• 한국생물정보학회:학술대회논문집
• /
• 한국생물정보시스템생물학회 2004년도 The 3rd Annual Conference for The Korean Society for Bioinformatics Association of Asian Societies for Bioinformatics 2004 Symposium
• /
• pp.39-49
• /
• 2004

When there is a lack of detailed kinetic information, dFBA(dynamic flux balance analysis) has correctly predicted cellular behavior under given environmental conditions with FBA and different ial equations. However, until now, dFBA has centered on substrate concentration, cell growth, and gene on/off, but a detailed hierarchical structure of a regulatory network has not been taken into account. For this reason, the dFBA has limited the represen tation of interactions between specific regulatory proteins and genes and the whole transcriptional regulation mechanism with environmental change. Moreover, to calculate optimal metabolic flux distribution which maximizes the growth flux and predict the b ehavior of cell system, linear programming package(LINDO) and spreadsheet package(EXCEL) have been used simultaneously. thses two software package have limited in the visual representation of simulation results and it can be difficult for a user to look at the effects of changing inputs to the models. Here, we descirbes the construction of hierarchical regulatory network with defined symbolsand the development of an integrated system that can predict the total control mechanism of regulatory elements (opero ns, genes, effectors, etc.), substrate concentration, growth rate, and optimal flux distribution with time. All programming procedures were accoplished in a visual programming environment (LabVIEW).

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권4호
• /
• pp.500-508
• /
• 2011

Forty-four Gansu Alpine Fine-wool lambs were used to study changes in the activities of three gastric and five pancreatic enzymes under grazing conditions between 0 and 56 days of age. The lambs were slaughtered on days 0, 3, 7, 14, 21, 28, 42 and 56, the abomasal contents, mucosa and pancreas were immediately removed and placed into liquid nitrogen and enzyme activities were determined. Gastric enzyme (chymosin, pepsin and pregastrc esterase) activities were relatively high at birth, especially chymosin, but decreased quickly between day 0 and 21. The activity of pepsin changed insignificantly with increasing age. There was no significant change in the pancreatic enzyme activities (trypsin, chymotrypsin, ${\alpha}$-amylase, lipase and lactase). The activity of trypsin was relatively higher than that of the other pancreatic enzymes, and lactase activity was low. These ontogenic patterns might be under the control of many gut regulatory peptides, the plasma concentrations of which changed simultaneously. Some gastric and pancreatic enzymes were correlated with plasma concentrations of these gut regulatory peptides.

• Mycobiology
• /
• 제37권3호
• /
• pp.171-182
• /
• 2009

Many aspergilli that belongs to ascomycetes have sexuality. In a homothallic or self-fertile fungus, a number of fruiting bodies or cleistothecia are formed in a thallus grown from a single haploid conidia or ascospores. Genome-sequencing project revealed that two mating genes (MAT) encoding the regulatory proteins that are necessary for controlling partner recognition in heterothallic fungi were conserved in most aspergilli. The MAT gene products in some self-fertile species were not required for recognition of mating partner at pheromone-signaling stage but required at later stages of sexual development. Various environmental factors such as nutritional status, culture conditions and several stresses, influence the decision or progression of sexual reproduction. A large number of genes are expected to be involved in sexual development of Emericella nidulans (anamorph: Aspergillus nidulans), a genetic and biological model organism in aspergilli. The sexual development process can be grouped into several development stages, including the decision of sexual reproductive cycle, mating process, growth of fruiting body, karyogamy followed by meiosis, and sporulation process. Complicated regulatory networks, such as signal transduction pathways and gene expression controls, may work in each stage and stage-to-stage linkages. In this review, the components joining in the regulatory pathways of sexual development, although they constitute only a small part of the whole regulatory networks, are briefly mentioned. Some of them control sexual development positively and some do negatively. Regarding the difficulties for studying sexual differentiation compare to asexual one, recent progresses in molecular genetics of E. nidulans enlarge the boundaries of understanding sexual development in the non-fertile species as well as in fertile fungi.

• 미생물학회지
• /
• 제30권5호
• /
• pp.347-354
• /
• 1992

New regulatory, loci which participate in the regulation of anaerobic inducible gene expression in Salmonella typhimurium were identified. We observed the regulatory network of new regulator mutations to various anaerobic inducible gene (1). Some anaerobic inducible lac fusions were also induced at low pH condition which was severe environment to withstand for its virulence at the place like phagolysosome. Sic oxygen-regulated regulatory mutants (oxr) isolated by Tn10 mutagenesis were divided into two groups. Five of them were found to show negative effect on the regulation of anaerobic gene expression, while on e showed positive effect on the regulation. Genetic loci of four oxr were identified with 54 Mud-P22 lysogens covering the whole chromosome of S. typhimurium, in the nearby region of map unit 87 min (oxr101), 63 min (oxr104), 97 min (oxr 105), and 57 min (oxr 106), respectively. Two oxr mutants were subjected to two-dimensional polyacrylamide electrophoretic analysis of anaerobic inducible proteins for searching the control circuitry of our oxr mutants.

• 한국중재학회지:중재연구
• /
• 제14권1호
• /
• pp.185-211
• /
• 2004

It is a common and prevailing Construction that recent environmental problems such as earth's warmer climate and depletion of ozone layers can never be solved by the efforts of any one nation. That is why the international conventions have been held more often lately in order to discuss the matter of environment protection and there has been increased tendency of using economic activities of any nation especially their international trades as means for achieving the purpose of environment protection. Furthermore, there is an ample possibility for the advanced countries to use the regulatory restrictions for environment protection as an another measure of none-tariff barrier against countries including Korea which has high dependence on international trade with not very high technological capability of environment protection. Some of the developed countries have recently moved toward the creation of a new system of international regulatory measures on world trade in the name of Environment Protection. They Plan to impose strict control over the process and technology of production of good they are importing from other country. However, ever though the international regulatory measures, in a short term, could play a negative role on our trade capability, they could, in the long run, also play a role of helping hands if we usefully work out positive countermeasures as a result of hand-working government supported by industry and all the society as a whole.

• 한국전자통신학회논문지
• /
• 제12권5호
• /
• pp.725-730
• /
• 2017

원전의 안전 유지를 위한 계측제어계통에 일반적인 IT 자원을 활용하는 사례가 증가하고 있다. 이에 따라 기존 IT 자원이 갖는 잠정적인 보안 취약점으로 인해 원전 사이버 보안 침해 사고가 발생할 수 있으며, 원전의 가동 중단뿐만 아니라 국가적 재난에 이르는 심각한 사고를 야기할 수 있다는 문제가 제기되고 있다. 국내 원자력 규제기관에서는 이에 대응하기 위해 원전 사이버 보안 규제지침을 개발하고 규제 대상 및 범위를 확대시키고 있지만, 원전의 일반적인 보안 문제뿐만 아니라 원전 취약점에 특화된 공격에도 대응할 수 있는 방안이 필요하다. 이에 본 논문에서는 R.G.5.71에서 규정하고 있는 내용 중 취약점 점검과 관련된 42개 항목을 선별하여 5가지의 유형으로 분류 분석하였다. 제안된 분석 내용을 바탕으로 취약점 점검 도구를 개발한다면 원전 사이버 보안 취약점 점검 효율성을 향상시킬 수 있을 것으로 판단된다.

• 한국발생생물학회지:발생과생식
• /
• 제4권1호
• /
• pp.109-114
• /
• 2000

본 연구는 사춘기전과 사춘기가 시작될 무렵의 흰쥐난소에서 에탄올이 steroidogenic acute regulatory protein(StAR)의 유전자 발현에 어떠한 영향을 미치는지 조사하고자 수행되었다. 생후 25일 째부터 매일 흰쥐에 에탄을 또는 생리식염수를 복강주사하고 생후 27일, 32일 째에 실험동물을 희생시켜 혈액과 난소를 적출하였다. 실험결과 에탄올은 혈중의 황체호르몬 함량을 유의하게 감소시켰으며, 자궁의 무게는 27일 째에는 에탄올처리군에서 유의하게 적었으나, 32일 째에는 차이를 나타내지 않았다. 사춘기의 시작을 나타내는 지표의 하나인 viginal opening은 에탄올처리군에서 현저히 지연되었으며, 난소에서의 StAr mRNA발현 또한 유의하게 감소됨을 알 수 있었다. 따라서 본 연구결과는 에탄올이 적어도 사춘기 난소의 기능 및 사춘기의 시작을 교란시킬 수 있으며, 이러한 영향의 일부는 난소내 StAR유전자 발현을 억제함으로써 나타난다고 사료된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권4호
• /
• pp.2027-2030
• /
• 2016

Objective: To investigate the effect of peripheral blood CD4 + CD25 + regulatory T cell on postoperative immunotherapy in patients with renal carcinoma. Methods: 38 patients with renal cell carcinoma were recruited, and 20 patients from the operation group purely underwent the radical nephrectomy therapy, 18 patients from the combined group successively underwent the radical nephrectomy therapy and IFN-${\alpha}$ adjuvant immunotherapy. Additionally, 12 healthy subjects were recruited in the same period of time and regarded as the control group. Flow cytometry was used to detect CD4 +, CD8 +, CD4 + CD25+ T lymphocyte subset content and the ratio of all parts in the pre-operative period, in the first post-operative week and in the third post-operative month, compare and analyze its variation trend. Results: The CD4+CD25+ T lymphocyte subset content of individual renal carcinoma patients was significantly higher than that of the control group, also increases with the progression in the tumor stage (P<0.05). The post-operative CD4 + CD25+T lymphocytes of individual operation group and combined group patients showed different degrees of increment, but the increment of the combined group was significantly lower than that of the operation group (P<0.05). For the combined group patients with less pre-operative CD4 + CD25+T lymphocytes, their levels would increase after the immunotherapy, while the pre-operative patients with more CD4 + CD25+ T lymphocytes were the opposite situation. Conclusion: The detection of peripheral blood CD4+CD25+ regulatory T lymphocyte subset can reflect the anti-tumor immune status of renal cell carcinoma patient body. It can contribute to predict the prognosis of immunotherapy and provide reference for the choice of renal carcinoma post-operative adjuvant immunotherapy.

• Journal of Microbiology and Biotechnology
• /
• 제16권11호
• /
• pp.1661-1677
• /
• 2006

Rhizobium and related genera are soil bacteria with great metabolic plasticity. These microorganisms survive in many different environments and are capable of eliciting the formation of nitrogen-fixing nodules on legumes. The successful establishment of symbiosis is precisely regulated and requires a series of signal exchanges between the two partners. Quorum sensing (QS) is a prevalent form of population density-dependent gene regulation. Recently, increasing evidence indicates that rhizobial quorum sensing provides a pervasive regulatory network, which plays a more generalized role in the physiological activity of free-living rhizobia, as well as during symbiosis. Several rhizobia utilize multiple, overlapping quorum sensing systems to regulate diverse properties, including conjugal transfer and copy number control of plasmids, exopolysaccharide biosynthesis, rhizosphere-related functions, and cell growth. Genomic and proteomic analyses have begun to reveal the wide range of functions under quorum-sensing control.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2014년도 추계학술대회 논문집
• /
• pp.102-105
• /
• 2014

In accordance with U.S. Nuclear Regulatory Commission regulatory guide(NRC RG) 1.20(Rev.3), we are writing a comprehensive vibration assessment program(CVAP) design control document(DCD) and a technical report for U.S. NRC design certification(DC) of an Advanced Power Reactor 1400(APR1400) nuclear power plant(NPP). CVAP of an APR1400 NPP for U.S. NRC DC is classified as a non-prototype category 1 type. Therefore, CVAP DCD of reactor vessel internals(RVI) and steam generator internals(SGI) consist of analysis and full inspection program. However, piping system of primary and secondary system will be described as measurement program.