results show that this is a systematic and efficient metholodgy.

• 정보보호학회논문지
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• 제29권5호
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• pp.1077-1088
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• 2019

원자력 발전소의 사이버위협이 현실화되면서 국제사회 및 국내에서는 사이버보안 규제지침 마련을 통해 필수디지털자산에 대한 적절한 보안조치를 적용하도록 요구하고 있다. 그러나 각 필수디지털자산에 대해 일괄적으로 동일한 사이버 보안조치 적용에 대해 규제 대상 내에서도 원전의 비상정지를 일으키고, 노심 손상을 유발할 수 있는 사고와 직접적으로 관련된 디지털자산에 단계적 접근방식을 적용한 규제 효과성 제고가 필요하다. 이에 본 연구에서는 원전 사고와 직접 관련된 디지털자산에 대하여 단계적 접근방식의 규제요건 개발을 위한 보안조치 적용 방안을 제시하였다. 단계적 접근방식의 기본적 고려사항인 규제 대상 설비의 침해영향도(Consequence)를 기반으로 한 규제 요건 적용을 위해 기존의 필수디지털자산에 요구되고 있는 보안조치를 보다 강화하거나 추가적인 보안조치를 개발하여 원전 사고와 직접 관련된 디지털자산에 요구하는 방식과 기존의 보안조치를 재분석하여 원전 사고와 직접 관련되지 않는 필수디지털자산에 대해서는 최소한의 보안조치를 요구하는 방식으로 크게 나누고 각 방안 별 세부적 사항을 기술하였다.

• BMB Reports
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• 제47권5호
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• pp.292-297
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• 2014

Age-related macular degeneration (AMD) is the leading cause of blindness in the world. Evidence indicates that the suppression of the ubiquitin-proteasome system (UPS) contributes to the accumulation of toxic proteins and inflammation in retinal pigment epithelium (RPE), the functional abnormalities and/or the degeneration of which are believed to be the initiators and major pathologies of AMD. To identify new protein associations with the altered UPS in AMD, we used LC-ESI-MS/MS to perform a proteomic analysis of the aqueous humor (AH) of AMD patients and matched control subjects. Six UPS-related proteins were present in the AH of the patients and control subjects. Four of the proteins, including 26S proteasome non-ATPase regulatory subunit 1 (Rpn2), were increased in patients, according to semi-quantitative proteomic profiling. An LC-MRM assay revealed a significant increase of Rpn2 in 15 AMD patients compared to the control subjects, suggesting that this protein could be a biomarker for AMD.

• Nuclear Engineering and Technology
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• 제52권5호
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• pp.995-1001
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• 2020

With the development of digital instrumentation and control (I&C) devices, cyber security at nuclear power plants (NPPs) has become a hot issue. The Stuxnet, which destroyed Iran's uranium enrichment facility in 2010, suggests that NPPs could even lead to an accident involving the release of radioactive materials cyber-attacks. However, cyber security research on industrial control systems (ICSs) and supervisory control and data acquisition (SCADA) systems is relatively inadequate compared to information technology (IT) and further it is difficult to study cyber-attack taxonomy for NPPs considering the characteristics of ICSs. The advanced research of cyber-attack taxonomy does not reflect the architectural and inherent characteristics of NPPs and lacks a systematic countermeasure strategy. Therefore, it is necessary to more systematically check the consistency of operators and regulators related to cyber security, as in regulatory guide 5.71 (RG.5.71) and regulatory standard 015 (RS.015). For this reason, this paper attempts to suggest a template for cyber-attack taxonomy based on the characteristics of NPPs and exemplifies a specific cyber-attack case in the template. In addition, this paper proposes a systematic countermeasure strategy by matching the countermeasure with critical digital assets (CDAs). The cyber-attack cases investigated using the proposed cyber-attack taxonomy can be used as data for evaluation and validation of cyber security conformance for digital devices to be applied, and as effective prevention and mitigation for cyber-attacks of NPPs.

• The Korean Journal of Physiology
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• 제30권2호
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• pp.231-236
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• 1996

In our previous study (Kim et al, 1991), GLUT 4 protein content correlated negatively with plasma glucose levels in skeletal muscles of STZ-induced diabetic rats. Thus, in this study, to confirm whether expression of GLUT 4 correlate negatively with degree of hyperglycemia, we measured levels of GLUT 4 mRNA in red and white gastrocnemius muscles in STZ-induced mild and severe diabetic rats. Rats were randomly assigned to control, mild, and severe diabetic groups, and the diabetes was induced by intraperitoneal administration of STZ. The experiment was carried out 10 days after STZ administration. Gastrocnemius red and white muscles were used fur the measurement of GLUT 4 expression. Plasma glucose levels of mild and severe diabetic rats were increased compared to control rats (control, mild, and severe diabetes; $6.4{\pm}0.32,\;9.4{\pm}0.68,\;and\;22.0{\pm}0.58$ mmol/L, respectively). Plasma insulin levels of mild and severe diabetic rats were decreased compared to control rats (control, mild, and severe diabetes; $198{\pm}37,\;l14{\pm}14,\;and\;90{\pm}15$ pmol/L, respectively). GLUT 4 mRNA levels of gastrocnemius red muscles in mild and severe diabetic rats were decreased compared to control rats ($64{\pm}1.2%\;and\;71{\pm}2.0%$ of control, respectively), but GLUT 4 mRNA levels in gastrocnemius white muscles were unaltered in diabetic rats. In summary, GLUT 4 mRNA levels were decreased in STZ-induced diabetic rats but did not correlated negatively with degree of hyperglycemia, and this result suggest that the regulatory mechanisms of decreased GLUT 4 mRNA levels are hypoinsulinemia and/or other metabolic factor but not hyperglycemia. And regulation of GLUT 4 expression in STZ-induced diabetes between red and white enriched skeletal muscles may be related to a fiber specific gene regulatory mechanism.