• Title/Summary/Keyword: Region-Based Method

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Molecular authentication of Lepidii seu Descurainiae Semen by the development of matK amplification primers and analysis of sequences (matK 증폭용 primer 개발 및 염기서열 분석을 통한 정력자(葶藶子) 유전자 감별)

  • Moon, Byeong Cheol;Kim, Wook Jin;Yang, Sungyu;Park, Inkyu;Yeo, Sang Min;Noh, Pureum
    • The Korea Journal of Herbology
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    • v.33 no.3
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    • pp.25-35
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    • 2018
  • Objectives : Lepidii seu Descurainiae Semen has been frequently adulterated with the seeds of several inauthentic plant species. However, the accurate identification of these plant seeds is very difficult. To develop a reliable genetic authentication tool for Lepidii seu Descurainiae Semen, we analyzed matK sequence. Methods : To obtain the matK sequences of plant materials, genomic DNA was extracted from 24 samples and PCR amplification was carried out using matK-AF/matK-8R universal primer set and matK-LDSF/matK-LDSR primer set. For identifying species-specific nucleotides and phylogenetic analysis, matK regions were sequenced and comparatively analyzed by the ClustalW and Maximum Likelihood method. Results : We developed a new primer set to amplify matK region in Lepidii seu Descurainiae Semen and closely related plant samples. From the comparative analysis of matK sequences, we identified species-specific marker nucleotides for D. sophia, L. apetalum, L. latifolium, E. cheiranthoides, E. macilentum, and D. nemorosa, respectively. Furthermore, phylogenetic analysis revealed clear classification depending on the species. These results indicated that the matK sequence obtained a new primer set in this study was useful to identify Lepidii seu Descurainiae Semen in species level. Conclusions : We developed a primer set and identified species-specific marker nucleotides enough to distinguish authentic Lepidii seu Descurainiae Semen and adulterants at the species level based on the matK sequences. These genetic tool will be useful to prevent adulteration and to standardize the quality of Lepidii seu Descurainiae Semen.

Corneal Ulcer Region Detection With Semantic Segmentation Using Deep Learning

  • Im, Jinhyuk;Kim, Daewon
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.9
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    • pp.1-12
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    • 2022
  • Traditional methods of measuring corneal ulcers were difficult to present objective basis for diagnosis because of the subjective judgment of the medical staff through photographs taken with special equipment. In this paper, we propose a method to detect the ulcer area on a pixel basis in corneal ulcer images using a semantic segmentation model. In order to solve this problem, we performed the experiment to detect the ulcer area based on the DeepLab model which has the highest performance in semantic segmentation model. For the experiment, the training and test data were selected and the backbone network of DeepLab model which set as Xception and ResNet, respectively were evaluated and compared the performances. We used Dice similarity coefficient and IoU value as an indicator to evaluate the performances. Experimental results show that when 'crop & resized' images are added to the dataset, it segment the ulcer area with an average accuracy about 93% of Dice similarity coefficient on the DeepLab model with ResNet101 as the backbone network. This study shows that the semantic segmentation model used for object detection also has an ability to make significant results when classifying objects with irregular shapes such as corneal ulcers. Ultimately, we will perform the extension of datasets and experiment with adaptive learning methods through future studies so that they can be implemented in real medical diagnosis environment.

A Study on the Development of IoT Inspection System for Gas Leakage Inspection in Kitchen Gas Range Built-in Method (주방 가스레인지 빌트인 방식에서 가스 누출검사를 위한 IoT 검사 시스템 개발에 관한 연구)

  • Kang, Dae Guk;Choi, Young Gyu
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.15 no.4
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    • pp.283-290
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    • 2022
  • In this study, an IoT inspection system that can be linked with a server was developed using a gas timer and ESP-01 Wi-Fi module installed on a gas valve in the home. The server environment of the gas leak IoT inspection system was installed with APM (Apache, PHP, MySQL) to collect gas pressure data by generation so that leakage checks could be performed. In order to control the gas leak IoT inspection system, the app inventory was used to manage the gas leak check value in real time. In addition, user convenience has been enhanced so that membership management, WiFi settings, and leakage check values can be checked through mobile apps. In order to manage subscribers by region, the user list was checked by logging in in in the administrator mode so that the information on whether or not the leak test was conducted and the results could be provided. In addition, when the user presses the gas leak check button, the pressure is automatically checked, and the measured value is stored in the server, and when a gas leak occurs, the leakage check is performed after alarm and repair so that it can be used if normal. In addition, in order to prevent overlapping membership, membership management can be performed based on MAC addresses.

Seismic damage evaluation of steel reinforced recycled concrete filled circular steel tube composite columns

  • Hui, Ma;Xiyang, Liu;Yunchong, Chen;Yanli, Zhao
    • Earthquakes and Structures
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    • v.23 no.5
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    • pp.445-462
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    • 2022
  • To investigate and evaluate the seismic damage behaviors of steel reinforced recycled concrete (SRRC) filled circular steel tube composite columns, in this study, the cyclic loading tests of 11 composite columns was carried out by using the load-displacement joint control method. The seismic damage process, hysteretic curves and performance indexes of composite columns were observed and obtained. The effects of replacement rates of recycled coarse aggregate (RCA), diameter thickness ratio, axial compression ratio, profile steel ratio and section form of profile steel on the seismic damage behaviors of composite columns were also analyzed in detail. The results show that the failure model of columns is a typical bending failure under the combined action of horizontal loads and vertical loads, and the columns have good energy dissipation capacity and ductility. In addition, the replacement rates of RCA have a certain adverse effect on the seismic bearing capacity, energy consumption and ductility of columns. The seismic damage characteristics of composite columns are revealed according to the failure modes and hysteretic curves. A modified Park-Ang seismic damage model based on the maximum displacement and cumulative energy consumption was proposed, which can consider the adverse effect of RAC on the seismic damage of columns. On this basis, the performance levels of composite columns are divided into five categories, The interlayer displacement angle and damage index are used as the damage quantitative indicators of composite columns, and the displacement angle limits of composite columns at different performance levels under 80% assurance rate are calculated as 1/105, 1/85, 1/65, 1/28, and 1/25 respectively. On this basis, the damage index limits corresponding to each performance level are calculated as 0.045, 0.1, 0.48, 0.8, and 1.0 respectively. Finally, the corresponding relations among the performance levels, damage degrees, interlayer displacement angles and damage indexes of composite columns are established. The conclusions can provide reference for the seismic design of SRRC filled circular steel tube composite columns, it fills the vacancy in the research on seismic damage of steel reinforced recycled concrete (SRRC) filled circular steel tube composite columns.

A Study on Information System for Safe Transportation of Emergency Patients in the Era of Pandemic Infectious Disease (팬데믹 감염병 시대에 안전이송을 위한 정보시스템 연구)

  • Seungyong Kim;Incheol Hwang;Dongsik Kim
    • Journal of the Society of Disaster Information
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    • v.18 no.4
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    • pp.839-846
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    • 2022
  • Purpose: To secure the safety of firefighters who are dispatched to emergency activities for patients with suspected infectious diseases during an epidemic, and to identify the current status of suspected infectious disease patients by region based on the information collected at the site, and manage firefighting infectious diseases that can be controlled and supported I want to develop a system. Method: Develop a smartphone app that can classify suspected infectious disease patients to check whether an infectious disease is suspected, and develop a disposable NFC tag for patient identification to prevent infection from suspected infectious disease patients. Develop a management system that collects and analyzes data related to emergency patients with suspected infectious disease input from the field and provides them to relevant business personnel to evaluate whether the transport of emergency patients with suspected infectious disease is improved. Result: As a result of the experiment, it was possible to determine whether an infectious disease was suspected through the algorithm implemented in the smartphone app, and the retransfer rate was significantly reduced by transferring to an appropriate hospital. Conclusion: Through this study, the possibility of improving emergency medical services by applying ICT technology to emergency medical services was confirmed. It is expected that the safety of paramedics will be actively secured.

Study on the Evaluation of Ship Collision Risk based on the Dempster-Shafer Theory (Dempster-Shafer 이론 기반의 선박충돌위험성 평가에 관한 연구)

  • Jinwan Park;Jung Sik Jeong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.5
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    • pp.462-469
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    • 2023
  • In this study, we propose a method for evaluating the risk of collision between ships to support determination on the risk of collision in a situation in which ships encounter each other and to prevent collision accidents. Because several uncertainties are involved in the navigation of a ship, must be considered when evaluating the risk of collision. We apply the Dempster-Shafer theory to manage this uncertainty and evaluate the collision risk of each target vessel in real time. The distance at the closest point approach (DCPA), time to the closest point approach (TCPA), distance from another vessel, relative bearing, and velocity ratio are used as evaluation factors for ship collision risk. The basic probability assignments (BPAs) calculated by membership functions for each evaluation factor are fused through the combination rule of the Dempster-Shafer theory. As a result of the experiment using automatic identification system (AIS) data collected in situations where ships actually encounter each other, the suitability of evaluation was verified. By evaluating the risk of collision in real time in encounter situations between ships, collision accidents caused by human errora can be prevented. This is expected to be used for vessel traffic service systems and collision avoidance systems for autonomous ships.

A Study on its Formation of the Ulsan Dutbeki Dance: Focusing on Local Features in the Ulsan District. (향토성에 의한 울산덧배기춤의 형상화에 관한 연구)

  • Choi, Heung-Kee
    • (The) Research of the performance art and culture
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    • no.41
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    • pp.187-218
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    • 2020
  • Ulsan Dutbeki is a local dance handed down by the Ulsan people through custom. This study was discussed on the locality of Ulsan Dutbeki. The method of this study is as follows. First of all, the perception of Dutbeki from the perspective of Ulsan's local characteristic. First, Ulsan Dutbeki is based on the local characteristic of the southeastern coastal area of the Korean peninsula. Second, Dutbeki features local characteristics of Ulsan as a military cultural area. Third, in Dutbeki, there is a local culture of Ulsan which was originated from the village Dongjeol and outdoor performances. Next, the researcher perceived Ulsan Dutbeki which had been handed down through custom and approached its shape. The origins of the shape are, firstly, the speech tone and gestures of Ulsan people. Secondly, folk plays related to worshiping martial arts and military training. Thirdly, the characteristics of the Dutbeki dance in coastal areas of Gyeongsangdo. Fourth, local custom displayed at the village festival of Ulsan. Ulsan is a region of Gyeongsang culture area and has similarity with other localities. However, this study limited its comparisons with regard to Dutbeki that were originated from the local characteristics of other regions. The results of this study recognized Ulsan Dutbeki as a local dance in Ulsan area. In other words, this study perceived Dutbeki, which had been an entertaining component of traditional lifestyle, as an intangible cultural heritage and studied the form in every conceivable way from an artistic point of view.

Development of SCAR Markers for the Discrimination of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma based on the RAPD (RAPD 분석을 통한 대황(大黃)과 종대황(種大黃) 감별용 SCAR 유전자 마커 개발)

  • Moon, Byeong-Cheol;Lee, Young-Mi;Chun, Jin-Mi;Lee, A-Young;Yoon, Tae-Sook;Cheon, Myeong-Sook;Choo, Byung-Kil;Kim, Ho-Kyoung
    • The Korea Journal of Herbology
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    • v.24 no.4
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    • pp.115-120
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    • 2009
  • Objectives : Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods : To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and multiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results : We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions : These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.

Establishment of Crowd Management Safety Measures Based on Crowd Density Risk Simulation (군중 밀집 위험도 시뮬레이션 기반의 인파 관리 안전대책 수립)

  • Hyuncheol Kim;Hyungjun Im;Seunghyun Lee;Youngbeom Ju;Soonjo Kwon
    • Journal of the Korean Society of Safety
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    • v.38 no.2
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    • pp.96-103
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    • 2023
  • Generally, human stampedes and crowd collapses occur when people press against each other, causing falls that may result in death or injury. Particularly, crowd accidents have become increasingly common since the 1990s, with an average of 380 deaths annually. For instance, in Korea, a stampede occurred during the Itaewon Halloween festival on October 29, 2022, when several people crowded onto a narrow, downhill road, which was 45 meters long and between 3.2 and 4 meters wide. Precisely, this stampede was primarily due to the excessive number of people relative to the road size. Essentially, stampedes can occur anywhere and at any time, not just at events, but also in other places where large crowds gather. More specifically, the likelihood of accidents increases when the crowd density exceeds a turbulence threshold of 5-6 /m2. Meanwhile, festivals and events, which have become more frequent and are promoted through social media, garner people from near and far to a specific location. Besides, as cities grow, the number of people gathering in one place increases. While stampedes are rare, their impact is significant, and the uncertainty associated with them is high. Currently, there is no scientific system to analyze the risk of stampedes due to crowd concentration. Consequently, to prevent such accidents, it is essential to prepare for crowd disasters that reflect social changes and regional characteristics. Hence, this study proposes using digital topographic maps and crowd-density risk simulations to develop a 3D model of the region. Specifically, the crowd density simulation allows for an analysis of the density of people walking along specific paths, which enables the prediction of danger areas and the risk of crowding. By using the simulation method in this study, it is anticipated that safety measures can be rationally established for specific situations, such as local festivals, and preparations may be made for crowd accidents in downtown areas.

A Study on Selection of Optimal Shelters according to Dam Break Scenario Based on Continuous Rainfall Event (연속호우사상기반의 댐 붕괴 시나리오에 따른 최적대피소 선정에 관한 연구)

  • Kim, Kyunghun;Lim, Jonghun;Kim, Hung Soo;Shin, Soeng Cheol
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.43 no.4
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    • pp.433-447
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    • 2023
  • There is a growing demand for the stability of existing dams due to abnormal climate and the aging of dams. Emergency Action Plans (EAPs) for reservoir or dam failure only consider a single rainfall event. Therefore, this study simulates dam failure caused by continuous rainfall events, and proposes the establishment of EAP by selecting the optimal shelters. We define a mega rainfall event scenario caused by continuous rainfall events with 500-year frequency in the Chungju Dam watershed and estimate the mega flood. The mega flood event scenario is divided into two cases: scenario A represents the flooding case caused by discharge release from a dam, while scenario B is the case of a dam break. As a result of flood inundation analysis, the flooded damage area by the scenario A is 50.06 km2 and the area by the scenario B is 6.1 times of scenario A (307.45 km2). We select optimal shelters for each administrative region in the city of Chungju, which has the highest inundation rate in the urban area. Seven shelter evaluation indicators from domestic and foreign shelter selection criteria are chosen, and Analytical Hierarchy Process (AHP) method is used to evaluate the alternatives. As a result of the optimal shelter selection, the six shelters are selected and five are schools. This study considers continuous rainfall events for inundation analysis and selection of optimal shelters. Also, the results of this study could be used as a reference for establishment of the EAP.